(Circular) (Six-base) MAPSORT of: pFN21AE2295.seq Check: 6016 from: 1 to: 11113 pFN21AE2295 11113 bp With 182 enzymes: SgfI * November 8, 2010 14:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6275 7957 8050 6275 Size: 1682 93 9338 Fragments arranged by size: 9338 1682 93 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8923 278 Size: 53 83 186 936 7387 2468 Fragments arranged by size: 7387 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2963 4549 8456 1937 Size: 1026 1586 3907 4594 Fragments arranged by size: 4594 3907 1586 1026 Acc65I G'GTAC_C Cuts at: 8434 8434 Size: 11113 AclI AA'CG_TT Cuts at: 1563 4467 9240 9613 1563 Size: 2904 4773 373 3063 Fragments arranged by size: 4773 3063 2904 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2712 4242 7455 7554 7610 7917 8313 9176 Size: 1080 1530 3213 99 56 307 396 863 Cuts at: 9176 10560 10828 1632 Size: 1384 268 1917 Fragments arranged by size: 3213 1917 1530 1384 1080 863 396 307 268 99 56 AflII C'TTAA_G Cuts at: 820 1017 2843 820 Size: 197 1826 9090 Fragments arranged by size: 9090 1826 197 AflIII A'CryG_T Cuts at: 1203 4288 5330 6689 7238 8383 10012 10989 1203 Size: 3085 1042 1359 549 1145 1629 977 1327 Fragments arranged by size: 3085 1629 1359 1327 1145 1042 977 549 AgeI A'CCGG_T Cuts at: 6419 9944 6419 Size: 3525 7588 AhdI GACnn_n'nnGTC Cuts at: 3386 7942 9842 3386 Size: 4556 1900 4657 Fragments arranged by size: 4657 4556 1900 AleI CACnn'nnGTG Cuts at: 1166 3683 3686 7790 1166 Size: 2517 3 4104 4489 Fragments arranged by size: 4489 4104 2517 3 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 11113 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2273 2305 4231 4263 4605 4637 2273 Size: 32 1926 32 342 32 8749 Fragments arranged by size: 8749 1926 342 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 4202 5158 6004 7688 8856 10428 4202 Size: 956 846 1684 1168 1572 4887 Fragments arranged by size: 4887 1684 1572 1168 956 846 ApaI G_GGCC'C Cuts at: 8397 8397 Size: 11113 ApaLI G'TGCA_C Cuts at: 6174 9170 10326 6174 Size: 2996 1156 6961 Fragments arranged by size: 6961 2996 1156 ApoI r'AATT_y Cuts at: 1493 2251 2987 3460 4331 4346 4379 4723 5005 Size: 758 736 473 871 15 33 344 282 Cuts at: 5005 5035 5585 5593 5640 6155 6184 8313 8422 Size: 30 550 8 47 515 29 2129 109 Cuts at: 8422 8648 8800 10909 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2129 2109 1697 871 758 736 550 515 473 344 282 226 152 109 47 33 30 29 15 8 AseI AT'TA_AT Cuts at: 160 1032 2159 7770 9667 160 Size: 872 1127 5611 1897 1606 Fragments arranged by size: 5611 1897 1606 1127 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11113 AvaI C'yCGr_G Cuts at: 1870 1943 6939 7071 8438 1870 Size: 73 4996 132 1367 4545 Fragments arranged by size: 4996 4545 1367 132 73 AvrII C'CTAG_G Cuts at: 3097 3097 Size: 11113 BamHI G'GATC_C Cuts at: 2866 8443 8810 2866 Size: 5577 367 5169 Fragments arranged by size: 5577 5169 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6642 7216 7673 8434 9889 Size: 325 217 627 4855 574 457 761 1455 Cuts at: 9889 618 Size: 1842 Fragments arranged by size: 4855 1842 1455 761 627 574 457 325 217 BanII G_rGCy'C Cuts at: 1391 3360 3422 3641 4421 6615 7764 8397 8432 Size: 1969 62 219 780 2194 1149 633 35 Cuts at: 8432 1391 Size: 4072 Fragments arranged by size: 4072 2194 1969 1149 780 633 219 62 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3289 3809 4107 7568 7669 928 Size: 971 1390 520 298 3461 101 4372 Fragments arranged by size: 4372 3461 1390 971 520 298 101 BbvCI CC'TCA_GC Cuts at: 7953 7953 Size: 11113 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3041 3075 9304 9338 1903 Size: 34 1104 34 6229 34 3678 Fragments arranged by size: 6229 3678 1104 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 9005 10215 9005 Size: 1210 9903 BclI T'GATC_A Cuts at: 1548 4162 4444 4564 1548 Size: 2614 282 120 8097 Fragments arranged by size: 8097 2614 282 120 BfrBI ATG'CAT Cuts at: 5995 5995 Size: 11113 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4688 9724 136 Size: 107 122 71 631 605 3016 5036 1525 Fragments arranged by size: 5036 3016 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3184 3855 11108 3184 Size: 671 7253 3189 Fragments arranged by size: 7253 3189 671 BlpI GC'TnA_GC Cuts at: 8530 8530 Size: 11113 Bme1580I G_kGCm'C Cuts at: 1790 5369 6178 6645 8074 8397 9174 10330 1790 Size: 3579 809 467 1429 323 777 1156 2573 Fragments arranged by size: 3579 2573 1429 1156 809 777 467 323 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 11113 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3146 3596 7517 Size: 416 53 83 399 24 1729 450 3921 Cuts at: 7517 8844 9802 442 Size: 1327 958 1753 Fragments arranged by size: 3921 1753 1729 1327 958 450 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11113 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4744 7180 4744 Size: 2436 8677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2256 2887 3456 3935 6882 7524 9773 1239 Size: 1017 631 569 479 2947 642 2249 2579 Fragments arranged by size: 2947 2579 2249 1017 642 631 569 479 Bpu10I CC'TnA_GC Cuts at: 2736 3354 3421 7953 2736 Size: 618 67 4532 5896 Fragments arranged by size: 5896 4532 618 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2775 4436 5055 7089 7836 9238 10103 10401 2775 Size: 1661 619 2034 747 1402 865 298 3487 Fragments arranged by size: 3487 2034 1661 1402 865 747 619 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3170 7415 9776 882 Size: 617 1671 4245 2361 2219 Fragments arranged by size: 4245 2361 2219 1671 617 BsaAI yAC'GTr Cuts at: 493 1204 4168 8904 493 Size: 711 2964 4736 2702 Fragments arranged by size: 4736 2964 2702 711 BsaBI GATnn'nnATC Cuts at: 2609 5109 11107 2609 Size: 2500 5998 2615 Fragments arranged by size: 5998 2615 2500 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 8920 9302 9937 275 Size: 53 83 186 936 7387 382 635 1451 Fragments arranged by size: 7387 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6043 6419 9546 9944 10218 10365 10776 6043 Size: 376 3127 398 274 147 411 6380 Fragments arranged by size: 6380 3127 411 398 376 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2885 2915 4231 4261 6356 Size: 30 165 30 1482 30 1316 30 2095 Cuts at: 6356 6386 7567 7597 7817 7847 1178 Size: 30 1181 30 220 30 4444 Fragments arranged by size: 4444 2095 1482 1316 1181 220 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3423 3709 3893 5902 7506 7719 7839 7970 Size: 2237 286 184 2009 1604 213 120 131 Cuts at: 7970 1186 Size: 4329 Fragments arranged by size: 4329 2237 2009 1604 286 213 184 131 120 BseYI C'CCAG_C Cuts at: 2801 5904 10316 10812 2801 Size: 3103 4412 496 3102 Fragments arranged by size: 4412 3103 3102 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 8069 1163 Size: 573 6333 4207 Fragments arranged by size: 6333 4207 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6598 8563 9324 10352 1540 Size: 275 186 4597 1965 761 1028 2301 Fragments arranged by size: 4597 2301 1965 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2755 3641 4421 6178 6558 6615 7012 8001 8109 Size: 886 780 1757 380 57 397 989 108 Cuts at: 8109 9174 9259 10330 2755 Size: 1065 85 1071 3538 Fragments arranged by size: 3538 1757 1071 1065 989 886 780 397 380 108 85 57 BsmI GAATG_Cn' Cuts at: 2805 3824 5831 5881 7891 8624 8717 2805 Size: 1019 2007 50 2010 733 93 5201 Fragments arranged by size: 5201 2010 2007 1019 733 93 50 BsmBI CGTCTCn'nnnn_ Cuts at: 2973 2973 Size: 11113 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2755 3360 3422 3641 4421 5369 6178 Size: 399 965 605 62 219 780 948 809 Cuts at: 6178 6558 6615 6645 7012 7764 8001 8074 8109 Size: 380 57 30 367 752 237 73 35 Cuts at: 8109 8397 8432 9174 9259 10330 1391 Size: 288 35 742 85 1071 2174 Fragments arranged by size: 2174 1071 965 948 809 780 752 742 605 399 380 367 288 237 219 85 73 62 57 35 35 30 BspEI T'CCGG_A Cuts at: 10776 10776 Size: 11113 BspHI T'CATG_A Cuts at: 6028 9002 10732 6028 Size: 2974 1730 6409 Fragments arranged by size: 6409 2974 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2306 3499 5962 6275 7957 8050 8468 844 Size: 1462 1193 2463 313 1682 93 418 3489 Fragments arranged by size: 3489 2463 1682 1462 1193 418 313 93 BsrBI CCG'CTC Cuts at: 1394 1823 7086 7514 9000 1394 Size: 429 5263 428 1486 3507 Fragments arranged by size: 5263 3507 1486 429 428 BsrDI GCAATG_nn' Cuts at: 65 1248 2308 3496 6011 9608 9782 65 Size: 1183 1060 1188 2515 3597 174 1396 Fragments arranged by size: 3597 2515 1396 1188 1183 1060 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6419 6597 9757 9944 1700 Size: 110 4609 178 3160 187 2869 Fragments arranged by size: 4609 3160 2869 187 178 110 BsrGI T'GTAC_A Cuts at: 96 2527 4261 4291 5338 5395 6414 6686 96 Size: 2431 1734 30 1047 57 1019 272 4523 Fragments arranged by size: 4523 2431 1734 1047 1019 272 57 30 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11113 BssSI C'ACGA_G Cuts at: 2829 9173 10185 2829 Size: 6344 1012 3757 Fragments arranged by size: 6344 3757 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3828 5116 5562 5992 7688 794 Size: 3034 1288 446 430 1696 4219 Fragments arranged by size: 4219 3034 1696 1288 446 430 BstBI TT'CG_AA Cuts at: 3051 9917 3051 Size: 6866 4247 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 11113 BstXI CCAn_nnnn'nTGG Cuts at: 3129 4487 3129 Size: 1358 9755 BstYI r'GATC_y Cuts at: 1972 2640 2866 3184 3855 4613 8443 8810 9195 Size: 668 226 318 671 758 3830 367 385 Cuts at: 9195 9212 10664 10750 10762 11108 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 3830 1977 1452 758 671 668 385 367 346 318 226 86 17 12 BstZ17I GTA'TAC Cuts at: 4550 4550 Size: 11113 Bsu36I CC'TnA_GG Cuts at: 7405 7405 Size: 11113 BtgI C'CryG_G Cuts at: 513 1065 4017 513 Size: 552 2952 7609 Fragments arranged by size: 7609 2952 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3787 5964 7298 8020 487 Size: 683 56 2561 2177 1334 722 3580 Fragments arranged by size: 3580 2561 2177 1334 722 683 56 BtsI GCAGTG_nn' Cuts at: 4392 6487 6987 7326 7394 8629 9423 9443 4392 Size: 2095 500 339 68 1235 794 20 6062 Fragments arranged by size: 6062 2095 1235 794 500 339 68 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11113 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 8083 8118 529 Size: 35 7519 35 3524 Fragments arranged by size: 7519 3524 35 35 DraI TTT'AAA Cuts at: 3969 4327 8417 3969 Size: 358 4090 6665 Fragments arranged by size: 6665 4090 358 DraIII CAC_nnn'GTG Cuts at: 2672 2748 7525 2672 Size: 76 4777 6260 Fragments arranged by size: 6260 4777 76 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2495 3293 8354 10120 809 Size: 507 1179 798 5061 1766 1802 Fragments arranged by size: 5061 1802 1766 1179 798 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6591 8031 8560 Size: 54 1425 50 275 18 4761 1440 529 Cuts at: 8560 9449 11078 8 Size: 889 1629 43 Fragments arranged by size: 4761 1629 1440 1425 889 529 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8560 1537 Size: 275 6748 4090 Fragments arranged by size: 6748 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2688 2811 3578 3727 6114 6588 7268 7870 Size: 1335 123 767 149 2387 474 680 602 Cuts at: 7870 9043 1353 Size: 1173 3423 Fragments arranged by size: 3423 2387 1335 1173 767 680 602 474 149 123 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5494 9700 10074 10220 387 Size: 1064 138 3905 4206 374 146 1280 Fragments arranged by size: 4206 3905 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3639 4419 6613 3639 Size: 780 2194 8139 Fragments arranged by size: 8139 2194 780 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2256 2712 2887 3456 3935 4242 6882 Size: 393 624 456 175 569 479 307 2640 Cuts at: 6882 7455 7524 7554 7610 7917 8313 9176 9773 Size: 573 69 30 56 307 396 863 597 Cuts at: 9773 10560 10828 1239 Size: 787 268 1524 Fragments arranged by size: 2640 1524 863 787 624 597 573 569 479 456 396 393 307 307 268 175 69 56 30 EcoNI CCTnn'n_nnAGG Cuts at: 3101 3564 3101 Size: 463 10650 EcoO109I rG'GnC_Cy Cuts at: 3166 3939 4690 7210 8393 8394 3166 Size: 773 751 2520 1183 1 5885 Fragments arranged by size: 5885 2520 1183 773 751 1 EcoRI G'AATT_C Cuts at: 4346 4723 8422 8800 4346 Size: 377 3699 378 6659 Fragments arranged by size: 6659 3699 378 377 EcoRV GAT'ATC Cuts at: 5744 5744 Size: 11113 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3035 3275 3035 Size: 240 10873 FspI TGC'GCA Cuts at: 9619 9619 Size: 11113 HaeII r_GCGC'y Cuts at: 1678 2321 10260 1678 Size: 643 7939 2531 Fragments arranged by size: 7939 2531 643 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3074 3106 3169 3201 3372 Size: 32 135 32 1502 32 63 32 171 Cuts at: 3372 3404 3765 3797 4429 4461 4742 4774 5068 Size: 32 361 32 632 32 281 32 294 Cuts at: 5068 5100 5177 5209 6651 6683 6800 6832 7070 Size: 32 77 32 1442 32 117 32 238 Cuts at: 7070 7102 7847 7879 7898 7928 7930 7960 7970 Size: 32 745 32 19 30 2 30 10 Cuts at: 7970 8002 9754 9786 9828 9860 1373 Size: 32 1752 32 42 32 2626 Fragments arranged by size: 2626 1752 1502 1442 745 632 361 294 281 238 171 135 117 77 63 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 30 30 19 10 2 HincII GTy'rAC Cuts at: 669 1657 1938 3676 4158 4228 5247 5868 8457 Size: 988 281 1738 482 70 1019 621 2589 Cuts at: 8457 8703 669 Size: 246 3079 Fragments arranged by size: 3079 2589 1738 1019 988 621 482 281 246 70 HindIII A'AGCT_T Cuts at: 748 3520 5844 7139 748 Size: 2772 2324 1295 4722 Fragments arranged by size: 4722 2772 2324 1295 HpaI GTT'AAC Cuts at: 4158 5868 8703 4158 Size: 1710 2835 6568 Fragments arranged by size: 6568 2835 1710 KpnI G_GTAC'C Cuts at: 8438 8438 Size: 11113 MfeI C'AATT_G Cuts at: 3321 5092 6453 8712 3321 Size: 1771 1361 2259 5722 Fragments arranged by size: 5722 2259 1771 1361 MluI A'CGCG_T Cuts at: 10989 10989 Size: 11113 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4962 5844 6560 8150 10227 10411 1305 Size: 3657 882 716 1590 2077 184 2007 Fragments arranged by size: 3657 2077 2007 1590 882 716 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 8033 10 Size: 54 1425 343 6201 3090 Fragments arranged by size: 6201 3090 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2421 3127 3683 3686 4121 6563 7790 Size: 648 1255 706 556 3 435 2442 1227 Cuts at: 7790 7974 8349 9071 9430 9589 518 Size: 184 375 722 359 159 2042 Fragments arranged by size: 2442 2042 1255 1227 722 706 648 556 435 375 359 184 159 3 MspA1I CmG'CkG Cuts at: 1606 2636 4307 6273 7685 8529 9206 10354 10599 Size: 1030 1671 1966 1412 844 677 1148 245 Cuts at: 10599 1606 Size: 2120 Fragments arranged by size: 2120 1966 1671 1412 1148 1030 844 677 245 NcoI C'CATG_G Cuts at: 513 1065 4017 513 Size: 552 2952 7609 Fragments arranged by size: 7609 2952 552 NdeI CA'TA_TG Cuts at: 387 3376 387 Size: 2989 8124 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11113 NotI GC'GGCC_GC Cuts at: 8560 8560 Size: 11113 NsiI A_TGCA'T Cuts at: 5997 5997 Size: 11113 NspI r_CATG'y Cuts at: 1132 1229 4292 5334 6693 8471 10016 1132 Size: 97 3063 1042 1359 1778 1545 2229 Fragments arranged by size: 3063 2229 1778 1545 1359 1042 97 PasI CC'CwG_GG Cuts at: 1348 3169 1348 Size: 1821 9292 PciI A'CATG_T Cuts at: 4288 5330 6689 10012 4288 Size: 1042 1359 3323 5389 Fragments arranged by size: 5389 3323 1359 1042 PflMI CCAn_nnn'nTGG Cuts at: 1108 3158 3998 4610 5158 5661 1108 Size: 2050 840 612 548 503 6560 Fragments arranged by size: 6560 2050 840 612 548 503 PmeI GTTT'AAAC Cuts at: 8417 8417 Size: 11113 PmlI CAC'GTG Cuts at: 4168 4168 Size: 11113 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4231 4263 9182 9214 10695 10727 4231 Size: 32 4919 32 1481 32 4617 Fragments arranged by size: 4919 4617 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 7210 7210 Size: 11113 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 11113 PsiI TTA'TAA Cuts at: 2430 3923 4377 5850 6885 7137 8683 2430 Size: 1493 454 1473 1035 252 1546 4860 Fragments arranged by size: 4860 1546 1493 1473 1035 454 252 PspOMI G'GGCC_C Cuts at: 8393 8393 Size: 11113 PstI C_TGCA'G Cuts at: 830 2635 4502 6272 8465 830 Size: 1805 1867 1770 2193 3478 Fragments arranged by size: 3478 2193 1867 1805 1770 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11113 PvuII CAG'CTG Cuts at: 2636 4307 6273 7685 2636 Size: 1671 1966 1412 6064 Fragments arranged by size: 6064 1966 1671 1412 SacI G_AGCT'C Cuts at: 3641 4421 6615 3641 Size: 780 2194 8139 Fragments arranged by size: 8139 2194 780 SalI G'TCGA_C Cuts at: 1936 8455 1936 Size: 6519 4594 SbfI CC_TGCA'GG Cuts at: 8465 8465 Size: 11113 ScaI AGT'ACT Cuts at: 9361 11073 9361 Size: 1712 9401 SfcI C'TryA_G Cuts at: 826 1045 2631 4024 4498 6268 8461 9596 10277 Size: 219 1586 1393 474 1770 2193 1135 681 Cuts at: 10277 10468 826 Size: 191 1471 Fragments arranged by size: 2193 1770 1586 1471 1393 1135 681 474 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11113 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11113 SmaI CCC'GGG Cuts at: 1872 8440 1872 Size: 6568 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2790 2843 4451 5070 7071 7104 Size: 197 926 847 53 1608 619 2001 33 Cuts at: 7104 7851 9217 10118 10380 820 Size: 747 1366 901 262 1553 Fragments arranged by size: 2001 1608 1553 1366 926 901 847 747 619 262 197 53 33 SnaBI TAC'GTA Cuts at: 493 8904 493 Size: 8411 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11113 SphI G_CATG'C Cuts at: 1132 8471 1132 Size: 7339 3774 SspI AAT'ATT Cuts at: 5 52 4339 4991 8020 9037 5 Size: 47 4287 652 3029 1017 2081 Fragments arranged by size: 4287 3029 2081 1017 652 47 StuI AGG'CCT Cuts at: 1517 2029 3047 3107 8243 1517 Size: 512 1018 60 5136 4387 Fragments arranged by size: 5136 4387 1018 512 60 StyI C'CwwG_G Cuts at: 513 1065 2097 3019 3097 3217 4017 6322 7167 Size: 552 1032 922 78 120 800 2305 845 Cuts at: 7167 8552 513 Size: 1385 3074 Fragments arranged by size: 3074 2305 1385 1032 922 845 800 552 120 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2507 3161 6435 7082 7536 9140 9157 Size: 1255 927 654 3274 647 454 1604 17 Cuts at: 9157 9310 9495 9940 325 Size: 153 185 445 1498 Fragments arranged by size: 3274 1604 1498 1255 927 654 647 454 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2527 4261 4291 Size: 274 80 33 51 1443 550 1734 30 Cuts at: 4291 5309 5338 5395 6414 6686 6753 9359 11071 Size: 1018 29 57 1019 272 67 2606 1712 Cuts at: 11071 96 Size: 138 Fragments arranged by size: 2606 1734 1712 1443 1019 1018 550 274 272 138 80 67 57 51 33 30 29 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2656 2688 2732 2764 6084 6116 7038 7070 7567 Size: 32 44 32 3320 32 922 32 497 Cuts at: 7567 7599 7817 7849 11073 11105 2656 Size: 32 218 32 3224 32 2664 Fragments arranged by size: 3320 3224 2664 922 497 218 44 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11113 XbaI T'CTAG_A Cuts at: 8449 8449 Size: 11113 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2113 6459 7174 7210 7548 2113 Size: 4346 715 36 338 5678 Fragments arranged by size: 5678 4346 715 338 36 XhoI C'TCGA_G Cuts at: 1943 7071 1943 Size: 5128 5985 XmaI C'CCGG_G Cuts at: 1870 8438 1870 Size: 6568 4545 XmnI GAAnn'nnTTC Cuts at: 5831 6495 9242 5831 Size: 664 2747 7702 Fragments arranged by size: 7702 2747 664 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8921 276 Size: 53 83 186 936 7387 2468 Fragments arranged by size: 7387 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BsiWI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI