################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AgeI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaI BamHI BbeI BcgI BciVI BfrI BglI BglII BsaBI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI DraI DraIII DsaI EagI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PvuI SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AcyI 1104 GACGCC G[GA]^CG[CT]C AflIII 287 ACACGT A^C[GA][CT]GT AhdI 452 GACTCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 1031 GGATC GGATC(4/5) Aor51HI 635 AGCGCT AGC^GCT AspEI 452 GACTCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 200 TGCGCA TGC^GCA AvrII 418 CCTAGG C^CTAGG BalI 915 TGGCCA TGG^CCA BbsI 766 GTCTTC GAAGAC(2/6) BbvCI 813 GCTGAGG CCTCAGC(-5/-2) BclI 824 TGATCA T^GATCA BcnI 962 CCGGG CC^[GC]GG BfaI 419 CTAG C^TAG BlnI 418 CCTAGG C^CTAGG BlpI 442 GCTGAGC GC^T[ACGT]AGC Bpu1102I 442 GCTGAGC GC^T[ACGT]AGC BpuAI 766 GTCTTC GAAGAC(2/6) BsaHI 1104 GACGCC G[GA]^CG[CT]C BsaWI 644 TCCGGT [AT]^CCGG[AT] BseRI 901 GAGGAG GAGGAG(10/8) Bsp1407I 275 TGTACA T^GTACA BspHI 489 TCATGA T^CATGA BsrDI 1035 CATTGC GCAATG(2/0) BsrGI 275 TGTACA T^GTACA BsrI 120 ACTGG ACTGG(1/-1) BssSI 898 CACGAG CACGAG(-5/-1) CelII 442 GCTGAGC GC^T[ACGT]AGC CpoI 646 CGGTCCG CG^G[AT]CCG DraII 221 GGGTCCC [GA]G^G[ACGT]CC[CT] DrdI 721 GACATCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 915 TGGCCA [CT]^GGCC[GA] Eam1105I 452 GACTCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 400 CTCTTC CTCTTC(1/4) Eco47III 635 AGCGCT AGC^GCT EcoNI 1209 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 221 GGGTCCC [GA]G^G[ACGT]CC[CT] FbaI 824 TGATCA T^GATCA FspI 200 TGCGCA TGC^GCA Hin1I 1104 GACGCC G[GA]^CG[CT]C Ksp632I 400 CTCTTC CTCTTC(1/4) MaeI 419 CTAG C^TAG MluNI 915 TGGCCA TGG^CCA MscI 915 TGGCCA TGG^CCA MseI 209 TTAA T^TAA MspA1I 1026 CAGCTG C[AC]G^C[GT]G NciI 962 CCGGG CC^[GC]GG PpuMI 221 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 86 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 73 CTGCAG CTGCA^G PvuII 1026 CAGCTG CAG^CTG RcaI 489 TCATGA T^CATGA RsrII 646 CGGTCCG CG^G[AT]CCG SacI 445 GAGCTC GAGCT^C SapI 399 GCTCTTC GCTCTTC(1/4) SspBI 275 TGTACA T^GTACA StuI 1169 AGGCCT AGG^CCT Tru9I 209 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 300 GTCGAC GT^[AC][GT]AC 933 GTCGAC AlwNI 972 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG 1129 CAGACCCTG BanI 126 GGTGCC G^G[CT][GA]CC 1230 GGTGCC BanII 445 GAGCTC G[GA]GC[CT]^C 1194 GAGCCC BbrPI 475 CACGTG CAC^GTG 919 CACGTG BsaAI 475 CACGTG [CT]AC^GT[GA] 919 CACGTG BsgI 1126 GTGCAG GTGCAG(16/14) 1281 GTGCAG BspMI 749 ACCTGC ACCTGC(4/8) 883 GCAGGT BstAPI 390 GCACATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1166 GCAAGGCCTGC BstXI 780 CCACAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1154 CCACAAAGGTGG FauI 928 GCGGG CCCGC(4/6) 996 GCGGG HapII 645 CCGG C^CGG 962 CCGG HgaI 1104 GACGC GACGC(5/10) 313 GCGTC HincII 300 GTCGAC GT[CT]^[GA]AC 933 GTCGAC HindII 300 GTCGAC GT[CT]^[GA]AC 933 GTCGAC HinfI 452 GACTC G^A[ACGT]TC 652 GACTC HpaII 645 CCGG C^CGG 962 CCGG HphI 886 GGTGA GGTGA(8/7) 434 TCACC MspI 645 CCGG C^CGG 962 CCGG PleI 452 GACTC GAGTC(4/5) 652 GACTC PmaCI 475 CACGTG CAC^GTG 919 CACGTG PmlI 475 CACGTG CAC^GTG 919 CACGTG SalI 300 GTCGAC G^TCGAC 933 GTCGAC XcmI 70 CCACTGCAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 563 CCAAGACCTTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BbvI BmyI BpmI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HhaI HinP1I ItaI MaeII MaeIII MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I