(Circular) (Six-base) MAPSORT of: pFN21AE2077.seq Check: 7510 from: 1 to: 6940 pFN21AE2077 6940 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3294 4750 278 Size: 53 83 186 936 1758 1456 2468 Fragments arranged by size: 2468 1758 1456 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3434 4283 1937 Size: 1497 849 4594 Fragments arranged by size: 4594 1497 849 Acc65I G'GTAC_C Cuts at: 4261 4261 Size: 6940 AclI AA'CG_TT Cuts at: 1563 3328 5067 5440 1563 Size: 1765 1739 373 3063 Fragments arranged by size: 3063 1765 1739 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2357 2453 3221 3384 3785 5003 6387 6655 Size: 725 96 768 163 401 1218 1384 268 Cuts at: 6655 1632 Size: 1917 Fragments arranged by size: 1917 1384 1218 768 725 401 268 163 96 AfeI AGC'GCT Cuts at: 3454 3454 Size: 6940 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6743 AflIII A'CryG_T Cuts at: 1203 5839 6816 1203 Size: 4636 977 1327 Fragments arranged by size: 4636 1327 977 AgeI A'CCGG_T Cuts at: 5771 5771 Size: 6940 AhdI GACnn_n'nnGTC Cuts at: 5669 5669 Size: 6940 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6940 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6940 AlwNI CAG_nnn'CTG Cuts at: 2947 4683 6255 2947 Size: 1736 1572 3632 Fragments arranged by size: 3632 1736 1572 ApaLI G'TGCA_C Cuts at: 4997 6153 4997 Size: 1156 5784 ApoI r'AATT_y Cuts at: 1493 2594 2619 2677 2745 3050 3953 4249 4475 Size: 1101 25 58 68 305 903 296 226 Cuts at: 4475 4627 6736 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1101 903 305 296 226 152 68 58 25 AseI AT'TA_AT Cuts at: 160 1032 5494 160 Size: 872 4462 1606 Fragments arranged by size: 4462 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6940 AvaI C'yCGr_G Cuts at: 1870 1943 4265 1870 Size: 73 2322 4545 Fragments arranged by size: 4545 2322 73 BamHI G'GATC_C Cuts at: 4270 4637 4270 Size: 367 6573 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4261 5716 618 Size: 325 217 627 2474 1455 1842 Fragments arranged by size: 2474 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4259 1391 Size: 2868 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2532 3246 928 Size: 971 633 714 4622 Fragments arranged by size: 4622 971 714 633 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5131 5165 1903 Size: 34 3194 34 3678 Fragments arranged by size: 3678 3194 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4832 6042 4832 Size: 1210 5730 BclI T'GATC_A Cuts at: 1548 3085 3375 1548 Size: 1537 290 5113 Fragments arranged by size: 5113 1537 290 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5551 136 Size: 107 122 71 631 605 3879 1525 Fragments arranged by size: 3879 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6935 6935 Size: 6940 BlpI GC'TnA_GC Cuts at: 4357 4357 Size: 6940 Bme1580I G_kGCm'C Cuts at: 1790 5001 6157 1790 Size: 3211 1156 2573 Fragments arranged by size: 3211 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6940 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2284 2329 4137 Size: 416 53 83 399 24 867 45 1808 Cuts at: 4137 4671 5629 442 Size: 534 958 1753 Fragments arranged by size: 1808 1753 958 867 534 416 399 83 53 45 24 BmtI G_CTAG'C Cuts at: 1056 2432 1056 Size: 1376 5564 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5600 1239 Size: 4361 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5065 5930 6228 5065 Size: 865 298 5777 Fragments arranged by size: 5777 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5603 882 Size: 617 4104 2219 Fragments arranged by size: 4104 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4731 493 Size: 711 3527 2702 Fragments arranged by size: 3527 2702 711 BsaBI GATnn'nnATC Cuts at: 6934 6934 Size: 6940 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3291 4211 4747 5129 Size: 53 83 186 936 1758 920 536 382 Cuts at: 5129 5764 275 Size: 635 1451 Fragments arranged by size: 1758 1451 936 920 635 536 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4029 5373 5771 6045 6192 6603 4029 Size: 1344 398 274 147 411 4366 Fragments arranged by size: 4366 1344 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3553 3583 1178 Size: 30 165 30 2150 30 4535 Fragments arranged by size: 4535 2150 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6940 BseYI C'CCAG_C Cuts at: 6143 6639 6143 Size: 496 6444 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2316 4035 1163 Size: 573 580 1719 4068 Fragments arranged by size: 4068 1719 580 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4390 5151 6179 1540 Size: 275 186 2389 761 1028 2301 Fragments arranged by size: 2389 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5001 5086 6157 5001 Size: 85 1071 5784 Fragments arranged by size: 5784 1071 85 BsmI GAATG_Cn' Cuts at: 2493 3321 4451 4544 2493 Size: 828 1130 93 4889 Fragments arranged by size: 4889 1130 828 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4259 5001 5086 6157 1391 Size: 399 2469 742 85 1071 2174 Fragments arranged by size: 2469 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6603 6603 Size: 6940 BspHI T'CATG_A Cuts at: 2794 4065 4829 6559 2794 Size: 1271 764 1730 3175 Fragments arranged by size: 3175 1730 1271 764 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4295 844 Size: 3451 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4827 1394 Size: 429 3004 3507 Fragments arranged by size: 3507 3004 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5435 5609 65 Size: 1183 4187 174 1396 Fragments arranged by size: 4187 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5584 5771 1700 Size: 110 3774 187 2869 Fragments arranged by size: 3774 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3613 3683 96 Size: 3517 70 3353 Fragments arranged by size: 3517 3353 70 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6940 BssSI C'ACGA_G Cuts at: 3250 5000 6012 3250 Size: 1750 1012 4178 Fragments arranged by size: 4178 1750 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6940 BstBI TT'CG_AA Cuts at: 5744 5744 Size: 6940 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6940 BstXI CCAn_nnnn'nTGG Cuts at: 3355 3355 Size: 6940 BstYI r'GATC_y Cuts at: 1972 4270 4637 5022 5039 6491 6577 6589 6935 Size: 2298 367 385 17 1452 86 12 346 Cuts at: 6935 1972 Size: 1977 Fragments arranged by size: 2298 1977 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3435 3435 Size: 6940 BtgI C'CryG_G Cuts at: 513 1065 2073 2548 3729 513 Size: 552 1008 475 1181 3724 Fragments arranged by size: 3724 1181 1008 552 475 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6201 Fragments arranged by size: 6201 683 56 BtsI GCAGTG_nn' Cuts at: 4456 5250 5270 4456 Size: 794 20 6126 Fragments arranged by size: 6126 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6940 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6905 DraI TTT'AAA Cuts at: 3958 4244 3958 Size: 286 6654 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3290 5947 809 Size: 507 1974 2657 1802 Fragments arranged by size: 2657 1974 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4387 5276 6905 Size: 54 1425 50 275 18 2557 889 1629 Cuts at: 6905 8 Size: 43 Fragments arranged by size: 2557 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4387 1537 Size: 275 2575 4090 Fragments arranged by size: 4090 2575 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4870 1353 Size: 3517 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5527 5901 6047 387 Size: 1064 138 3938 374 146 1280 Fragments arranged by size: 3938 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2357 2453 3221 3384 3785 5003 5600 Size: 393 725 96 768 163 401 1218 597 Cuts at: 5600 6387 6655 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1218 787 768 725 597 401 393 268 163 96 EcoNI CCTnn'n_nnAGG Cuts at: 2996 2996 Size: 6940 EcoO109I rG'GnC_Cy Cuts at: 2971 3115 2971 Size: 144 6796 EcoRI G'AATT_C Cuts at: 4249 4627 4249 Size: 378 6562 EcoRV GAT'ATC Cuts at: 2128 2128 Size: 6940 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2387 3310 2387 Size: 923 6017 FspI TGC'GCA Cuts at: 5446 5446 Size: 6940 HaeII r_GCGC'y Cuts at: 1678 3456 6087 1678 Size: 1778 2631 2531 Fragments arranged by size: 2631 2531 1778 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2105 2137 3893 3925 5581 Size: 32 135 32 533 32 1756 32 1656 Cuts at: 5581 5613 5655 5687 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1756 1656 533 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2804 4284 4530 669 Size: 988 281 866 1480 246 3079 Fragments arranged by size: 3079 1480 988 866 281 246 HindIII A'AGCT_T Cuts at: 748 2670 748 Size: 1922 5018 HpaI GTT'AAC Cuts at: 4530 4530 Size: 6940 KpnI G_GTAC'C Cuts at: 4265 4265 Size: 6940 MfeI C'AATT_G Cuts at: 4539 4539 Size: 6940 MluI A'CGCG_T Cuts at: 6816 6816 Size: 6940 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2064 3087 6054 6238 1305 Size: 759 1023 2967 184 2007 Fragments arranged by size: 2967 2007 1023 759 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5118 Fragments arranged by size: 5118 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2961 3263 3353 3648 3815 4898 5257 Size: 648 1795 302 90 295 167 1083 359 Cuts at: 5257 5416 518 Size: 159 2042 Fragments arranged by size: 2042 1795 1083 648 359 302 295 167 159 90 MspA1I CmG'CkG Cuts at: 1606 4356 5033 6181 6426 1606 Size: 2750 677 1148 245 2120 Fragments arranged by size: 2750 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2548 3729 513 Size: 552 1483 1181 3724 Fragments arranged by size: 3724 1483 1181 552 NdeI CA'TA_TG Cuts at: 387 2934 3721 387 Size: 2547 787 3606 Fragments arranged by size: 3606 2547 787 NheI G'CTAG_C Cuts at: 1052 2428 1052 Size: 1376 5564 NotI GC'GGCC_GC Cuts at: 4387 4387 Size: 6940 NspI r_CATG'y Cuts at: 1132 1229 2843 3010 4298 5843 1132 Size: 97 1614 167 1288 1545 2229 Fragments arranged by size: 2229 1614 1545 1288 167 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6940 PciI A'CATG_T Cuts at: 5839 5839 Size: 6940 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6940 PmeI GTTT'AAAC Cuts at: 4244 4244 Size: 6940 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5009 5041 6522 6554 5009 Size: 32 1481 32 5395 Fragments arranged by size: 5395 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6940 PsiI TTA'TAA Cuts at: 3792 4510 3792 Size: 718 6222 PstI C_TGCA'G Cuts at: 830 4292 830 Size: 3462 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6940 SalI G'TCGA_C Cuts at: 1936 4282 1936 Size: 2346 4594 SbfI CC_TGCA'GG Cuts at: 4292 4292 Size: 6940 ScaI AGT'ACT Cuts at: 2253 3185 5188 6900 2253 Size: 932 2003 1712 2293 Fragments arranged by size: 2293 2003 1712 932 SfcI C'TryA_G Cuts at: 826 1045 3539 4053 4288 5423 6104 6295 826 Size: 219 2494 514 235 1135 681 191 1471 Fragments arranged by size: 2494 1471 1135 681 514 235 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6940 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6940 SmaI CCC'GGG Cuts at: 1872 4267 1872 Size: 2395 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5044 5945 6207 820 Size: 197 926 3101 901 262 1553 Fragments arranged by size: 3101 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4731 493 Size: 4238 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6940 SphI G_CATG'C Cuts at: 1132 4298 1132 Size: 3166 3774 SspI AAT'ATT Cuts at: 5 52 4864 5 Size: 47 4812 2081 Fragments arranged by size: 4812 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6940 StyI C'CwwG_G Cuts at: 513 1065 2161 2548 3729 4379 513 Size: 552 1096 387 1181 650 3074 Fragments arranged by size: 3074 1181 1096 650 552 387 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2348 4967 4984 5137 5322 5767 325 Size: 1255 768 2619 17 153 185 445 1498 Fragments arranged by size: 2619 1498 1255 768 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2251 3183 3613 Size: 274 80 33 51 1443 274 932 430 Cuts at: 3613 3683 5186 6898 96 Size: 70 1503 1712 138 Fragments arranged by size: 1712 1503 1443 932 430 274 274 138 80 70 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6900 6932 6900 Size: 32 6908 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6940 XbaI T'CTAG_A Cuts at: 4276 4276 Size: 6940 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3647 3647 Size: 6940 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6940 XmaI C'CCGG_G Cuts at: 1870 4265 1870 Size: 2395 4545 XmnI GAAnn'nnTTC Cuts at: 2466 2594 2718 2745 5069 2466 Size: 128 124 27 2324 4337 Fragments arranged by size: 4337 2324 128 124 27 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3292 4748 276 Size: 53 83 186 936 1758 1456 2468 Fragments arranged by size: 2468 1758 1456 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI