################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII BalI BanI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BlpI BmyI Bpu1102I BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AlwNI 207 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 414 AGCGCT AGC^GCT BbsI 1260 GAAGAC GAAGAC(2/6) BglII 615 AGATCT A^GATCT BpuAI 1260 GAAGAC GAAGAC(2/6) BsaAI 381 TACGTG [CT]AC^GT[GA] BsaI 564 GAGACC GGTCTC(1/5) BsiEI 399 CGATCG CG[GA][CT]^CG BsrDI 930 CATTGC GCAATG(2/0) BstAPI 471 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1287 CCATGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 415 GCGC GCG^C DdeI 65 CTCAG C^T[ACGT]AG EarI 1015 GAAGAG CTCTTC(1/4) Eco47III 414 AGCGCT AGC^GCT HaeII 414 AGCGCT [GA]GCGC^[CT] HgaI 1409 GCGTC GACGC(5/10) HhaI 415 GCGC GCG^C HinP1I 415 GCGC G^CGC Ksp632I 1015 GAAGAG CTCTTC(1/4) MslI 1288 CATGCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 1247 CATATG CA^TATG NspI 1462 ACATGC [GA]CATG^[CT] PleI 101 GAGTC GAGTC(4/5) PvuI 399 CGATCG CGAT^CG TfiI 657 GAATC G^A[AT]TC XcmI 1256 CCACGAAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AseI 1105 ATTAAT AT^TAAT 1302 ATTAAT AsnI 1105 ATTAAT AT^TAAT 1302 ATTAAT AvrII 1212 CCTAGG C^CTAGG 1332 CCTAGG BciVI 1082 GGATAC GTATCC(6/5) 1458 GGATAC BlnI 1212 CCTAGG C^CTAGG 1332 CCTAGG BpmI 608 CTCCAG CTGGAG(16/14) 269 CTGGAG BsiYI 19 CCTGACGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1342 CCCATCGTTGG BslI 19 CCTGACGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1342 CCCATCGTTGG Bsp1407I 107 TGTACA T^GTACA 1505 TGTACA BsrGI 107 TGTACA T^GTACA 1505 TGTACA BssKI 155 CCTGG ^CC[ACGT]GG 168 CCTGG BstNI 155 CCTGG CC^[AT]GG 168 CCTGG DraI 534 TTTAAA TTT^AAA 901 TTTAAA EcoRII 155 CCTGG ^CC[AT]GG 168 CCTGG EcoT14I 1212 CCTAGG C^C[AT][AT]GG 1332 CCTAGG HinfI 101 GAGTC G^A[ACGT]TC 657 GAATC MvaI 155 CCTGG CC^[AT]GG 168 CCTGG MwoI 471 GCAGAGGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1153 GCCTTGTGTGC PshBI 1105 ATTAAT AT^TAAT 1302 ATTAAT PstI 204 CTGCAG CTGCA^G 469 CTGCAG ScrFI 155 CCTGG CC^[ACGT]GG 168 CCTGG SspBI 107 TGTACA T^GTACA 1505 TGTACA StuI 273 AGGCCT AGG^CCT 408 AGGCCT StyI 1212 CCTAGG C^C[AT][AT]GG 1332 CCTAGG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AfaI AluI AlwI AvaII BamHI BbvI BfaI BsaJI BsmAI BsrI BstF5I BstYI Cac8I Cfr13I DpnII Fnu4HI FokI HaeIII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I SfaNI SfcI Tru9I TseI Tsp45I Tsp509I TspRI XhoII