(Circular) (Six-base) MAPSORT of: pFN21AE5781.seq Check: 4023 from: 1 to: 6637 pFN21AE5781 6637 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4447 278 Size: 53 83 186 936 2911 2468 Fragments arranged by size: 2911 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2988 3267 3980 1937 Size: 1051 279 713 4594 Fragments arranged by size: 4594 1051 713 279 Acc65I G'GTAC_C Cuts at: 2115 3958 2115 Size: 1843 4794 AclI AA'CG_TT Cuts at: 1563 4764 5137 1563 Size: 3201 373 3063 Fragments arranged by size: 3201 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2331 4700 6084 6352 1632 Size: 699 2369 1384 268 1917 Fragments arranged by size: 2369 1917 1384 699 268 AfeI AGC'GCT Cuts at: 2918 2918 Size: 6637 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6440 AflIII A'CryG_T Cuts at: 1203 5536 6513 1203 Size: 4333 977 1327 Fragments arranged by size: 4333 1327 977 AgeI A'CCGG_T Cuts at: 5468 5468 Size: 6637 AhdI GACnn_n'nnGTC Cuts at: 5366 5366 Size: 6637 AleI CACnn'nnGTG Cuts at: 1166 2154 3878 1166 Size: 988 1724 3925 Fragments arranged by size: 3925 1724 988 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3896 1184 Size: 2712 3925 AlwNI CAG_nnn'CTG Cuts at: 2247 3372 4380 5952 2247 Size: 1125 1008 1572 2932 Fragments arranged by size: 2932 1572 1125 1008 ApaLI G'TGCA_C Cuts at: 4694 5850 4694 Size: 1156 5481 ApoI r'AATT_y Cuts at: 1493 2369 2421 2567 2859 3946 4172 4324 6433 Size: 876 52 146 292 1087 226 152 2109 Cuts at: 6433 1493 Size: 1697 Fragments arranged by size: 2109 1697 1087 876 292 226 152 146 52 AseI AT'TA_AT Cuts at: 160 1032 5191 160 Size: 872 4159 1606 Fragments arranged by size: 4159 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6637 AvaI C'yCGr_G Cuts at: 1870 1943 2111 3701 3962 1870 Size: 73 168 1590 261 4545 Fragments arranged by size: 4545 1590 261 168 73 BamHI G'GATC_C Cuts at: 3967 4334 3967 Size: 367 6270 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2115 3032 3872 3958 5413 Size: 325 217 627 328 917 840 86 1455 Cuts at: 5413 618 Size: 1842 Fragments arranged by size: 1842 1455 917 840 627 328 325 217 86 BanII G_rGCy'C Cuts at: 1391 3732 3956 1391 Size: 2341 224 4072 Fragments arranged by size: 4072 2341 224 BbeI G_GCGC'C Cuts at: 3036 3036 Size: 6637 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2263 2337 3111 928 Size: 971 364 74 774 4454 Fragments arranged by size: 4454 971 774 364 74 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4828 4862 1903 Size: 34 2891 34 3678 Fragments arranged by size: 3678 2891 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2973 4529 5739 2973 Size: 1556 1210 3871 Fragments arranged by size: 3871 1556 1210 BclI T'GATC_A Cuts at: 1548 2208 3588 1548 Size: 660 1380 4597 Fragments arranged by size: 4597 1380 660 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5248 136 Size: 107 122 71 631 605 3576 1525 Fragments arranged by size: 3576 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6632 6632 Size: 6637 BlpI GC'TnA_GC Cuts at: 4054 4054 Size: 6637 Bme1580I G_kGCm'C Cuts at: 1790 3421 4698 5854 1790 Size: 1631 1277 1156 2573 Fragments arranged by size: 2573 1631 1277 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6637 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2884 3734 4368 Size: 416 53 83 399 24 1467 850 634 Cuts at: 4368 5326 442 Size: 958 1753 Fragments arranged by size: 1753 1467 958 850 634 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6637 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2611 3729 2611 Size: 1118 5519 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2589 2606 5297 1239 Size: 1350 17 2691 2579 Fragments arranged by size: 2691 2579 1350 17 Bpu10I CC'TnA_GC Cuts at: 3651 3651 Size: 6637 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4762 5627 5925 4762 Size: 865 298 5474 Fragments arranged by size: 5474 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5300 882 Size: 617 3801 2219 Fragments arranged by size: 3801 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2263 4428 493 Size: 711 1059 2165 2702 Fragments arranged by size: 2702 2165 1059 711 BsaBI GATnn'nnATC Cuts at: 2719 6631 2719 Size: 3912 2725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3033 4444 4826 5461 Size: 53 83 186 936 1500 1411 382 635 Cuts at: 5461 275 Size: 1451 Fragments arranged by size: 1500 1451 1411 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2185 5070 5468 5742 5889 6300 2185 Size: 2885 398 274 147 411 2522 Fragments arranged by size: 2885 2522 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3077 3107 1178 Size: 30 165 30 1674 30 4708 Fragments arranged by size: 4708 1674 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2015 2322 3491 1186 Size: 829 307 1169 4332 Fragments arranged by size: 4332 1169 829 307 BseYI C'CCAG_C Cuts at: 3414 3745 5840 6336 3414 Size: 331 2095 496 3715 Fragments arranged by size: 3715 2095 496 331 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2418 1163 Size: 573 682 5382 Fragments arranged by size: 5382 682 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 2632 4087 4848 5876 1540 Size: 275 186 60 571 1455 761 1028 2301 Fragments arranged by size: 2301 1455 1028 761 571 275 186 60 BsiHKAI G_wGCw'C Cuts at: 2040 4698 4783 5854 2040 Size: 2658 85 1071 2823 Fragments arranged by size: 2823 2658 1071 85 BsmI GAATG_Cn' Cuts at: 2525 2727 4148 4241 2525 Size: 202 1421 93 4921 Fragments arranged by size: 4921 1421 202 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 3421 3732 3956 4698 4783 5854 Size: 399 250 1381 311 224 742 85 1071 Cuts at: 5854 1391 Size: 2174 Fragments arranged by size: 2174 1381 1071 742 399 311 250 224 85 BspEI T'CCGG_A Cuts at: 2185 6300 2185 Size: 4115 2522 BspHI T'CATG_A Cuts at: 3537 4526 6256 3537 Size: 989 1730 3918 Fragments arranged by size: 3918 1730 989 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3992 844 Size: 3148 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2023 4524 1394 Size: 429 200 2501 3507 Fragments arranged by size: 3507 2501 429 200 BsrDI GCAATG_nn' Cuts at: 65 1248 5132 5306 65 Size: 1183 3884 174 1396 Fragments arranged by size: 3884 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 2631 5281 5468 1700 Size: 110 250 571 2650 187 2869 Fragments arranged by size: 2869 2650 571 250 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6637 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6637 BssSI C'ACGA_G Cuts at: 4697 5709 4697 Size: 1012 5625 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6637 BstBI TT'CG_AA Cuts at: 5441 5441 Size: 6637 BstEII G'GTnAC_C Cuts at: 1184 3397 1184 Size: 2213 4424 BstXI CCAn_nnnn'nTGG Cuts at: 3883 3883 Size: 6637 BstYI r'GATC_y Cuts at: 1972 3693 3967 4334 4719 4736 6188 6274 6286 Size: 1721 274 367 385 17 1452 86 12 Cuts at: 6286 6632 1972 Size: 346 1977 Fragments arranged by size: 1977 1721 1452 385 367 346 274 86 17 12 BstZ17I GTA'TAC Cuts at: 3268 3268 Size: 6637 BtgI C'CryG_G Cuts at: 513 1065 2004 2736 3900 513 Size: 552 939 732 1164 3250 Fragments arranged by size: 3250 1164 939 732 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5898 Fragments arranged by size: 5898 683 56 BtsI GCAGTG_nn' Cuts at: 2812 4153 4947 4967 2812 Size: 1341 794 20 4482 Fragments arranged by size: 4482 1341 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6637 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3154 3189 529 Size: 35 2590 35 3977 Fragments arranged by size: 3977 2590 35 35 DraI TTT'AAA Cuts at: 3941 3941 Size: 6637 DraIII CAC_nnn'GTG Cuts at: 2396 2468 2396 Size: 72 6565 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5644 809 Size: 507 4328 1802 Fragments arranged by size: 4328 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 3885 4084 Size: 54 1425 50 275 18 228 1827 199 Cuts at: 4084 4973 6602 8 Size: 889 1629 43 Fragments arranged by size: 1827 1629 1425 889 275 228 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2058 4084 1537 Size: 275 246 2026 4090 Fragments arranged by size: 4090 2026 275 246 EarI CTCTTCn'nnn_ Cuts at: 1353 4567 1353 Size: 3214 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3380 3735 5224 5598 5744 387 Size: 1064 138 1791 355 1489 374 146 1280 Fragments arranged by size: 1791 1489 1280 1064 374 355 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2331 2589 2606 4700 5297 6084 6352 Size: 393 699 258 17 2094 597 787 268 Cuts at: 6352 1239 Size: 1524 Fragments arranged by size: 2094 1524 787 699 597 393 268 258 17 EcoNI CCTnn'n_nnAGG Cuts at: 3473 3473 Size: 6637 EcoO109I rG'GnC_Cy Cuts at: 2435 2435 Size: 6637 EcoRI G'AATT_C Cuts at: 3946 4324 3946 Size: 378 6259 FspI TGC'GCA Cuts at: 5143 5143 Size: 6637 HaeII r_GCGC'y Cuts at: 1678 2067 2920 3036 5784 1678 Size: 389 853 116 2748 2531 Fragments arranged by size: 2748 2531 853 389 116 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2167 2199 2314 2346 2605 Size: 32 135 32 595 32 115 32 259 Cuts at: 2605 2637 3077 3109 3714 3746 5278 5310 5352 Size: 32 440 32 605 32 1532 32 42 Cuts at: 5352 5384 1373 Size: 32 2626 Fragments arranged by size: 2626 1532 605 595 440 259 135 115 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3981 4227 669 Size: 988 281 2043 246 3079 Fragments arranged by size: 3079 2043 988 281 246 HindIII A'AGCT_T Cuts at: 748 2575 748 Size: 1827 4810 HpaI GTT'AAC Cuts at: 4227 4227 Size: 6637 KasI G'GCGC_C Cuts at: 3032 3032 Size: 6637 KpnI G_GTAC'C Cuts at: 2119 3962 2119 Size: 1843 4794 MfeI C'AATT_G Cuts at: 4236 4236 Size: 6637 MluI A'CGCG_T Cuts at: 6513 6513 Size: 6637 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3120 3378 5751 5935 1305 Size: 1815 258 2373 184 2007 Fragments arranged by size: 2373 2007 1815 258 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3887 10 Size: 54 1425 343 2055 2760 Fragments arranged by size: 2760 2055 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2154 3251 3878 4595 4954 5113 518 Size: 648 988 1097 627 717 359 159 2042 Fragments arranged by size: 2042 1097 988 717 648 627 359 159 MspA1I CmG'CkG Cuts at: 1606 3773 4053 4730 5878 6123 1606 Size: 2167 280 677 1148 245 2120 Fragments arranged by size: 2167 2120 1148 677 280 245 NaeI GCC'GGC Cuts at: 2062 2062 Size: 6637 NarI GG'CG_CC Cuts at: 3033 3033 Size: 6637 NcoI C'CATG_G Cuts at: 513 1065 2004 2736 513 Size: 552 939 732 4414 Fragments arranged by size: 4414 939 732 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6637 NgoMIV G'CCGG_C Cuts at: 2060 2060 Size: 6637 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6637 NotI GC'GGCC_GC Cuts at: 4084 4084 Size: 6637 NspI r_CATG'y Cuts at: 1132 1229 3995 5540 1132 Size: 97 2766 1545 2229 Fragments arranged by size: 2766 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6637 PciI A'CATG_T Cuts at: 5536 5536 Size: 6637 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6637 PfoI T'CCnGG_A Cuts at: 2562 2953 2562 Size: 391 6246 PmeI GTTT'AAAC Cuts at: 3941 3941 Size: 6637 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4706 4738 6219 6251 4706 Size: 32 1481 32 5092 Fragments arranged by size: 5092 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2435 2435 Size: 6637 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6637 PsiI TTA'TAA Cuts at: 4207 4207 Size: 6637 PstI C_TGCA'G Cuts at: 830 2914 3989 830 Size: 2084 1075 3478 Fragments arranged by size: 3478 2084 1075 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6637 PvuII CAG'CTG Cuts at: 3773 3773 Size: 6637 SalI G'TCGA_C Cuts at: 1936 3979 1936 Size: 2043 4594 SbfI CC_TGCA'GG Cuts at: 3989 3989 Size: 6637 ScaI AGT'ACT Cuts at: 4885 6597 4885 Size: 1712 4925 SexAI A'CCwGG_T Cuts at: 2701 2701 Size: 6637 SfcI C'TryA_G Cuts at: 826 1045 2788 2910 3768 3985 5120 5801 5992 Size: 219 1743 122 858 217 1135 681 191 Cuts at: 5992 826 Size: 1471 Fragments arranged by size: 1743 1471 1135 858 681 219 217 191 122 SfoI GGC'GCC Cuts at: 3034 3034 Size: 6637 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6637 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6637 SmaI CCC'GGG Cuts at: 1872 2113 3964 1872 Size: 241 1851 4545 Fragments arranged by size: 4545 1851 241 SmlI C'TyrA_G Cuts at: 820 1017 1943 4741 5642 5904 820 Size: 197 926 2798 901 262 1553 Fragments arranged by size: 2798 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4428 493 Size: 3935 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6637 SphI G_CATG'C Cuts at: 1132 3995 1132 Size: 2863 3774 SspI AAT'ATT Cuts at: 5 52 3062 4561 5 Size: 47 3010 1499 2081 Fragments arranged by size: 3010 2081 1499 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6637 StyI C'CwwG_G Cuts at: 513 1065 2004 2736 3358 4076 513 Size: 552 939 732 622 718 3074 Fragments arranged by size: 3074 939 732 718 622 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2166 2934 3793 4664 4681 4834 5019 Size: 1255 586 768 859 871 17 153 185 Cuts at: 5019 5464 325 Size: 445 1498 Fragments arranged by size: 1498 1255 871 859 768 586 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4883 6595 96 Size: 274 80 33 51 1443 2906 1712 138 Fragments arranged by size: 2906 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6597 6629 6597 Size: 32 6605 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6637 XbaI T'CTAG_A Cuts at: 3973 3973 Size: 6637 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3172 3796 3866 3172 Size: 624 70 5943 Fragments arranged by size: 5943 624 70 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6637 XmaI C'CCGG_G Cuts at: 1870 2111 3962 1870 Size: 241 1851 4545 Fragments arranged by size: 4545 1851 241 XmnI GAAnn'nnTTC Cuts at: 4766 4766 Size: 6637 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4445 276 Size: 53 83 186 936 2911 2468 Fragments arranged by size: 2911 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI