(Linear) (Six-base) MAPSORT of: KIEE2295.seq Check: 1586 from: 1 to: 6408 KIEE2295.orf With 182 enzymes: SgfI * November 8, 2010 13:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 4270 5952 6045 6408 Size: 4270 1682 93 363 Fragments arranged by size: 4270 1682 363 93 AccI GT'mk_AC Cuts at: 0 958 2544 6408 Size: 958 1586 3864 Fragments arranged by size: 3864 1586 958 AclI AA'CG_TT Cuts at: 0 2462 6408 Size: 2462 3946 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 707 2237 5450 5549 5605 5912 6308 6408 Size: 707 1530 3213 99 56 307 396 100 Fragments arranged by size: 3213 1530 707 396 307 100 99 56 AflII C'TTAA_G Cuts at: 0 838 6408 Size: 838 5570 AflIII A'CryG_T Cuts at: 0 2283 3325 4684 5233 6378 6408 Size: 2283 1042 1359 549 1145 30 Fragments arranged by size: 2283 1359 1145 1042 549 30 AgeI A'CCGG_T Cuts at: 0 4414 6408 Size: 4414 1994 AhdI GACnn_n'nnGTC Cuts at: 0 1381 5937 6408 Size: 1381 4556 471 Fragments arranged by size: 4556 1381 471 AleI CACnn'nnGTG Cuts at: 0 1678 1681 5785 6408 Size: 1678 3 4104 623 Fragments arranged by size: 4104 1678 623 3 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 268 300 2226 2258 2600 2632 6408 Size: 268 32 1926 32 342 32 3776 Fragments arranged by size: 3776 1926 342 268 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 2197 3153 3999 5683 6408 Size: 2197 956 846 1684 725 Fragments arranged by size: 2197 1684 956 846 725 ApaI G_GGCC'C Cuts at: 0 6392 6408 Size: 6392 16 ApaLI G'TGCA_C Cuts at: 0 4169 6408 Size: 4169 2239 ApoI r'AATT_y Cuts at: 0 246 982 1455 2326 2341 2374 2718 3000 Size: 246 736 473 871 15 33 344 282 Cuts at: 3000 3030 3580 3588 3635 4150 4179 6308 6408 Size: 30 550 8 47 515 29 2129 100 Fragments arranged by size: 2129 871 736 550 515 473 344 282 246 100 47 33 30 29 15 8 AseI AT'TA_AT Cuts at: 0 154 5765 6408 Size: 154 5611 643 Fragments arranged by size: 5611 643 154 AvaI C'yCGr_G Cuts at: 0 4934 5066 6408 Size: 4934 132 1342 Fragments arranged by size: 4934 1342 132 AvrII C'CTAG_G Cuts at: 0 1092 6408 Size: 1092 5316 BamHI G'GATC_C Cuts at: 0 861 6408 Size: 861 5547 BanI G'GyrC_C Cuts at: 0 4637 5211 5668 6408 Size: 4637 574 457 740 Fragments arranged by size: 4637 740 574 457 BanII G_rGCy'C Cuts at: 0 1355 1417 1636 2416 4610 5759 6392 6408 Size: 1355 62 219 780 2194 1149 633 16 Fragments arranged by size: 2194 1355 1149 780 633 219 62 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 1284 1804 2102 5563 5664 6408 Size: 1284 520 298 3461 101 744 Fragments arranged by size: 3461 1284 744 520 298 101 BbvCI CC'TCA_GC Cuts at: 0 5948 6408 Size: 5948 460 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1036 1070 6408 Size: 1036 34 5338 Fragments arranged by size: 5338 1036 34 BclI T'GATC_A Cuts at: 0 2157 2439 2559 6408 Size: 2157 282 120 3849 Fragments arranged by size: 3849 2157 282 120 BfrBI ATG'CAT Cuts at: 0 3990 6408 Size: 3990 2418 BglI GCCn_nnn'nGGC Cuts at: 0 2683 6408 Size: 2683 3725 BglII A'GATC_T Cuts at: 0 1179 1850 6408 Size: 1179 671 4558 Fragments arranged by size: 4558 1179 671 Bme1580I G_kGCm'C Cuts at: 0 3364 4173 4640 6069 6392 6408 Size: 3364 809 467 1429 323 16 Fragments arranged by size: 3364 1429 809 467 323 16 BmrI ACTGGGnnnn_n' Cuts at: 0 1141 1591 5512 6408 Size: 1141 450 3921 896 Fragments arranged by size: 3921 1141 896 450 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2739 5175 6408 Size: 2739 2436 1233 Fragments arranged by size: 2739 2436 1233 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 251 882 1451 1930 4877 5519 6408 Size: 251 631 569 479 2947 642 889 Fragments arranged by size: 2947 889 642 631 569 479 251 Bpu10I CC'TnA_GC Cuts at: 0 731 1349 1416 5948 6408 Size: 731 618 67 4532 460 Fragments arranged by size: 4532 731 618 460 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 770 2431 3050 5084 5831 6408 Size: 770 1661 619 2034 747 577 Fragments arranged by size: 2034 1661 770 747 619 577 BsaI GGTCTCn'nnnn_ Cuts at: 0 1165 5410 6408 Size: 1165 4245 998 Fragments arranged by size: 4245 1165 998 BsaAI yAC'GTr Cuts at: 0 2163 6408 Size: 2163 4245 BsaBI GATnn'nnATC Cuts at: 0 604 3104 6408 Size: 604 2500 3304 Fragments arranged by size: 3304 2500 604 BsaWI w'CCGG_w Cuts at: 0 4038 4414 6408 Size: 4038 376 1994 Fragments arranged by size: 4038 1994 376 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 880 910 2226 2256 4351 4381 5562 5592 Size: 880 30 1316 30 2095 30 1181 30 Cuts at: 5592 5812 5842 6408 Size: 220 30 566 Fragments arranged by size: 2095 1316 1181 880 566 220 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1418 1704 1888 3897 5501 5714 5834 5965 Size: 1418 286 184 2009 1604 213 120 131 Cuts at: 5965 6408 Size: 443 Fragments arranged by size: 2009 1604 1418 443 286 213 184 131 120 BseYI C'CCAG_C Cuts at: 0 796 3899 6408 Size: 796 3103 2509 Fragments arranged by size: 3103 2509 796 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 6064 6408 Size: 6064 344 BsiEI CG_ry'CG Cuts at: 0 4593 6408 Size: 4593 1815 BsiHKAI G_wGCw'C Cuts at: 0 750 1636 2416 4173 4553 4610 5007 5996 Size: 750 886 780 1757 380 57 397 989 Cuts at: 5996 6104 6408 Size: 108 304 Fragments arranged by size: 1757 989 886 780 750 397 380 304 108 57 BsmI GAATG_Cn' Cuts at: 0 800 1819 3826 3876 5886 6408 Size: 800 1019 2007 50 2010 522 Fragments arranged by size: 2010 2007 1019 800 522 50 BsmBI CGTCTCn'nnnn_ Cuts at: 0 968 6408 Size: 968 5440 Bsp1286I G_dGCh'C Cuts at: 0 750 1355 1417 1636 2416 3364 4173 4553 Size: 750 605 62 219 780 948 809 380 Cuts at: 4553 4610 4640 5007 5759 5996 6069 6104 6392 Size: 57 30 367 752 237 73 35 288 Cuts at: 6392 6408 Size: 16 Fragments arranged by size: 948 809 780 752 750 605 380 367 288 237 219 73 62 57 35 30 16 BspHI T'CATG_A Cuts at: 0 4023 6408 Size: 4023 2385 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 301 1494 3957 4270 5952 6045 6408 Size: 301 1193 2463 313 1682 93 363 Fragments arranged by size: 2463 1682 1193 363 313 301 93 BsrBI CCG'CTC Cuts at: 0 5081 5509 6408 Size: 5081 428 899 Fragments arranged by size: 5081 899 428 BsrDI GCAATG_nn' Cuts at: 0 303 1491 4006 6408 Size: 303 1188 2515 2402 Fragments arranged by size: 2515 2402 1188 303 BsrFI r'CCGG_y Cuts at: 0 4414 4592 6408 Size: 4414 178 1816 Fragments arranged by size: 4414 1816 178 BsrGI T'GTAC_A Cuts at: 0 522 2256 2286 3333 3390 4409 4681 6408 Size: 522 1734 30 1047 57 1019 272 1727 Fragments arranged by size: 1734 1727 1047 1019 522 272 57 30 BssSI C'ACGA_G Cuts at: 0 824 6408 Size: 824 5584 BstAPI GCAn_nnn'nTGC Cuts at: 0 1823 3111 3557 3987 5683 6408 Size: 1823 1288 446 430 1696 725 Fragments arranged by size: 1823 1696 1288 725 446 430 BstBI TT'CG_AA Cuts at: 0 1046 6408 Size: 1046 5362 BstXI CCAn_nnnn'nTGG Cuts at: 0 1124 2482 6408 Size: 1124 1358 3926 Fragments arranged by size: 3926 1358 1124 BstYI r'GATC_y Cuts at: 0 635 861 1179 1850 2608 6408 Size: 635 226 318 671 758 3800 Fragments arranged by size: 3800 758 671 635 318 226 BstZ17I GTA'TAC Cuts at: 0 2545 6408 Size: 2545 3863 Bsu36I CC'TnA_GG Cuts at: 0 5400 6408 Size: 5400 1008 BtgI C'CryG_G Cuts at: 0 2012 6408 Size: 2012 4396 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1782 3959 5293 6015 6408 Size: 1782 2177 1334 722 393 Fragments arranged by size: 2177 1782 1334 722 393 BtsI GCAGTG_nn' Cuts at: 0 2387 4482 4982 5321 5389 6408 Size: 2387 2095 500 339 68 1019 Fragments arranged by size: 2387 2095 1019 500 339 68 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 6078 6113 6408 Size: 6078 35 295 Fragments arranged by size: 6078 295 35 DraI TTT'AAA Cuts at: 0 1964 2322 6408 Size: 1964 358 4086 Fragments arranged by size: 4086 1964 358 DraIII CAC_nnn'GTG Cuts at: 0 667 743 5520 6408 Size: 667 76 4777 888 Fragments arranged by size: 4777 888 667 76 DrdI GACnn_nn'nnGTC Cuts at: 0 490 1288 6349 6408 Size: 490 798 5061 59 Fragments arranged by size: 5061 798 490 59 EaeI y'GGCC_r Cuts at: 0 4586 6026 6408 Size: 4586 1440 382 Fragments arranged by size: 4586 1440 382 EarI CTCTTCn'nnn_ Cuts at: 0 683 806 1573 1722 4109 4583 5263 5865 Size: 683 123 767 149 2387 474 680 602 Cuts at: 5865 6408 Size: 543 Fragments arranged by size: 2387 767 683 680 602 543 474 149 123 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 3489 6408 Size: 3489 2919 EcoICRI GAG'CTC Cuts at: 0 1634 2414 4608 6408 Size: 1634 780 2194 1800 Fragments arranged by size: 2194 1800 1634 780 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 251 707 882 1451 1930 2237 4877 5450 Size: 251 456 175 569 479 307 2640 573 Cuts at: 5450 5519 5549 5605 5912 6308 6408 Size: 69 30 56 307 396 100 Fragments arranged by size: 2640 573 569 479 456 396 307 307 251 175 100 69 56 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 1096 1559 6408 Size: 1096 463 4849 Fragments arranged by size: 4849 1096 463 EcoO109I rG'GnC_Cy Cuts at: 0 1161 1934 2685 5205 6388 6389 6408 Size: 1161 773 751 2520 1183 1 19 Fragments arranged by size: 2520 1183 1161 773 751 19 1 EcoRI G'AATT_C Cuts at: 0 2341 2718 6408 Size: 2341 377 3690 Fragments arranged by size: 3690 2341 377 EcoRV GAT'ATC Cuts at: 0 3739 6408 Size: 3739 2669 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1030 1270 6408 Size: 1030 240 5138 Fragments arranged by size: 5138 1030 240 HaeII r_GCGC'y Cuts at: 0 316 6408 Size: 316 6092 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1069 1101 1164 1196 1367 1399 1760 1792 Size: 1069 32 63 32 171 32 361 32 Cuts at: 1792 2424 2456 2737 2769 3063 3095 3172 3204 Size: 632 32 281 32 294 32 77 32 Cuts at: 3204 4646 4678 4795 4827 5065 5097 5842 5874 Size: 1442 32 117 32 238 32 745 32 Cuts at: 5874 5893 5923 5925 5955 5965 5997 6408 Size: 19 30 2 30 10 32 411 Fragments arranged by size: 1442 1069 745 632 411 361 294 281 238 171 117 77 63 32 32 32 32 32 32 32 32 32 32 32 32 32 30 30 19 10 2 HincII GTy'rAC Cuts at: 0 1671 2153 2223 3242 3863 6408 Size: 1671 482 70 1019 621 2545 Fragments arranged by size: 2545 1671 1019 621 482 70 HindIII A'AGCT_T Cuts at: 0 1515 3839 5134 6408 Size: 1515 2324 1295 1274 Fragments arranged by size: 2324 1515 1295 1274 HpaI GTT'AAC Cuts at: 0 2153 3863 6408 Size: 2153 1710 2545 Fragments arranged by size: 2545 2153 1710 MfeI C'AATT_G Cuts at: 0 1316 3087 4448 6408 Size: 1316 1771 1361 1960 Fragments arranged by size: 1960 1771 1361 1316 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2957 3839 4555 6145 6408 Size: 2957 882 716 1590 263 Fragments arranged by size: 2957 1590 882 716 263 MscI TGG'CCA Cuts at: 0 6028 6408 Size: 6028 380 MslI CAynn'nnrTG Cuts at: 0 416 1122 1678 1681 2116 4558 5785 5969 Size: 416 706 556 3 435 2442 1227 184 Cuts at: 5969 6344 6408 Size: 375 64 Fragments arranged by size: 2442 1227 706 556 435 416 375 184 64 3 MspA1I CmG'CkG Cuts at: 0 631 2302 4268 5680 6408 Size: 631 1671 1966 1412 728 Fragments arranged by size: 1966 1671 1412 728 631 NcoI C'CATG_G Cuts at: 0 2012 6408 Size: 2012 4396 NdeI CA'TA_TG Cuts at: 0 1371 6408 Size: 1371 5037 NsiI A_TGCA'T Cuts at: 0 3992 6408 Size: 3992 2416 NspI r_CATG'y Cuts at: 0 2287 3329 4688 6408 Size: 2287 1042 1359 1720 Fragments arranged by size: 2287 1720 1359 1042 PasI CC'CwG_GG Cuts at: 0 1164 6408 Size: 1164 5244 PciI A'CATG_T Cuts at: 0 2283 3325 4684 6408 Size: 2283 1042 1359 1724 Fragments arranged by size: 2283 1724 1359 1042 PflMI CCAn_nnn'nTGG Cuts at: 0 1153 1993 2605 3153 3656 6408 Size: 1153 840 612 548 503 2752 Fragments arranged by size: 2752 1153 840 612 548 503 PmlI CAC'GTG Cuts at: 0 2163 6408 Size: 2163 4245 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2226 2258 6408 Size: 2226 32 4150 Fragments arranged by size: 4150 2226 32 PpuMI rG'GwC_Cy Cuts at: 0 5205 6408 Size: 5205 1203 PsiI TTA'TAA Cuts at: 0 425 1918 2372 3845 4880 5132 6408 Size: 425 1493 454 1473 1035 252 1276 Fragments arranged by size: 1493 1473 1276 1035 454 425 252 PspOMI G'GGCC_C Cuts at: 0 6388 6408 Size: 6388 20 PstI C_TGCA'G Cuts at: 0 630 2497 4267 6408 Size: 630 1867 1770 2141 Fragments arranged by size: 2141 1867 1770 630 PvuII CAG'CTG Cuts at: 0 631 2302 4268 5680 6408 Size: 631 1671 1966 1412 728 Fragments arranged by size: 1966 1671 1412 728 631 SacI G_AGCT'C Cuts at: 0 1636 2416 4610 6408 Size: 1636 780 2194 1798 Fragments arranged by size: 2194 1798 1636 780 SfcI C'TryA_G Cuts at: 0 626 2019 2493 4263 6408 Size: 626 1393 474 1770 2145 Fragments arranged by size: 2145 1770 1393 626 474 SmlI C'TyrA_G Cuts at: 0 785 838 2446 3065 5066 5099 5846 6408 Size: 785 53 1608 619 2001 33 747 562 Fragments arranged by size: 2001 1608 785 747 619 562 53 33 SspI AAT'ATT Cuts at: 0 2334 2986 6015 6408 Size: 2334 652 3029 393 Fragments arranged by size: 3029 2334 652 393 StuI AGG'CCT Cuts at: 0 24 1042 1102 6238 6408 Size: 24 1018 60 5136 170 Fragments arranged by size: 5136 1018 170 60 24 StyI C'CwwG_G Cuts at: 0 92 1014 1092 1212 2012 4317 5162 6408 Size: 92 922 78 120 800 2305 845 1246 Fragments arranged by size: 2305 1246 922 845 800 120 92 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 502 1156 4430 5077 5531 6408 Size: 502 654 3274 647 454 877 Fragments arranged by size: 3274 877 654 647 502 454 TatI w'GTAC_w Cuts at: 0 522 2256 2286 3304 3333 3390 4409 4681 Size: 522 1734 30 1018 29 57 1019 272 Cuts at: 4681 4748 6408 Size: 67 1660 Fragments arranged by size: 1734 1660 1019 1018 522 272 67 57 30 29 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 651 683 727 759 4079 4111 5033 5065 Size: 651 32 44 32 3320 32 922 32 Cuts at: 5065 5562 5594 5812 5844 6408 Size: 497 32 218 32 564 Fragments arranged by size: 3320 922 651 564 497 218 44 32 32 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 108 4454 5169 5205 5543 6408 Size: 108 4346 715 36 338 865 Fragments arranged by size: 4346 865 715 338 108 36 XhoI C'TCGA_G Cuts at: 0 5066 6408 Size: 5066 1342 XmnI GAAnn'nnTTC Cuts at: 0 3826 4490 6408 Size: 3826 664 1918 Fragments arranged by size: 3826 1918 664 Enzymes that do cut: AarI AccI AclI AcuI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BclI BfrBI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PasI PciI PflMI PmlI PpiI PpuMI PsiI PspOMI PstI PvuII SacI SfcI SmlI SspI StuI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AatII Acc65I AfeI AlfI AscI AsiSI BaeI BbeI BciVI BlpI BmgBI BmtI BsaHI BsiWI BspEI BssHII BstEII ClaI EagI FseI FspI FspAI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PfoI PmeI PshAI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XmaI ZraI