(Circular) (Six-base) MAPSORT of: pFN21AE4182.seq Check: 6464 from: 1 to: 6886 pFN21AE4182 6886 bp With 182 enzymes: SgfI * March 28, 2012 13:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2854 2854 Size: 6886 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4696 278 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2203 2436 3800 3878 4229 1937 Size: 266 233 1364 78 351 4594 Fragments arranged by size: 4594 1364 351 266 233 78 Acc65I G'GTAC_C Cuts at: 4207 4207 Size: 6886 AclI AA'CG_TT Cuts at: 1563 5013 5386 1563 Size: 3450 373 3063 Fragments arranged by size: 3450 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2705 2980 3141 3357 3761 3959 4949 6333 Size: 1073 275 161 216 404 198 990 1384 Cuts at: 6333 6601 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1073 990 404 275 268 216 198 161 AflII C'TTAA_G Cuts at: 820 1017 2954 820 Size: 197 1937 4752 Fragments arranged by size: 4752 1937 197 AflIII A'CryG_T Cuts at: 1203 2117 3758 5785 6762 1203 Size: 914 1641 2027 977 1327 Fragments arranged by size: 2027 1641 1327 977 914 AgeI A'CCGG_T Cuts at: 5717 5717 Size: 6886 AhdI GACnn_n'nnGTC Cuts at: 5615 5615 Size: 6886 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6886 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6886 AlwNI CAG_nnn'CTG Cuts at: 3532 4629 6201 3532 Size: 1097 1572 4217 Fragments arranged by size: 4217 1572 1097 ApaLI G'TGCA_C Cuts at: 4943 6099 4943 Size: 1156 5730 ApoI r'AATT_y Cuts at: 1493 2172 2458 2653 2889 2948 4195 4421 4573 Size: 679 286 195 236 59 1247 226 152 Cuts at: 4573 6682 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1247 679 286 236 226 195 152 59 AseI AT'TA_AT Cuts at: 160 1032 5440 160 Size: 872 4408 1606 Fragments arranged by size: 4408 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 AvaI C'yCGr_G Cuts at: 1870 1943 3642 4211 1870 Size: 73 1699 569 4545 Fragments arranged by size: 4545 1699 569 73 AvrII C'CTAG_G Cuts at: 3095 3095 Size: 6886 BamHI G'GATC_C Cuts at: 4216 4583 4216 Size: 367 6519 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4207 5662 618 Size: 325 217 627 2420 1455 1842 Fragments arranged by size: 2420 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2094 3203 4205 1391 Size: 703 1109 1002 4072 Fragments arranged by size: 4072 1109 1002 703 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2408 928 Size: 971 509 5406 Fragments arranged by size: 5406 971 509 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3429 3463 5077 5111 1903 Size: 34 1492 34 1614 34 3678 Fragments arranged by size: 3678 1614 1492 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4778 5988 4778 Size: 1210 5676 BclI T'GATC_A Cuts at: 1548 1548 Size: 6886 BfrBI ATG'CAT Cuts at: 2861 2861 Size: 6886 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3722 5497 136 Size: 107 122 71 631 605 2050 1775 1525 Fragments arranged by size: 2050 1775 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3614 6881 3614 Size: 3267 3619 BlpI GC'TnA_GC Cuts at: 4303 4303 Size: 6886 Bme1580I G_kGCm'C Cuts at: 1790 4947 6103 1790 Size: 3157 1156 2573 Fragments arranged by size: 3157 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6886 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3801 3844 4617 Size: 416 53 83 399 24 2384 43 773 Cuts at: 4617 5575 442 Size: 958 1753 Fragments arranged by size: 2384 1753 958 773 416 399 83 53 43 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6886 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2422 2422 Size: 6886 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2863 3296 3300 3794 5546 1239 Size: 1624 433 4 494 1752 2579 Fragments arranged by size: 2579 1752 1624 494 433 4 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3314 5011 5876 6174 3314 Size: 1697 865 298 4026 Fragments arranged by size: 4026 1697 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5549 882 Size: 617 4050 2219 Fragments arranged by size: 4050 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3761 4677 493 Size: 711 2557 916 2702 Fragments arranged by size: 2702 2557 916 711 BsaBI GATnn'nnATC Cuts at: 2929 6880 2929 Size: 3951 2935 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4693 5075 5710 275 Size: 53 83 186 936 3160 382 635 1451 Fragments arranged by size: 3160 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5319 5717 5991 6138 6549 5319 Size: 398 274 147 411 5656 Fragments arranged by size: 5656 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6661 Fragments arranged by size: 6661 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2754 3009 4144 1186 Size: 1568 255 1135 3928 Fragments arranged by size: 3928 1568 1135 255 BseYI C'CCAG_C Cuts at: 2970 6089 6585 2970 Size: 3119 496 3271 Fragments arranged by size: 3271 3119 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2772 3423 4143 1163 Size: 573 1036 651 720 3906 Fragments arranged by size: 3906 1036 720 651 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4336 5097 6125 1540 Size: 275 186 2335 761 1028 2301 Fragments arranged by size: 2335 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2094 3734 3758 4947 5032 6103 2094 Size: 1640 24 1189 85 1071 2877 Fragments arranged by size: 2877 1640 1189 1071 85 24 BsmI GAATG_Cn' Cuts at: 2863 4052 4397 4490 2863 Size: 1189 345 93 5259 Fragments arranged by size: 5259 1189 345 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 3203 3734 3758 4205 4947 5032 Size: 399 304 1109 531 24 447 742 85 Cuts at: 5032 6103 1391 Size: 1071 2174 Fragments arranged by size: 2174 1109 1071 742 531 447 399 304 85 24 BspEI T'CCGG_A Cuts at: 6549 6549 Size: 6886 BspHI T'CATG_A Cuts at: 4775 6505 4775 Size: 1730 5156 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2854 4241 844 Size: 2010 1387 3489 Fragments arranged by size: 3489 2010 1387 BsrBI CCG'CTC Cuts at: 1394 1823 4773 1394 Size: 429 2950 3507 Fragments arranged by size: 3507 2950 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5381 5555 65 Size: 1183 4133 174 1396 Fragments arranged by size: 4133 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5530 5717 1700 Size: 110 3720 187 2869 Fragments arranged by size: 3720 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6886 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6886 BssSI C'ACGA_G Cuts at: 4142 4946 5958 4142 Size: 804 1012 5070 Fragments arranged by size: 5070 1012 804 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6886 BstBI TT'CG_AA Cuts at: 2176 5690 2176 Size: 3514 3372 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6886 BstXI CCAn_nnnn'nTGG Cuts at: 3890 3890 Size: 6886 BstYI r'GATC_y Cuts at: 1972 3614 4216 4583 4968 4985 6437 6523 6535 Size: 1642 602 367 385 17 1452 86 12 Cuts at: 6535 6881 1972 Size: 346 1977 Fragments arranged by size: 1977 1642 1452 602 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2437 2437 Size: 6886 BtgI C'CryG_G Cuts at: 513 1065 2004 2085 3883 513 Size: 552 939 81 1798 3516 Fragments arranged by size: 3516 1798 939 552 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6147 Fragments arranged by size: 6147 683 56 BtsI GCAGTG_nn' Cuts at: 2784 4402 5196 5216 2784 Size: 1618 794 20 4454 Fragments arranged by size: 4454 1618 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6886 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4137 4172 529 Size: 35 3573 35 3243 Fragments arranged by size: 3573 3243 35 35 DraI TTT'AAA Cuts at: 4190 4190 Size: 6886 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5893 809 Size: 507 4577 1802 Fragments arranged by size: 4577 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3723 4333 5222 Size: 54 1425 50 275 18 1893 610 889 Cuts at: 5222 6851 8 Size: 1629 43 Fragments arranged by size: 1893 1629 1425 889 610 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4333 1537 Size: 275 2521 4090 Fragments arranged by size: 4090 2521 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2099 2323 2518 3432 3978 4816 1353 Size: 746 224 195 914 546 838 3423 Fragments arranged by size: 3423 914 838 746 546 224 195 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2325 2856 5473 5847 5993 387 Size: 1064 138 736 531 2617 374 146 1280 Fragments arranged by size: 2617 1280 1064 736 531 374 146 138 EcoICRI GAG'CTC Cuts at: 2092 2092 Size: 6886 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2705 2863 2980 3141 3296 3300 3357 Size: 393 1073 158 117 161 155 4 57 Cuts at: 3357 3761 3794 3959 4949 5546 6333 6601 1239 Size: 404 33 165 990 597 787 268 1524 Fragments arranged by size: 1524 1073 990 787 597 404 393 268 165 161 158 155 117 57 33 4 EcoO109I rG'GnC_Cy Cuts at: 2815 2815 Size: 6886 EcoRI G'AATT_C Cuts at: 4195 4573 4195 Size: 378 6508 EcoRV GAT'ATC Cuts at: 3649 3649 Size: 6886 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3989 3989 Size: 6886 FspI TGC'GCA Cuts at: 5392 5392 Size: 6886 HaeII r_GCGC'y Cuts at: 1678 6033 1678 Size: 4355 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2376 2408 2731 2763 3467 Size: 32 135 32 804 32 323 32 704 Cuts at: 3467 3499 5527 5559 5601 5633 1373 Size: 32 2028 32 42 32 2626 Fragments arranged by size: 2626 2028 804 704 323 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2204 4230 4476 669 Size: 988 281 266 2026 246 3079 Fragments arranged by size: 3079 2026 988 281 266 246 HindIII A'AGCT_T Cuts at: 748 4172 748 Size: 3424 3462 HpaI GTT'AAC Cuts at: 4476 4476 Size: 6886 KpnI G_GTAC'C Cuts at: 4211 4211 Size: 6886 MfeI C'AATT_G Cuts at: 2453 4485 2453 Size: 2032 4854 MluI A'CGCG_T Cuts at: 6762 6762 Size: 6886 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3871 6000 6184 1305 Size: 2566 2129 184 2007 Fragments arranged by size: 2566 2129 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5064 Fragments arranged by size: 5064 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2076 2538 3656 4083 4844 5203 5362 Size: 648 910 462 1118 427 761 359 159 Cuts at: 5362 518 Size: 2042 Fragments arranged by size: 2042 1118 910 761 648 462 427 359 159 MspA1I CmG'CkG Cuts at: 1606 3246 4302 4979 6127 6372 1606 Size: 1640 1056 677 1148 245 2120 Fragments arranged by size: 2120 1640 1148 1056 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2085 3883 513 Size: 552 939 81 1798 3516 Fragments arranged by size: 3516 1798 939 552 81 NdeI CA'TA_TG Cuts at: 387 387 Size: 6886 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6886 NotI GC'GGCC_GC Cuts at: 4333 4333 Size: 6886 NsiI A_TGCA'T Cuts at: 2863 2863 Size: 6886 NspI r_CATG'y Cuts at: 1132 1229 2121 2543 3338 4244 5789 1132 Size: 97 892 422 795 906 1545 2229 Fragments arranged by size: 2229 1545 906 892 795 422 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6886 PciI A'CATG_T Cuts at: 2117 5785 2117 Size: 3668 3218 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6886 PfoI T'CCnGG_A Cuts at: 2879 2879 Size: 6886 PmeI GTTT'AAAC Cuts at: 4190 4190 Size: 6886 PmlI CAC'GTG Cuts at: 3761 3761 Size: 6886 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2563 2595 4955 4987 6468 6500 2563 Size: 32 2360 32 1481 32 2949 Fragments arranged by size: 2949 2360 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2815 2815 Size: 6886 PshAI GACnn'nnGTC Cuts at: 1613 3418 1613 Size: 1805 5081 PsiI TTA'TAA Cuts at: 4456 4456 Size: 6886 PstI C_TGCA'G Cuts at: 830 2414 2473 4238 830 Size: 1584 59 1765 3478 Fragments arranged by size: 3478 1765 1584 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6886 SacI G_AGCT'C Cuts at: 2094 2094 Size: 6886 SalI G'TCGA_C Cuts at: 1936 2202 4228 1936 Size: 266 2026 4594 Fragments arranged by size: 4594 2026 266 SapI GCTCTTCn'nnn_ Cuts at: 2099 2099 Size: 6886 SbfI CC_TGCA'GG Cuts at: 4238 4238 Size: 6886 ScaI AGT'ACT Cuts at: 2369 5134 6846 2369 Size: 2765 1712 2409 Fragments arranged by size: 2765 2409 1712 SfcI C'TryA_G Cuts at: 826 1045 2062 2337 2410 2469 2757 3509 4234 Size: 219 1017 275 73 59 288 752 725 Cuts at: 4234 5369 6050 6241 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1017 752 725 681 288 275 219 191 73 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6886 SmaI CCC'GGG Cuts at: 1872 4213 1872 Size: 2341 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2954 3329 4990 5891 6153 820 Size: 197 926 1011 375 1661 901 262 1553 Fragments arranged by size: 1661 1553 1011 926 901 375 262 197 SnaBI TAC'GTA Cuts at: 493 4677 493 Size: 4184 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6886 SphI G_CATG'C Cuts at: 1132 4244 1132 Size: 3112 3774 SspI AAT'ATT Cuts at: 5 52 4810 5 Size: 47 4758 2081 Fragments arranged by size: 4758 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6886 StyI C'CwwG_G Cuts at: 513 1065 2004 2085 3095 3883 4325 513 Size: 552 939 81 1010 788 442 3074 Fragments arranged by size: 3074 1010 939 788 552 442 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2766 4913 4930 5083 5268 5713 325 Size: 1255 1186 2147 17 153 185 445 1498 Fragments arranged by size: 2147 1498 1255 1186 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2367 3938 5132 Size: 274 80 33 51 1443 390 1571 1194 Cuts at: 5132 6844 96 Size: 1712 138 Fragments arranged by size: 1712 1571 1443 1194 390 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6846 6878 6846 Size: 32 6854 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6886 XbaI T'CTAG_A Cuts at: 4222 4222 Size: 6886 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6886 XmaI C'CCGG_G Cuts at: 1870 4211 1870 Size: 2341 4545 XmnI GAAnn'nnTTC Cuts at: 2190 5015 2190 Size: 2825 4061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4694 276 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI