################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI Aor51HI AscI Asp718I AspI AviII BbeI BciVI BlpI Bpu1102I BsaHI BseAI BsiWI BspDI BspEI BssHII BstEII BstPI BstUI CelII ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I FseI FspI Hin1I KasI KpnI KspI MluI MroI MvnI NaeI NarI NgoMIV NheI NotI NruI PacI PflFI PmeI PshAI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AclI 2461 AACGTT AA^CGTT AflII 838 CTTAAG C^TTAAG AgeI 4414 ACCGGT A^CCGGT Alw44I 4169 GTGCAC G^TGCAC AocI 5399 CCTTAGG CC^T[ACGT]AGG ApaI 6388 GGGCCC GGGCC^C ApaLI 4169 GTGCAC G^TGCAC AvrII 1092 CCTAGG C^CTAGG BalI 6026 TGGCCA TGG^CCA BamHI 861 GGATCC G^GATCC BbrPI 2161 CACGTG CAC^GTG BbvCI 5947 GCTGAGG CCTCAGC(-5/-2) BcgI 1047 CGAAGCTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 6068 CCCGG CC^[GC]GG BfrI 838 CTTAAG C^TTAAG BglI 2677 GCCATAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1092 CCTAGG C^CTAGG BsaAI 2161 CACGTG [CT]AC^GT[GA] BsgI 6079 CTGCAC GTGCAG(16/14) BsiEI 4590 CGACCG CG[GA][CT]^CG BsmBI 962 CGTCTC CGTCTC(1/5) BspHI 4023 TCATGA T^CATGA BssSI 824 CACGAG CACGAG(-5/-1) Bst1107I 2543 GTATAC GTA^TAC BstBI 1045 TTCGAA TT^CGAA BstZ17I 2543 GTATAC GTA^TAC Bsu36I 5399 CCTTAGG CC^T[ACGT]AGG CfoI 313 GCGC GCG^C DsaI 2012 CCATGG C^C[GA][CT]GG Eco81I 5399 CCTTAGG CC^T[ACGT]AGG EcoRV 3737 GATATC GAT^ATC EcoT22I 3988 ATGCAT ATGCA^T HaeII 312 GGCGCT [GA]GCGC^[CT] HgaI 5593 GACGC GACGC(5/10) HhaI 313 GCGC GCG^C HinP1I 313 GCGC G^CGC MluNI 6026 TGGCCA TGG^CCA MscI 6026 TGGCCA TGG^CCA NciI 6068 CCCGG CC^[GC]GG NcoI 2012 CCATGG C^CATGG NdeI 1370 CATATG CA^TATG NsiI 3988 ATGCAT ATGCA^T NspV 1045 TTCGAA TT^CGAA PaeR7I 5066 CTCGAG C^TCGAG PinAI 4414 ACCGGT A^CCGGT PmaCI 2161 CACGTG CAC^GTG PmlI 2161 CACGTG CAC^GTG PpuMI 5204 AGGTCCT [GA]G^G[AT]CC[CT] Psp1406I 2461 AACGTT AA^CGTT RcaI 4023 TCATGA T^CATGA SfuI 1045 TTCGAA TT^CGAA XhoI 5066 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 957 GTCTAC GT^[AC][GT]AC 2543 GTATAC AhdI 1376 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5932 GACCCCCTGTC AseI 153 ATTAAT AT^TAAT 5764 ATTAAT AsnI 153 ATTAAT AT^TAAT 5764 ATTAAT Asp700I 3822 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4486 GAAAAAGTTC AspEI 1376 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5932 GACCCCCTGTC AvaI 4934 CCCGAG C^[CT]CG[GA]G 5066 CTCGAG BglII 1179 AGATCT A^GATCT 1850 AGATCT BsaBI 600 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3100 GATTTGCATC BsaI 1171 GAGACC GGTCTC(1/5) 5416 GAGACC BsaWI 4038 ACCGGA [AT]^CCGG[AT] 4414 ACCGGT BsoBI 4934 CCCGAG C^[CT]CG[GA]G 5066 CTCGAG BsrBI 5079 CCGCTC CCGCTC(-3/-3) 5507 CCGCTC BsrFI 4414 ACCGGT [GA]^CCGG[CT] 4592 ACCGGC BstXI 1117 CCACATCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2475 CCAGCAGCCTGG Cfr10I 4414 ACCGGT [GA]^CCGG[CT] 4592 ACCGGC DraI 1962 TTTAAA TTT^AAA 2320 TTTAAA EaeI 4586 TGGCCG [CT]^GGCC[GA] 6026 TGGCCA Eam1105I 1376 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5932 GACCCCCTGTC EcoNI 1092 CCTAGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1555 CCTGAGAGAGG EcoRI 2341 GAATTC G^AATTC 2718 GAATTC HpaI 2151 GTTAAC GTT^AAC 3861 GTTAAC MamI 600 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3100 GATTTGCATC PshBI 153 ATTAAT AT^TAAT 5764 ATTAAT XmnI 3822 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4486 GAAAAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbsI BbvI BclI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DraIII DrdI EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII