################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AhdI AocI AscI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaBI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HpaI HphI KasI KpnI KspI MamI MroI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 705 GACGTC GACGT^C AcsI 1115 AAATTC [GA]^AATT[CT] AcyI 705 GACGTC G[GA]^CG[CT]C AgeI 1598 ACCGGT A^CCGGT Alw44I 1002 GTGCAC G^TGCAC Aor51HI 791 AGCGCT AGC^GCT ApaI 155 GGGCCC GGGCC^C ApaLI 1002 GTGCAC G^TGCAC ApoI 1115 AAATTC [GA]^AATT[CT] AseI 601 ATTAAT AT^TAAT AsnI 601 ATTAAT AT^TAAT AspI 1060 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 339 GCTGAGG CCTCAGC(-5/-2) BlpI 837 GCTCAGC GC^T[ACGT]AGC Bpu1102I 837 GCTCAGC GC^T[ACGT]AGC BsaAI 78 CACGTA [CT]AC^GT[GA] BsaHI 705 GACGTC G[GA]^CG[CT]C BsaWI 1598 ACCGGT [AT]^CCGG[AT] BspLU11I 248 ACATGT A^CATGT BsrBI 199 GAGCGG CCGCTC(-3/-3) BstAPI 87 GCAGTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 627 GGATCT [GA]^GATC[CT] CelII 837 GCTCAGC GC^T[ACGT]AGC DraIII 100 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1174 GACTTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1634 CCACGG C^C[GA][CT]GG Eco47III 791 AGCGCT AGC^GCT EcoNI 1271 CCTTCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 791 AGCGCT [GA]GCGC^[CT] Hin1I 705 GACGTC G[GA]^CG[CT]C HincII 280 GTCAAC GT[CT]^[GA]AC HindII 280 GTCAAC GT[CT]^[GA]AC HindIII 1432 AAGCTT A^AGCTT MaeIII 1396 GTGAC ^GT[ACGT]AC MfeI 692 CAATTG C^AATTG MflI 627 GGATCT [GA]^GATC[CT] MluI 1341 ACGCGT A^CGCGT MunI 692 CAATTG C^AATTG PflFI 1060 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1598 ACCGGT A^CCGGT PshBI 601 ATTAAT AT^TAAT ScaI 233 AGTACT AGT^ACT SmaI 1538 CCCGGG CCC^GGG SmlI 1018 CTCAAG C^T[CT][GA]AG SphI 92 GCATGC GCATG^C TaqI 980 TCGA T^CGA TfiI 1328 GATTC G^A[AT]TC Tsp45I 1396 GTGAC ^GT[GC]AC Tth111I 1060 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 980 TCGA T^CGA XcmI 1487 CCATAACAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 627 GGATCT [GA]^GATC[CT] XmaCI 1538 CCCGGG C^CCGGG XmaI 1538 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflIII 248 ACATGT A^C[GA][CT]GT 1341 ACGCGT AlwI 1050 GATCC GGATC(4/5) 627 GGATC AlwNI 67 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 1421 CAGCTTCTG Asp700I 1144 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1206 GAAAACATTC AvaI 1538 CCCGGG C^[CT]CG[GA]G 1561 CCCGAG BbsI 210 GAAGAC GAAGAC(2/6) 1289 GAAGAC BglI 938 GCCGAGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1132 GCCTCCTCGGC BpuAI 210 GAAGAC GAAGAC(2/6) 1289 GAAGAC BsgI 142 GTGCAG GTGCAG(16/14) 997 GTGCAG BsmBI 613 CGTCTC CGTCTC(1/5) 941 GAGACG BsoBI 1538 CCCGGG C^[CT]CG[GA]G 1561 CCCGAG BsrFI 485 ACCGGC [GA]^CCGG[CT] 1598 ACCGGT BssSI 1863 CACGAG CACGAG(-5/-1) 748 CTCGTG Cfr10I 485 ACCGGC [GA]^CCGG[CT] 1598 ACCGGT EarI 472 CTCTTC CTCTTC(1/4) 126 GAAGAG Ksp632I 472 CTCTTC CTCTTC(1/4) 126 GAAGAG MseI 602 TTAA T^TAA 1217 TTAA NspI 92 GCATGC [GA]CATG^[CT] 248 ACATGT SacI 835 GAGCTC GAGCT^C 1072 GAGCTC SapI 126 GAAGAGC GCTCTTC(1/4) 471 GCTCTTC Tru9I 602 TTAA T^TAA 1217 TTAA XmnI 1144 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1206 GAAAACATTC ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccII AciI AfaI AluI AspHI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI MaeI MaeII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp509I TspRI Van91I