(Linear) (Six-base) MAPSORT of: KIEE4182.seq Check: 8785 from: 1 to: 2181 KIEE4182.orf With 182 enzymes: SgfI * March 22, 2012 17:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 849 2181 Size: 849 1332 AccI GT'mk_AC Cuts at: 0 198 431 1795 1873 2181 Size: 198 233 1364 78 308 Fragments arranged by size: 1364 308 233 198 78 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 700 975 1136 1352 1756 1954 2181 Size: 700 275 161 216 404 198 227 Fragments arranged by size: 700 404 275 227 216 198 161 AflII C'TTAA_G Cuts at: 0 949 2181 Size: 949 1232 AflIII A'CryG_T Cuts at: 0 112 1753 2181 Size: 112 1641 428 Fragments arranged by size: 1641 428 112 AlwNI CAG_nnn'CTG Cuts at: 0 1527 2181 Size: 1527 654 ApoI r'AATT_y Cuts at: 0 167 453 648 884 943 2181 Size: 167 286 195 236 59 1238 Fragments arranged by size: 1238 286 236 195 167 59 AvaI C'yCGr_G Cuts at: 0 1637 2181 Size: 1637 544 AvrII C'CTAG_G Cuts at: 0 1090 2181 Size: 1090 1091 BanII G_rGCy'C Cuts at: 0 89 1198 2181 Size: 89 1109 983 Fragments arranged by size: 1109 983 89 BbsI GAAGACnn'nnnn_ Cuts at: 0 403 2181 Size: 403 1778 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1424 1458 2181 Size: 1424 34 723 Fragments arranged by size: 1424 723 34 BfrBI ATG'CAT Cuts at: 0 856 2181 Size: 856 1325 BglI GCCn_nnn'nGGC Cuts at: 0 1717 2181 Size: 1717 464 BglII A'GATC_T Cuts at: 0 1609 2181 Size: 1609 572 BmrI ACTGGGnnnn_n' Cuts at: 0 1796 1839 2181 Size: 1796 43 342 Fragments arranged by size: 1796 342 43 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 417 2181 Size: 417 1764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 858 1291 1295 1789 2181 Size: 858 433 4 494 392 Fragments arranged by size: 858 494 433 392 4 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1309 2181 Size: 1309 872 BsaAI yAC'GTr Cuts at: 0 1756 2181 Size: 1756 425 BsaBI GATnn'nnATC Cuts at: 0 924 2181 Size: 924 1257 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 749 1004 2139 2181 Size: 749 255 1135 42 Fragments arranged by size: 1135 749 255 42 BseYI C'CCAG_C Cuts at: 0 965 2181 Size: 965 1216 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 767 1418 2138 2181 Size: 767 651 720 43 Fragments arranged by size: 767 720 651 43 BsiHKAI G_wGCw'C Cuts at: 0 89 1729 1753 2181 Size: 89 1640 24 428 Fragments arranged by size: 1640 428 89 24 BsmI GAATG_Cn' Cuts at: 0 858 2047 2181 Size: 858 1189 134 Fragments arranged by size: 1189 858 134 Bsp1286I G_dGCh'C Cuts at: 0 89 1198 1729 1753 2181 Size: 89 1109 531 24 428 Fragments arranged by size: 1109 531 428 89 24 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 849 2181 Size: 849 1332 BssSI C'ACGA_G Cuts at: 0 2137 2181 Size: 2137 44 BstBI TT'CG_AA Cuts at: 0 171 2181 Size: 171 2010 BstXI CCAn_nnnn'nTGG Cuts at: 0 1885 2181 Size: 1885 296 BstYI r'GATC_y Cuts at: 0 1609 2181 Size: 1609 572 BstZ17I GTA'TAC Cuts at: 0 432 2181 Size: 432 1749 BtgI C'CryG_G Cuts at: 0 80 1878 2181 Size: 80 1798 303 Fragments arranged by size: 1798 303 80 BtsI GCAGTG_nn' Cuts at: 0 779 2181 Size: 779 1402 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2132 2167 2181 Size: 2132 35 14 Fragments arranged by size: 2132 35 14 EaeI y'GGCC_r Cuts at: 0 1718 2181 Size: 1718 463 EarI CTCTTCn'nnn_ Cuts at: 0 94 318 513 1427 1973 2181 Size: 94 224 195 914 546 208 Fragments arranged by size: 914 546 224 208 195 94 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 320 851 2181 Size: 320 531 1330 Fragments arranged by size: 1330 531 320 EcoICRI GAG'CTC Cuts at: 0 87 2181 Size: 87 2094 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 700 858 975 1136 1291 1295 1352 1756 Size: 700 158 117 161 155 4 57 404 Cuts at: 1756 1789 1954 2181 Size: 33 165 227 Fragments arranged by size: 700 404 227 165 161 158 155 117 57 33 4 EcoO109I rG'GnC_Cy Cuts at: 0 810 2181 Size: 810 1371 EcoRV GAT'ATC Cuts at: 0 1644 2181 Size: 1644 537 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1984 2181 Size: 1984 197 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 371 403 726 758 1462 1494 2181 Size: 371 32 323 32 704 32 687 Fragments arranged by size: 704 687 371 323 32 32 32 HincII GTy'rAC Cuts at: 0 199 2181 Size: 199 1982 HindIII A'AGCT_T Cuts at: 0 2167 2181 Size: 2167 14 MfeI C'AATT_G Cuts at: 0 448 2181 Size: 448 1733 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1866 2181 Size: 1866 315 MslI CAynn'nnrTG Cuts at: 0 71 533 1651 2078 2181 Size: 71 462 1118 427 103 Fragments arranged by size: 1118 462 427 103 71 MspA1I CmG'CkG Cuts at: 0 1241 2181 Size: 1241 940 NcoI C'CATG_G Cuts at: 0 80 1878 2181 Size: 80 1798 303 Fragments arranged by size: 1798 303 80 NsiI A_TGCA'T Cuts at: 0 858 2181 Size: 858 1323 NspI r_CATG'y Cuts at: 0 116 538 1333 2181 Size: 116 422 795 848 Fragments arranged by size: 848 795 422 116 PciI A'CATG_T Cuts at: 0 112 2181 Size: 112 2069 PfoI T'CCnGG_A Cuts at: 0 874 2181 Size: 874 1307 PmlI CAC'GTG Cuts at: 0 1756 2181 Size: 1756 425 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 558 590 2181 Size: 558 32 1591 Fragments arranged by size: 1591 558 32 PpuMI rG'GwC_Cy Cuts at: 0 810 2181 Size: 810 1371 PshAI GACnn'nnGTC Cuts at: 0 1413 2181 Size: 1413 768 PstI C_TGCA'G Cuts at: 0 409 468 2181 Size: 409 59 1713 Fragments arranged by size: 1713 409 59 SacI G_AGCT'C Cuts at: 0 89 2181 Size: 89 2092 SalI G'TCGA_C Cuts at: 0 197 2181 Size: 197 1984 SapI GCTCTTCn'nnn_ Cuts at: 0 94 2181 Size: 94 2087 ScaI AGT'ACT Cuts at: 0 364 2181 Size: 364 1817 SfcI C'TryA_G Cuts at: 0 57 332 405 464 752 1504 2181 Size: 57 275 73 59 288 752 677 Fragments arranged by size: 752 677 288 275 73 59 57 SmlI C'TyrA_G Cuts at: 0 949 1324 2181 Size: 949 375 857 Fragments arranged by size: 949 857 375 StyI C'CwwG_G Cuts at: 0 80 1090 1878 2181 Size: 80 1010 788 303 Fragments arranged by size: 1010 788 303 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 761 2181 Size: 761 1420 TatI w'GTAC_w Cuts at: 0 362 1933 2181 Size: 362 1571 248 Fragments arranged by size: 1571 362 248 XmnI GAAnn'nnTTC Cuts at: 0 185 2181 Size: 185 1996 Enzymes that do cut: AarI AccI AcuI AflII AflIII AlwNI ApoI AvaI AvrII BanII BbsI BcgI BfrBI BglI BglII BmrI BplI BpmI BpuEI BsaAI BsaBI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BssSI BstBI BstXI BstYI BstZ17I BtgI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I HincII HindIII MfeI MmeI MslI MspA1I NcoI NsiI NspI PciI PfoI PmlI PpiI PpuMI PshAI PstI SacI SalI SapI ScaI SfcI SmlI StyI TaqII TatI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI BaeI BamHI BanI BbeI BbvCI BciVI BclI BlpI Bme1580I BmgBI BmtI Bpu10I BsaI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EcoNI EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PmeI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI