(Circular) (Six-base) MAPSORT of: pFN21AE2771.seq Check: 4849 from: 1 to: 6232 pFN21AE2771 6232 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4042 278 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3575 1937 Size: 1638 4594 Acc65I G'GTAC_C Cuts at: 3553 3553 Size: 6232 AclI AA'CG_TT Cuts at: 1563 4359 4732 1563 Size: 2796 373 3063 Fragments arranged by size: 3063 2796 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2239 4295 5679 5947 1632 Size: 607 2056 1384 268 1917 Fragments arranged by size: 2056 1917 1384 607 268 AfeI AGC'GCT Cuts at: 2421 2421 Size: 6232 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6035 AflIII A'CryG_T Cuts at: 1203 5131 6108 1203 Size: 3928 977 1327 Fragments arranged by size: 3928 1327 977 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 4961 4961 Size: 6232 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6232 AlwNI CAG_nnn'CTG Cuts at: 2217 3975 5547 2217 Size: 1758 1572 2902 Fragments arranged by size: 2902 1758 1572 ApaLI G'TGCA_C Cuts at: 4289 5445 4289 Size: 1156 5076 ApoI r'AATT_y Cuts at: 1493 3541 3767 3919 6028 1493 Size: 2048 226 152 2109 1697 Fragments arranged by size: 2109 2048 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3111 3308 4786 160 Size: 872 2079 197 1478 1606 Fragments arranged by size: 2079 1606 1478 872 197 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 3557 1870 Size: 73 1614 4545 Fragments arranged by size: 4545 1614 73 AvrII C'CTAG_G Cuts at: 3217 3337 3217 Size: 120 6112 BamHI G'GATC_C Cuts at: 2087 2168 3213 3562 3929 2087 Size: 81 1045 349 367 4390 Fragments arranged by size: 4390 1045 367 349 81 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3553 5008 618 Size: 325 217 627 1766 1455 1842 Fragments arranged by size: 1842 1766 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3551 1391 Size: 2160 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3272 928 Size: 971 1373 3888 Fragments arranged by size: 3888 1373 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4423 4457 1903 Size: 34 2486 34 3678 Fragments arranged by size: 3678 2486 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3081 3457 4124 5334 3081 Size: 376 667 1210 3979 Fragments arranged by size: 3979 1210 667 376 BclI T'GATC_A Cuts at: 1548 1548 Size: 6232 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4843 136 Size: 107 122 71 631 605 3171 1525 Fragments arranged by size: 3171 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2620 6227 2620 Size: 3607 2625 BlpI GC'TnA_GC Cuts at: 3649 3649 Size: 6232 Bme1580I G_kGCm'C Cuts at: 1790 4293 5449 1790 Size: 2503 1156 2573 Fragments arranged by size: 2573 2503 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6232 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3963 4921 442 Size: 416 53 83 399 24 2546 958 1753 Fragments arranged by size: 2546 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2295 2598 4892 1239 Size: 1056 303 2294 2579 Fragments arranged by size: 2579 2294 1056 303 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4357 5222 5520 4357 Size: 865 298 5069 Fragments arranged by size: 5069 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2563 4895 882 Size: 617 1064 2332 2219 Fragments arranged by size: 2332 2219 1064 617 BsaAI yAC'GTr Cuts at: 493 1204 2388 4023 493 Size: 711 1184 1635 2702 Fragments arranged by size: 2702 1635 1184 711 BsaBI GATnn'nnATC Cuts at: 6226 6226 Size: 6232 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4039 4421 5056 275 Size: 53 83 186 936 2506 382 635 1451 Fragments arranged by size: 2506 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4665 5063 5337 5484 5895 4665 Size: 398 274 147 411 5002 Fragments arranged by size: 5002 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6007 Fragments arranged by size: 6007 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6232 BseYI C'CCAG_C Cuts at: 2040 3421 5435 5931 2040 Size: 1381 2014 496 2341 Fragments arranged by size: 2341 2014 1381 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5659 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2407 3682 4443 5471 1540 Size: 275 186 406 1275 761 1028 2301 Fragments arranged by size: 2301 1275 1028 761 406 275 186 BsiHKAI G_wGCw'C Cuts at: 4293 4378 5449 4293 Size: 85 1071 5076 Fragments arranged by size: 5076 1071 85 BsmI GAATG_Cn' Cuts at: 3743 3836 3743 Size: 93 6139 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3551 4293 4378 5449 1391 Size: 399 1761 742 85 1071 2174 Fragments arranged by size: 2174 1761 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5895 5895 Size: 6232 BspHI T'CATG_A Cuts at: 4121 5851 4121 Size: 1730 4502 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3587 844 Size: 2743 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4119 1394 Size: 429 2296 3507 Fragments arranged by size: 3507 2296 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2934 4727 4901 65 Size: 1183 1686 1793 174 1396 Fragments arranged by size: 1793 1686 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4876 5063 1700 Size: 110 3066 187 2869 Fragments arranged by size: 3066 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2112 3510 96 Size: 2016 1398 2818 Fragments arranged by size: 2818 2016 1398 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6232 BssSI C'ACGA_G Cuts at: 4292 5304 4292 Size: 1012 5220 BstAPI GCAn_nnn'nTGC Cuts at: 794 2482 794 Size: 1688 4544 BstBI TT'CG_AA Cuts at: 5036 5036 Size: 6232 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6232 BstXI CCAn_nnnn'nTGG Cuts at: 3299 3299 Size: 6232 BstYI r'GATC_y Cuts at: 1972 2087 2168 2620 3213 3562 3929 4314 4331 Size: 115 81 452 593 349 367 385 17 Cuts at: 4331 5783 5869 5881 6227 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 593 452 385 367 349 346 115 86 81 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4741 Fragments arranged by size: 4741 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5493 Fragments arranged by size: 5493 683 56 BtsI GCAGTG_nn' Cuts at: 2367 3748 4542 4562 2367 Size: 1381 794 20 4037 Fragments arranged by size: 4037 1381 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6197 DraI TTT'AAA Cuts at: 2541 2908 3536 2541 Size: 367 628 5237 Fragments arranged by size: 5237 628 367 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5239 809 Size: 507 3923 1802 Fragments arranged by size: 3923 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3679 4568 6197 Size: 54 1425 50 275 18 1849 889 1629 Cuts at: 6197 8 Size: 43 Fragments arranged by size: 1849 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3679 1537 Size: 275 1867 4090 Fragments arranged by size: 4090 1867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3015 4162 1353 Size: 1662 1147 3423 Fragments arranged by size: 3423 1662 1147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4819 5193 5339 387 Size: 1064 138 3230 374 146 1280 Fragments arranged by size: 3230 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2239 2295 2598 4295 4892 5679 5947 Size: 393 607 56 303 1697 597 787 268 Cuts at: 5947 1239 Size: 1524 Fragments arranged by size: 1697 1524 787 607 597 393 303 268 56 EcoRI G'AATT_C Cuts at: 3541 3919 3541 Size: 378 5854 FspI TGC'GCA Cuts at: 4738 4738 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 2423 5379 1678 Size: 745 2956 2531 Fragments arranged by size: 2956 2531 745 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2621 2653 3333 3365 4873 Size: 32 135 32 1049 32 680 32 1508 Cuts at: 4873 4905 4947 4979 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1508 1049 680 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3576 3822 669 Size: 988 281 1638 246 3079 Fragments arranged by size: 3079 1638 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6232 HpaI GTT'AAC Cuts at: 3822 3822 Size: 6232 KpnI G_GTAC'C Cuts at: 3557 3557 Size: 6232 MfeI C'AATT_G Cuts at: 3831 3831 Size: 6232 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2083 2146 3521 5346 5530 1305 Size: 778 63 1375 1825 184 2007 Fragments arranged by size: 2007 1825 1375 778 184 63 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4410 Fragments arranged by size: 4410 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3297 4190 4549 4708 518 Size: 648 2131 893 359 159 2042 Fragments arranged by size: 2131 2042 893 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3648 4325 5473 5718 1606 Size: 2042 677 1148 245 2120 Fragments arranged by size: 2120 2042 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4741 Fragments arranged by size: 4741 939 552 NdeI CA'TA_TG Cuts at: 387 3253 387 Size: 2866 3366 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6232 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NspI r_CATG'y Cuts at: 1132 1229 3471 3590 5135 1132 Size: 97 2242 119 1545 2229 Fragments arranged by size: 2242 2229 1545 119 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6232 PciI A'CATG_T Cuts at: 5131 5131 Size: 6232 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6232 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4301 4333 5814 5846 4301 Size: 32 1481 32 4687 Fragments arranged by size: 4687 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6232 PsiI TTA'TAA Cuts at: 3802 3802 Size: 6232 PstI C_TGCA'G Cuts at: 830 2213 2478 3584 830 Size: 1383 265 1106 3478 Fragments arranged by size: 3478 1383 1106 265 PvuI CG_AT'CG Cuts at: 2001 2407 2001 Size: 406 5826 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SbfI CC_TGCA'GG Cuts at: 3584 3584 Size: 6232 ScaI AGT'ACT Cuts at: 4480 6192 4480 Size: 1712 4520 SfcI C'TryA_G Cuts at: 826 1045 2209 2474 2698 3580 4715 5396 5587 Size: 219 1164 265 224 882 1135 681 191 Cuts at: 5587 826 Size: 1471 Fragments arranged by size: 1471 1164 1135 882 681 265 224 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 3559 1872 Size: 1687 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4336 5237 5499 820 Size: 197 926 2393 901 262 1553 Fragments arranged by size: 2393 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4023 493 Size: 3530 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6232 SphI G_CATG'C Cuts at: 1132 3590 1132 Size: 2458 3774 SspI AAT'ATT Cuts at: 5 52 4156 5 Size: 47 4104 2081 Fragments arranged by size: 4104 2081 47 StuI AGG'CCT Cuts at: 1517 2280 2415 1517 Size: 763 135 5334 Fragments arranged by size: 5334 763 135 StyI C'CwwG_G Cuts at: 513 1065 2004 3217 3337 3671 513 Size: 552 939 1213 120 334 3074 Fragments arranged by size: 3074 1213 939 552 334 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2032 2516 3361 4259 4276 4429 4614 Size: 1255 452 484 845 898 17 153 185 Cuts at: 4614 5059 325 Size: 445 1498 Fragments arranged by size: 1498 1255 898 845 484 452 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2112 3510 4478 Size: 274 80 33 51 1443 135 1398 968 Cuts at: 4478 6190 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1398 968 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6192 6224 6192 Size: 32 6200 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6232 XbaI T'CTAG_A Cuts at: 3568 3568 Size: 6232 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3268 3268 Size: 6232 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6232 XmaI C'CCGG_G Cuts at: 1870 3557 1870 Size: 1687 4545 XmnI GAAnn'nnTTC Cuts at: 4361 4361 Size: 6232 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4040 276 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI