Restriction map for
pFN21AE3179
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BclI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstNI
BstXI BstYI CelII ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRII
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KpnI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvaI NcoI NdeI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI Tth111I
Van91I XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 191 GTATAC GT^[AC][GT]AC
AccII 956 CGCG CG^CG
AcyI 36 GGCGCC G[GA]^CG[CT]C
AflII 660 CTTAAG C^TTAAG
AflIII 167 ACATGT A^C[GA][CT]GT
AlwI 457 GATCC GGATC(4/5)
AocI 85 CCTGAGG CC^T[ACGT]AGG
AseI 507 ATTAAT AT^TAAT
AsnI 507 ATTAAT AT^TAAT
AvaII 59 GGACC G^G[AT]CC
BbeI 36 GGCGCC GGCGC^C
BbsI 921 GTCTTC GAAGAC(2/6)
BfrI 660 CTTAAG C^TTAAG
BpuAI 921 GTCTTC GAAGAC(2/6)
BsaHI 36 GGCGCC G[GA]^CG[CT]C
BsaJI 241 CCAAGG C^C[ACGT][ACGT]GG
BsgI 362 CTGCAC GTGCAG(16/14)
BsiEI 71 CGATCG CG[GA][CT]^CG
BspLU11I 167 ACATGT A^CATGT
Bst1107I 191 GTATAC GTA^TAC
BstBI 1229 TTCGAA TT^CGAA
BstEII 350 GGTCACC G^GT[ACGT]ACC
BstPI 350 GGTCACC G^GT[ACGT]ACC
BstUI 956 CGCG CG^CG
BstZ17I 191 GTATAC GTA^TAC
Bsu36I 85 CCTGAGG CC^T[ACGT]AGG
DraI 1059 TTTAAA TTT^AAA
EaeI 158 TGGCCG [CT]^GGCC[GA]
EarI 514 GAAGAG CTCTTC(1/4)
Eco81I 85 CCTGAGG CC^T[ACGT]AGG
EcoO65I 350 GGTCACC G^GT[ACGT]ACC
EcoRI 262 GAATTC G^AATTC
EcoT14I 241 CCAAGG C^C[AT][AT]GG
HaeII 36 GGCGCC [GA]GCGC^[CT]
HgaI 957 GCGTC GACGC(5/10)
Hin1I 36 GGCGCC G[GA]^CG[CT]C
KasI 36 GGCGCC G^GCGCC
Ksp632I 514 GAAGAG CTCTTC(1/4)
MvnI 956 CGCG CG^CG
NaeI 102 GCCGGC GCC^GGC
NarI 36 GGCGCC GG^CGCC
NgoMIV 102 GCCGGC G^CCGGC
NspV 1229 TTCGAA TT^CGAA
PshAI 842 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 507 ATTAAT AT^TAAT
PstI 709 CTGCAG CTGCA^G
PvuI 71 CGATCG CGAT^CG
ScaI 861 AGTACT AGT^ACT
SfcI 709 CTGCAG C^T[GA][CT]AG
SfoI 36 GGCGCC GGC^GCC
SfuI 1229 TTCGAA TT^CGAA
SgrAI 65 CACCGGCG C[GA]^CCGG[CT]G
SmlI 660 CTTAAG C^T[CT][GA]AG
StyI 241 CCAAGG C^C[AT][AT]GG
SwaI 1058 ATTTAAAT ATTT^AAAT
TfiI 480 GATTC G^A[AT]TC
XbaI 309 TCTAGA T^CTAGA
XcmI 1101 CCATCTTCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 42 GGGCCC GGGCC^C
246 GGGCCC
BanI 36 GGCGCC G^G[CT][GA]CC
91 GGCACC
BanII 42 GGGCCC G[GA]GC[CT]^C
246 GGGCCC
BpmI 633 CTCCAG CTGGAG(16/14)
1018 CTGGAG
BsmAI 574 GAGAC GTCTC(1/5)
631 GTCTC
BsrFI 66 ACCGGC [GA]^CCGG[CT]
102 GCCGGC
CfoI 37 GCGC GCG^C
955 GCGC
Cfr10I 66 ACCGGC [GA]^CCGG[CT]
102 GCCGGC
DpnII 72 GATC ^GATC
457 GATC
DraII 42 GGGCCCC [GA]G^G[ACGT]CC[CT]
245 GGGGCCC
EcoO109I 42 GGGCCCC [GA]G^G[ACGT]CC[CT]
245 GGGGCCC
FauI 96 CCCGC CCCGC(4/6)
106 GCGGG
HhaI 37 GCGC GCG^C
955 GCGC
HinP1I 37 GCGC G^CGC
955 GCGC
HindIII 600 AAGCTT A^AGCTT
936 AAGCTT
HphI 64 TCACC GGTGA(8/7)
352 TCACC
MboI 72 GATC ^GATC
457 GATC
MslI 202 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1057 CATTTAAATG
NdeII 72 GATC ^GATC
457 GATC
NspI 167 ACATGT [GA]CATG^[CT]
1043 GCATGT
Sau3AI 72 GATC ^GATC
457 GATC
SspI 323 AATATT AAT^ATT
335 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BcnI BfaI BmyI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
Cac8I Cfr13I DdeI Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeI MaeIII MboII MnlI MseI
MspI MwoI NciI NlaIII NlaIV
PleI RsaI Sau96I ScrFI SfaNI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I