(Linear) (Six-base) MAPSORT of: KIEE2771.seq Check: 4612 from: 1 to: 1527 KIEE2771.orf With 182 enzymes: SgfI * July 3, 2020 20:21 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 234 1527 Size: 234 1293 AfeI AGC'GCT Cuts at: 0 416 1527 Size: 416 1111 AlwNI CAG_nnn'CTG Cuts at: 0 212 1527 Size: 212 1315 AseI AT'TA_AT Cuts at: 0 1106 1303 1527 Size: 1106 197 224 Fragments arranged by size: 1106 224 197 AvrII C'CTAG_G Cuts at: 0 1212 1332 1527 Size: 1212 120 195 Fragments arranged by size: 1212 195 120 BamHI G'GATC_C Cuts at: 0 82 163 1208 1527 Size: 82 81 1045 319 Fragments arranged by size: 1045 319 82 81 BbsI GAAGACnn'nnnn_ Cuts at: 0 1267 1527 Size: 1267 260 BciVI GTATCCnnnnn_n' Cuts at: 0 1076 1452 1527 Size: 1076 376 75 Fragments arranged by size: 1076 376 75 BglII A'GATC_T Cuts at: 0 615 1527 Size: 615 912 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 290 593 1527 Size: 290 303 934 Fragments arranged by size: 934 303 290 BsaI GGTCTCn'nnnn_ Cuts at: 0 558 1527 Size: 558 969 BsaAI yAC'GTr Cuts at: 0 383 1527 Size: 383 1144 BseYI C'CCAG_C Cuts at: 0 35 1416 1527 Size: 35 1381 111 Fragments arranged by size: 1381 111 35 BsiEI CG_ry'CG Cuts at: 0 402 1527 Size: 402 1125 BsrDI GCAATG_nn' Cuts at: 0 929 1527 Size: 929 598 BsrGI T'GTAC_A Cuts at: 0 107 1505 1527 Size: 107 1398 22 Fragments arranged by size: 1398 107 22 BstAPI GCAn_nnn'nTGC Cuts at: 0 477 1527 Size: 477 1050 BstXI CCAn_nnnn'nTGG Cuts at: 0 1294 1527 Size: 1294 233 BstYI r'GATC_y Cuts at: 0 82 163 615 1208 1527 Size: 82 81 452 593 319 Fragments arranged by size: 593 452 319 82 81 BtsI GCAGTG_nn' Cuts at: 0 362 1527 Size: 362 1165 DraI TTT'AAA Cuts at: 0 536 903 1527 Size: 536 367 624 Fragments arranged by size: 624 536 367 EarI CTCTTCn'nnn_ Cuts at: 0 1010 1527 Size: 1010 517 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 234 290 593 1527 Size: 234 56 303 934 Fragments arranged by size: 934 303 234 56 HaeII r_GCGC'y Cuts at: 0 418 1527 Size: 418 1109 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 616 648 1328 1360 1527 Size: 616 32 680 32 167 Fragments arranged by size: 680 616 167 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 78 141 1516 1527 Size: 78 63 1375 11 Fragments arranged by size: 1375 78 63 11 MslI CAynn'nnrTG Cuts at: 0 1292 1527 Size: 1292 235 NdeI CA'TA_TG Cuts at: 0 1248 1527 Size: 1248 279 NspI r_CATG'y Cuts at: 0 1466 1527 Size: 1466 61 PstI C_TGCA'G Cuts at: 0 208 473 1527 Size: 208 265 1054 Fragments arranged by size: 1054 265 208 PvuI CG_AT'CG Cuts at: 0 402 1527 Size: 402 1125 SfcI C'TryA_G Cuts at: 0 204 469 693 1527 Size: 204 265 224 834 Fragments arranged by size: 834 265 224 204 StuI AGG'CCT Cuts at: 0 275 410 1527 Size: 275 135 1117 Fragments arranged by size: 1117 275 135 StyI C'CwwG_G Cuts at: 0 1212 1332 1527 Size: 1212 120 195 Fragments arranged by size: 1212 195 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 27 511 1356 1527 Size: 27 484 845 171 Fragments arranged by size: 845 484 171 27 TatI w'GTAC_w Cuts at: 0 107 1505 1527 Size: 107 1398 22 Fragments arranged by size: 1398 107 22 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1263 1527 Size: 1263 264 Enzymes that do cut: AcuI AfeI AlwNI AseI AvrII BamHI BbsI BciVI BglII BpmI BsaI BsaAI BseYI BsiEI BsrDI BsrGI BstAPI BstXI BstYI BtsI DraI EarI Eco57MI HaeII Hin4I MmeI MslI NdeI NspI PstI PvuI SfcI StuI StyI TaqII TatI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvaI BaeI BanI BanII BbeI BbvCI BcgI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI