Restriction map for
pFN21AB7201
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# NO CUT ENZYMES ( 101 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI AocI ApaI
ApoI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BsaBI BsaWI BseAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrDI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraI Eam1105I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SalI SapI SbfI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SspI StuI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 2002 GACGTC GACGT^C
AccI 1207 GTCTAC GT^[AC][GT]AC
AlwNI 1426 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 1027 GGCGCGCC GG^CGCGCC
BalI 1223 TGGCCA TGG^CCA
BbrPI 1153 CACGTG CAC^GTG
BlpI 1374 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1374 GCTCAGC GC^T[ACGT]AGC
BsaI 1165 GAGACC GGTCTC(1/5)
BseRI 1129 GAGGAG GAGGAG(10/8)
BsmBI 885 CGTCTC CGTCTC(1/5)
Bsp1407I 272 TGTACA T^GTACA
BsrGI 272 TGTACA T^GTACA
BssSI 1003 CACGAG CACGAG(-5/-1)
BstEII 10 GGTTACC G^GT[ACGT]ACC
BstF5I 1527 CATCC GGATG(2/0)
BstPI 10 GGTTACC G^GT[ACGT]ACC
BstXI 89 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 230 AGATCC [GA]^GATC[CT]
CelII 1374 GCTCAGC GC^T[ACGT]AGC
DdeI 1375 CTCAG C^T[ACGT]AG
DraIII 435 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 10 GGTTACC G^GT[ACGT]ACC
FokI 1527 CATCC GGATG(9/13)
HinfI 1831 GAGTC G^A[ACGT]TC
MflI 230 AGATCC [GA]^GATC[CT]
MluNI 1223 TGGCCA TGG^CCA
MscI 1223 TGGCCA TGG^CCA
NotI 76 GCGGCCGC GC^GGCCGC
PleI 1831 GAGTC GAGTC(4/5)
PmaCI 1153 CACGTG CAC^GTG
PmlI 1153 CACGTG CAC^GTG
SacI 2040 GAGCTC GAGCT^C
SfiI 78 GGCCGCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 272 TGTACA T^GTACA
TaqI 902 TCGA T^CGA
Tsp45I 25 GTGAC ^GT[GC]AC
TthHB8I 902 TCGA T^CGA
XhoII 230 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Aor51HI 59 AGCGCT AGC^GCT
911 AGCGCT
BsaAI 1153 CACGTG [CT]AC^GT[GA]
1414 TACGTG
BsgI 264 GTGCAG GTGCAG(16/14)
1017 GTGCAG
BsmAI 1165 GAGAC GTCTC(1/5)
886 GTCTC
BspMI 1439 ACCTGC ACCTGC(4/8)
1715 ACCTGC
Eco47III 59 AGCGCT AGC^GCT
911 AGCGCT
MaeIII 11 GTTAC ^GT[ACGT]AC
25 GTGAC
MslI 150 CACCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1359 CATCGCGGTG
PflMI 1232 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1425 CCAGAGCCTGG
PpuMI 1712 GGGACCT [GA]G^G[AT]CC[CT]
1950 GGGACCC
PstI 55 CTGCAG CTGCA^G
1717 CTGCAG
ScaI 1316 AGTACT AGT^ACT
1766 AGTACT
SmaI 688 CCCGGG CCC^GGG
1107 CCCGGG
SmlI 250 CTCAAG C^T[CT][GA]AG
1762 CTCAAG
SphI 100 GCATGC GCATG^C
395 GCATGC
Van91I 1232 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1425 CCAGAGCCTGG
XcmI 1358 CCATCGCGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1490 CCATGTTCCTGCTGG
XmaCI 688 CCCGGG C^CCGGG
1107 CCCGGG
XmaI 688 CCCGGG C^CCGGG
1107 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI ApaLI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaJI BsiEI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssHII
BssKI BstAPI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DpnII DraII
DrdI DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI StyI TseI TspRI