(Linear) (Six-base) MAPSORT of: KIEE5781.seq Check: 3569 from: 1 to: 1932 KIEE5781.orf With 182 enzymes: SgfI * December 5, 2014 13:10 .. AccI GT'mk_AC Cuts at: 0 983 1262 1932 Size: 983 279 670 Fragments arranged by size: 983 670 279 Acc65I G'GTAC_C Cuts at: 0 110 1932 Size: 110 1822 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 326 1932 Size: 326 1606 AfeI AGC'GCT Cuts at: 0 913 1932 Size: 913 1019 AleI CACnn'nnGTG Cuts at: 0 149 1873 1932 Size: 149 1724 59 Fragments arranged by size: 1724 149 59 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1891 1932 Size: 1891 41 AlwNI CAG_nnn'CTG Cuts at: 0 242 1367 1932 Size: 242 1125 565 Fragments arranged by size: 1125 565 242 ApoI r'AATT_y Cuts at: 0 364 416 562 854 1932 Size: 364 52 146 292 1078 Fragments arranged by size: 1078 364 292 146 52 AvaI C'yCGr_G Cuts at: 0 106 1696 1932 Size: 106 1590 236 Fragments arranged by size: 1590 236 106 BanI G'GyrC_C Cuts at: 0 110 1027 1867 1932 Size: 110 917 840 65 Fragments arranged by size: 917 840 110 65 BanII G_rGCy'C Cuts at: 0 1727 1932 Size: 1727 205 BbeI G_GCGC'C Cuts at: 0 1031 1932 Size: 1031 901 BbsI GAAGACnn'nnnn_ Cuts at: 0 258 332 1106 1932 Size: 258 74 774 826 Fragments arranged by size: 826 774 258 74 BciVI GTATCCnnnnn_n' Cuts at: 0 968 1932 Size: 968 964 BclI T'GATC_A Cuts at: 0 203 1583 1932 Size: 203 1380 349 Fragments arranged by size: 1380 349 203 Bme1580I G_kGCm'C Cuts at: 0 1416 1932 Size: 1416 516 BmrI ACTGGGnnnn_n' Cuts at: 0 879 1729 1932 Size: 879 850 203 Fragments arranged by size: 879 850 203 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 606 1724 1932 Size: 606 1118 208 Fragments arranged by size: 1118 606 208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 584 601 1932 Size: 584 17 1331 Fragments arranged by size: 1331 584 17 Bpu10I CC'TnA_GC Cuts at: 0 1646 1932 Size: 1646 286 BsaAI yAC'GTr Cuts at: 0 258 1932 Size: 258 1674 BsaBI GATnn'nnATC Cuts at: 0 714 1932 Size: 714 1218 BsaHI Gr'CG_yC Cuts at: 0 1028 1932 Size: 1028 904 BsaWI w'CCGG_w Cuts at: 0 180 1932 Size: 180 1752 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1072 1102 1932 Size: 1072 30 830 Fragments arranged by size: 1072 830 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 10 317 1486 1932 Size: 10 307 1169 446 Fragments arranged by size: 1169 446 307 10 BseYI C'CCAG_C Cuts at: 0 1409 1740 1932 Size: 1409 331 192 Fragments arranged by size: 1409 331 192 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 413 1932 Size: 413 1519 BsiEI CG_ry'CG Cuts at: 0 56 627 1932 Size: 56 571 1305 Fragments arranged by size: 1305 571 56 BsiHKAI G_wGCw'C Cuts at: 0 35 1932 Size: 35 1897 BsmI GAATG_Cn' Cuts at: 0 520 722 1932 Size: 520 202 1210 Fragments arranged by size: 1210 520 202 Bsp1286I G_dGCh'C Cuts at: 0 35 1416 1727 1932 Size: 35 1381 311 205 Fragments arranged by size: 1381 311 205 35 BspEI T'CCGG_A Cuts at: 0 180 1932 Size: 180 1752 BspHI T'CATG_A Cuts at: 0 1532 1932 Size: 1532 400 BsrBI CCG'CTC Cuts at: 0 18 1932 Size: 18 1914 BsrFI r'CCGG_y Cuts at: 0 55 626 1932 Size: 55 571 1306 Fragments arranged by size: 1306 571 55 BstEII G'GTnAC_C Cuts at: 0 1392 1932 Size: 1392 540 BstXI CCAn_nnnn'nTGG Cuts at: 0 1878 1932 Size: 1878 54 BstYI r'GATC_y Cuts at: 0 1688 1932 Size: 1688 244 BstZ17I GTA'TAC Cuts at: 0 1263 1932 Size: 1263 669 BtgI C'CryG_G Cuts at: 0 731 1895 1932 Size: 731 1164 37 Fragments arranged by size: 1164 731 37 BtsI GCAGTG_nn' Cuts at: 0 807 1932 Size: 807 1125 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1149 1184 1932 Size: 1149 35 748 Fragments arranged by size: 1149 748 35 DraIII CAC_nnn'GTG Cuts at: 0 391 463 1932 Size: 391 72 1469 Fragments arranged by size: 1469 391 72 EaeI y'GGCC_r Cuts at: 0 53 1880 1932 Size: 53 1827 52 Fragments arranged by size: 1827 53 52 EagI C'GGCC_G Cuts at: 0 53 1932 Size: 53 1879 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1375 1730 1932 Size: 1375 355 202 Fragments arranged by size: 1375 355 202 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 326 584 601 1932 Size: 326 258 17 1331 Fragments arranged by size: 1331 326 258 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 1468 1932 Size: 1468 464 EcoO109I rG'GnC_Cy Cuts at: 0 430 1932 Size: 430 1502 HaeII r_GCGC'y Cuts at: 0 62 915 1031 1932 Size: 62 853 116 901 Fragments arranged by size: 901 853 116 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 162 194 309 341 600 632 1072 1104 Size: 162 32 115 32 259 32 440 32 Cuts at: 1104 1709 1741 1932 Size: 605 32 191 Fragments arranged by size: 605 440 259 191 162 115 32 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 570 1932 Size: 570 1362 KasI G'GCGC_C Cuts at: 0 1027 1932 Size: 1027 905 KpnI G_GTAC'C Cuts at: 0 114 1932 Size: 114 1818 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1115 1373 1932 Size: 1115 258 559 Fragments arranged by size: 1115 559 258 MscI TGG'CCA Cuts at: 0 1882 1932 Size: 1882 50 MslI CAynn'nnrTG Cuts at: 0 149 1246 1873 1932 Size: 149 1097 627 59 Fragments arranged by size: 1097 627 149 59 MspA1I CmG'CkG Cuts at: 0 1768 1932 Size: 1768 164 NaeI GCC'GGC Cuts at: 0 57 1932 Size: 57 1875 NarI GG'CG_CC Cuts at: 0 1028 1932 Size: 1028 904 NcoI C'CATG_G Cuts at: 0 731 1932 Size: 731 1201 NgoMIV G'CCGG_C Cuts at: 0 55 1932 Size: 55 1877 PfoI T'CCnGG_A Cuts at: 0 557 948 1932 Size: 557 391 984 Fragments arranged by size: 984 557 391 PpuMI rG'GwC_Cy Cuts at: 0 430 1932 Size: 430 1502 PstI C_TGCA'G Cuts at: 0 909 1932 Size: 909 1023 PvuII CAG'CTG Cuts at: 0 1768 1932 Size: 1768 164 SexAI A'CCwGG_T Cuts at: 0 696 1932 Size: 696 1236 SfcI C'TryA_G Cuts at: 0 783 905 1763 1932 Size: 783 122 858 169 Fragments arranged by size: 858 783 169 122 SfoI GGC'GCC Cuts at: 0 1029 1932 Size: 1029 903 SmaI CCC'GGG Cuts at: 0 108 1932 Size: 108 1824 SspI AAT'ATT Cuts at: 0 1057 1932 Size: 1057 875 StyI C'CwwG_G Cuts at: 0 731 1353 1932 Size: 731 622 579 Fragments arranged by size: 731 622 579 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 161 929 1788 1932 Size: 161 768 859 144 Fragments arranged by size: 859 768 161 144 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1167 1791 1861 1932 Size: 1167 624 70 71 Fragments arranged by size: 1167 624 71 70 XmaI C'CCGG_G Cuts at: 0 106 1932 Size: 106 1826 Enzymes that do cut: AccI Acc65I AcuI AfeI AleI AlfI AlwNI ApoI AvaI BanI BanII BbeI BbsI BciVI BclI Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI BsrFI BstEII BstXI BstYI BstZ17I BtgI BtsI CspCI DraIII EaeI EagI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PfoI PpuMI PstI PvuII SexAI SfcI SfoI SmaI SspI StyI TaqII XcmI XmaI Enzymes that do not cut: AarI AatII AclI AflII AflIII AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BfrBI BglI BglII BlpI BmgBI BmtI BpuEI BsaI BsiWI BsmBI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI Bsu36I BtgZI ClaI DraI DrdI EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI MfeI MluI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI StuI SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI