(Circular) (Six-base) MAPSORT of: pFN21AE3179.seq Check: 5135 from: 1 to: 5953 pFN21AE3179 5953 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3763 278 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2197 3296 1937 Size: 260 1099 4594 Fragments arranged by size: 4594 1099 260 Acc65I G'GTAC_C Cuts at: 3274 3274 Size: 5953 AclI AA'CG_TT Cuts at: 1563 4080 4453 1563 Size: 2517 373 3063 Fragments arranged by size: 3063 2517 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2913 3156 4016 5400 5668 1632 Size: 1281 243 860 1384 268 1917 Fragments arranged by size: 1917 1384 1281 860 268 243 AflII C'TTAA_G Cuts at: 820 1017 2665 820 Size: 197 1648 4108 Fragments arranged by size: 4108 1648 197 AflIII A'CryG_T Cuts at: 1203 2172 4852 5829 1203 Size: 969 2680 977 1327 Fragments arranged by size: 2680 1327 977 969 AgeI A'CCGG_T Cuts at: 4784 4784 Size: 5953 AhdI GACnn_n'nnGTC Cuts at: 4682 4682 Size: 5953 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5953 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5953 AlwNI CAG_nnn'CTG Cuts at: 3696 5268 3696 Size: 1572 4381 ApaI G_GGCC'C Cuts at: 2051 2255 2051 Size: 204 5749 ApaLI G'TGCA_C Cuts at: 4010 5166 4010 Size: 1156 4797 ApoI r'AATT_y Cuts at: 1493 2267 2411 2709 3262 3488 3640 5749 1493 Size: 774 144 298 553 226 152 2109 1697 Fragments arranged by size: 2109 1697 774 553 298 226 152 144 AseI AT'TA_AT Cuts at: 160 1032 2513 4507 160 Size: 872 1481 1994 1606 Fragments arranged by size: 1994 1606 1481 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 AvaI C'yCGr_G Cuts at: 1870 1943 3278 1870 Size: 73 1335 4545 Fragments arranged by size: 4545 1335 73 BamHI G'GATC_C Cuts at: 3283 3650 3283 Size: 367 5586 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2041 2096 3274 4729 618 Size: 325 217 627 254 55 1178 1455 1842 Fragments arranged by size: 1842 1455 1178 627 325 254 217 55 BanII G_rGCy'C Cuts at: 1391 2051 2255 3272 1391 Size: 660 204 1017 4072 Fragments arranged by size: 4072 1017 660 204 BbeI G_GCGC'C Cuts at: 2045 2045 Size: 5953 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2919 928 Size: 971 1020 3962 Fragments arranged by size: 3962 1020 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4144 4178 1903 Size: 34 2207 34 3678 Fragments arranged by size: 3678 2207 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3845 5055 3845 Size: 1210 4743 BclI T'GATC_A Cuts at: 1548 1548 Size: 5953 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4564 136 Size: 107 122 71 631 605 2892 1525 Fragments arranged by size: 2892 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5948 5948 Size: 5953 BlpI GC'TnA_GC Cuts at: 3370 3370 Size: 5953 Bme1580I G_kGCm'C Cuts at: 1790 2051 2099 2148 2172 2255 4014 5170 1790 Size: 261 48 49 24 83 1759 1156 2573 Fragments arranged by size: 2573 1759 1156 261 83 49 48 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5953 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3684 4642 442 Size: 416 53 83 399 24 2267 958 1753 Fragments arranged by size: 2267 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5953 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2623 3044 4613 1239 Size: 1384 421 1569 2579 Fragments arranged by size: 2579 1569 1384 421 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4078 4943 5241 4078 Size: 865 298 4790 Fragments arranged by size: 4790 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4616 882 Size: 617 3117 2219 Fragments arranged by size: 3117 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3744 493 Size: 711 2540 2702 Fragments arranged by size: 2702 2540 711 BsaBI GATnn'nnATC Cuts at: 5947 5947 Size: 5953 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2042 3760 4142 4777 Size: 53 83 186 936 509 1718 382 635 Cuts at: 4777 275 Size: 1451 Fragments arranged by size: 1718 1451 936 635 509 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4386 4784 5058 5205 5616 4386 Size: 398 274 147 411 4723 Fragments arranged by size: 4723 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5728 Fragments arranged by size: 5728 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5953 BseYI C'CCAG_C Cuts at: 5156 5652 5156 Size: 496 5457 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2352 1163 Size: 573 616 4764 Fragments arranged by size: 4764 616 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2079 3403 4164 5192 1540 Size: 275 186 78 1324 761 1028 2301 Fragments arranged by size: 2301 1324 1028 761 275 186 78 BsiHKAI G_wGCw'C Cuts at: 4014 4099 5170 4014 Size: 85 1071 4797 Fragments arranged by size: 4797 1071 85 BsmI GAATG_Cn' Cuts at: 3464 3557 3464 Size: 93 5860 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2099 2148 2172 2255 3272 4014 Size: 399 261 48 49 24 83 1017 742 Cuts at: 4014 4099 5170 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1017 742 399 261 85 83 49 48 24 BspEI T'CCGG_A Cuts at: 5616 5616 Size: 5953 BspHI T'CATG_A Cuts at: 3842 5572 3842 Size: 1730 4223 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3308 844 Size: 2464 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3840 1394 Size: 429 2017 3507 Fragments arranged by size: 3507 2017 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4448 4622 65 Size: 1183 3200 174 1396 Fragments arranged by size: 3200 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2071 2107 4597 4784 1700 Size: 110 261 36 2490 187 2869 Fragments arranged by size: 2869 2490 261 187 110 36 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5953 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5953 BssSI C'ACGA_G Cuts at: 4013 5025 4013 Size: 1012 4941 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5953 BstBI TT'CG_AA Cuts at: 3235 4757 3235 Size: 1522 4431 BstEII G'GTnAC_C Cuts at: 1184 2355 1184 Size: 1171 4782 BstYI r'GATC_y Cuts at: 1972 3283 3650 4035 4052 5504 5590 5602 5948 Size: 1311 367 385 17 1452 86 12 346 Cuts at: 5948 1972 Size: 1977 Fragments arranged by size: 1977 1452 1311 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2198 2198 Size: 5953 Bsu36I CC'TnA_GG Cuts at: 2091 2091 Size: 5953 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4462 Fragments arranged by size: 4462 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5214 Fragments arranged by size: 5214 683 56 BtsI GCAGTG_nn' Cuts at: 2364 2723 3469 4263 4283 2364 Size: 359 746 794 20 4034 Fragments arranged by size: 4034 794 746 359 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5953 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5918 DraI TTT'AAA Cuts at: 3066 3257 3066 Size: 191 5762 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4960 809 Size: 507 3644 1802 Fragments arranged by size: 3644 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2163 3400 4289 Size: 54 1425 50 275 18 333 1237 889 Cuts at: 4289 5918 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1237 889 333 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3400 1537 Size: 275 1588 4090 Fragments arranged by size: 4090 1588 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2514 3883 1353 Size: 1161 1369 3423 Fragments arranged by size: 3423 1369 1161 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4540 4914 5060 387 Size: 1064 138 2951 374 146 1280 Fragments arranged by size: 2951 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2623 2913 3044 3156 4016 4613 5400 Size: 393 991 290 131 112 860 597 787 Cuts at: 5400 5668 1239 Size: 268 1524 Fragments arranged by size: 1524 991 860 787 597 393 290 268 131 112 EcoO109I rG'GnC_Cy Cuts at: 2048 2251 2048 Size: 203 5750 EcoRI G'AATT_C Cuts at: 2267 3262 3640 2267 Size: 995 378 4580 Fragments arranged by size: 4580 995 378 FspI TGC'GCA Cuts at: 4459 4459 Size: 5953 HaeII r_GCGC'y Cuts at: 1678 2045 5100 1678 Size: 367 3055 2531 Fragments arranged by size: 3055 2531 367 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2006 2038 2539 2571 2621 Size: 32 135 32 434 32 501 32 50 Cuts at: 2621 2653 4594 4626 4668 4700 1373 Size: 32 1941 32 42 32 2626 Fragments arranged by size: 2626 1941 501 434 135 50 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3297 3543 669 Size: 988 281 1359 246 3079 Fragments arranged by size: 3079 1359 988 281 246 HindIII A'AGCT_T Cuts at: 748 2605 2941 748 Size: 1857 336 3760 Fragments arranged by size: 3760 1857 336 HpaI GTT'AAC Cuts at: 3543 3543 Size: 5953 KasI G'GCGC_C Cuts at: 2041 2041 Size: 5953 KpnI G_GTAC'C Cuts at: 3278 3278 Size: 5953 MfeI C'AATT_G Cuts at: 3552 3552 Size: 5953 MluI A'CGCG_T Cuts at: 5829 5829 Size: 5953 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5067 5251 1305 Size: 3762 184 2007 Fragments arranged by size: 3762 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4131 Fragments arranged by size: 4131 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2211 3066 3911 4270 4429 518 Size: 648 1045 855 845 359 159 2042 Fragments arranged by size: 2042 1045 855 845 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3369 4046 5194 5439 1606 Size: 1763 677 1148 245 2120 Fragments arranged by size: 2120 1763 1148 677 245 NaeI GCC'GGC Cuts at: 2109 2109 Size: 5953 NarI GG'CG_CC Cuts at: 2042 2042 Size: 5953 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4462 Fragments arranged by size: 4462 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5953 NgoMIV G'CCGG_C Cuts at: 2107 2107 Size: 5953 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5953 NotI GC'GGCC_GC Cuts at: 3400 3400 Size: 5953 NspI r_CATG'y Cuts at: 1132 1229 2176 3052 3311 4856 1132 Size: 97 947 876 259 1545 2229 Fragments arranged by size: 2229 1545 947 876 259 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5953 PciI A'CATG_T Cuts at: 2172 4852 2172 Size: 2680 3273 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5953 PmeI GTTT'AAAC Cuts at: 3257 3257 Size: 5953 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2513 2545 4022 4054 5535 5567 2513 Size: 32 1477 32 1481 32 2899 Fragments arranged by size: 2899 1481 1477 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2851 1613 Size: 1238 4715 PsiI TTA'TAA Cuts at: 3523 3523 Size: 5953 PspOMI G'GGCC_C Cuts at: 2047 2251 2047 Size: 204 5749 PstI C_TGCA'G Cuts at: 830 2718 3305 830 Size: 1888 587 3478 Fragments arranged by size: 3478 1888 587 PvuI CG_AT'CG Cuts at: 2001 2079 2001 Size: 78 5875 SalI G'TCGA_C Cuts at: 1936 3295 1936 Size: 1359 4594 SbfI CC_TGCA'GG Cuts at: 3305 3305 Size: 5953 ScaI AGT'ACT Cuts at: 2868 4201 5913 2868 Size: 1333 1712 2908 Fragments arranged by size: 2908 1712 1333 SfcI C'TryA_G Cuts at: 826 1045 2714 3301 4436 5117 5308 826 Size: 219 1669 587 1135 681 191 1471 Fragments arranged by size: 1669 1471 1135 681 587 219 191 SfoI GGC'GCC Cuts at: 2043 2043 Size: 5953 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 SgrAI Cr'CCGG_yG Cuts at: 1700 2071 1700 Size: 371 5582 SmaI CCC'GGG Cuts at: 1872 3280 1872 Size: 1408 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2665 4057 4958 5220 820 Size: 197 926 722 1392 901 262 1553 Fragments arranged by size: 1553 1392 926 901 722 262 197 SnaBI TAC'GTA Cuts at: 493 3744 493 Size: 3251 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5953 SphI G_CATG'C Cuts at: 1132 3311 1132 Size: 2179 3774 SspI AAT'ATT Cuts at: 5 52 2330 2342 3877 5 Size: 47 2278 12 1535 2081 Fragments arranged by size: 2278 2081 1535 47 12 StuI AGG'CCT Cuts at: 1517 1517 Size: 5953 StyI C'CwwG_G Cuts at: 513 1065 2004 2246 3392 513 Size: 552 939 242 1146 3074 Fragments arranged by size: 3074 1146 939 552 242 SwaI ATTT'AAAT Cuts at: 3066 3066 Size: 5953 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3027 3980 3997 4150 4335 4780 325 Size: 1255 1447 953 17 153 185 445 1498 Fragments arranged by size: 1498 1447 1255 953 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2866 2874 3076 Size: 274 80 33 51 1443 889 8 202 Cuts at: 3076 3229 4199 5911 96 Size: 153 970 1712 138 Fragments arranged by size: 1712 1443 970 889 274 202 153 138 80 51 33 8 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5913 5945 5913 Size: 32 5921 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5953 XbaI T'CTAG_A Cuts at: 2314 3289 2314 Size: 975 4978 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3113 3113 Size: 5953 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5953 XmaI C'CCGG_G Cuts at: 1870 3278 1870 Size: 1408 4545 XmnI GAAnn'nnTTC Cuts at: 4082 4082 Size: 5953 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3761 276 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI