(Linear) (Six-base) MAPSORT of: KIEE6496.seq Check: 881 from: 1 to: 1596 KIEE6496.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1070 1596 Size: 1070 526 AccI GT'mk_AC Cuts at: 0 1025 1190 1259 1596 Size: 1025 165 69 337 Fragments arranged by size: 1025 337 165 69 AflIII A'CryG_T Cuts at: 0 578 1596 Size: 578 1018 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 742 1596 Size: 742 854 AlwNI CAG_nnn'CTG Cuts at: 0 558 1071 1596 Size: 558 513 525 Fragments arranged by size: 558 525 513 ApaI G_GGCC'C Cuts at: 0 211 431 1307 1596 Size: 211 220 876 289 Fragments arranged by size: 876 289 220 211 ApaLI G'TGCA_C Cuts at: 0 635 1327 1596 Size: 635 692 269 Fragments arranged by size: 692 635 269 AvaI C'yCGr_G Cuts at: 0 29 223 815 1300 1596 Size: 29 194 592 485 296 Fragments arranged by size: 592 485 296 194 29 BanI G'GyrC_C Cuts at: 0 95 1102 1596 Size: 95 1007 494 Fragments arranged by size: 1007 494 95 BanII G_rGCy'C Cuts at: 0 83 211 239 325 431 565 816 1307 Size: 83 128 28 86 106 134 251 491 Cuts at: 1307 1596 Size: 289 Fragments arranged by size: 491 289 251 134 128 106 86 83 28 BbeI G_GCGC'C Cuts at: 0 99 1106 1596 Size: 99 1007 490 Fragments arranged by size: 1007 490 99 BbsI GAAGACnn'nnnn_ Cuts at: 0 365 1596 Size: 365 1231 BglI GCCn_nnn'nGGC Cuts at: 0 34 87 280 554 880 1523 1544 1596 Size: 34 53 193 274 326 643 21 52 Fragments arranged by size: 643 326 274 193 53 52 34 21 BlpI GC'TnA_GC Cuts at: 0 324 564 1351 1596 Size: 324 240 787 245 Fragments arranged by size: 787 324 245 240 Bme1580I G_kGCm'C Cuts at: 0 164 173 211 431 639 731 838 1124 Size: 164 9 38 220 208 92 107 286 Cuts at: 1124 1307 1331 1596 Size: 183 24 265 Fragments arranged by size: 286 265 220 208 183 164 107 92 38 24 9 BmgBI CAC'GTC Cuts at: 0 363 456 581 1230 1596 Size: 363 93 125 649 366 Fragments arranged by size: 649 366 363 125 93 BmrI ACTGGGnnnn_n' Cuts at: 0 649 1596 Size: 649 947 BmtI G_CTAG'C Cuts at: 0 745 1596 Size: 745 851 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 441 1596 Size: 441 1155 BsaI GGTCTCn'nnnn_ Cuts at: 0 1158 1596 Size: 1158 438 BsaAI yAC'GTr Cuts at: 0 941 1596 Size: 941 655 BsaHI Gr'CG_yC Cuts at: 0 96 295 479 1022 1103 1596 Size: 96 199 184 543 81 493 Fragments arranged by size: 543 493 199 184 96 81 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 204 234 1596 Size: 204 30 1362 Fragments arranged by size: 1362 204 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 631 1596 Size: 631 965 BseYI C'CCAG_C Cuts at: 0 1017 1335 1596 Size: 1017 318 261 Fragments arranged by size: 1017 318 261 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 791 1596 Size: 791 805 BsiEI CG_ry'CG Cuts at: 0 441 1596 Size: 441 1155 BsiHKAI G_wGCw'C Cuts at: 0 639 1331 1596 Size: 639 692 265 Fragments arranged by size: 692 639 265 Bsp1286I G_dGCh'C Cuts at: 0 83 164 173 211 239 325 431 565 Size: 83 81 9 38 28 86 106 134 Cuts at: 565 639 731 816 838 1124 1307 1331 1596 Size: 74 92 85 22 286 183 24 265 Fragments arranged by size: 286 265 183 134 106 92 86 85 83 81 74 38 28 24 22 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 24 833 1070 1385 1596 Size: 24 809 237 315 211 Fragments arranged by size: 809 315 237 211 24 BsrBI CCG'CTC Cuts at: 0 425 1133 1596 Size: 425 708 463 Fragments arranged by size: 708 463 425 BsrDI GCAATG_nn' Cuts at: 0 869 932 1596 Size: 869 63 664 Fragments arranged by size: 869 664 63 BsrFI r'CCGG_y Cuts at: 0 715 883 996 1596 Size: 715 168 113 600 Fragments arranged by size: 715 600 168 113 BsrGI T'GTAC_A Cuts at: 0 146 1596 Size: 146 1450 BssHII G'CGCG_C Cuts at: 0 541 887 1048 1079 1596 Size: 541 346 161 31 517 Fragments arranged by size: 541 517 346 161 31 BssSI C'ACGA_G Cuts at: 0 1110 1596 Size: 1110 486 BtgI C'CryG_G Cuts at: 0 8 875 1596 Size: 8 867 721 Fragments arranged by size: 867 721 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 165 1251 1460 1596 Size: 165 1086 209 136 Fragments arranged by size: 1086 209 165 136 BtsI GCAGTG_nn' Cuts at: 0 788 1072 1198 1596 Size: 788 284 126 398 Fragments arranged by size: 788 398 284 126 DraIII CAC_nnn'GTG Cuts at: 0 1326 1596 Size: 1326 270 EaeI y'GGCC_r Cuts at: 0 38 260 281 313 785 881 1370 1515 Size: 38 222 21 32 472 96 489 145 Cuts at: 1515 1596 Size: 81 Fragments arranged by size: 489 472 222 145 96 81 38 32 21 EcoO109I rG'GnC_Cy Cuts at: 0 207 208 428 555 1596 Size: 207 1 220 127 1041 Fragments arranged by size: 1041 220 207 127 1 HaeII r_GCGC'y Cuts at: 0 73 99 127 275 652 1106 1596 Size: 73 26 28 148 377 454 490 Fragments arranged by size: 490 454 377 148 73 28 26 HincII GTy'rAC Cuts at: 0 1191 1596 Size: 1191 405 KasI G'GCGC_C Cuts at: 0 95 1102 1596 Size: 95 1007 494 Fragments arranged by size: 1007 494 95 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 32 1596 Size: 32 1564 MscI TGG'CCA Cuts at: 0 315 787 1372 1517 1596 Size: 315 472 585 145 79 Fragments arranged by size: 585 472 315 145 79 MslI CAynn'nnrTG Cuts at: 0 592 874 943 1596 Size: 592 282 69 653 Fragments arranged by size: 653 592 282 69 MspA1I CmG'CkG Cuts at: 0 10 249 307 319 405 462 1596 Size: 10 239 58 12 86 57 1134 Fragments arranged by size: 1134 239 86 58 57 12 10 NaeI GCC'GGC Cuts at: 0 717 885 998 1596 Size: 717 168 113 598 Fragments arranged by size: 717 598 168 113 NarI GG'CG_CC Cuts at: 0 96 1103 1596 Size: 96 1007 493 Fragments arranged by size: 1007 493 96 NgoMIV G'CCGG_C Cuts at: 0 715 883 996 1596 Size: 715 168 113 600 Fragments arranged by size: 715 600 168 113 NheI G'CTAG_C Cuts at: 0 741 1596 Size: 741 855 NspI r_CATG'y Cuts at: 0 144 192 1596 Size: 144 48 1404 Fragments arranged by size: 1404 144 48 PmlI CAC'GTG Cuts at: 0 941 1596 Size: 941 655 PspOMI G'GGCC_C Cuts at: 0 207 427 1303 1596 Size: 207 220 876 293 Fragments arranged by size: 876 293 220 207 PstI C_TGCA'G Cuts at: 0 77 404 554 1067 1596 Size: 77 327 150 513 529 Fragments arranged by size: 529 513 327 150 77 PvuII CAG'CTG Cuts at: 0 307 405 1596 Size: 307 98 1191 Fragments arranged by size: 1191 307 98 SacII CC_GC'GG Cuts at: 0 11 1596 Size: 11 1585 SalI G'TCGA_C Cuts at: 0 1189 1596 Size: 1189 407 SbfI CC_TGCA'GG Cuts at: 0 554 1596 Size: 554 1042 SfcI C'TryA_G Cuts at: 0 73 400 494 550 1063 1596 Size: 73 327 94 56 513 533 Fragments arranged by size: 533 513 327 94 73 56 SfoI GGC'GCC Cuts at: 0 97 1104 1596 Size: 97 1007 492 Fragments arranged by size: 1007 492 97 SmaI CCC'GGG Cuts at: 0 31 1596 Size: 31 1565 SmlI C'TyrA_G Cuts at: 0 420 1596 Size: 420 1176 SrfI GCCC'GGGC Cuts at: 0 31 1596 Size: 31 1565 StyI C'CwwG_G Cuts at: 0 389 1356 1596 Size: 389 967 240 Fragments arranged by size: 967 389 240 TatI w'GTAC_w Cuts at: 0 146 1596 Size: 146 1450 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 204 236 662 694 1596 Size: 204 32 426 32 902 Fragments arranged by size: 902 426 204 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 828 1064 1596 Size: 828 236 532 Fragments arranged by size: 828 532 236 XmaI C'CCGG_G Cuts at: 0 29 1596 Size: 29 1567 Enzymes that do cut: AarI AccI AflIII AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BtgI BtgZI BtsI DraIII EaeI EcoO109I HaeII HincII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI PmlI PspOMI PstI PvuII SacII SalI SbfI SfcI SfoI SmaI SmlI SrfI StyI TatI TstI XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BplI BpmI Bpu10I BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI NcoI NdeI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI TaqII Tth111I XbaI XhoI XmnI ZraI