################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BanI BbeI BciVI BclI BfrI BglI BglII BlnI BsaBI BsaHI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraII DraIII Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoT22I FbaI FseI Hin1I HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacII SapI ScaI SfiI SfoI SfuI SgrAI SnaBI SphI SspBI SspI SwaI Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccII 274 CGCG CG^CG AlwI 1059 GATCC GGATC(4/5) AlwNI 865 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 1356 GGTCC G^G[AT]CC AviII 281 TGCGCA TGC^GCA BbrPI 1195 CACGTG CAC^GTG BbsI 1190 GTCTTC GAAGAC(2/6) BbvCI 1361 CCTCAGC CCTCAGC(-5/-2) BcgI 1366 GCAGCCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 313 GCTGAGC GC^T[ACGT]AGC Bpu1102I 313 GCTGAGC GC^T[ACGT]AGC BpuAI 1190 GTCTTC GAAGAC(2/6) BsaAI 1195 CACGTG [CT]AC^GT[GA] BsaI 232 GAGACC GGTCTC(1/5) BsiEI 59 CGGCCG CG[GA][CT]^CG BsmFI 1357 GTCCC GGGAC(10/14) BsmI 846 GCATTC GAATGC(1/-1) BspMI 278 ACCTGC ACCTGC(4/8) BstUI 274 CGCG CG^CG BstXI 179 CCAACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 313 GCTGAGC GC^T[ACGT]AGC DpnII 1059 GATC ^GATC DraI 792 TTTAAA TTT^AAA DrdI 706 GACCAGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 59 CGGCCG [CT]^GGCC[GA] EagI 59 CGGCCG C^GGCCG EclXI 59 CGGCCG C^GGCCG Eco52I 59 CGGCCG C^GGCCG EcoRV 739 GATATC GAT^ATC FspI 281 TGCGCA TGC^GCA HaeII 10 AGCGCC [GA]GCGC^[CT] HapII 1186 CCGG C^CGG HgaI 777 GCGTC GACGC(5/10) HindIII 667 AAGCTT A^AGCTT HpaII 1186 CCGG C^CGG MboI 1059 GATC ^GATC MspI 1186 CCGG C^CGG MvnI 274 CGCG CG^CG NdeI 1112 CATATG CA^TATG NdeII 1059 GATC ^GATC PleI 976 GAGTC GAGTC(4/5) PmaCI 1195 CACGTG CAC^GTG PmlI 1195 CACGTG CAC^GTG PshAI 1305 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Sau3AI 1059 GATC ^GATC SbfI 690 CCTGCAGG CCTGCA^GG SexAI 1101 ACCTGGT A^CC[AT]GGT SmaI 1185 CCCGGG CCC^GGG SmlI 903 CTTGAG C^T[CT][GA]AG SpeI 636 ACTAGT A^CTAGT Sse8387I 690 CCTGCAGG CCTGCA^GG XcmI 980 CCAGCTTAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1185 CCCGGG C^CCGGG XmaI 1185 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 84 GTCGAC GT^[AC][GT]AC 956 GTCGAC AspHI 172 GAGCTC G[AT]GC[AT]^C 430 GAGCTC AvaI 90 CTCGGG C^[CT]CG[GA]G 1185 CCCGGG BanII 172 GAGCTC G[GA]GC[CT]^C 430 GAGCTC BcnI 1185 CCCGG CC^[GC]GG 1186 CCGGG BmyI 172 GAGCTC G[AGT]GC[ACT]^C 430 GAGCTC BsgI 1022 CTGCAC GTGCAG(16/14) 1198 GTGCAG BsiHKAI 172 GAGCTC G[AT]GC[AT]^C 430 GAGCTC BsiYI 162 CCGCCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 303 CCAAGCCAGGG BslI 162 CCGCCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 303 CCAAGCCAGGG BsmBI 714 CGTCTC CGTCTC(1/5) 585 GAGACG BsoBI 90 CTCGGG C^[CT]CG[GA]G 1185 CCCGGG Bsp1286I 172 GAGCTC G[AGT]GC[ACT]^C 430 GAGCTC BsrI 540 ACTGG ACTGG(1/-1) 1309 CCAGT Cfr13I 1274 GGCCC G^G[ACGT]CC 1356 GGTCC EarI 895 GAAGAG CTCTTC(1/4) 941 GAAGAG EcoRI 328 GAATTC G^AATTC 415 GAATTC EcoT14I 746 CCATGG C^C[AT][AT]GG 1218 CCATGG FauI 37 GCGGG CCCGC(4/6) 110 GCGGG Ksp632I 895 GAAGAG CTCTTC(1/4) 941 GAAGAG MaeII 882 ACGT A^CGT 1196 ACGT NciI 1185 CCCGG CC^[GC]GG 1186 CCGGG NcoI 746 CCATGG C^CATGG 1218 CCATGG NlaIV 908 GGCTCC GG[ACGT]^[ACGT]CC 1356 GGTCCC SacI 172 GAGCTC GAGCT^C 430 GAGCTC SalI 84 GTCGAC G^TCGAC 956 GTCGAC Sau96I 1274 GGCCC G^G[ACGT]CC 1356 GGTCC StuI 1090 AGGCCT AGG^CCT 1231 AGGCCT StyI 746 CCATGG C^C[AT][AT]GG 1218 CCATGG TaqI 85 TCGA T^CGA 957 TCGA TfiI 686 GAATC G^A[AT]TC 757 GATTC TthHB8I 85 TCGA T^CGA 957 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AcsI AluI ApoI BbvI BfaI BpmI BsaJI BseRI BsmAI BssKI BstF5I BstNI Cac8I CfoI DdeI DsaI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI MaeI MaeIII MboII MnlI MseI MvaI MwoI NlaIII PstI ScrFI SfaNI SfcI Tru9I TseI Tsp45I Tsp509I TspRI