(Circular) (Six-base) MAPSORT of: pFN21AE9609.seq Check: 5971 from: 1 to: 5536 pFN21AE9609 5536 bp With 182 enzymes: SgfI * March 28, 2012 12:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3346 278 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2654 2806 2879 1937 Size: 717 152 73 4594 Fragments arranged by size: 4594 717 152 73 Acc65I G'GTAC_C Cuts at: 2857 2857 Size: 5536 AclI AA'CG_TT Cuts at: 1563 3663 4036 1563 Size: 2100 373 3063 Fragments arranged by size: 3063 2100 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2159 2268 2321 2609 3599 4983 5251 1632 Size: 527 109 53 288 990 1384 268 1917 Fragments arranged by size: 1917 1384 990 527 288 268 109 53 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5339 AflIII A'CryG_T Cuts at: 1203 4435 5412 1203 Size: 3232 977 1327 Fragments arranged by size: 3232 1327 977 AgeI A'CCGG_T Cuts at: 4367 4367 Size: 5536 AhdI GACnn_n'nnGTC Cuts at: 4265 4265 Size: 5536 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5536 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5536 AlwNI CAG_nnn'CTG Cuts at: 2503 2650 3279 4851 2503 Size: 147 629 1572 3188 Fragments arranged by size: 3188 1572 629 147 ApaLI G'TGCA_C Cuts at: 3593 4749 3593 Size: 1156 4380 ApoI r'AATT_y Cuts at: 1493 2827 2845 3071 3223 5332 1493 Size: 1334 18 226 152 2109 1697 Fragments arranged by size: 2109 1697 1334 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 4090 160 Size: 872 3058 1606 Fragments arranged by size: 3058 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 AvaI C'yCGr_G Cuts at: 1870 1943 2861 1870 Size: 73 918 4545 Fragments arranged by size: 4545 918 73 BamHI G'GATC_C Cuts at: 2866 3233 2866 Size: 367 5169 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2857 4312 618 Size: 325 217 627 1070 1455 1842 Fragments arranged by size: 1842 1455 1070 627 325 217 BanII G_rGCy'C Cuts at: 1391 2855 1391 Size: 1464 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2256 928 Size: 971 357 4208 Fragments arranged by size: 4208 971 357 BbvCI CC'TCA_GC Cuts at: 2055 2688 2694 2055 Size: 633 6 4897 Fragments arranged by size: 4897 633 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2717 2751 3727 3761 1903 Size: 34 780 34 976 34 3678 Fragments arranged by size: 3678 976 780 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3428 4638 3428 Size: 1210 4326 BclI T'GATC_A Cuts at: 1548 1548 Size: 5536 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4147 136 Size: 107 122 71 631 605 337 2138 1525 Fragments arranged by size: 2138 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5531 5531 Size: 5536 BlpI GC'TnA_GC Cuts at: 2953 2953 Size: 5536 Bme1580I G_kGCm'C Cuts at: 1790 3597 4753 1790 Size: 1807 1156 2573 Fragments arranged by size: 2573 1807 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5536 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3267 4225 442 Size: 416 53 83 399 24 1850 958 1753 Fragments arranged by size: 1850 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5536 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2402 2627 2838 4196 1239 Size: 1163 225 211 1358 2579 Fragments arranged by size: 2579 1358 1163 225 211 Bpu10I CC'TnA_GC Cuts at: 2055 2208 2223 2280 2688 2694 2055 Size: 153 15 57 408 6 4897 Fragments arranged by size: 4897 408 153 57 15 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3661 4526 4824 3661 Size: 865 298 4373 Fragments arranged by size: 4373 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2234 2769 4199 882 Size: 617 735 535 1430 2219 Fragments arranged by size: 2219 1430 735 617 535 BsaAI yAC'GTr Cuts at: 493 1204 2323 3327 493 Size: 711 1119 1004 2702 Fragments arranged by size: 2702 1119 1004 711 BsaBI GATnn'nnATC Cuts at: 5530 5530 Size: 5536 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3343 3725 4360 275 Size: 53 83 186 936 1810 382 635 1451 Fragments arranged by size: 1810 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3969 4367 4641 4788 5199 3969 Size: 398 274 147 411 4306 Fragments arranged by size: 4306 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5311 Fragments arranged by size: 5311 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 1186 Size: 922 4614 BseYI C'CCAG_C Cuts at: 2724 4739 5235 2724 Size: 2015 496 3025 Fragments arranged by size: 3025 2015 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4963 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2986 3747 4775 1540 Size: 275 186 985 761 1028 2301 Fragments arranged by size: 2301 1028 985 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2322 3597 3682 4753 2322 Size: 1275 85 1071 3105 Fragments arranged by size: 3105 1275 1071 85 BsmI GAATG_Cn' Cuts at: 3047 3140 3047 Size: 93 5443 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2322 2855 3597 3682 4753 1391 Size: 399 532 533 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 533 532 399 85 BspEI T'CCGG_A Cuts at: 5199 5199 Size: 5536 BspHI T'CATG_A Cuts at: 3425 5155 3425 Size: 1730 3806 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2821 2891 844 Size: 1977 70 3489 Fragments arranged by size: 3489 1977 70 BsrBI CCG'CTC Cuts at: 1394 1823 2611 3423 1394 Size: 429 788 812 3507 Fragments arranged by size: 3507 812 788 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4031 4205 65 Size: 1183 2783 174 1396 Fragments arranged by size: 2783 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4180 4367 1700 Size: 110 2370 187 2869 Fragments arranged by size: 2869 2370 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5536 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5536 BssSI C'ACGA_G Cuts at: 2254 3596 4608 2254 Size: 1342 1012 3182 Fragments arranged by size: 3182 1342 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5536 BstBI TT'CG_AA Cuts at: 4340 4340 Size: 5536 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5536 BstXI CCAn_nnnn'nTGG Cuts at: 2119 2119 Size: 5536 BstYI r'GATC_y Cuts at: 1972 2115 2866 3233 3618 3635 5087 5173 5185 Size: 143 751 367 385 17 1452 86 12 Cuts at: 5185 5531 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 751 385 367 346 143 86 17 12 BstZ17I GTA'TAC Cuts at: 2655 2655 Size: 5536 Bsu36I CC'TnA_GG Cuts at: 2682 2682 Size: 5536 BtgI C'CryG_G Cuts at: 513 1065 2004 2439 2766 513 Size: 552 939 435 327 3283 Fragments arranged by size: 3283 939 552 435 327 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4797 Fragments arranged by size: 4797 683 56 BtsI GCAGTG_nn' Cuts at: 2217 3052 3846 3866 2217 Size: 835 794 20 3887 Fragments arranged by size: 3887 835 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5536 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5501 DraI TTT'AAA Cuts at: 2840 2840 Size: 5536 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4543 809 Size: 507 3227 1802 Fragments arranged by size: 3227 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2436 2983 3872 Size: 54 1425 50 275 18 606 547 889 Cuts at: 3872 5501 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 606 547 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2983 1537 Size: 275 1171 4090 Fragments arranged by size: 4090 1171 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3466 1353 Size: 2113 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 2522 4123 4497 4643 387 Size: 1064 138 453 480 1601 374 146 1280 Fragments arranged by size: 1601 1280 1064 480 453 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2268 2321 2402 2609 2627 2838 Size: 393 527 109 53 81 207 18 211 Cuts at: 2838 3599 4196 4983 5251 1239 Size: 761 597 787 268 1524 Fragments arranged by size: 1524 787 761 597 527 393 268 211 207 109 81 53 18 EcoNI CCTnn'n_nnAGG Cuts at: 2054 2090 2207 2348 2054 Size: 36 117 141 5242 Fragments arranged by size: 5242 141 117 36 EcoO109I rG'GnC_Cy Cuts at: 2047 2353 2047 Size: 306 5230 EcoRI G'AATT_C Cuts at: 2845 3223 2845 Size: 378 5158 FspI TGC'GCA Cuts at: 4042 4042 Size: 5536 HaeII r_GCGC'y Cuts at: 1678 4683 1678 Size: 3005 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2530 2562 4177 4209 4251 Size: 32 135 32 958 32 1615 32 42 Cuts at: 4251 4283 1373 Size: 32 2626 Fragments arranged by size: 2626 1615 958 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2880 3126 669 Size: 988 281 942 246 3079 Fragments arranged by size: 3079 988 942 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5536 HpaI GTT'AAC Cuts at: 3126 3126 Size: 5536 KpnI G_GTAC'C Cuts at: 2861 2861 Size: 5536 MfeI C'AATT_G Cuts at: 2271 3135 2271 Size: 864 4672 MluI A'CGCG_T Cuts at: 5412 5412 Size: 5536 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4650 4834 1305 Size: 3345 184 2007 Fragments arranged by size: 3345 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2438 10 Size: 54 1425 343 606 3108 Fragments arranged by size: 3108 1425 606 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3494 3853 4012 518 Size: 648 2328 359 159 2042 Fragments arranged by size: 2328 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 2952 3629 4777 5022 1606 Size: 409 937 677 1148 245 2120 Fragments arranged by size: 2120 1148 937 677 409 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2439 2766 513 Size: 552 939 435 327 3283 Fragments arranged by size: 3283 939 552 435 327 NdeI CA'TA_TG Cuts at: 387 387 Size: 5536 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5536 NotI GC'GGCC_GC Cuts at: 2983 2983 Size: 5536 NspI r_CATG'y Cuts at: 1132 1229 2894 4439 1132 Size: 97 1665 1545 2229 Fragments arranged by size: 2229 1665 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5536 PciI A'CATG_T Cuts at: 4435 4435 Size: 5536 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5536 PfoI T'CCnGG_A Cuts at: 2397 2397 Size: 5536 PmeI GTTT'AAAC Cuts at: 2840 2840 Size: 5536 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3605 3637 5118 5150 3605 Size: 32 1481 32 3991 Fragments arranged by size: 3991 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2047 2047 Size: 5536 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5536 PsiI TTA'TAA Cuts at: 3106 3106 Size: 5536 PstI C_TGCA'G Cuts at: 830 2038 2091 2168 2196 2499 2521 2625 2664 Size: 1208 53 77 28 303 22 104 39 Cuts at: 2664 2888 830 Size: 224 3478 Fragments arranged by size: 3478 1208 303 224 104 77 53 39 28 22 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5536 SalI G'TCGA_C Cuts at: 1936 2878 1936 Size: 942 4594 SbfI CC_TGCA'GG Cuts at: 2091 2664 2888 2091 Size: 573 224 4739 Fragments arranged by size: 4739 573 224 ScaI AGT'ACT Cuts at: 3784 5496 3784 Size: 1712 3824 SfcI C'TryA_G Cuts at: 826 1045 2034 2079 2087 2164 2192 2311 2495 Size: 219 989 45 8 77 28 119 184 Cuts at: 2495 2517 2621 2660 2884 4019 4700 4891 826 Size: 22 104 39 224 1135 681 191 1471 Fragments arranged by size: 1471 1135 989 681 224 219 191 184 119 104 77 45 39 28 22 8 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5536 SmaI CCC'GGG Cuts at: 1872 2863 1872 Size: 991 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3640 4541 4803 820 Size: 197 926 1697 901 262 1553 Fragments arranged by size: 1697 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3327 493 Size: 2834 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5536 SphI G_CATG'C Cuts at: 1132 2894 1132 Size: 1762 3774 SspI AAT'ATT Cuts at: 5 52 3460 5 Size: 47 3408 2081 Fragments arranged by size: 3408 2081 47 StuI AGG'CCT Cuts at: 1517 2085 1517 Size: 568 4968 StyI C'CwwG_G Cuts at: 513 1065 2004 2238 2349 2439 2766 2975 513 Size: 552 939 234 111 90 327 209 3074 Fragments arranged by size: 3074 939 552 327 234 209 111 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3563 3580 3733 3918 4363 325 Size: 1255 1983 17 153 185 445 1498 Fragments arranged by size: 1983 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2753 3782 5494 Size: 274 80 33 51 1443 776 1029 1712 Cuts at: 5494 96 Size: 138 Fragments arranged by size: 1712 1443 1029 776 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5496 5528 5496 Size: 32 5504 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5536 XbaI T'CTAG_A Cuts at: 2872 2872 Size: 5536 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2431 2431 Size: 5536 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5536 XmaI C'CCGG_G Cuts at: 1870 2861 1870 Size: 991 4545 XmnI GAAnn'nnTTC Cuts at: 3665 3665 Size: 5536 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3344 276 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI