Restriction map for
pFN21AE9609
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstPI
BstUI CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeIII MamI MluI MroI MseI
MslI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
Tru9I Tsp45I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcsI 822 AAATTT [GA]^AATT[CT]
AfaI 749 GTAC GT^AC
AlwI 111 GATCC GGATC(4/5)
AocI 676 CCTGAGG CC^T[ACGT]AGG
ApoI 822 AAATTT [GA]^AATT[CT]
AspHI 313 GAGCAC G[AT]GC[AT]^C
AvaII 42 GGTCC G^G[AT]CC
BalI 431 TGGCCA TGG^CCA
BbsI 244 GAAGAC GAAGAC(2/6)
BcgI 723 GCAGCCGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 393 CCGGG CC^[GC]GG
BfaI 37 CTAG C^TAG
BmyI 313 GAGCAC G[AGT]GC[ACT]^C
BpuAI 244 GAAGAC GAAGAC(2/6)
BsaAI 316 CACGTA [CT]AC^GT[GA]
BseRI 88 GAGGAG GAGGAG(10/8)
BsiHKAI 313 GAGCAC G[AT]GC[AT]^C
Bsp1286I 313 GAGCAC G[AGT]GC[ACT]^C
BspMI 807 ACCTGC ACCTGC(4/8)
BsrBI 604 GAGCGG CCGCTC(-3/-3)
BsrI 462 CCAGT ACTGG(1/-1)
BssSI 249 CACGAG CACGAG(-5/-1)
Bst1107I 648 GTATAC GTA^TAC
BstF5I 195 CATCC GGATG(2/0)
BstXI 107 CCAAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 110 AGATCC [GA]^GATC[CT]
BstZ17I 648 GTATAC GTA^TAC
Bsu36I 676 CCTGAGG CC^T[ACGT]AGG
DpnII 111 GATC ^GATC
EaeI 431 TGGCCA [CT]^GGCC[GA]
Eco81I 676 CCTGAGG CC^T[ACGT]AGG
FauI 606 GCGGG CCCGC(4/6)
FokI 195 CATCC GGATG(9/13)
HapII 393 CCGG C^CGG
HpaII 393 CCGG C^CGG
MaeI 37 CTAG C^TAG
MaeII 317 ACGT A^CGT
MboI 111 GATC ^GATC
MfeI 266 CAATTG C^AATTG
MflI 110 AGATCC [GA]^GATC[CT]
MluNI 431 TGGCCA TGG^CCA
MscI 431 TGGCCA TGG^CCA
MspA1I 8 CAGCGG C[AC]G^C[GT]G
MspI 393 CCGG C^CGG
MunI 266 CAATTG C^AATTG
NciI 393 CCGGG CC^[GC]GG
NdeII 111 GATC ^GATC
NlaIV 69 GGAACC GG[ACGT]^[ACGT]CC
PpuMI 41 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 749 GTAC GT^AC
Sau3AI 111 GATC ^GATC
StuI 78 AGGCCT AGG^CCT
TfiI 699 GAATC G^A[AT]TC
XcmI 419 CCAAGAAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 110 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccI 648 GTATAC GT^[AC][GT]AC
800 GTCTAC
AlwNI 493 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
640 CAGCTTCTG
BsaI 770 GAGACC GGTCTC(1/5)
223 GGTCTC
BsmFI 538 GGGAC GGGAC(10/14)
608 GGGAC
Cfr13I 42 GGTCC G^G[ACGT]CC
348 GGCCC
DraII 41 AGGTCCT [GA]G^G[ACGT]CC[CT]
347 AGGCCCT
DsaI 434 CCATGG C^C[GA][CT]GG
761 CCATGG
EcoO109I 41 AGGTCCT [GA]G^G[ACGT]CC[CT]
347 AGGCCCT
HphI 519 GGTGA GGTGA(8/7)
341 TCACC
NcoI 434 CCATGG C^CATGG
761 CCATGG
NlaIII 435 CATG CATG^
762 CATG
Sau96I 42 GGTCC G^G[ACGT]CC
348 GGCCC
SbfI 81 CCTGCAGG CCTGCA^GG
654 CCTGCAGG
Sse8387I 81 CCTGCAGG CCTGCA^GG
654 CCTGCAGG
TaqI 227 TCGA T^CGA
732 TCGA
TspRI 213 CACTG CA[GC]TG[ACGT][ACGT]^
463 CAGTG
TthHB8I 227 TCGA T^CGA
732 TCGA
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvCI BbvI BpmI
BsaJI BsiYI BslI BsmAI BssKI
BstNI Cac8I DdeI EcoNI EcoRII
EcoT14I Fnu4HI HaeIII HinfI ItaI
MboII MnlI MvaI MwoI PleI
PstI ScrFI SfaNI SfcI StyI
TseI Tsp509I