(Circular) (Six-base) MAPSORT of: pFN21AE5730.seq Check: 2726 from: 1 to: 6595 pFN21AE5730 6595 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2963 3528 2963 Size: 565 6030 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2714 4405 278 Size: 53 83 186 936 1178 1691 2468 Fragments arranged by size: 2468 1691 1178 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3938 1937 Size: 2001 4594 Acc65I G'GTAC_C Cuts at: 3916 3916 Size: 6595 AclI AA'CG_TT Cuts at: 1563 4722 5095 1563 Size: 3159 373 3063 Fragments arranged by size: 3159 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3470 4658 6042 6310 1632 Size: 1838 1188 1384 268 1917 Fragments arranged by size: 1917 1838 1384 1188 268 AfeI AGC'GCT Cuts at: 2798 2798 Size: 6595 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6398 AflIII A'CryG_T Cuts at: 1203 2253 3346 5494 6471 1203 Size: 1050 1093 2148 977 1327 Fragments arranged by size: 2148 1327 1093 1050 977 AgeI A'CCGG_T Cuts at: 3603 5426 3603 Size: 1823 4772 AhdI GACnn_n'nnGTC Cuts at: 5324 5324 Size: 6595 AleI CACnn'nnGTG Cuts at: 1166 2519 1166 Size: 1353 5242 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2558 3578 3839 1184 Size: 1374 1020 261 3940 Fragments arranged by size: 3940 1374 1020 261 AlwNI CAG_nnn'CTG Cuts at: 2077 3431 4338 5910 2077 Size: 1354 907 1572 2762 Fragments arranged by size: 2762 1572 1354 907 ApaI G_GGCC'C Cuts at: 2164 2164 Size: 6595 ApaLI G'TGCA_C Cuts at: 3007 4652 5808 3007 Size: 1645 1156 3794 Fragments arranged by size: 3794 1645 1156 ApoI r'AATT_y Cuts at: 1493 3120 3904 4130 4282 6391 1493 Size: 1627 784 226 152 2109 1697 Fragments arranged by size: 2109 1697 1627 784 226 152 AseI AT'TA_AT Cuts at: 160 1032 2607 5149 160 Size: 872 1575 2542 1606 Fragments arranged by size: 2542 1606 1575 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6595 AvaI C'yCGr_G Cuts at: 1870 1943 3543 3566 3920 1870 Size: 73 1600 23 354 4545 Fragments arranged by size: 4545 1600 354 73 23 BamHI G'GATC_C Cuts at: 3925 4292 3925 Size: 367 6228 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2330 2951 3301 3628 3686 Size: 325 217 627 543 621 350 327 58 Cuts at: 3686 3916 5371 618 Size: 230 1455 1842 Fragments arranged by size: 1842 1455 627 621 543 350 327 325 230 217 58 BanII G_rGCy'C Cuts at: 1391 2164 2478 2844 3081 3914 1391 Size: 773 314 366 237 833 4072 Fragments arranged by size: 4072 833 773 366 314 237 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2222 3301 928 Size: 971 323 1079 4222 Fragments arranged by size: 4222 1079 971 323 BbvCI CC'TCA_GC Cuts at: 2345 2345 Size: 6595 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4786 4820 1903 Size: 34 2849 34 3678 Fragments arranged by size: 3678 2849 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4487 5697 4487 Size: 1210 5385 BclI T'GATC_A Cuts at: 1548 1548 Size: 6595 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2949 3143 5206 Size: 107 122 71 631 605 1277 194 2063 Cuts at: 5206 136 Size: 1525 Fragments arranged by size: 2063 1525 1277 631 605 194 122 107 71 BglII A'GATC_T Cuts at: 6590 6590 Size: 6595 BlpI GC'TnA_GC Cuts at: 2843 4012 2843 Size: 1169 5426 Bme1580I G_kGCm'C Cuts at: 1790 2061 2164 2863 3011 3306 3469 3633 4656 Size: 271 103 699 148 295 163 164 1023 Cuts at: 4656 5812 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1023 699 295 271 164 163 148 103 BmgBI CAC'GTC Cuts at: 1318 2560 1318 Size: 1242 5353 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2236 4326 5284 Size: 416 53 83 399 24 819 2090 958 Cuts at: 5284 442 Size: 1753 Fragments arranged by size: 2090 1753 958 819 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6595 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2222 2275 2849 3251 3357 5255 1239 Size: 983 53 574 402 106 1898 2579 Fragments arranged by size: 2579 1898 983 574 402 106 53 Bpu10I CC'TnA_GC Cuts at: 2345 2553 2345 Size: 208 6387 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3008 4720 5585 5883 3008 Size: 1712 865 298 3720 Fragments arranged by size: 3720 1712 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2174 3704 3801 5258 882 Size: 617 675 1530 97 1457 2219 Fragments arranged by size: 2219 1530 1457 675 617 97 BsaAI yAC'GTr Cuts at: 493 1204 2085 4386 493 Size: 711 881 2301 2702 Fragments arranged by size: 2702 2301 881 711 BsaBI GATnn'nnATC Cuts at: 6589 6589 Size: 6595 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2711 4402 4784 5419 Size: 53 83 186 936 1178 1691 382 635 Cuts at: 5419 275 Size: 1451 Fragments arranged by size: 1691 1451 1178 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3603 5028 5426 5700 5847 6258 3603 Size: 1425 398 274 147 411 3940 Fragments arranged by size: 3940 1425 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2390 2420 2853 2883 3122 Size: 30 165 30 987 30 433 30 239 Cuts at: 3122 3152 1178 Size: 30 4621 Fragments arranged by size: 4621 987 433 239 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2132 2473 2737 3130 1186 Size: 859 87 341 264 393 4651 Fragments arranged by size: 4651 859 393 341 264 87 BseYI C'CCAG_C Cuts at: 2021 3702 3875 5798 6294 2021 Size: 1681 173 1923 496 2322 Fragments arranged by size: 2322 1923 1681 496 173 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2168 3023 1163 Size: 573 432 855 4735 Fragments arranged by size: 4735 855 573 432 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4045 4806 5834 1540 Size: 275 186 2044 761 1028 2301 Fragments arranged by size: 2301 2044 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2559 2844 3011 3081 4656 4741 5812 2559 Size: 285 167 70 1575 85 1071 3342 Fragments arranged by size: 3342 1575 1071 285 167 85 70 BsmI GAATG_Cn' Cuts at: 4106 4199 4106 Size: 93 6502 BsmBI CGTCTCn'nnnn_ Cuts at: 2624 2940 2624 Size: 316 6279 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2164 2478 2559 2844 2863 3011 Size: 399 271 103 314 81 285 19 148 Cuts at: 3011 3081 3306 3469 3633 3914 4656 4741 5812 Size: 70 225 163 164 281 742 85 1071 Cuts at: 5812 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 314 285 281 271 225 164 163 148 103 85 81 70 19 BspEI T'CCGG_A Cuts at: 6258 6258 Size: 6595 BspHI T'CATG_A Cuts at: 4484 6214 4484 Size: 1730 4865 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2097 2963 3528 3950 844 Size: 1253 866 565 422 3489 Fragments arranged by size: 3489 1253 866 565 422 BsrBI CCG'CTC Cuts at: 1394 1823 2206 4482 1394 Size: 429 383 2276 3507 Fragments arranged by size: 3507 2276 429 383 BsrDI GCAATG_nn' Cuts at: 65 1248 5090 5264 65 Size: 1183 3842 174 1396 Fragments arranged by size: 3842 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2490 3603 5239 5426 1700 Size: 110 680 1113 1636 187 2869 Fragments arranged by size: 2869 1636 1113 680 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6595 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6595 BssSI C'ACGA_G Cuts at: 2753 3868 4655 5667 2753 Size: 1115 787 1012 3681 Fragments arranged by size: 3681 1115 1012 787 BstAPI GCAn_nnn'nTGC Cuts at: 794 2098 794 Size: 1304 5291 BstBI TT'CG_AA Cuts at: 5399 5399 Size: 6595 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6595 BstYI r'GATC_y Cuts at: 1972 2632 3925 4292 4677 4694 6146 6232 6244 Size: 660 1293 367 385 17 1452 86 12 Cuts at: 6244 6590 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1293 660 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3639 513 Size: 552 939 1635 3469 Fragments arranged by size: 3469 1635 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5856 Fragments arranged by size: 5856 683 56 BtsI GCAGTG_nn' Cuts at: 2032 2099 3011 3527 4111 4905 4925 2032 Size: 67 912 516 584 794 20 3702 Fragments arranged by size: 3702 912 794 584 516 67 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6595 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3762 3797 529 Size: 35 3198 35 3327 Fragments arranged by size: 3327 3198 35 35 DraI TTT'AAA Cuts at: 3899 3899 Size: 6595 DraIII CAC_nnn'GTG Cuts at: 2110 2110 Size: 6595 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3185 5602 809 Size: 507 1869 2417 1802 Fragments arranged by size: 2417 1869 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2500 3366 Size: 54 1425 50 275 18 249 421 866 Cuts at: 3366 3489 3504 4042 4931 6560 8 Size: 123 15 538 889 1629 43 Fragments arranged by size: 1629 1425 889 866 538 421 275 249 123 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 4042 1537 Size: 275 2230 4090 Fragments arranged by size: 4090 2230 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2126 2483 4525 1353 Size: 773 357 2042 3423 Fragments arranged by size: 3423 2042 773 357 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2603 2895 3317 5182 5556 5702 Size: 1064 138 1014 292 422 1865 374 146 Cuts at: 5702 387 Size: 1280 Fragments arranged by size: 1865 1280 1064 1014 422 374 292 146 138 EcoICRI GAG'CTC Cuts at: 2842 3079 2842 Size: 237 6358 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 2275 2849 3251 3357 3470 4658 Size: 393 590 53 574 402 106 113 1188 Cuts at: 4658 5255 6042 6310 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1188 787 597 590 574 402 393 268 113 106 53 EcoNI CCTnn'n_nnAGG Cuts at: 3280 3280 Size: 6595 EcoO109I rG'GnC_Cy Cuts at: 2160 2161 2389 2160 Size: 1 228 6366 Fragments arranged by size: 6366 228 1 EcoRI G'AATT_C Cuts at: 3904 4282 3904 Size: 378 6217 FspI TGC'GCA Cuts at: 5101 5101 Size: 6595 HaeII r_GCGC'y Cuts at: 1678 2800 5742 1678 Size: 1122 2942 2531 Fragments arranged by size: 2942 2531 1122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2296 2328 2390 2422 3305 Size: 32 135 32 724 32 62 32 883 Cuts at: 3305 3337 5236 5268 5310 5342 1373 Size: 32 1899 32 42 32 2626 Fragments arranged by size: 2626 1899 883 724 135 62 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2287 3939 4185 669 Size: 988 281 349 1652 246 3079 Fragments arranged by size: 3079 1652 988 349 281 246 HindIII A'AGCT_T Cuts at: 748 3437 748 Size: 2689 3906 HpaI GTT'AAC Cuts at: 4185 4185 Size: 6595 KpnI G_GTAC'C Cuts at: 3920 3920 Size: 6595 MfeI C'AATT_G Cuts at: 2697 4194 2697 Size: 1497 5098 MluI A'CGCG_T Cuts at: 3346 6471 3346 Size: 3125 3470 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2697 5709 5893 1305 Size: 1392 3012 184 2007 Fragments arranged by size: 3012 2007 1392 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2081 3491 3506 10 Size: 54 1425 343 249 1410 15 3099 Fragments arranged by size: 3099 1425 1410 343 249 54 15 MslI CAynn'nnrTG Cuts at: 518 1166 2519 3257 3485 3644 3800 4553 4912 Size: 648 1353 738 228 159 156 753 359 Cuts at: 4912 5071 518 Size: 159 2042 Fragments arranged by size: 2042 1353 753 738 648 359 228 159 159 156 MspA1I CmG'CkG Cuts at: 1606 2065 2344 2437 3271 3744 4011 4688 5836 Size: 459 279 93 834 473 267 677 1148 Cuts at: 5836 6081 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 834 677 473 459 279 267 245 93 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5104 Fragments arranged by size: 5104 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6595 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6595 NotI GC'GGCC_GC Cuts at: 4042 4042 Size: 6595 NspI r_CATG'y Cuts at: 1132 1229 2101 2257 3953 5498 1132 Size: 97 872 156 1696 1545 2229 Fragments arranged by size: 2229 1696 1545 872 156 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6595 PciI A'CATG_T Cuts at: 2253 5494 2253 Size: 3241 3354 PflMI CCAn_nnn'nTGG Cuts at: 1108 2910 2987 3523 1108 Size: 1802 77 536 4180 Fragments arranged by size: 4180 1802 536 77 PfoI T'CCnGG_A Cuts at: 2199 3336 2199 Size: 1137 5458 PmeI GTTT'AAAC Cuts at: 3899 3899 Size: 6595 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2108 2140 4664 4696 6177 6209 2108 Size: 32 2524 32 1481 32 2494 Fragments arranged by size: 2524 2494 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6595 PsiI TTA'TAA Cuts at: 4165 4165 Size: 6595 PspOMI G'GGCC_C Cuts at: 2160 2160 Size: 6595 PstI C_TGCA'G Cuts at: 830 2070 2094 2190 2931 3818 3947 830 Size: 1240 24 96 741 887 129 3478 Fragments arranged by size: 3478 1240 887 741 129 96 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6595 PvuII CAG'CTG Cuts at: 2344 2437 3271 3744 2344 Size: 93 834 473 5195 Fragments arranged by size: 5195 834 473 93 SacI G_AGCT'C Cuts at: 2844 3081 2844 Size: 237 6358 SalI G'TCGA_C Cuts at: 1936 3937 1936 Size: 2001 4594 SapI GCTCTTCn'nnn_ Cuts at: 2126 2483 2126 Size: 357 6238 SbfI CC_TGCA'GG Cuts at: 3947 3947 Size: 6595 ScaI AGT'ACT Cuts at: 2240 4843 6555 2240 Size: 2603 1712 2280 Fragments arranged by size: 2603 2280 1712 SfcI C'TryA_G Cuts at: 826 1045 2066 2090 2186 2887 2927 3280 3814 Size: 219 1021 24 96 701 40 353 534 Cuts at: 3814 3943 5078 5759 5950 826 Size: 129 1135 681 191 1471 Fragments arranged by size: 1471 1135 1021 701 681 534 353 219 191 129 96 40 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6595 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6595 SmaI CCC'GGG Cuts at: 1872 3545 3922 1872 Size: 1673 377 4545 Fragments arranged by size: 4545 1673 377 SmlI C'TyrA_G Cuts at: 820 1017 1943 3023 4699 5600 5862 820 Size: 197 926 1080 1676 901 262 1553 Fragments arranged by size: 1676 1553 1080 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4386 493 Size: 3893 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6595 SphI G_CATG'C Cuts at: 1132 2101 3953 1132 Size: 969 1852 3774 Fragments arranged by size: 3774 1852 969 SrfI GCCC'GGGC Cuts at: 3545 3545 Size: 6595 SspI AAT'ATT Cuts at: 5 52 4519 5 Size: 47 4467 2081 Fragments arranged by size: 4467 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6595 StyI C'CwwG_G Cuts at: 513 1065 2004 4034 513 Size: 552 939 2030 3074 Fragments arranged by size: 3074 2030 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2218 2867 4622 4639 4792 4977 5422 Size: 1255 638 649 1755 17 153 185 445 Cuts at: 5422 325 Size: 1498 Fragments arranged by size: 1755 1498 1255 649 638 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2238 2374 4841 Size: 274 80 33 51 1443 261 136 2467 Cuts at: 4841 6553 96 Size: 1712 138 Fragments arranged by size: 2467 1712 1443 274 261 138 136 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2853 2885 3122 3154 6555 6587 2853 Size: 32 237 32 3401 32 2861 Fragments arranged by size: 3401 2861 237 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3068 1316 Size: 1752 4843 XbaI T'CTAG_A Cuts at: 3931 3931 Size: 6595 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3499 3499 Size: 6595 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6595 XmaI C'CCGG_G Cuts at: 1870 3543 3920 1870 Size: 1673 377 4545 Fragments arranged by size: 4545 1673 377 XmnI GAAnn'nnTTC Cuts at: 3153 3215 4724 3153 Size: 62 1509 5024 Fragments arranged by size: 5024 1509 62 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2712 4403 276 Size: 53 83 186 936 1178 1691 2468 Fragments arranged by size: 2468 1691 1178 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SwaI