(Circular) (Six-base) MAPSORT of: pFN21AB7201.seq Check: 2463 from: 1 to: 6787 pFN21AB7201 6787 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4011 4597 278 Size: 53 83 186 936 2475 586 2468 Fragments arranged by size: 2475 2468 936 586 186 83 53 AccI GT'mk_AC Cuts at: 1937 3213 4130 1937 Size: 1276 917 4594 Fragments arranged by size: 4594 1276 917 Acc65I G'GTAC_C Cuts at: 4108 4108 Size: 6787 AclI AA'CG_TT Cuts at: 1563 4914 5287 1563 Size: 3351 373 3063 Fragments arranged by size: 3351 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4850 6234 6502 1632 Size: 3218 1384 268 1917 Fragments arranged by size: 3218 1917 1384 268 AfeI AGC'GCT Cuts at: 2066 2918 2066 Size: 852 5935 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6590 AflIII A'CryG_T Cuts at: 1203 5686 6663 1203 Size: 4483 977 1327 Fragments arranged by size: 4483 1327 977 AgeI A'CCGG_T Cuts at: 5618 5618 Size: 6787 AhdI GACnn_n'nnGTC Cuts at: 5516 5516 Size: 6787 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6787 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6787 AlwNI CAG_nnn'CTG Cuts at: 3436 4530 6102 3436 Size: 1094 1572 4121 Fragments arranged by size: 4121 1572 1094 ApaLI G'TGCA_C Cuts at: 2210 3190 3853 4844 6000 2210 Size: 980 663 991 1156 2997 Fragments arranged by size: 2997 1156 991 980 663 ApoI r'AATT_y Cuts at: 1493 4096 4322 4474 6583 1493 Size: 2603 226 152 2109 1697 Fragments arranged by size: 2603 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 3033 3033 Size: 6787 AseI AT'TA_AT Cuts at: 160 1032 5341 160 Size: 872 4309 1606 Fragments arranged by size: 4309 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6787 AvaI C'yCGr_G Cuts at: 1870 1943 2420 2693 3112 3802 4112 1870 Size: 73 477 273 419 690 310 4545 Fragments arranged by size: 4545 690 477 419 310 273 73 BamHI G'GATC_C Cuts at: 4117 4484 4117 Size: 367 6420 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2620 2697 3463 3491 3881 Size: 325 217 627 833 77 766 28 390 Cuts at: 3881 3905 4108 5563 618 Size: 24 203 1455 1842 Fragments arranged by size: 1842 1455 833 766 627 390 325 217 203 77 28 24 BanII G_rGCy'C Cuts at: 1391 2694 3995 4049 4106 1391 Size: 1303 1301 54 57 4072 Fragments arranged by size: 4072 1303 1301 57 54 BbeI G_GCGC'C Cuts at: 2624 3467 3885 3909 2624 Size: 843 418 24 5502 Fragments arranged by size: 5502 843 418 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2830 3193 3751 928 Size: 971 931 363 558 3964 Fragments arranged by size: 3964 971 931 558 363 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4978 5012 1903 Size: 34 3041 34 3678 Fragments arranged by size: 3678 3041 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4679 5889 4679 Size: 1210 5577 BclI T'GATC_A Cuts at: 1548 1548 Size: 6787 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2051 2090 2379 Size: 107 122 71 631 605 379 39 289 Cuts at: 2379 5398 136 Size: 3019 1525 Fragments arranged by size: 3019 1525 631 605 379 289 122 107 71 39 BglII A'GATC_T Cuts at: 6782 6782 Size: 6787 BlpI GC'TnA_GC Cuts at: 3380 4204 3380 Size: 824 5963 Bme1580I G_kGCm'C Cuts at: 1790 2214 3194 3351 3633 3857 4040 4848 6004 Size: 424 980 157 282 224 183 808 1156 Cuts at: 6004 1790 Size: 2573 Fragments arranged by size: 2573 1156 980 808 424 282 224 183 157 BmgBI CAC'GTC Cuts at: 1318 4026 1318 Size: 2708 4079 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4518 5476 442 Size: 416 53 83 399 24 3101 958 1753 Fragments arranged by size: 3101 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6787 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 3602 3854 5447 1239 Size: 986 1377 252 1593 2579 Fragments arranged by size: 2579 1593 1377 986 252 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2240 3752 4912 5777 6075 2240 Size: 1512 1160 865 298 2952 Fragments arranged by size: 2952 1512 1160 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3164 5450 882 Size: 617 1665 2286 2219 Fragments arranged by size: 2286 2219 1665 617 BsaAI yAC'GTr Cuts at: 493 1204 3160 3421 4578 493 Size: 711 1956 261 1157 2702 Fragments arranged by size: 2702 1956 1157 711 261 BsaBI GATnn'nnATC Cuts at: 6781 6781 Size: 6787 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2621 3464 3882 3906 Size: 53 83 186 936 1088 843 418 24 Cuts at: 3906 4008 4594 4976 5611 275 Size: 102 586 382 635 1451 Fragments arranged by size: 1451 1088 936 843 635 586 418 382 186 102 83 53 24 BsaWI w'CCGG_w Cuts at: 5220 5618 5892 6039 6450 5220 Size: 398 274 147 411 5557 Fragments arranged by size: 5557 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2225 2255 1178 Size: 30 165 30 822 30 5710 Fragments arranged by size: 5710 822 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3149 1186 Size: 1963 4824 BseYI C'CCAG_C Cuts at: 2486 2626 3053 3139 3350 3684 3867 5990 6486 Size: 140 427 86 211 334 183 2123 496 Cuts at: 6486 2486 Size: 2787 Fragments arranged by size: 2787 2123 496 427 334 211 183 140 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2290 3043 1163 Size: 573 554 753 4907 Fragments arranged by size: 4907 753 573 554 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2085 2398 2413 2554 3279 3915 Size: 275 186 84 313 15 141 725 636 Cuts at: 3915 4034 4237 4998 6026 1540 Size: 119 203 761 1028 2301 Fragments arranged by size: 2301 1028 761 725 636 313 275 203 186 141 119 84 15 BsiHKAI G_wGCw'C Cuts at: 2214 2246 2865 3194 3621 3857 4049 4848 4933 Size: 32 619 329 427 236 192 799 85 Cuts at: 4933 6004 2214 Size: 1071 2997 Fragments arranged by size: 2997 1071 799 619 427 329 236 192 85 32 BsmI GAATG_Cn' Cuts at: 4298 4391 4298 Size: 93 6694 BsmBI CGTCTCn'nnnn_ Cuts at: 2896 2896 Size: 6787 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2214 2246 2694 2865 3194 3351 3621 Size: 399 424 32 448 171 329 157 270 Cuts at: 3621 3633 3857 3995 4040 4049 4106 4848 4933 Size: 12 224 138 45 9 57 742 85 Cuts at: 4933 6004 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 448 424 399 329 270 224 171 157 138 85 57 45 32 12 9 BspEI T'CCGG_A Cuts at: 6450 6450 Size: 6787 BspHI T'CATG_A Cuts at: 4676 6406 4676 Size: 1730 5057 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3453 3729 4142 844 Size: 2609 276 413 3489 Fragments arranged by size: 3489 2609 413 276 BsrBI CCG'CTC Cuts at: 1394 1823 2328 2365 2746 2935 3537 4674 1394 Size: 429 505 37 381 189 602 1137 3507 Fragments arranged by size: 3507 1137 602 505 429 381 189 37 BsrDI GCAATG_nn' Cuts at: 65 1248 5282 5456 65 Size: 1183 4034 174 1396 Fragments arranged by size: 4034 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2351 2412 3176 3278 3308 3509 5431 Size: 110 541 61 764 102 30 201 1922 Cuts at: 5431 5618 1700 Size: 187 2869 Fragments arranged by size: 2869 1922 764 541 201 187 110 102 61 30 BsrGI T'GTAC_A Cuts at: 96 2277 96 Size: 2181 4606 BssHII G'CGCG_C Cuts at: 1925 2341 2358 2709 2784 2824 3033 1925 Size: 416 17 351 75 40 209 5679 Fragments arranged by size: 5679 416 351 209 75 40 17 BssSI C'ACGA_G Cuts at: 3008 4847 5859 3008 Size: 1839 1012 3936 Fragments arranged by size: 3936 1839 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2406 3160 3861 794 Size: 1612 754 701 3720 Fragments arranged by size: 3720 1612 754 701 BstBI TT'CG_AA Cuts at: 5591 5591 Size: 6787 BstEII G'GTnAC_C Cuts at: 1184 2015 1184 Size: 831 5956 BstXI CCAn_nnnn'nTGG Cuts at: 2101 2101 Size: 6787 BstYI r'GATC_y Cuts at: 1972 2235 4117 4484 4869 4886 6338 6424 6436 Size: 263 1882 367 385 17 1452 86 12 Cuts at: 6436 6782 1972 Size: 346 1977 Fragments arranged by size: 1977 1882 1452 385 367 346 263 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2548 2581 3195 3636 3885 3909 Size: 552 939 544 33 614 441 249 24 Cuts at: 3909 3915 513 Size: 6 3385 Fragments arranged by size: 3385 939 614 552 544 441 249 33 24 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3289 3349 487 Size: 683 56 2063 60 3925 Fragments arranged by size: 3925 2063 683 60 56 BtsI GCAGTG_nn' Cuts at: 4303 5097 5117 4303 Size: 794 20 5973 Fragments arranged by size: 5973 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6787 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6752 DraI TTT'AAA Cuts at: 4091 4091 Size: 6787 DraIII CAC_nnn'GTG Cuts at: 2445 2445 Size: 6787 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3755 3835 4030 5794 809 Size: 507 2439 80 195 1764 1802 Fragments arranged by size: 2439 1802 1764 507 195 80 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2082 2091 Size: 54 1425 50 275 18 213 39 9 Cuts at: 2091 2222 2545 2551 3228 3276 3333 3507 3912 Size: 131 323 6 677 48 57 174 405 Cuts at: 3912 4234 5123 6752 8 Size: 322 889 1629 43 Fragments arranged by size: 1629 1425 889 677 405 323 322 275 213 174 131 57 54 50 48 43 39 18 9 6 EagI C'GGCC_G Cuts at: 1537 1812 2082 2551 3276 3912 4234 1537 Size: 275 270 469 725 636 322 4090 Fragments arranged by size: 4090 725 636 469 322 275 270 EarI CTCTTCn'nnn_ Cuts at: 1353 2972 3488 3530 3662 4717 1353 Size: 1619 516 42 132 1055 3423 Fragments arranged by size: 3423 1619 1055 516 132 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3587 5374 5748 5894 387 Size: 1064 138 1998 1787 374 146 1280 Fragments arranged by size: 1998 1787 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4047 4047 Size: 6787 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 3602 3854 4850 5447 6234 6502 Size: 393 593 1377 252 996 597 787 268 Cuts at: 6502 1239 Size: 1524 Fragments arranged by size: 1524 1377 996 787 597 593 393 268 252 EcoO109I rG'GnC_Cy Cuts at: 2571 3718 3956 2571 Size: 1147 238 5402 Fragments arranged by size: 5402 1147 238 EcoRI G'AATT_C Cuts at: 4096 4474 4096 Size: 378 6409 FspI TGC'GCA Cuts at: 5293 5293 Size: 6787 HaeII r_GCGC'y Cuts at: 1678 2057 2068 2079 2624 2740 2920 3054 3380 Size: 379 11 11 545 116 180 134 326 Cuts at: 3380 3467 3847 3885 3909 5934 1678 Size: 87 380 38 24 2025 2531 Fragments arranged by size: 2531 2025 545 380 379 326 180 134 116 87 38 24 11 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5428 5460 5502 5534 1373 Size: 32 135 32 3856 32 42 32 2626 Fragments arranged by size: 3856 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4131 4377 669 Size: 988 281 2193 246 3079 Fragments arranged by size: 3079 2193 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6787 HpaI GTT'AAC Cuts at: 4377 4377 Size: 6787 KasI G'GCGC_C Cuts at: 2620 3463 3881 3905 2620 Size: 843 418 24 5502 Fragments arranged by size: 5502 843 418 24 KpnI G_GTAC'C Cuts at: 4112 4112 Size: 6787 MfeI C'AATT_G Cuts at: 4386 4386 Size: 6787 MluI A'CGCG_T Cuts at: 6663 6663 Size: 6787 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2004 5901 6085 1305 Size: 699 3897 184 2007 Fragments arranged by size: 3897 2007 699 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3230 10 Size: 54 1425 343 1398 3567 Fragments arranged by size: 3567 1425 1398 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2159 3368 4745 5104 5263 518 Size: 648 993 1209 1377 359 159 2042 Fragments arranged by size: 2042 1377 1209 993 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2128 2227 2314 2544 2583 3027 3469 3638 Size: 522 99 87 230 39 444 442 169 Cuts at: 3638 3861 3887 3917 4203 4880 6028 6273 1606 Size: 223 26 30 286 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 522 444 442 286 245 230 223 169 99 87 39 30 26 NaeI GCC'GGC Cuts at: 2353 3310 3511 2353 Size: 957 201 5629 Fragments arranged by size: 5629 957 201 NarI GG'CG_CC Cuts at: 2621 3464 3882 3906 2621 Size: 843 418 24 5502 Fragments arranged by size: 5502 843 418 24 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5296 Fragments arranged by size: 5296 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6787 NgoMIV G'CCGG_C Cuts at: 2351 3308 3509 2351 Size: 957 201 5629 Fragments arranged by size: 5629 957 201 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6787 NotI GC'GGCC_GC Cuts at: 2082 4234 2082 Size: 2152 4635 NspI r_CATG'y Cuts at: 1132 1229 2109 2164 2278 2404 4145 5690 1132 Size: 97 880 55 114 126 1741 1545 2229 Fragments arranged by size: 2229 1741 1545 880 126 114 97 55 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6787 PciI A'CATG_T Cuts at: 5686 5686 Size: 6787 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 3243 3436 1108 Size: 902 1233 193 4459 Fragments arranged by size: 4459 1233 902 193 PmeI GTTT'AAAC Cuts at: 4091 4091 Size: 6787 PmlI CAC'GTG Cuts at: 3160 3160 Size: 6787 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4856 4888 6369 6401 4856 Size: 32 1481 32 5242 Fragments arranged by size: 5242 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3718 3956 3718 Size: 238 6549 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6787 PsiI TTA'TAA Cuts at: 4357 4357 Size: 6787 PstI C_TGCA'G Cuts at: 830 2064 3726 4139 830 Size: 1234 1662 413 3478 Fragments arranged by size: 3478 1662 1234 413 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6787 SacI G_AGCT'C Cuts at: 4049 4049 Size: 6787 SacII CC_GC'GG Cuts at: 2584 3639 3888 3918 2584 Size: 1055 249 30 5453 Fragments arranged by size: 5453 1055 249 30 SalI G'TCGA_C Cuts at: 1936 4129 1936 Size: 2193 4594 SanDI GG'GwC_CC Cuts at: 3956 3956 Size: 6787 SbfI CC_TGCA'GG Cuts at: 4139 4139 Size: 6787 ScaI AGT'ACT Cuts at: 3323 3773 5035 6747 3323 Size: 450 1262 1712 3363 Fragments arranged by size: 3363 1712 1262 450 SfcI C'TryA_G Cuts at: 826 1045 2060 3722 4000 4135 5270 5951 6142 Size: 219 1015 1662 278 135 1135 681 191 Cuts at: 6142 826 Size: 1471 Fragments arranged by size: 1662 1471 1135 1015 681 278 219 191 135 SfiI GGCCn_nnn'nGGCC Cuts at: 2090 2090 Size: 6787 SfoI GGC'GCC Cuts at: 2622 3465 3883 3907 2622 Size: 843 418 24 5502 Fragments arranged by size: 5502 843 418 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6787 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6787 SmaI CCC'GGG Cuts at: 1872 2695 3114 4114 1872 Size: 823 419 1000 4545 Fragments arranged by size: 4545 1000 823 419 SmlI C'TyrA_G Cuts at: 820 1017 1943 2255 3767 4891 5792 6054 820 Size: 197 926 312 1512 1124 901 262 1553 Fragments arranged by size: 1553 1512 1124 926 901 312 262 197 SnaBI TAC'GTA Cuts at: 493 4578 493 Size: 4085 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6787 SphI G_CATG'C Cuts at: 1132 2109 2404 4145 1132 Size: 977 295 1741 3774 Fragments arranged by size: 3774 1741 977 295 SrfI GCCC'GGGC Cuts at: 3114 3114 Size: 6787 SspI AAT'ATT Cuts at: 5 52 4711 5 Size: 47 4659 2081 Fragments arranged by size: 4659 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6787 StyI C'CwwG_G Cuts at: 513 1065 2004 2049 2094 2346 4226 513 Size: 552 939 45 45 252 1880 3074 Fragments arranged by size: 3074 1880 939 552 252 45 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3235 3529 4814 4831 4984 5169 5614 Size: 1255 1655 294 1285 17 153 185 445 Cuts at: 5614 325 Size: 1498 Fragments arranged by size: 1655 1498 1285 1255 445 294 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2277 3321 3771 Size: 274 80 33 51 1443 300 1044 450 Cuts at: 3771 5033 6745 96 Size: 1262 1712 138 Fragments arranged by size: 1712 1443 1262 1044 450 300 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2225 2257 6747 6779 2225 Size: 32 4490 32 2233 Fragments arranged by size: 4490 2233 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6787 XbaI T'CTAG_A Cuts at: 4123 4123 Size: 6787 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3370 3502 3370 Size: 132 6655 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6787 XmaI C'CCGG_G Cuts at: 1870 2693 3112 4112 1870 Size: 823 419 1000 4545 Fragments arranged by size: 4545 1000 823 419 XmnI GAAnn'nnTTC Cuts at: 4916 4916 Size: 6787 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4009 4595 276 Size: 53 83 186 936 2475 586 2468 Fragments arranged by size: 2475 2468 936 586 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SapI SexAI SwaI