Restriction map for
pFN21AE4182
##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
BalI BamHI BanI BbeI BbvCI
BciVI BclI BlpI Bpu1102I BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstEII BstPI BstUI Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MluI MluNI MroI MscI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmeI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AflII 949 CTTAAG C^TTAAG
AlwNI 1522 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 181 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1637 CTCGGG C^[CT]CG[GA]G
AvrII 1090 CCTAGG C^CTAGG
BbrPI 1754 CACGTG CAC^GTG
BbsI 396 GAAGAC GAAGAC(2/6)
BcgI 1435 GCACCAATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 949 CTTAAG C^TTAAG
BglI 1711 GCCTCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1609 AGATCT A^GATCT
BlnI 1090 CCTAGG C^CTAGG
BpuAI 396 GAAGAC GAAGAC(2/6)
BsaAI 1754 CACGTG [CT]AC^GT[GA]
BsaBI 920 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 45 GGGAC GGGAC(10/14)
BsoBI 1637 CTCGGG C^[CT]CG[GA]G
BspLU11I 112 ACATGT A^CATGT
BspMI 840 ACCTGC ACCTGC(4/8)
BssSI 2137 CACGAG CACGAG(-5/-1)
Bst1107I 430 GTATAC GTA^TAC
BstBI 170 TTCGAA TT^CGAA
BstXI 1878 CCATGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1609 AGATCT [GA]^GATC[CT]
BstZ17I 430 GTATAC GTA^TAC
DraII 809 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 1718 TGGCCG [CT]^GGCC[GA]
EcoO109I 809 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1642 GATATC GAT^ATC
EcoT22I 854 ATGCAT ATGCA^T
HaeIII 1719 GGCC GG^CC
HincII 197 GTCGAC GT[CT]^[GA]AC
HindII 197 GTCGAC GT[CT]^[GA]AC
HindIII 2167 AAGCTT A^AGCTT
MamI 920 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 448 CAATTG C^AATTG
MflI 1609 AGATCT [GA]^GATC[CT]
MspA1I 1239 CAGCGG C[AC]G^C[GT]G
MunI 448 CAATTG C^AATTG
NsiI 854 ATGCAT ATGCA^T
NspV 170 TTCGAA TT^CGAA
PmaCI 1754 CACGTG CAC^GTG
PmlI 1754 CACGTG CAC^GTG
PpuMI 809 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1409 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 85 GAGCTC GAGCT^C
SalI 197 GTCGAC G^TCGAC
SapI 87 GCTCTTC GCTCTTC(1/4)
ScaI 362 AGTACT AGT^ACT
SfuI 170 TTCGAA TT^CGAA
XhoII 1609 AGATCT [GA]^GATC[CT]
XmnI 181 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 112 ACATGT A^C[GA][CT]GT
1753 ACACGT
AlwI 340 GATCC GGATC(4/5)
1078 GGATC
BanII 85 GAGCTC G[GA]GC[CT]^C
1194 GGGCTC
BcnI 1383 CCGGG CC^[GC]GG
1721 CCGGG
BsmI 852 GAATGC GAATGC(1/-1)
2041 GAATGC
BstNI 875 CCAGG CC^[AT]GG
1047 CCAGG
DsaI 80 CCATGG C^C[GA][CT]GG
1878 CCATGG
EcoRII 875 CCAGG ^CC[AT]GG
1047 CCAGG
HapII 1383 CCGG C^CGG
1721 CCGG
HpaII 1383 CCGG C^CGG
1721 CCGG
MaeII 1001 ACGT A^CGT
1755 ACGT
MspI 1383 CCGG C^CGG
1721 CCGG
MvaI 875 CCAGG CC^[AT]GG
1047 CCAGG
NciI 1383 CCGGG CC^[GC]GG
1721 CCGGG
NcoI 80 CCATGG C^CATGG
1878 CCATGG
PstI 405 CTGCAG CTGCA^G
464 CTGCAG
SfaNI 1320 GCATC GCATC(5/9)
2068 GCATC
SmlI 949 CTTAAG C^T[CT][GA]AG
1324 CTCAAG
Tsp45I 1416 GTCAC ^GT[GC]AC
1524 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI AspHI AvaII BbvI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I Cac8I Cfr13I
DdeI DpnII EarI EcoT14I Fnu4HI
FokI HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfcI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I