(Linear) (Six-base) MAPSORT of: KIEE5730.seq Check: 886 from: 1 to: 1890 KIEE5730.orf With 182 enzymes: SgfI * December 5, 2014 13:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 958 1523 1890 Size: 958 565 367 Fragments arranged by size: 958 565 367 AatII G_ACGT'C Cuts at: 0 709 1890 Size: 709 1181 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1465 1890 Size: 1465 425 AfeI AGC'GCT Cuts at: 0 793 1890 Size: 793 1097 AflIII A'CryG_T Cuts at: 0 248 1341 1890 Size: 248 1093 549 Fragments arranged by size: 1093 549 248 AgeI A'CCGG_T Cuts at: 0 1598 1890 Size: 1598 292 AleI CACnn'nnGTG Cuts at: 0 514 1890 Size: 514 1376 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 553 1573 1834 1890 Size: 553 1020 261 56 Fragments arranged by size: 1020 553 261 56 AlwNI CAG_nnn'CTG Cuts at: 0 72 1426 1890 Size: 72 1354 464 Fragments arranged by size: 1354 464 72 ApaI G_GGCC'C Cuts at: 0 159 1890 Size: 159 1731 ApaLI G'TGCA_C Cuts at: 0 1002 1890 Size: 1002 888 ApoI r'AATT_y Cuts at: 0 1115 1890 Size: 1115 775 AseI AT'TA_AT Cuts at: 0 602 1890 Size: 602 1288 AvaI C'yCGr_G Cuts at: 0 1538 1561 1890 Size: 1538 23 329 Fragments arranged by size: 1538 329 23 BanI G'GyrC_C Cuts at: 0 325 946 1296 1623 1681 1890 Size: 325 621 350 327 58 209 Fragments arranged by size: 621 350 327 325 209 58 BanII G_rGCy'C Cuts at: 0 159 473 839 1076 1890 Size: 159 314 366 237 814 Fragments arranged by size: 814 366 314 237 159 BbsI GAAGACnn'nnnn_ Cuts at: 0 217 1296 1890 Size: 217 1079 594 Fragments arranged by size: 1079 594 217 BbvCI CC'TCA_GC Cuts at: 0 340 1890 Size: 340 1550 BglI GCCn_nnn'nGGC Cuts at: 0 944 1138 1890 Size: 944 194 752 Fragments arranged by size: 944 752 194 BlpI GC'TnA_GC Cuts at: 0 838 1890 Size: 838 1052 Bme1580I G_kGCm'C Cuts at: 0 56 159 858 1006 1301 1464 1628 1890 Size: 56 103 699 148 295 163 164 262 Fragments arranged by size: 699 295 262 164 163 148 103 56 BmgBI CAC'GTC Cuts at: 0 555 1890 Size: 555 1335 BmrI ACTGGGnnnn_n' Cuts at: 0 231 1890 Size: 231 1659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 217 270 844 1246 1352 1890 Size: 217 53 574 402 106 538 Fragments arranged by size: 574 538 402 217 106 53 Bpu10I CC'TnA_GC Cuts at: 0 340 548 1890 Size: 340 208 1342 Fragments arranged by size: 1342 340 208 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1003 1890 Size: 1003 887 BsaI GGTCTCn'nnnn_ Cuts at: 0 169 1699 1796 1890 Size: 169 1530 97 94 Fragments arranged by size: 1530 169 97 94 BsaAI yAC'GTr Cuts at: 0 80 1890 Size: 80 1810 BsaHI Gr'CG_yC Cuts at: 0 706 1890 Size: 706 1184 BsaWI w'CCGG_w Cuts at: 0 1598 1890 Size: 1598 292 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 385 415 848 878 1117 1147 1890 Size: 385 30 433 30 239 30 743 Fragments arranged by size: 743 433 385 239 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 40 127 468 732 1125 1890 Size: 40 87 341 264 393 765 Fragments arranged by size: 765 393 341 264 87 40 BseYI C'CCAG_C Cuts at: 0 16 1697 1870 1890 Size: 16 1681 173 20 Fragments arranged by size: 1681 173 20 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 1018 1890 Size: 163 855 872 Fragments arranged by size: 872 855 163 BsiHKAI G_wGCw'C Cuts at: 0 554 839 1006 1076 1890 Size: 554 285 167 70 814 Fragments arranged by size: 814 554 285 167 70 BsmBI CGTCTCn'nnnn_ Cuts at: 0 619 935 1890 Size: 619 316 955 Fragments arranged by size: 955 619 316 Bsp1286I G_dGCh'C Cuts at: 0 56 159 473 554 839 858 1006 1076 Size: 56 103 314 81 285 19 148 70 Cuts at: 1076 1301 1464 1628 1890 Size: 225 163 164 262 Fragments arranged by size: 314 285 262 225 164 163 148 103 81 70 56 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 92 958 1523 1890 Size: 92 866 565 367 Fragments arranged by size: 866 565 367 92 BsrBI CCG'CTC Cuts at: 0 201 1890 Size: 201 1689 BsrFI r'CCGG_y Cuts at: 0 485 1598 1890 Size: 485 1113 292 Fragments arranged by size: 1113 485 292 BssSI C'ACGA_G Cuts at: 0 748 1863 1890 Size: 748 1115 27 Fragments arranged by size: 1115 748 27 BstAPI GCAn_nnn'nTGC Cuts at: 0 93 1890 Size: 93 1797 BstYI r'GATC_y Cuts at: 0 627 1890 Size: 627 1263 BtgI C'CryG_G Cuts at: 0 1634 1890 Size: 1634 256 BtsI GCAGTG_nn' Cuts at: 0 27 94 1006 1522 1890 Size: 27 67 912 516 368 Fragments arranged by size: 912 516 368 67 27 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1757 1792 1890 Size: 1757 35 98 Fragments arranged by size: 1757 98 35 DraIII CAC_nnn'GTG Cuts at: 0 105 1890 Size: 105 1785 DrdI GACnn_nn'nnGTC Cuts at: 0 1180 1890 Size: 1180 710 EaeI y'GGCC_r Cuts at: 0 74 495 1361 1484 1499 1890 Size: 74 421 866 123 15 391 Fragments arranged by size: 866 421 391 123 74 15 EarI CTCTTCn'nnn_ Cuts at: 0 121 478 1890 Size: 121 357 1412 Fragments arranged by size: 1412 357 121 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 598 890 1312 1890 Size: 598 292 422 578 Fragments arranged by size: 598 578 422 292 EcoICRI GAG'CTC Cuts at: 0 837 1074 1890 Size: 837 237 816 Fragments arranged by size: 837 816 237 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 217 270 844 1246 1352 1465 1890 Size: 217 53 574 402 106 113 425 Fragments arranged by size: 574 425 402 217 113 106 53 EcoNI CCTnn'n_nnAGG Cuts at: 0 1275 1890 Size: 1275 615 EcoO109I rG'GnC_Cy Cuts at: 0 155 156 384 1890 Size: 155 1 228 1506 Fragments arranged by size: 1506 228 155 1 HaeII r_GCGC'y Cuts at: 0 795 1890 Size: 795 1095 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 291 323 385 417 1300 1332 1890 Size: 291 32 62 32 883 32 558 Fragments arranged by size: 883 558 291 62 32 32 32 HincII GTy'rAC Cuts at: 0 282 1890 Size: 282 1608 HindIII A'AGCT_T Cuts at: 0 1432 1890 Size: 1432 458 MfeI C'AATT_G Cuts at: 0 692 1890 Size: 692 1198 MluI A'CGCG_T Cuts at: 0 1341 1890 Size: 1341 549 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 692 1890 Size: 692 1198 MscI TGG'CCA Cuts at: 0 76 1486 1501 1890 Size: 76 1410 15 389 Fragments arranged by size: 1410 389 76 15 MslI CAynn'nnrTG Cuts at: 0 514 1252 1480 1639 1795 1890 Size: 514 738 228 159 156 95 Fragments arranged by size: 738 514 228 159 156 95 MspA1I CmG'CkG Cuts at: 0 60 339 432 1266 1739 1890 Size: 60 279 93 834 473 151 Fragments arranged by size: 834 473 279 151 93 60 NspI r_CATG'y Cuts at: 0 96 252 1890 Size: 96 156 1638 Fragments arranged by size: 1638 156 96 PciI A'CATG_T Cuts at: 0 248 1890 Size: 248 1642 PflMI CCAn_nnn'nTGG Cuts at: 0 905 982 1518 1890 Size: 905 77 536 372 Fragments arranged by size: 905 536 372 77 PfoI T'CCnGG_A Cuts at: 0 194 1331 1890 Size: 194 1137 559 Fragments arranged by size: 1137 559 194 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 103 135 1890 Size: 103 32 1755 Fragments arranged by size: 1755 103 32 PspOMI G'GGCC_C Cuts at: 0 155 1890 Size: 155 1735 PstI C_TGCA'G Cuts at: 0 65 89 185 926 1813 1890 Size: 65 24 96 741 887 77 Fragments arranged by size: 887 741 96 77 65 24 PvuII CAG'CTG Cuts at: 0 339 432 1266 1739 1890 Size: 339 93 834 473 151 Fragments arranged by size: 834 473 339 151 93 SacI G_AGCT'C Cuts at: 0 839 1076 1890 Size: 839 237 814 Fragments arranged by size: 839 814 237 SapI GCTCTTCn'nnn_ Cuts at: 0 121 478 1890 Size: 121 357 1412 Fragments arranged by size: 1412 357 121 ScaI AGT'ACT Cuts at: 0 235 1890 Size: 235 1655 SfcI C'TryA_G Cuts at: 0 61 85 181 882 922 1275 1809 1890 Size: 61 24 96 701 40 353 534 81 Fragments arranged by size: 701 534 353 96 81 61 40 24 SmaI CCC'GGG Cuts at: 0 1540 1890 Size: 1540 350 SmlI C'TyrA_G Cuts at: 0 1018 1890 Size: 1018 872 SphI G_CATG'C Cuts at: 0 96 1890 Size: 96 1794 SrfI GCCC'GGGC Cuts at: 0 1540 1890 Size: 1540 350 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 213 862 1890 Size: 213 649 1028 Fragments arranged by size: 1028 649 213 TatI w'GTAC_w Cuts at: 0 233 369 1890 Size: 233 136 1521 Fragments arranged by size: 1521 233 136 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 848 880 1117 1149 1890 Size: 848 32 237 32 741 Fragments arranged by size: 848 741 237 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1063 1890 Size: 1063 827 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1494 1890 Size: 1494 396 XmaI C'CCGG_G Cuts at: 0 1538 1890 Size: 1538 352 XmnI GAAnn'nnTTC Cuts at: 0 1148 1210 1890 Size: 1148 62 680 Fragments arranged by size: 1148 680 62 ZraI GAC'GTC Cuts at: 0 707 1890 Size: 707 1183 Enzymes that do cut: AarI AatII AcuI AfeI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI BanI BanII BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstYI BtgI BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII MfeI MluI MmeI MscI MslI MspA1I NspI PciI PflMI PfoI PpiI PspOMI PstI PvuII SacI SapI ScaI SfcI SmaI SmlI SphI SrfI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AhdI AloI AscI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII BmtI BplI BsaBI BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI EagI EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI KpnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SspI StuI StyI SwaI XbaI XhoI