Restriction map for
pFN21AB7201
(Linear) (Six-base) MAPSORT of: KIBB7201.seq Check: 3631 from: 1 to: 2082
KIBB7201.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AatII G_ACGT'C
Cuts at: 0 2006 2082
Size: 2006 76
AccI GT'mk_AC
Cuts at: 0 1208 2082
Size: 1208 874
AfeI AGC'GCT
Cuts at: 0 61 913 2082
Size: 61 852 1169
Fragments arranged by size:
1169 852 61
AlwNI CAG_nnn'CTG
Cuts at: 0 1431 2082
Size: 1431 651
ApaLI G'TGCA_C
Cuts at: 0 205 1185 1848 2082
Size: 205 980 663 234
Fragments arranged by size:
980 663 234 205
AscI GG'CGCG_CC
Cuts at: 0 1028 2082
Size: 1028 1054
AvaI C'yCGr_G
Cuts at: 0 415 688 1107 1797 2082
Size: 415 273 419 690 285
Fragments arranged by size:
690 419 415 285 273
BanI G'GyrC_C
Cuts at: 0 615 692 1458 1486 1876 1900 2082
Size: 615 77 766 28 390 24 182
Fragments arranged by size:
766 615 390 182 77 28 24
BanII G_rGCy'C
Cuts at: 0 689 1990 2044 2082
Size: 689 1301 54 38
Fragments arranged by size:
1301 689 54 38
BbeI G_GCGC'C
Cuts at: 0 619 1462 1880 1904 2082
Size: 619 843 418 24 178
Fragments arranged by size:
843 619 418 178 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 825 1188 1746 2082
Size: 825 363 558 336
Fragments arranged by size:
825 558 363 336
BglI GCCn_nnn'nGGC
Cuts at: 0 46 85 374 2082
Size: 46 39 289 1708
Fragments arranged by size:
1708 289 46 39
BlpI GC'TnA_GC
Cuts at: 0 1375 2082
Size: 1375 707
Bme1580I G_kGCm'C
Cuts at: 0 209 1189 1346 1628 1852 2035 2082
Size: 209 980 157 282 224 183 47
Fragments arranged by size:
980 282 224 209 183 157 47
BmgBI CAC'GTC
Cuts at: 0 2021 2082
Size: 2021 61
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 1597 1849 2082
Size: 220 1377 252 233
Fragments arranged by size:
1377 252 233 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1747 2082
Size: 235 1512 335
Fragments arranged by size:
1512 335 235
BsaI GGTCTCn'nnnn_
Cuts at: 0 1159 2082
Size: 1159 923
BsaAI yAC'GTr
Cuts at: 0 1155 1416 2082
Size: 1155 261 666
Fragments arranged by size:
1155 666 261
BsaHI Gr'CG_yC
Cuts at: 0 616 1459 1877 1901 2003 2082
Size: 616 843 418 24 102 79
Fragments arranged by size:
843 616 418 102 79 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 220 250 2082
Size: 220 30 1832
Fragments arranged by size:
1832 220 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1144 2082
Size: 1144 938
BseYI C'CCAG_C
Cuts at: 0 481 621 1048 1134 1345 1679 1862 2082
Size: 481 140 427 86 211 334 183 220
Fragments arranged by size:
481 427 334 220 211 183 140 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 1038 2082
Size: 285 753 1044
Fragments arranged by size:
1044 753 285
BsiEI CG_ry'CG
Cuts at: 0 80 393 408 549 1274 1910 2029 2082
Size: 80 313 15 141 725 636 119 53
Fragments arranged by size:
725 636 313 141 119 80 53 15
BsiHKAI G_wGCw'C
Cuts at: 0 209 241 860 1189 1616 1852 2044 2082
Size: 209 32 619 329 427 236 192 38
Fragments arranged by size:
619 427 329 236 209 192 38 32
BsmBI CGTCTCn'nnnn_
Cuts at: 0 891 2082
Size: 891 1191
Bsp1286I G_dGCh'C
Cuts at: 0 209 241 689 860 1189 1346 1616 1628
Size: 209 32 448 171 329 157 270 12
Cuts at: 1628 1852 1990 2035 2044 2082
Size: 224 138 45 9 38
Fragments arranged by size:
448 329 270 224 209 171 157 138
45 38 32 12 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1448 1724 2082
Size: 1448 276 358
Fragments arranged by size:
1448 358 276
BsrBI CCG'CTC
Cuts at: 0 323 360 741 930 1532 2082
Size: 323 37 381 189 602 550
Fragments arranged by size:
602 550 381 323 189 37
BsrFI r'CCGG_y
Cuts at: 0 346 407 1171 1273 1303 1504 2082
Size: 346 61 764 102 30 201 578
Fragments arranged by size:
764 578 346 201 102 61 30
BsrGI T'GTAC_A
Cuts at: 0 272 2082
Size: 272 1810
BssHII G'CGCG_C
Cuts at: 0 336 353 704 779 819 1028 2082
Size: 336 17 351 75 40 209 1054
Fragments arranged by size:
1054 351 336 209 75 40 17
BssSI C'ACGA_G
Cuts at: 0 1003 2082
Size: 1003 1079
BstAPI GCAn_nnn'nTGC
Cuts at: 0 401 1155 1856 2082
Size: 401 754 701 226
Fragments arranged by size:
754 701 401 226
BstEII G'GTnAC_C
Cuts at: 0 10 2082
Size: 10 2072
BstXI CCAn_nnnn'nTGG
Cuts at: 0 96 2082
Size: 96 1986
BstYI r'GATC_y
Cuts at: 0 230 2082
Size: 230 1852
BtgI C'CryG_G
Cuts at: 0 543 576 1190 1631 1880 1904 1910 2082
Size: 543 33 614 441 249 24 6 172
Fragments arranged by size:
614 543 441 249 172 33 24 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1284 1344 2082
Size: 1284 60 738
Fragments arranged by size:
1284 738 60
DraIII CAC_nnn'GTG
Cuts at: 0 440 2082
Size: 440 1642
DrdI GACnn_nn'nnGTC
Cuts at: 0 1750 1830 2025 2082
Size: 1750 80 195 57
Fragments arranged by size:
1750 195 80 57
EaeI y'GGCC_r
Cuts at: 0 38 77 86 217 540 546 1223 1271
Size: 38 39 9 131 323 6 677 48
Cuts at: 1271 1328 1502 1907 2082
Size: 57 174 405 175
Fragments arranged by size:
677 405 323 175 174 131 57 48
39 38 9 6
EagI C'GGCC_G
Cuts at: 0 77 546 1271 1907 2082
Size: 77 469 725 636 175
Fragments arranged by size:
725 636 469 175 77
EarI CTCTTCn'nnn_
Cuts at: 0 967 1483 1525 1657 2082
Size: 967 516 42 132 425
Fragments arranged by size:
967 516 425 132 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1582 2082
Size: 1582 500
EcoICRI GAG'CTC
Cuts at: 0 2042 2082
Size: 2042 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 1597 1849 2082
Size: 220 1377 252 233
Fragments arranged by size:
1377 252 233 220
EcoO109I rG'GnC_Cy
Cuts at: 0 566 1713 1951 2082
Size: 566 1147 238 131
Fragments arranged by size:
1147 566 238 131
HaeII r_GCGC'y
Cuts at: 0 52 63 74 619 735 915 1049 1375
Size: 52 11 11 545 116 180 134 326
Cuts at: 1375 1462 1842 1880 1904 2082
Size: 87 380 38 24 178
Fragments arranged by size:
545 380 326 180 178 134 116 87
52 38 24 11 11
KasI G'GCGC_C
Cuts at: 0 615 1458 1876 1900 2082
Size: 615 843 418 24 182
Fragments arranged by size:
843 615 418 182 24
MscI TGG'CCA
Cuts at: 0 1225 2082
Size: 1225 857
MslI CAynn'nnrTG
Cuts at: 0 154 1363 2082
Size: 154 1209 719
Fragments arranged by size:
1209 719 154
MspA1I CmG'CkG
Cuts at: 0 123 222 309 539 578 1022 1464 1633
Size: 123 99 87 230 39 444 442 169
Cuts at: 1633 1856 1882 1912 2082
Size: 223 26 30 170
Fragments arranged by size:
444 442 230 223 170 169 123 99
87 39 30 26
NaeI GCC'GGC
Cuts at: 0 348 1305 1506 2082
Size: 348 957 201 576
Fragments arranged by size:
957 576 348 201
NarI GG'CG_CC
Cuts at: 0 616 1459 1877 1901 2082
Size: 616 843 418 24 181
Fragments arranged by size:
843 616 418 181 24
NgoMIV G'CCGG_C
Cuts at: 0 346 1303 1504 2082
Size: 346 957 201 578
Fragments arranged by size:
957 578 346 201
NotI GC'GGCC_GC
Cuts at: 0 77 2082
Size: 77 2005
NspI r_CATG'y
Cuts at: 0 104 159 273 399 2082
Size: 104 55 114 126 1683
Fragments arranged by size:
1683 126 114 104 55
PflMI CCAn_nnn'nTGG
Cuts at: 0 1238 1431 2082
Size: 1238 193 651
Fragments arranged by size:
1238 651 193
PmlI CAC'GTG
Cuts at: 0 1155 2082
Size: 1155 927
PpuMI rG'GwC_Cy
Cuts at: 0 1713 1951 2082
Size: 1713 238 131
Fragments arranged by size:
1713 238 131
PstI C_TGCA'G
Cuts at: 0 59 1721 2082
Size: 59 1662 361
Fragments arranged by size:
1662 361 59
SacI G_AGCT'C
Cuts at: 0 2044 2082
Size: 2044 38
SacII CC_GC'GG
Cuts at: 0 579 1634 1883 1913 2082
Size: 579 1055 249 30 169
Fragments arranged by size:
1055 579 249 169 30
SanDI GG'GwC_CC
Cuts at: 0 1951 2082
Size: 1951 131
ScaI AGT'ACT
Cuts at: 0 1318 1768 2082
Size: 1318 450 314
Fragments arranged by size:
1318 450 314
SfcI C'TryA_G
Cuts at: 0 55 1717 1995 2082
Size: 55 1662 278 87
Fragments arranged by size:
1662 278 87 55
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 85 2082
Size: 85 1997
SfoI GGC'GCC
Cuts at: 0 617 1460 1878 1902 2082
Size: 617 843 418 24 180
Fragments arranged by size:
843 617 418 180 24
SmaI CCC'GGG
Cuts at: 0 690 1109 2082
Size: 690 419 973
Fragments arranged by size:
973 690 419
SmlI C'TyrA_G
Cuts at: 0 250 1762 2082
Size: 250 1512 320
Fragments arranged by size:
1512 320 250
SphI G_CATG'C
Cuts at: 0 104 399 2082
Size: 104 295 1683
Fragments arranged by size:
1683 295 104
SrfI GCCC'GGGC
Cuts at: 0 1109 2082
Size: 1109 973
StyI C'CwwG_G
Cuts at: 0 44 89 341 2082
Size: 44 45 252 1741
Fragments arranged by size:
1741 252 45 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1230 1524 2082
Size: 1230 294 558
Fragments arranged by size:
1230 558 294
TatI w'GTAC_w
Cuts at: 0 272 1316 1766 2082
Size: 272 1044 450 316
Fragments arranged by size:
1044 450 316 272
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 220 252 2082
Size: 220 32 1830
Fragments arranged by size:
1830 220 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1365 1497 2082
Size: 1365 132 585
Fragments arranged by size:
1365 585 132
XmaI C'CCGG_G
Cuts at: 0 688 1107 2082
Size: 688 419 975
Fragments arranged by size:
975 688 419
ZraI GAC'GTC
Cuts at: 0 2004 2082
Size: 2004 78
Enzymes that do cut:
AatII AccI AfeI AlwNI ApaLI AscI AvaI BanI
BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BpmI
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I HaeII KasI MscI MslI MspA1I NaeI NarI
NgoMIV NotI NspI PflMI PmlI PpuMI PstI SacI
SacII SanDI ScaI SfcI SfiI SfoI SmaI SmlI
SphI SrfI StyI TaqII TatI TstI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApoI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BmrI BmtI BplI Bpu10I BsaBI BsaWI BsiWI BsmI
BspEI BspHI BsrDI BstBI BstZ17I Bsu36I BtsI ClaI
CspCI DraI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI NcoI NdeI NheI NruI NsiI PacI PasI
PciI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SalI SapI SbfI SexAI
SgfI SgrAI SnaBI SpeI SspI StuI SwaI Tth111I
XbaI XhoI XmnI