(Circular) (Six-base) MAPSORT of: pFN21AE2187.seq Check: 5138 from: 1 to: 6127 pFN21AE2187 6127 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3937 278 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2090 2962 3470 1937 Size: 153 872 508 4594 Fragments arranged by size: 4594 872 508 153 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2243 2270 2840 4190 5574 5842 1632 Size: 611 27 570 1350 1384 268 1917 Fragments arranged by size: 1917 1384 1350 611 570 268 27 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5930 AflIII A'CryG_T Cuts at: 1203 5026 6003 1203 Size: 3823 977 1327 Fragments arranged by size: 3823 1327 977 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6127 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2279 1184 Size: 1095 5032 AlwNI CAG_nnn'CTG Cuts at: 2875 3870 5442 2875 Size: 995 1572 3560 Fragments arranged by size: 3560 1572 995 ApaLI G'TGCA_C Cuts at: 4184 5340 4184 Size: 1156 4971 ApoI r'AATT_y Cuts at: 1493 2333 2361 2420 2507 3269 3436 3662 3814 Size: 840 28 59 87 762 167 226 152 Cuts at: 3814 5923 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 840 762 226 167 152 87 59 28 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 2095 3190 3452 1870 Size: 73 152 1095 262 4545 Fragments arranged by size: 4545 1095 262 152 73 BamHI G'GATC_C Cuts at: 3457 3824 3457 Size: 367 5760 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3448 4903 618 Size: 325 217 627 1661 1455 1842 Fragments arranged by size: 1842 1661 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2181 2439 3446 1391 Size: 790 258 1007 4072 Fragments arranged by size: 4072 1007 790 258 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3188 928 Size: 971 1289 3867 Fragments arranged by size: 3867 1289 971 BbvCI CC'TCA_GC Cuts at: 3367 3367 Size: 6127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3360 3394 4318 4352 1903 Size: 34 1423 34 924 34 3678 Fragments arranged by size: 3678 1423 924 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4019 5229 4019 Size: 1210 4917 BclI T'GATC_A Cuts at: 1548 1548 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4738 136 Size: 107 122 71 631 605 3066 1525 Fragments arranged by size: 3066 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6122 6122 Size: 6127 BlpI GC'TnA_GC Cuts at: 2319 3544 2319 Size: 1225 4902 Bme1580I G_kGCm'C Cuts at: 1790 4188 5344 1790 Size: 2398 1156 2573 Fragments arranged by size: 2573 2398 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6127 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3308 3858 4816 Size: 416 53 83 399 24 1891 550 958 Cuts at: 4816 442 Size: 1753 Fragments arranged by size: 1891 1753 958 550 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6127 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2856 2972 2856 Size: 116 6011 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 2255 2425 2567 2751 2897 2900 4787 Size: 953 63 170 142 184 146 3 1887 Cuts at: 4787 1239 Size: 2579 Fragments arranged by size: 2579 1887 953 184 170 146 142 63 3 Bpu10I CC'TnA_GC Cuts at: 3367 3367 Size: 6127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2929 4252 5117 5415 2929 Size: 1323 865 298 3641 Fragments arranged by size: 3641 1323 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2231 4790 882 Size: 617 732 2559 2219 Fragments arranged by size: 2559 2219 732 617 BsaAI yAC'GTr Cuts at: 493 1204 3202 3918 493 Size: 711 1998 716 2702 Fragments arranged by size: 2702 1998 716 711 BsaBI GATnn'nnATC Cuts at: 6121 6121 Size: 6127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3934 4316 4951 275 Size: 53 83 186 936 2401 382 635 1451 Fragments arranged by size: 2401 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4560 4958 5232 5379 5790 4560 Size: 398 274 147 411 4897 Fragments arranged by size: 4897 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3064 3094 3274 3304 3401 Size: 30 165 30 1661 30 180 30 97 Cuts at: 3401 3431 1178 Size: 30 3874 Fragments arranged by size: 3874 1661 180 165 97 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2189 2285 2894 3406 1186 Size: 1003 96 609 512 3907 Fragments arranged by size: 3907 1003 609 512 96 BseYI C'CCAG_C Cuts at: 3420 5330 5826 3420 Size: 1910 496 3721 Fragments arranged by size: 3721 1910 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3012 3224 1163 Size: 573 1276 212 4066 Fragments arranged by size: 4066 1276 573 212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2067 3577 4338 5366 1540 Size: 275 186 66 1510 761 1028 2301 Fragments arranged by size: 2301 1510 1028 761 275 186 66 BsiHKAI G_wGCw'C Cuts at: 2181 2439 4188 4273 5344 2181 Size: 258 1749 85 1071 2964 Fragments arranged by size: 2964 1749 1071 258 85 BsmI GAATG_Cn' Cuts at: 2851 3638 3731 2851 Size: 787 93 5247 Fragments arranged by size: 5247 787 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2584 2725 2584 Size: 141 5986 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2439 3446 4188 4273 5344 1391 Size: 399 391 258 1007 742 85 1071 2174 Fragments arranged by size: 2174 1071 1007 742 399 391 258 85 BspEI T'CCGG_A Cuts at: 5790 5790 Size: 6127 BspHI T'CATG_A Cuts at: 4016 5746 4016 Size: 1730 4397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2292 3482 844 Size: 1448 1190 3489 Fragments arranged by size: 3489 1448 1190 BsrBI CCG'CTC Cuts at: 1394 1823 4014 1394 Size: 429 2191 3507 Fragments arranged by size: 3507 2191 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4622 4796 65 Size: 1183 3374 174 1396 Fragments arranged by size: 3374 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4771 4958 1700 Size: 110 2961 187 2869 Fragments arranged by size: 2961 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 4187 5199 4187 Size: 1012 5115 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6127 BstBI TT'CG_AA Cuts at: 4931 4931 Size: 6127 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6127 BstXI CCAn_nnnn'nTGG Cuts at: 2191 2191 Size: 6127 BstYI r'GATC_y Cuts at: 1972 3457 3824 4209 4226 5678 5764 5776 6122 Size: 1485 367 385 17 1452 86 12 346 Cuts at: 6122 1972 Size: 1977 Fragments arranged by size: 1977 1485 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2751 3223 3245 3357 513 Size: 552 939 747 472 22 112 3283 Fragments arranged by size: 3283 939 747 552 472 112 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2718 487 Size: 683 56 1492 3896 Fragments arranged by size: 3896 1492 683 56 BtsI GCAGTG_nn' Cuts at: 2261 3643 4437 4457 2261 Size: 1382 794 20 3931 Fragments arranged by size: 3931 1382 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6127 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6092 DraI TTT'AAA Cuts at: 2799 3431 2799 Size: 632 5495 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2717 5134 809 Size: 507 1401 2417 1802 Fragments arranged by size: 2417 1802 1401 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 3574 4463 Size: 54 1425 50 275 18 234 1510 889 Cuts at: 4463 6092 8 Size: 1629 43 Fragments arranged by size: 1629 1510 1425 889 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2064 3574 1537 Size: 275 252 1510 4090 Fragments arranged by size: 4090 1510 275 252 EarI CTCTTCn'nnn_ Cuts at: 1353 2895 2941 4057 1353 Size: 1542 46 1116 3423 Fragments arranged by size: 3423 1542 1116 46 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2156 3318 3330 4714 5088 Size: 1064 138 435 132 1162 12 1384 374 Cuts at: 5088 5234 387 Size: 146 1280 Fragments arranged by size: 1384 1280 1162 1064 435 374 146 138 132 12 EcoICRI GAG'CTC Cuts at: 2179 2437 2179 Size: 258 5869 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2243 2255 2270 2425 2567 2751 Size: 393 560 51 12 15 155 142 184 Cuts at: 2751 2840 2897 2900 4190 4787 5574 5842 1239 Size: 89 57 3 1290 597 787 268 1524 Fragments arranged by size: 1524 1290 787 597 560 393 268 184 155 142 89 57 51 15 12 3 EcoRI G'AATT_C Cuts at: 2333 2420 3436 3814 2333 Size: 87 1016 378 4646 Fragments arranged by size: 4646 1016 378 87 EcoRV GAT'ATC Cuts at: 2746 2746 Size: 6127 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2408 2408 Size: 6127 FspI TGC'GCA Cuts at: 2288 4633 2288 Size: 2345 3782 HaeII r_GCGC'y Cuts at: 1678 2019 5274 1678 Size: 341 3255 2531 Fragments arranged by size: 3255 2531 341 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2749 2781 2966 2998 4768 Size: 32 135 32 1177 32 185 32 1770 Cuts at: 4768 4800 4842 4874 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1770 1177 185 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2091 2518 2963 3471 3717 669 Size: 988 281 153 427 445 508 246 3079 Fragments arranged by size: 3079 988 508 445 427 281 246 153 HindIII A'AGCT_T Cuts at: 748 2672 748 Size: 1924 4203 HpaI GTT'AAC Cuts at: 3717 3717 Size: 6127 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 3726 3726 Size: 6127 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5241 5425 1305 Size: 3936 184 2007 Fragments arranged by size: 3936 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4305 Fragments arranged by size: 4305 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4085 4444 4603 518 Size: 648 2919 359 159 2042 Fragments arranged by size: 2919 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3543 4220 5368 5613 1606 Size: 1937 677 1148 245 2120 Fragments arranged by size: 2120 1937 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2751 3223 513 Size: 552 939 747 472 3417 Fragments arranged by size: 3417 939 747 552 472 NdeI CA'TA_TG Cuts at: 387 3118 387 Size: 2731 3396 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6127 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 3485 5030 1132 Size: 97 2256 1545 2229 Fragments arranged by size: 2256 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6127 PciI A'CATG_T Cuts at: 5026 5026 Size: 6127 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6127 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PmlI CAC'GTG Cuts at: 3202 3202 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4196 4228 5709 5741 4196 Size: 32 1481 32 4582 Fragments arranged by size: 4582 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3314 1613 Size: 1701 4426 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PstI C_TGCA'G Cuts at: 830 2205 2268 2700 2931 3479 830 Size: 1375 63 432 231 548 3478 Fragments arranged by size: 3478 1375 548 432 231 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 SacI G_AGCT'C Cuts at: 2181 2439 2181 Size: 258 5869 SalI G'TCGA_C Cuts at: 1936 2089 2961 3469 1936 Size: 153 872 508 4594 Fragments arranged by size: 4594 872 508 153 SbfI CC_TGCA'GG Cuts at: 2700 3479 2700 Size: 779 5348 ScaI AGT'ACT Cuts at: 4375 6087 4375 Size: 1712 4415 SexAI A'CCwGG_T Cuts at: 3106 3106 Size: 6127 SfcI C'TryA_G Cuts at: 826 1045 2201 2264 2696 2927 3475 4610 5291 Size: 219 1156 63 432 231 548 1135 681 Cuts at: 5291 5482 826 Size: 191 1471 Fragments arranged by size: 1471 1156 1135 681 548 432 231 219 191 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 3192 3454 1872 Size: 1320 262 4545 Fragments arranged by size: 4545 1320 262 SmlI C'TyrA_G Cuts at: 820 1017 1943 2908 4231 5132 5394 820 Size: 197 926 965 1323 901 262 1553 Fragments arranged by size: 1553 1323 965 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 2641 152 Size: 2489 3638 SphI G_CATG'C Cuts at: 1132 3485 1132 Size: 2353 3774 SspI AAT'ATT Cuts at: 5 52 4051 5 Size: 47 3999 2081 Fragments arranged by size: 3999 2081 47 StuI AGG'CCT Cuts at: 1517 3097 3238 1517 Size: 1580 141 4406 Fragments arranged by size: 4406 1580 141 StyI C'CwwG_G Cuts at: 513 1065 2004 2751 3223 3566 513 Size: 552 939 747 472 343 3074 Fragments arranged by size: 3074 939 747 552 472 343 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3361 4154 4171 4324 4509 4954 325 Size: 1255 1781 793 17 153 185 445 1498 Fragments arranged by size: 1781 1498 1255 793 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4373 6085 96 Size: 274 80 33 51 1443 2396 1712 138 Fragments arranged by size: 2396 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3064 3096 3274 3306 3401 3433 6087 6119 3064 Size: 32 178 32 95 32 2654 32 3072 Fragments arranged by size: 3072 2654 178 95 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6127 XbaI T'CTAG_A Cuts at: 3463 3463 Size: 6127 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2992 2992 Size: 6127 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6127 XmaI C'CCGG_G Cuts at: 1870 3190 3452 1870 Size: 1320 262 4545 Fragments arranged by size: 4545 1320 262 XmnI GAAnn'nnTTC Cuts at: 4256 4256 Size: 6127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3935 276 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI