################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## Acc65I AccIII AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstBI BstEII BstPI BstYI Bsu36I CelII Cfr10I ClaI CpoI DraIII EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO65I EcoRI EcoT22I FauI FseI FspI HpaI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MwoI NaeI NarI NciI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 1285 GACGTC GACGT^C AccI 1428 GTATAC GT^[AC][GT]AC AccII 2232 CGCG CG^CG AciI 361 GCGG CCGC(-3/-1) AclI 1322 AACGTT AA^CGTT AlwNI 937 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1447 AGCGCT AGC^GCT BsaWI 2024 ACCGGA [AT]^CCGG[AT] BsmAI 624 GTCTC GTCTC(1/5) BssSI 1245 CACGAG CACGAG(-5/-1) Bst1107I 1428 GTATAC GTA^TAC BstUI 2232 CGCG CG^CG BstXI 1343 CCATGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1428 GTATAC GTA^TAC CfoI 1448 GCGC GCG^C DraI 1951 TTTAAA TTT^AAA DrdI 1279 GACAAGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1447 AGCGCT AGC^GCT EcoNI 987 CCTTACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 121 GATATC GAT^ATC HaeII 1447 AGCGCT [GA]GCGC^[CT] HapII 2025 CCGG C^CGG HgaI 2206 GCGTC GACGC(5/10) HhaI 1448 GCGC GCG^C HinP1I 1448 GCGC G^CGC HincII 797 GTCAAC GT[CT]^[GA]AC HindII 797 GTCAAC GT[CT]^[GA]AC HindIII 665 AAGCTT A^AGCTT HpaII 2025 CCGG C^CGG MspI 2025 CCGG C^CGG MvnI 2232 CGCG CG^CG NheI 423 GCTAGC G^CTAGC NlaIV 1563 GGCTCC GG[ACGT]^[ACGT]CC Psp1406I 1322 AACGTT AA^CGTT SfaNI 1897 GATGC GCATC(5/9) Tsp45I 1184 GTGAC ^GT[GC]AC XcmI 1635 CCAACATTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 1285 GACGTC G[GA]^CG[CT]C 2205 GGCGTC AvaII 363 GGACC G^G[AT]CC 2022 GGACC BbsI 520 GAAGAC GAAGAC(2/6) 1234 GAAGAC BclI 1080 TGATCA T^GATCA 1370 TGATCA BpuAI 520 GAAGAC GAAGAC(2/6) 1234 GAAGAC BsaHI 1285 GACGTC G[GA]^CG[CT]C 2205 GGCGTC BsgI 290 GTGCAG GTGCAG(16/14) 2009 GTGCAG BsmI 482 GAATGC GAATGC(1/-1) 1310 GAATGC Bsp1407I 1608 TGTACA T^GTACA 1678 TGTACA BspHI 789 TCATGA T^CATGA 2060 TCATGA BsrGI 1608 TGTACA T^GTACA 1678 TGTACA BssKI 1590 CCAGG ^CC[ACGT]GG 2202 CCAGG BstNI 1590 CCAGG CC^[AT]GG 2202 CCAGG DraII 965 AGGGCCT [GA]G^G[ACGT]CC[CT] 1109 AGGCCCT EcoO109I 965 AGGGCCT [GA]G^G[ACGT]CC[CT] 1109 AGGCCCT EcoRII 1590 CCAGG ^CC[AT]GG 2202 CCAGG FbaI 1080 TGATCA T^GATCA 1370 TGATCA Hin1I 1285 GACGTC G[GA]^CG[CT]C 2205 GGCGTC MvaI 1590 CCAGG CC^[AT]GG 2202 CCAGG NcoI 543 CCATGG C^CATGG 1724 CCATGG NdeI 928 CATATG CA^TATG 1715 CATATG NspI 834 ACATGC [GA]CATG^[CT] 1001 ACATGC RcaI 789 TCATGA T^CATGA 2060 TCATGA ScaI 246 AGTACT AGT^ACT 1178 AGTACT ScrFI 1590 CCAGG CC^[ACGT]GG 2202 CCAGG SfcI 1534 CTACAG C^T[GA][CT]AG 2048 CTATAG SspBI 1608 TGTACA T^GTACA 1678 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AcsI AfaI AluI ApoI Asp700I BbvI BfaI BsaJI BsiYI BslI BsrI BstF5I Cac8I Cfr13I DdeI DpnII DsaI EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI NdeII NlaIII PleI RsaI Sau3AI Sau96I StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XmnI