(Circular) (Six-base) MAPSORT of: pFN21AE3457.seq Check: 814 from: 1 to: 8728 pFN21AE3457 8728 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2139 2529 6538 278 Size: 53 83 186 936 603 390 4009 2468 Fragments arranged by size: 4009 2468 936 603 390 186 83 53 AccI GT'mk_AC Cuts at: 1937 2115 3328 6071 1937 Size: 178 1213 2743 4594 Fragments arranged by size: 4594 2743 1213 178 Acc65I G'GTAC_C Cuts at: 6049 6049 Size: 8728 AclI AA'CG_TT Cuts at: 1563 6855 7228 1563 Size: 5292 373 3063 Fragments arranged by size: 5292 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2495 3680 4037 4394 4551 5258 5619 6791 Size: 863 1185 357 357 157 707 361 1172 Cuts at: 6791 8175 8443 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1185 1172 863 707 361 357 357 268 157 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8531 AflIII A'CryG_T Cuts at: 1203 5736 7627 8604 1203 Size: 4533 1891 977 1327 Fragments arranged by size: 4533 1891 1327 977 AgeI A'CCGG_T Cuts at: 7559 7559 Size: 8728 AhdI GACnn_n'nnGTC Cuts at: 7457 7457 Size: 8728 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8728 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8728 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5487 5519 5487 Size: 32 8696 AlwNI CAG_nnn'CTG Cuts at: 6471 8043 6471 Size: 1572 7156 ApaLI G'TGCA_C Cuts at: 2336 6785 7941 2336 Size: 4449 1156 3123 Fragments arranged by size: 4449 3123 1156 ApoI r'AATT_y Cuts at: 1493 2402 2850 2948 3050 3526 3883 4597 6037 Size: 909 448 98 102 476 357 714 1440 Cuts at: 6037 6263 6415 8524 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1440 909 714 476 448 357 226 152 102 98 AseI AT'TA_AT Cuts at: 160 1032 5631 7282 160 Size: 872 4599 1651 1606 Fragments arranged by size: 4599 1651 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8728 AvaI C'yCGr_G Cuts at: 1870 1943 4100 4457 6053 1870 Size: 73 2157 357 1596 4545 Fragments arranged by size: 4545 2157 1596 357 73 AvrII C'CTAG_G Cuts at: 2909 2975 3011 3077 2909 Size: 66 36 66 8560 Fragments arranged by size: 8560 66 66 36 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5723 5756 5723 Size: 33 8695 BamHI G'GATC_C Cuts at: 6058 6425 6058 Size: 367 8361 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2222 6049 7504 618 Size: 325 217 627 435 3827 1455 1842 Fragments arranged by size: 3827 1842 1455 627 435 325 217 BanII G_rGCy'C Cuts at: 1391 4908 6047 1391 Size: 3517 1139 4072 Fragments arranged by size: 4072 3517 1139 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2027 2977 3009 3079 3647 4004 928 Size: 971 128 950 32 70 568 357 5652 Fragments arranged by size: 5652 971 950 568 357 128 70 32 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6919 6953 1903 Size: 34 4982 34 3678 Fragments arranged by size: 4982 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3886 4726 6620 7830 3886 Size: 840 1894 1210 4784 Fragments arranged by size: 4784 1894 1210 840 BclI T'GATC_A Cuts at: 1548 3229 5464 1548 Size: 1681 2235 4812 Fragments arranged by size: 4812 2235 1681 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7339 136 Size: 107 122 71 631 605 5667 1525 Fragments arranged by size: 5667 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3173 8723 3173 Size: 5550 3178 BlpI GC'TnA_GC Cuts at: 6145 6145 Size: 8728 Bme1580I G_kGCm'C Cuts at: 1790 2340 6789 7945 1790 Size: 550 4449 1156 2573 Fragments arranged by size: 4449 2573 1156 550 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8728 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2080 6459 7417 Size: 416 53 83 399 24 663 4379 958 Cuts at: 7417 442 Size: 1753 Fragments arranged by size: 4379 1753 958 663 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2352 1056 Size: 1296 7432 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2079 2079 Size: 8728 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 7388 1239 Size: 6149 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5927 6853 7718 8016 5927 Size: 926 865 298 6639 Fragments arranged by size: 6639 926 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3999 5688 7391 882 Size: 617 2500 1689 1703 2219 Fragments arranged by size: 2500 2219 1703 1689 617 BsaAI yAC'GTr Cuts at: 493 1204 2955 3057 6519 493 Size: 711 1751 102 3462 2702 Fragments arranged by size: 3462 2702 1751 711 102 BsaBI GATnn'nnATC Cuts at: 2232 8722 2232 Size: 6490 2238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2046 2136 2526 6535 Size: 53 83 186 936 513 90 390 4009 Cuts at: 6535 6917 7552 275 Size: 382 635 1451 Fragments arranged by size: 4009 1451 936 635 513 390 382 186 90 83 53 BsaWI w'CCGG_w Cuts at: 7161 7559 7833 7980 8391 7161 Size: 398 274 147 411 7498 Fragments arranged by size: 7498 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5913 5943 1178 Size: 30 165 30 4510 30 3963 Fragments arranged by size: 4510 3963 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 5963 1186 Size: 4777 3951 BseYI C'CCAG_C Cuts at: 7931 8427 7931 Size: 496 8232 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2343 2673 2739 1163 Size: 573 607 330 66 7152 Fragments arranged by size: 7152 607 573 330 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6178 6939 7967 1540 Size: 275 186 4177 761 1028 2301 Fragments arranged by size: 4177 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2340 4908 5583 6789 6874 7945 2340 Size: 2568 675 1206 85 1071 3123 Fragments arranged by size: 3123 2568 1206 1071 675 85 BsmI GAATG_Cn' Cuts at: 2437 5269 6239 6332 2437 Size: 2832 970 93 4833 Fragments arranged by size: 4833 2832 970 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2340 4908 5583 6047 6789 6874 7945 Size: 399 550 2568 675 464 742 85 1071 Cuts at: 7945 1391 Size: 2174 Fragments arranged by size: 2568 2174 1071 742 675 550 464 399 85 BspEI T'CCGG_A Cuts at: 8391 8391 Size: 8728 BspHI T'CATG_A Cuts at: 2596 5116 6617 8347 2596 Size: 2520 1501 1730 2977 Fragments arranged by size: 2977 2520 1730 1501 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3677 5506 6083 844 Size: 2833 1829 577 3489 Fragments arranged by size: 3489 2833 1829 577 BsrBI CCG'CTC Cuts at: 1394 1823 6615 1394 Size: 429 4792 3507 Fragments arranged by size: 4792 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5508 7223 7397 65 Size: 1183 4260 1715 174 1396 Fragments arranged by size: 4260 1715 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7372 7559 1700 Size: 110 5562 187 2869 Fragments arranged by size: 5562 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5452 96 Size: 5356 3372 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8728 BssSI C'ACGA_G Cuts at: 6788 7800 6788 Size: 1012 7716 BstAPI GCAn_nnn'nTGC Cuts at: 794 5519 5651 794 Size: 4725 132 3871 Fragments arranged by size: 4725 3871 132 BstBI TT'CG_AA Cuts at: 2886 7532 2886 Size: 4646 4082 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8728 BstYI r'GATC_y Cuts at: 1972 3173 3481 6058 6425 6810 6827 8279 8365 Size: 1201 308 2577 367 385 17 1452 86 Cuts at: 8365 8377 8723 1972 Size: 12 346 1977 Fragments arranged by size: 2577 1977 1452 1201 385 367 346 308 86 17 12 BstZ17I GTA'TAC Cuts at: 3329 3329 Size: 8728 BtgI C'CryG_G Cuts at: 513 1065 2317 513 Size: 552 1252 6924 Fragments arranged by size: 6924 1252 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7989 Fragments arranged by size: 7989 683 56 BtsI GCAGTG_nn' Cuts at: 4918 6244 7038 7058 4918 Size: 1326 794 20 6588 Fragments arranged by size: 6588 1326 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8728 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8693 DraI TTT'AAA Cuts at: 4929 5253 5976 6032 4929 Size: 324 723 56 7625 Fragments arranged by size: 7625 723 324 56 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7735 809 Size: 507 6419 1802 Fragments arranged by size: 6419 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2086 2957 6175 Size: 54 1425 50 275 18 256 871 3218 Cuts at: 6175 7064 8693 8 Size: 889 1629 43 Fragments arranged by size: 3218 1629 1425 889 871 275 256 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6175 1537 Size: 275 4363 4090 Fragments arranged by size: 4363 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2048 3372 3627 4341 4935 4940 4998 5072 Size: 695 1324 255 714 594 5 58 74 Cuts at: 5072 6658 1353 Size: 1586 3423 Fragments arranged by size: 3423 1586 1324 714 695 594 255 74 58 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2714 7315 7689 7835 387 Size: 1064 138 1125 4601 374 146 1280 Fragments arranged by size: 4601 1280 1125 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4906 4906 Size: 8728 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2495 3680 4037 4394 4551 5258 5619 Size: 393 863 1185 357 357 157 707 361 Cuts at: 5619 6791 7388 8175 8443 1239 Size: 1172 597 787 268 1524 Fragments arranged by size: 1524 1185 1172 863 787 707 597 393 361 357 357 268 157 EcoRI G'AATT_C Cuts at: 6037 6415 6037 Size: 378 8350 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3671 4028 3671 Size: 357 8371 FspI TGC'GCA Cuts at: 3951 7234 3951 Size: 3283 5445 HaeII r_GCGC'y Cuts at: 1678 7875 1678 Size: 6197 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2158 2190 2276 2308 2807 Size: 32 135 32 586 32 86 32 499 Cuts at: 2807 2825 2839 2857 3175 3207 3466 3498 4094 Size: 18 14 18 318 32 259 32 596 Cuts at: 4094 4126 4451 4483 4537 4569 7369 7401 7443 Size: 32 325 32 54 32 2800 32 42 Cuts at: 7443 7475 1373 Size: 32 2626 Fragments arranged by size: 2800 2626 596 586 499 325 318 259 135 86 54 42 32 32 32 32 32 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 2524 6072 6318 669 Size: 988 281 586 3548 246 3079 Fragments arranged by size: 3548 3079 988 586 281 246 HindIII A'AGCT_T Cuts at: 748 2725 2791 748 Size: 1977 66 6685 Fragments arranged by size: 6685 1977 66 HpaI GTT'AAC Cuts at: 6318 6318 Size: 8728 KpnI G_GTAC'C Cuts at: 6053 6053 Size: 8728 MfeI C'AATT_G Cuts at: 5889 6327 5889 Size: 438 8290 MluI A'CGCG_T Cuts at: 8604 8604 Size: 8728 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3716 4073 4430 7842 8026 1305 Size: 2411 357 357 3412 184 2007 Fragments arranged by size: 3412 2411 2007 357 357 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2088 2959 10 Size: 54 1425 343 256 871 5779 Fragments arranged by size: 5779 1425 871 343 256 54 MslI CAynn'nnrTG Cuts at: 518 1166 2333 2595 3419 3776 4133 4490 4746 Size: 648 1167 262 824 357 357 357 256 Cuts at: 4746 5115 5121 6686 7045 7204 518 Size: 369 6 1565 359 159 2042 Fragments arranged by size: 2042 1565 1167 824 648 369 359 357 357 357 262 256 159 6 MspA1I CmG'CkG Cuts at: 1606 2720 6144 6821 7969 8214 1606 Size: 1114 3424 677 1148 245 2120 Fragments arranged by size: 3424 2120 1148 1114 677 245 NcoI C'CATG_G Cuts at: 513 1065 2317 513 Size: 552 1252 6924 Fragments arranged by size: 6924 1252 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8728 NheI G'CTAG_C Cuts at: 1052 2348 1052 Size: 1296 7432 NotI GC'GGCC_GC Cuts at: 6175 6175 Size: 8728 NspI r_CATG'y Cuts at: 1132 1229 2506 3192 3721 4078 4435 5155 5687 Size: 97 1277 686 529 357 357 720 532 Cuts at: 5687 5740 6086 7631 1132 Size: 53 346 1545 2229 Fragments arranged by size: 2229 1545 1277 720 686 532 529 357 357 346 97 53 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8728 PciI A'CATG_T Cuts at: 5736 7627 5736 Size: 1891 6837 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8728 PmeI GTTT'AAAC Cuts at: 6032 6032 Size: 8728 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4890 4922 5037 5069 6797 6829 8310 8342 4890 Size: 32 115 32 1728 32 1481 32 5276 Fragments arranged by size: 5276 1728 1481 115 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8728 PsiI TTA'TAA Cuts at: 6298 6298 Size: 8728 PstI C_TGCA'G Cuts at: 830 2539 2560 2671 4388 4913 6080 830 Size: 1709 21 111 1717 525 1167 3478 Fragments arranged by size: 3478 1717 1709 1167 525 111 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8728 PvuII CAG'CTG Cuts at: 2720 2720 Size: 8728 SacI G_AGCT'C Cuts at: 4908 4908 Size: 8728 SalI G'TCGA_C Cuts at: 1936 6070 1936 Size: 4134 4594 SapI GCTCTTCn'nnn_ Cuts at: 3372 4998 3372 Size: 1626 7102 SbfI CC_TGCA'GG Cuts at: 6080 6080 Size: 8728 ScaI AGT'ACT Cuts at: 6976 8688 6976 Size: 1712 7016 SfcI C'TryA_G Cuts at: 826 1045 2535 2556 2667 4384 4909 6076 7211 Size: 219 1490 21 111 1717 525 1167 1135 Cuts at: 7211 7892 8083 826 Size: 681 191 1471 Fragments arranged by size: 1717 1490 1471 1167 1135 681 525 219 191 111 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8728 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8728 SmaI CCC'GGG Cuts at: 1872 6055 1872 Size: 4183 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5906 6832 7733 7995 820 Size: 197 926 3963 926 901 262 1553 Fragments arranged by size: 3963 1553 926 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6519 493 Size: 6026 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8728 SphI G_CATG'C Cuts at: 1132 2506 6086 1132 Size: 1374 3580 3774 Fragments arranged by size: 3774 3580 1374 SspI AAT'ATT Cuts at: 5 52 2206 3275 6652 5 Size: 47 2154 1069 3377 2081 Fragments arranged by size: 3377 2154 2081 1069 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8728 StyI C'CwwG_G Cuts at: 513 1065 2317 2694 2760 2826 2909 2975 3011 Size: 552 1252 377 66 66 83 66 36 Cuts at: 3011 3077 3585 3804 3942 4161 4299 4518 4656 Size: 66 508 219 138 219 138 219 138 Cuts at: 4656 6167 513 Size: 1511 3074 Fragments arranged by size: 3074 1511 1252 552 508 377 219 219 219 138 138 138 83 66 66 66 66 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6755 6772 6925 7110 7555 325 Size: 1255 5175 17 153 185 445 1498 Fragments arranged by size: 5175 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4828 5452 6974 Size: 274 80 33 51 1443 2851 624 1522 Cuts at: 6974 8686 96 Size: 1712 138 Fragments arranged by size: 2851 1712 1522 1443 624 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8688 8720 8688 Size: 32 8696 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8728 XbaI T'CTAG_A Cuts at: 5791 6064 5791 Size: 273 8455 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5179 5179 Size: 8728 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8728 XmaI C'CCGG_G Cuts at: 1870 6053 1870 Size: 4183 4545 XmnI GAAnn'nnTTC Cuts at: 6857 6857 Size: 8728 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2137 2527 6536 276 Size: 53 83 186 936 603 390 4009 2468 Fragments arranged by size: 4009 2468 936 603 390 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI