################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI AlwI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BbvCI BcgI BciVI BclI BfrI BglII BlnI BpmI BsaBI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I MamI MfeI MflI MluI MroI MseI MunI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SapI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspI StuI SwaI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AflIII 578 ACACGT A^C[GA][CT]GT BbrPI 939 CACGTG CAC^GTG BbsI 358 GAAGAC GAAGAC(2/6) BpuAI 358 GAAGAC GAAGAC(2/6) BsaAI 939 CACGTG [CT]AC^GT[GA] BsaI 1152 GGTCTC GGTCTC(1/5) BseRI 616 GAGGAG GAGGAG(10/8) BsgI 806 CTGCAC GTGCAG(16/14) BsiEI 438 CGACCG CG[GA][CT]^CG Bsp1407I 146 TGTACA T^GTACA BsrGI 146 TGTACA T^GTACA BssSI 1110 CTCGTG CACGAG(-5/-1) DraIII 1321 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG HincII 1189 GTCGAC GT[CT]^[GA]AC HindII 1189 GTCGAC GT[CT]^[GA]AC KspI 8 CCGCGG CCGC^GG NheI 741 GCTAGC G^CTAGC PleI 219 GACTC GAGTC(4/5) PmaCI 939 CACGTG CAC^GTG PmlI 939 CACGTG CAC^GTG SacII 8 CCGCGG CCGC^GG SalI 1189 GTCGAC G^TCGAC SbfI 549 CCTGCAGG CCTGCA^GG SmaI 29 CCCGGG CCC^GGG SmlI 420 CTTGAG C^T[CT][GA]AG Sse8387I 549 CCTGCAGG CCTGCA^GG SspBI 146 TGTACA T^GTACA TfiI 4 GAATC G^A[AT]TC XmaCI 29 CCCGGG C^CCGGG XmaI 29 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Alw44I 635 GTGCAC G^TGCAC 1327 GTGCAC AlwNI 553 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1066 CAGTGGCTG ApaLI 635 GTGCAC G^TGCAC 1327 GTGCAC AspHI 635 GTGCAC G[AT]GC[AT]^C 1327 GTGCAC BanI 95 GGCGCC G^G[CT][GA]CC 1102 GGCGCC BbeI 95 GGCGCC GGCGC^C 1102 GGCGCC BsiHKAI 635 GTGCAC G[AT]GC[AT]^C 1327 GTGCAC BsmAI 1153 GTCTC GTCTC(1/5) 1240 GTCTC BsmFI 1365 GGGAC GGGAC(10/14) 297 GTCCC BsrBI 1131 CCGCTC CCGCTC(-3/-3) 423 GAGCGG BsrDI 870 CATTGC GCAATG(2/0) 933 CATTGC DsaI 8 CCGCGG C^C[GA][CT]GG 875 CCGTGG EcoT14I 389 CCAAGG C^C[AT][AT]GG 1356 CCTTGG HinfI 4 GAATC G^A[ACGT]TC 219 GACTC HphI 893 TCACC GGTGA(8/7) 1031 TCACC KasI 95 GGCGCC G^GCGCC 1102 GGCGCC MaeIII 129 GTTAC ^GT[ACGT]AC 902 GTAAC MboII 358 GAAGA GAAGA(8/7) 366 GAAGA NarI 95 GGCGCC GG^CGCC 1102 GGCGCC NspI 140 ACATGC [GA]CATG^[CT] 188 ACATGC PvuII 305 CAGCTG CAG^CTG 403 CAGCTG SfoI 95 GGCGCC GGC^GCC 1102 GGCGCC StyI 389 CCAAGG C^C[AT][AT]GG 1356 CCTTGG XcmI 821 CCAACCTGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1057 CCACACCTGCAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI ApaI AvaI BalI BanII BbvI BcnI BfaI BglI BlpI BmyI Bpu1102I BsaHI BsaJI BsiYI BslI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MaeI MaeII MboI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI TspRI TthHB8I