(Circular) (Six-base) MAPSORT of: pFN21AE6496.seq Check: 9217 from: 1 to: 6301 pFN21AE6496 6301 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3075 3075 Size: 6301 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4111 278 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3030 3195 3264 3644 1937 Size: 1093 165 69 380 4594 Fragments arranged by size: 4594 1093 380 165 69 Acc65I G'GTAC_C Cuts at: 3622 3622 Size: 6301 AclI AA'CG_TT Cuts at: 1563 4428 4801 1563 Size: 2865 373 3063 Fragments arranged by size: 3063 2865 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4364 5748 6016 1632 Size: 2732 1384 268 1917 Fragments arranged by size: 2732 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6104 AflIII A'CryG_T Cuts at: 1203 2583 5200 6177 1203 Size: 1380 2617 977 1327 Fragments arranged by size: 2617 1380 1327 977 AgeI A'CCGG_T Cuts at: 5132 5132 Size: 6301 AhdI GACnn_n'nnGTC Cuts at: 5030 5030 Size: 6301 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6301 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2747 1184 Size: 1563 4738 AlwNI CAG_nnn'CTG Cuts at: 2563 3076 4044 5616 2563 Size: 513 968 1572 3248 Fragments arranged by size: 3248 1572 968 513 ApaI G_GGCC'C Cuts at: 2216 2436 3312 2216 Size: 220 876 5205 Fragments arranged by size: 5205 876 220 ApaLI G'TGCA_C Cuts at: 2640 3332 4358 5514 2640 Size: 692 1026 1156 3427 Fragments arranged by size: 3427 1156 1026 692 ApoI r'AATT_y Cuts at: 1493 3610 3836 3988 6097 1493 Size: 2117 226 152 2109 1697 Fragments arranged by size: 2117 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4855 160 Size: 872 3823 1606 Fragments arranged by size: 3823 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 2034 2228 2820 3305 3626 1870 Size: 73 91 194 592 485 321 4545 Fragments arranged by size: 4545 592 485 321 194 91 73 BamHI G'GATC_C Cuts at: 3631 3998 3631 Size: 367 5934 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2100 3107 3622 5077 618 Size: 325 217 627 313 1007 515 1455 1842 Fragments arranged by size: 1842 1455 1007 627 515 325 313 217 BanII G_rGCy'C Cuts at: 1391 2088 2216 2244 2330 2436 2570 2821 3312 Size: 697 128 28 86 106 134 251 491 Cuts at: 3312 3620 1391 Size: 308 4072 Fragments arranged by size: 4072 697 491 308 251 134 128 106 86 28 BbeI G_GCGC'C Cuts at: 2104 3111 2104 Size: 1007 5294 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2370 928 Size: 971 471 4859 Fragments arranged by size: 4859 971 471 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4492 4526 1903 Size: 34 2555 34 3678 Fragments arranged by size: 3678 2555 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4193 5403 4193 Size: 1210 5091 BclI T'GATC_A Cuts at: 1548 1548 Size: 6301 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 2092 2285 Size: 107 122 71 631 605 367 53 193 Cuts at: 2285 2559 2885 3528 3549 4912 136 Size: 274 326 643 21 1363 1525 Fragments arranged by size: 1525 1363 643 631 605 367 326 274 193 122 107 71 53 21 BglII A'GATC_T Cuts at: 6296 6296 Size: 6301 BlpI GC'TnA_GC Cuts at: 2329 2569 3356 3718 2329 Size: 240 787 362 4912 Fragments arranged by size: 4912 787 362 240 Bme1580I G_kGCm'C Cuts at: 1790 2169 2178 2216 2436 2644 2736 2843 3129 Size: 379 9 38 220 208 92 107 286 Cuts at: 3129 3312 3336 4362 5518 1790 Size: 183 24 1026 1156 2573 Fragments arranged by size: 2573 1156 1026 379 286 220 208 183 107 92 38 24 9 BmgBI CAC'GTC Cuts at: 1318 2368 2461 2586 3235 1318 Size: 1050 93 125 649 4384 Fragments arranged by size: 4384 1050 649 125 93 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2654 4032 4990 Size: 416 53 83 399 24 1237 1378 958 Cuts at: 4990 442 Size: 1753 Fragments arranged by size: 1753 1378 1237 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2750 1056 Size: 1694 4607 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4961 1239 Size: 3722 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2446 4426 5291 5589 2446 Size: 1980 865 298 3158 Fragments arranged by size: 3158 1980 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3163 4964 882 Size: 617 1664 1801 2219 Fragments arranged by size: 2219 1801 1664 617 BsaAI yAC'GTr Cuts at: 493 1204 2946 4092 493 Size: 711 1742 1146 2702 Fragments arranged by size: 2702 1742 1146 711 BsaBI GATnn'nnATC Cuts at: 6295 6295 Size: 6301 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2101 2300 2484 3027 Size: 53 83 186 936 568 199 184 543 Cuts at: 3027 3108 4108 4490 5125 275 Size: 81 1000 382 635 1451 Fragments arranged by size: 1451 1000 936 635 568 543 382 199 186 184 83 81 53 BsaWI w'CCGG_w Cuts at: 4734 5132 5406 5553 5964 4734 Size: 398 274 147 411 5071 Fragments arranged by size: 5071 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2209 2239 1178 Size: 30 165 30 806 30 5240 Fragments arranged by size: 5240 806 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2636 1186 Size: 1450 4851 BseYI C'CCAG_C Cuts at: 3022 3340 5504 6000 3022 Size: 318 2164 496 3323 Fragments arranged by size: 3323 2164 496 318 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2796 1163 Size: 573 1060 4668 Fragments arranged by size: 4668 1060 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2446 3751 4512 5540 1540 Size: 275 186 445 1305 761 1028 2301 Fragments arranged by size: 2301 1305 1028 761 445 275 186 BsiHKAI G_wGCw'C Cuts at: 2644 3336 4362 4447 5518 2644 Size: 692 1026 85 1071 3427 Fragments arranged by size: 3427 1071 1026 692 85 BsmI GAATG_Cn' Cuts at: 3812 3905 3812 Size: 93 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2169 2178 2216 2244 2330 2436 Size: 399 298 81 9 38 28 86 106 Cuts at: 2436 2570 2644 2736 2821 2843 3129 3312 3336 Size: 134 74 92 85 22 286 183 24 Cuts at: 3336 3620 4362 4447 5518 1391 Size: 284 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 298 286 284 183 134 106 92 86 85 85 81 74 38 28 24 22 9 BspEI T'CCGG_A Cuts at: 5964 5964 Size: 6301 BspHI T'CATG_A Cuts at: 4190 5920 4190 Size: 1730 4571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2029 2838 3075 3390 3656 844 Size: 1185 809 237 315 266 3489 Fragments arranged by size: 3489 1185 809 315 266 237 BsrBI CCG'CTC Cuts at: 1394 1823 2430 3138 4188 1394 Size: 429 607 708 1050 3507 Fragments arranged by size: 3507 1050 708 607 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2874 2937 4796 4970 65 Size: 1183 1626 63 1859 174 1396 Fragments arranged by size: 1859 1626 1396 1183 174 63 BsrFI r'CCGG_y Cuts at: 1700 1810 2720 2888 3001 4945 5132 1700 Size: 110 910 168 113 1944 187 2869 Fragments arranged by size: 2869 1944 910 187 168 113 110 BsrGI T'GTAC_A Cuts at: 96 2151 96 Size: 2055 4246 BssHII G'CGCG_C Cuts at: 1925 2546 2892 3053 3084 1925 Size: 621 346 161 31 5142 Fragments arranged by size: 5142 621 346 161 31 BssSI C'ACGA_G Cuts at: 3115 4361 5373 3115 Size: 1246 1012 4043 Fragments arranged by size: 4043 1246 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6301 BstBI TT'CG_AA Cuts at: 5105 5105 Size: 6301 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6301 BstYI r'GATC_y Cuts at: 1972 3631 3998 4383 4400 5852 5938 5950 6296 Size: 1659 367 385 17 1452 86 12 346 Cuts at: 6296 1972 Size: 1977 Fragments arranged by size: 1977 1659 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 2880 513 Size: 552 939 9 867 3934 Fragments arranged by size: 3934 939 867 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2170 3256 3465 487 Size: 683 56 944 1086 209 3323 Fragments arranged by size: 3323 1086 944 683 209 56 BtsI GCAGTG_nn' Cuts at: 2793 3077 3203 3817 4611 4631 2793 Size: 284 126 614 794 20 4463 Fragments arranged by size: 4463 794 614 284 126 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6266 DraI TTT'AAA Cuts at: 3605 3605 Size: 6301 DraIII CAC_nnn'GTG Cuts at: 3331 3331 Size: 6301 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5308 809 Size: 507 3992 1802 Fragments arranged by size: 3992 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2265 2286 Size: 54 1425 50 275 18 213 222 21 Cuts at: 2286 2318 2790 2886 3375 3520 3748 4637 6266 Size: 32 472 96 489 145 228 889 1629 Cuts at: 6266 8 Size: 43 Fragments arranged by size: 1629 1425 889 489 472 275 228 222 213 145 96 54 50 43 32 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3748 1537 Size: 275 1936 4090 Fragments arranged by size: 4090 1936 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4231 1353 Size: 2878 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4888 5262 5408 387 Size: 1064 138 3299 374 146 1280 Fragments arranged by size: 3299 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4364 4961 5748 6016 1239 Size: 393 2732 597 787 268 1524 Fragments arranged by size: 2732 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2212 2213 2433 2560 2212 Size: 1 220 127 5953 Fragments arranged by size: 5953 220 127 1 EcoRI G'AATT_C Cuts at: 3610 3988 3610 Size: 378 5923 FspI TGC'GCA Cuts at: 4807 4807 Size: 6301 HaeII r_GCGC'y Cuts at: 1678 2078 2104 2132 2280 2657 3111 5448 1678 Size: 400 26 28 148 377 454 2337 2531 Fragments arranged by size: 2531 2337 454 400 377 148 28 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4942 4974 5016 5048 1373 Size: 32 135 32 3370 32 42 32 2626 Fragments arranged by size: 3370 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3196 3645 3891 669 Size: 988 281 1258 449 246 3079 Fragments arranged by size: 3079 1258 988 449 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6301 HpaI GTT'AAC Cuts at: 3891 3891 Size: 6301 KasI G'GCGC_C Cuts at: 2100 3107 2100 Size: 1007 5294 KpnI G_GTAC'C Cuts at: 3626 3626 Size: 6301 MfeI C'AATT_G Cuts at: 3900 3900 Size: 6301 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2037 5415 5599 1305 Size: 732 3378 184 2007 Fragments arranged by size: 3378 2007 732 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2320 2792 3377 3522 10 Size: 54 1425 343 488 472 585 145 2789 Fragments arranged by size: 2789 1425 585 488 472 343 145 54 MslI CAynn'nnrTG Cuts at: 518 1166 2597 2879 2948 4259 4618 4777 518 Size: 648 1431 282 69 1311 359 159 2042 Fragments arranged by size: 2042 1431 1311 648 359 282 159 69 MspA1I CmG'CkG Cuts at: 1606 2015 2254 2312 2324 2410 2467 3717 4394 Size: 409 239 58 12 86 57 1250 677 Cuts at: 4394 5542 5787 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1250 1148 677 409 245 239 86 58 57 12 NaeI GCC'GGC Cuts at: 2722 2890 3003 2722 Size: 168 113 6020 Fragments arranged by size: 6020 168 113 NarI GG'CG_CC Cuts at: 2101 3108 2101 Size: 1007 5294 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4810 Fragments arranged by size: 4810 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6301 NgoMIV G'CCGG_C Cuts at: 2720 2888 3001 2720 Size: 168 113 6020 Fragments arranged by size: 6020 168 113 NheI G'CTAG_C Cuts at: 1052 2746 1052 Size: 1694 4607 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NspI r_CATG'y Cuts at: 1132 1229 2149 2197 3659 5204 1132 Size: 97 920 48 1462 1545 2229 Fragments arranged by size: 2229 1545 1462 920 97 48 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6301 PciI A'CATG_T Cuts at: 5200 5200 Size: 6301 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6301 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PmlI CAC'GTG Cuts at: 2946 2946 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4370 4402 5883 5915 4370 Size: 32 1481 32 4756 Fragments arranged by size: 4756 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6301 PsiI TTA'TAA Cuts at: 3871 3871 Size: 6301 PspOMI G'GGCC_C Cuts at: 2212 2432 3308 2212 Size: 220 876 5205 Fragments arranged by size: 5205 876 220 PstI C_TGCA'G Cuts at: 830 2082 2409 2559 3072 3653 830 Size: 1252 327 150 513 581 3478 Fragments arranged by size: 3478 1252 581 513 327 150 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6301 PvuII CAG'CTG Cuts at: 2312 2410 2312 Size: 98 6203 SacII CC_GC'GG Cuts at: 2016 2016 Size: 6301 SalI G'TCGA_C Cuts at: 1936 3194 3643 1936 Size: 1258 449 4594 Fragments arranged by size: 4594 1258 449 SbfI CC_TGCA'GG Cuts at: 2559 3653 2559 Size: 1094 5207 ScaI AGT'ACT Cuts at: 4549 6261 4549 Size: 1712 4589 SfcI C'TryA_G Cuts at: 826 1045 2078 2405 2499 2555 3068 3649 4784 Size: 219 1033 327 94 56 513 581 1135 Cuts at: 4784 5465 5656 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1033 681 581 513 327 219 191 94 56 SfoI GGC'GCC Cuts at: 2102 3109 2102 Size: 1007 5294 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 2036 3628 1872 Size: 164 1592 4545 Fragments arranged by size: 4545 1592 164 SmlI C'TyrA_G Cuts at: 820 1017 1943 2425 4405 5306 5568 820 Size: 197 926 482 1980 901 262 1553 Fragments arranged by size: 1980 1553 926 901 482 262 197 SnaBI TAC'GTA Cuts at: 493 4092 493 Size: 3599 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 3659 1132 Size: 2527 3774 SrfI GCCC'GGGC Cuts at: 2036 2036 Size: 6301 SspI AAT'ATT Cuts at: 5 52 4225 5 Size: 47 4173 2081 Fragments arranged by size: 4173 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6301 StyI C'CwwG_G Cuts at: 513 1065 2004 2394 3361 3740 513 Size: 552 939 390 967 379 3074 Fragments arranged by size: 3074 967 939 552 390 379 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4328 4345 4498 4683 5128 325 Size: 1255 2748 17 153 185 445 1498 Fragments arranged by size: 2748 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2151 4547 6259 Size: 274 80 33 51 1443 174 2396 1712 Cuts at: 6259 96 Size: 138 Fragments arranged by size: 2396 1712 1443 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2209 2241 2667 2699 6261 6293 2209 Size: 32 426 32 3562 32 2217 Fragments arranged by size: 3562 2217 426 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6301 XbaI T'CTAG_A Cuts at: 3637 3637 Size: 6301 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2833 3069 2833 Size: 236 6065 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6301 XmaI C'CCGG_G Cuts at: 1870 2034 3626 1870 Size: 164 1592 4545 Fragments arranged by size: 4545 1592 164 XmnI GAAnn'nnTTC Cuts at: 4430 4430 Size: 6301 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4109 276 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SwaI