(Linear) (Six-base) MAPSORT of: KIEE9609.seq Check: 4557 from: 1 to: 831 KIEE9609.orf With 182 enzymes: SgfI * March 22, 2012 18:33 .. AccI GT'mk_AC Cuts at: 0 649 801 831 Size: 649 152 30 Fragments arranged by size: 649 152 30 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 263 316 604 831 Size: 154 109 53 288 227 Fragments arranged by size: 288 227 154 109 53 AlwNI CAG_nnn'CTG Cuts at: 0 498 645 831 Size: 498 147 186 Fragments arranged by size: 498 186 147 ApoI r'AATT_y Cuts at: 0 822 831 Size: 822 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 251 831 Size: 251 580 BbvCI CC'TCA_GC Cuts at: 0 50 683 689 831 Size: 50 633 6 142 Fragments arranged by size: 633 142 50 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 712 746 831 Size: 712 34 85 Fragments arranged by size: 712 85 34 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 397 622 831 Size: 397 225 209 Fragments arranged by size: 397 225 209 Bpu10I CC'TnA_GC Cuts at: 0 50 203 218 275 683 689 831 Size: 50 153 15 57 408 6 142 Fragments arranged by size: 408 153 142 57 50 15 6 BsaI GGTCTCn'nnnn_ Cuts at: 0 229 764 831 Size: 229 535 67 Fragments arranged by size: 535 229 67 BsaAI yAC'GTr Cuts at: 0 318 831 Size: 318 513 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 103 831 Size: 103 728 BseYI C'CCAG_C Cuts at: 0 719 831 Size: 719 112 BsiHKAI G_wGCw'C Cuts at: 0 317 831 Size: 317 514 Bsp1286I G_dGCh'C Cuts at: 0 317 831 Size: 317 514 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 816 831 Size: 816 15 BsrBI CCG'CTC Cuts at: 0 606 831 Size: 606 225 BssSI C'ACGA_G Cuts at: 0 249 831 Size: 249 582 BstXI CCAn_nnnn'nTGG Cuts at: 0 114 831 Size: 114 717 BstYI r'GATC_y Cuts at: 0 110 831 Size: 110 721 BstZ17I GTA'TAC Cuts at: 0 650 831 Size: 650 181 Bsu36I CC'TnA_GG Cuts at: 0 677 831 Size: 677 154 BtgI C'CryG_G Cuts at: 0 434 761 831 Size: 434 327 70 Fragments arranged by size: 434 327 70 BtsI GCAGTG_nn' Cuts at: 0 212 831 Size: 212 619 EaeI y'GGCC_r Cuts at: 0 431 831 Size: 431 400 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 37 517 831 Size: 37 480 314 Fragments arranged by size: 480 314 37 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 263 316 397 604 622 831 Size: 154 109 53 81 207 18 209 Fragments arranged by size: 209 207 154 109 81 53 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 49 85 202 343 831 Size: 49 36 117 141 488 Fragments arranged by size: 488 141 117 49 36 EcoO109I rG'GnC_Cy Cuts at: 0 42 348 831 Size: 42 306 483 Fragments arranged by size: 483 306 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 525 557 831 Size: 525 32 274 Fragments arranged by size: 525 274 32 MfeI C'AATT_G Cuts at: 0 266 831 Size: 266 565 MscI TGG'CCA Cuts at: 0 433 831 Size: 433 398 MspA1I CmG'CkG Cuts at: 0 10 831 Size: 10 821 NcoI C'CATG_G Cuts at: 0 434 761 831 Size: 434 327 70 Fragments arranged by size: 434 327 70 PfoI T'CCnGG_A Cuts at: 0 392 831 Size: 392 439 PpuMI rG'GwC_Cy Cuts at: 0 42 831 Size: 42 789 PstI C_TGCA'G Cuts at: 0 33 86 163 191 494 516 620 659 Size: 33 53 77 28 303 22 104 39 Cuts at: 659 831 Size: 172 Fragments arranged by size: 303 172 104 77 53 39 33 28 22 SbfI CC_TGCA'GG Cuts at: 0 86 659 831 Size: 86 573 172 Fragments arranged by size: 573 172 86 SfcI C'TryA_G Cuts at: 0 29 74 82 159 187 306 490 512 Size: 29 45 8 77 28 119 184 22 Cuts at: 512 616 655 831 Size: 104 39 176 Fragments arranged by size: 184 176 119 104 77 45 39 29 28 22 8 StuI AGG'CCT Cuts at: 0 80 831 Size: 80 751 StyI C'CwwG_G Cuts at: 0 233 344 434 761 831 Size: 233 111 90 327 70 Fragments arranged by size: 327 233 111 90 70 TatI w'GTAC_w Cuts at: 0 748 831 Size: 748 83 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 426 831 Size: 426 405 Enzymes that do cut: AccI AcuI AlwNI ApoI BbsI BbvCI BcgI BpmI Bpu10I BsaI BsaAI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI BssSI BstXI BstYI BstZ17I Bsu36I BtgI BtsI EaeI EciI Eco57MI EcoNI EcoO109I Hin4I MfeI MscI MspA1I NcoI PfoI PpuMI PstI SbfI SfcI StuI StyI TatI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI MmeI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI