################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AvaII BamHI BbeI BbrPI BbvCI BcgI BfrI BglI BlpI BpmI Bpu1102I BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BsrFI BssHII BssSI BstEII BstPI BstUI BstXI Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI HaeII HpaI HphI KasI KpnI KspI MluI MroI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AflIII 3731 ACATGT A^C[GA][CT]GT Alw44I 331 GTGCAC G^TGCAC ApaLI 331 GTGCAC G^TGCAC AseI 3625 ATTAAT AT^TAAT AsnI 3625 ATTAAT AT^TAAT AviII 1944 TGCGCA TGC^GCA BanI 217 GGTGCC G^G[CT][GA]CC BanII 2899 GAGCTC G[GA]GC[CT]^C BcnI 1738 CCGGG CC^[GC]GG BglII 1168 AGATCT A^GATCT BsaBI 223 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 3943 GAGGAG GAGGAG(10/8) Bsp1407I 3447 TGTACA T^GTACA BspLU11I 3731 ACATGT A^CATGT BsrDI 3504 CATTGC GCAATG(2/0) BsrGI 3447 TGTACA T^GTACA Bst1107I 1322 GTATAC GTA^TAC BstBI 880 TTCGAA TT^CGAA BstNI 3207 CCAGG CC^[AT]GG BstZ17I 1322 GTATAC GTA^TAC CfoI 1945 GCGC GCG^C Cfr13I 68 GGGCC G^G[ACGT]CC DsaI 312 CCATGG C^C[GA][CT]GG EcoRII 3207 CCAGG ^CC[AT]GG FauI 1024 CCCGC CCCGC(4/6) FspI 1944 TGCGCA TGC^GCA HapII 1738 CCGG C^CGG HgaI 40 GACGC GACGC(5/10) HhaI 1945 GCGC GCG^C HinP1I 1945 GCGC G^CGC HincII 517 GTTGAC GT[CT]^[GA]AC HindII 517 GTTGAC GT[CT]^[GA]AC HpaII 1738 CCGG C^CGG MamI 223 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3884 CAATTG C^AATTG MspA1I 713 CAGCTG C[AC]G^C[GT]G MspI 1738 CCGG C^CGG MunI 3884 CAATTG C^AATTG MvaI 3207 CCAGG CC^[AT]GG NciI 1738 CCGGG CC^[GC]GG NcoI 312 CCATGG C^CATGG NheI 343 GCTAGC G^CTAGC NspV 880 TTCGAA TT^CGAA PshBI 3625 ATTAAT AT^TAAT PvuII 713 CAGCTG CAG^CTG SacI 2899 GAGCTC GAGCT^C Sau96I 68 GGGCC G^G[ACGT]CC SfuI 880 TTCGAA TT^CGAA SmlI 3901 CTTGAG C^T[CT][GA]AG SphI 497 GCATGC GCATG^C SspBI 3447 TGTACA T^GTACA Tsp45I 3469 GTGAC ^GT[GC]AC XbaI 3786 TCTAGA T^CTAGA XcmI 3167 CCATGCTAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AatII 130 GACGTC GACGT^C 520 GACGTC AccI 109 GTAGAC GT^[AC][GT]AC 1322 GTATAC AlwI 1477 GATCC GGATC(4/5) 478 GGATC AvaI 2095 CCCGAG C^[CT]CG[GA]G 2452 CCCGAG BalI 81 TGGCCA TGG^CCA 952 TGGCCA BciVI 1887 GGATAC GTATCC(6/5) 2727 GGATAC BclI 1224 TGATCA T^GATCA 3459 TGATCA BsaAI 948 TACGTG [CT]AC^GT[GA] 1050 TACGTG BsaI 3689 GAGACC GGTCTC(1/5) 1988 GGTCTC BsmI 426 GAATGC GAATGC(1/-1) 3258 GAATGC BsoBI 2095 CCCGAG C^[CT]CG[GA]G 2452 CCCGAG BspHI 591 TCATGA T^CATGA 3111 TCATGA BspMI 1663 ACCTGC ACCTGC(4/8) 3492 ACCTGC BssKI 1738 CCGGG ^CC[ACGT]GG 3207 CCAGG BstAPI 3508 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3640 GCAGGAGATGC BstYI 1168 AGATCT [GA]^GATC[CT] 1476 AGATCC EaeI 81 TGGCCA [CT]^GGCC[GA] 952 TGGCCA FbaI 1224 TGATCA T^GATCA 3459 TGATCA HindIII 720 AAGCTT A^AGCTT 786 AAGCTT MflI 1168 AGATCT [GA]^GATC[CT] 1476 AGATCC MluNI 81 TGGCCA TGG^CCA 952 TGGCCA MscI 81 TGGCCA TGG^CCA 952 TGGCCA RcaI 591 TCATGA T^CATGA 3111 TCATGA SapI 1372 GAAGAGC GCTCTTC(1/4) 2998 GAAGAGC ScrFI 1738 CCGGG CC^[ACGT]GG 3207 CCAGG SspI 199 AATATT AAT^ATT 1268 AATATT XhoII 1168 AGATCT [GA]^GATC[CT] 1476 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI ApoI AspHI AvrII BbsI BbvI BfaI BlnI BmyI BpuAI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BstF5I Cac8I DdeI DpnII DraI EarI EcoT14I Fnu4HI FokI HaeIII Hin1I HinfI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MwoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I