(Circular) (Six-base) MAPSORT of: pFN21AB8531.seq Check: 5603 from: 1 to: 7594 pFN21AB8531 7594 bp With 182 enzymes: SgfI * June 19, 2019 18:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5404 278 Size: 53 83 186 936 3868 2468 Fragments arranged by size: 3868 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4937 1937 Size: 3000 4594 Acc65I G'GTAC_C Cuts at: 4915 4915 Size: 7594 AclI AA'CG_TT Cuts at: 1563 3823 5721 6094 1563 Size: 2260 1898 373 3063 Fragments arranged by size: 3063 2260 1898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2032 2088 2754 3371 3599 4367 5657 7041 Size: 400 56 666 617 228 768 1290 1384 Cuts at: 7041 7309 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1290 768 666 617 400 268 228 56 AfeI AGC'GCT Cuts at: 4480 4480 Size: 7594 AflII C'TTAA_G Cuts at: 820 1017 3623 820 Size: 197 2606 4791 Fragments arranged by size: 4791 2606 197 AflIII A'CryG_T Cuts at: 1203 2575 6493 7470 1203 Size: 1372 3918 977 1327 Fragments arranged by size: 3918 1372 1327 977 AgeI A'CCGG_T Cuts at: 6425 6425 Size: 7594 AhdI GACnn_n'nnGTC Cuts at: 6323 6323 Size: 7594 AleI CACnn'nnGTG Cuts at: 1166 2120 4535 4815 1166 Size: 954 2415 280 3945 Fragments arranged by size: 3945 2415 954 280 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7594 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4101 4133 4101 Size: 32 7562 AlwNI CAG_nnn'CTG Cuts at: 4423 5337 6909 4423 Size: 914 1572 5108 Fragments arranged by size: 5108 1572 914 ApaLI G'TGCA_C Cuts at: 2506 4538 5651 6807 2506 Size: 2032 1113 1156 3293 Fragments arranged by size: 3293 2032 1156 1113 ApoI r'AATT_y Cuts at: 1493 2377 2384 3238 4903 5129 5281 7390 1493 Size: 884 7 854 1665 226 152 2109 1697 Fragments arranged by size: 2109 1697 1665 884 854 226 152 7 AseI AT'TA_AT Cuts at: 160 1032 2634 6148 160 Size: 872 1602 3514 1606 Fragments arranged by size: 3514 1606 1602 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7594 AvaI C'yCGr_G Cuts at: 1870 1943 4781 4919 1870 Size: 73 2838 138 4545 Fragments arranged by size: 4545 2838 138 73 BamHI G'GATC_C Cuts at: 4924 5291 4924 Size: 367 7227 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3124 4915 6370 618 Size: 325 217 627 1337 1791 1455 1842 Fragments arranged by size: 1842 1791 1455 1337 627 325 217 BanII G_rGCy'C Cuts at: 1391 3099 4913 1391 Size: 1708 1814 4072 Fragments arranged by size: 4072 1814 1708 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2118 2282 2481 3359 3540 928 Size: 971 219 164 199 878 181 4982 Fragments arranged by size: 4982 971 878 219 199 181 164 BbvCI CC'TCA_GC Cuts at: 2811 2811 Size: 7594 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5785 5819 1903 Size: 34 3848 34 3678 Fragments arranged by size: 3848 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2539 5486 6696 2539 Size: 2947 1210 3437 Fragments arranged by size: 3437 2947 1210 BclI T'GATC_A Cuts at: 1548 3511 1548 Size: 1963 5631 BfrBI ATG'CAT Cuts at: 2144 4667 2144 Size: 2523 5071 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6205 136 Size: 107 122 71 631 605 4533 1525 Fragments arranged by size: 4533 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4567 7589 4567 Size: 3022 4572 BlpI GC'TnA_GC Cuts at: 3420 3609 5011 3420 Size: 189 1402 6003 Fragments arranged by size: 6003 1402 189 Bme1580I G_kGCm'C Cuts at: 1790 2510 4542 5655 6811 1790 Size: 720 2032 1113 1156 2573 Fragments arranged by size: 2573 2032 1156 1113 720 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7594 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5325 6283 442 Size: 416 53 83 399 24 3908 958 1753 Fragments arranged by size: 3908 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7594 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2748 6254 1239 Size: 1509 3506 2579 Fragments arranged by size: 3506 2579 1509 Bpu10I CC'TnA_GC Cuts at: 2811 2811 Size: 7594 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2160 5719 6584 6882 2160 Size: 3559 865 298 2872 Fragments arranged by size: 3559 2872 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6257 882 Size: 617 4758 2219 Fragments arranged by size: 4758 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4556 5385 493 Size: 711 3352 829 2702 Fragments arranged by size: 3352 2702 829 711 BsaBI GATnn'nnATC Cuts at: 7588 7588 Size: 7594 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5401 5783 6418 275 Size: 53 83 186 936 3868 382 635 1451 Fragments arranged by size: 3868 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4469 6027 6425 6699 6846 7257 4469 Size: 1558 398 274 147 411 4806 Fragments arranged by size: 4806 1558 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2605 2635 1178 Size: 30 165 30 1202 30 6137 Fragments arranged by size: 6137 1202 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3389 4845 4893 1186 Size: 2203 1456 48 3887 Fragments arranged by size: 3887 2203 1456 48 BseYI C'CCAG_C Cuts at: 6797 7293 6797 Size: 496 7098 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4740 1163 Size: 573 3004 4017 Fragments arranged by size: 4017 3004 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2121 5044 5805 6833 1540 Size: 275 186 120 2923 761 1028 2301 Fragments arranged by size: 2923 2301 1028 761 275 186 120 BsiHKAI G_wGCw'C Cuts at: 2510 3099 4033 4065 4542 5655 5740 6811 2510 Size: 589 934 32 477 1113 85 1071 3293 Fragments arranged by size: 3293 1113 1071 934 589 477 85 32 BsmI GAATG_Cn' Cuts at: 4750 5105 5198 4750 Size: 355 93 7146 Fragments arranged by size: 7146 355 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3113 3113 Size: 7594 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2510 3099 4033 4065 4542 4913 5655 Size: 399 720 589 934 32 477 371 742 Cuts at: 5655 5740 6811 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 934 742 720 589 477 399 371 85 32 BspEI T'CCGG_A Cuts at: 7257 7257 Size: 7594 BspHI T'CATG_A Cuts at: 2302 2470 5483 7213 2302 Size: 168 3013 1730 2683 Fragments arranged by size: 3013 2683 1730 168 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4407 4949 844 Size: 3563 542 3489 Fragments arranged by size: 3563 3489 542 BsrBI CCG'CTC Cuts at: 1394 1823 5481 1394 Size: 429 3658 3507 Fragments arranged by size: 3658 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6089 6263 65 Size: 1183 4841 174 1396 Fragments arranged by size: 4841 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6238 6425 1700 Size: 110 4428 187 2869 Fragments arranged by size: 4428 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7594 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7594 BssSI C'ACGA_G Cuts at: 4671 5654 6666 4671 Size: 983 1012 5599 Fragments arranged by size: 5599 1012 983 BstAPI GCAn_nnn'nTGC Cuts at: 794 4763 794 Size: 3969 3625 BstBI TT'CG_AA Cuts at: 6398 6398 Size: 7594 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7594 BstYI r'GATC_y Cuts at: 1972 3060 4116 4567 4924 5291 5676 5693 7145 Size: 1088 1056 451 357 367 385 17 1452 Cuts at: 7145 7231 7243 7589 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1088 1056 451 385 367 357 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3735 3735 Size: 7594 BtgI C'CryG_G Cuts at: 513 1065 2004 2077 2367 3813 513 Size: 552 939 73 290 1446 4294 Fragments arranged by size: 4294 1446 939 552 290 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2077 487 Size: 683 56 851 6004 Fragments arranged by size: 6004 851 683 56 BtsI GCAGTG_nn' Cuts at: 4872 5110 5904 5924 4872 Size: 238 794 20 6542 Fragments arranged by size: 6542 794 238 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7594 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7559 DraI TTT'AAA Cuts at: 4898 4898 Size: 7594 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6601 809 Size: 507 5285 1802 Fragments arranged by size: 5285 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2959 4737 Size: 54 1425 50 275 18 186 943 1778 Cuts at: 4737 5041 5930 7559 8 Size: 304 889 1629 43 Fragments arranged by size: 1778 1629 1425 943 889 304 275 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5041 1537 Size: 275 3229 4090 Fragments arranged by size: 4090 3229 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3753 4356 4738 5524 1353 Size: 2400 603 382 786 3423 Fragments arranged by size: 3423 2400 786 603 382 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 6181 6555 6701 387 Size: 1064 138 435 4157 374 146 1280 Fragments arranged by size: 4157 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 3097 3097 Size: 7594 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2032 2088 2748 2754 3371 3599 4367 Size: 393 400 56 660 6 617 228 768 Cuts at: 4367 5657 6254 7041 7309 1239 Size: 1290 597 787 268 1524 Fragments arranged by size: 1524 1290 787 768 660 617 597 400 393 268 228 56 6 EcoO109I rG'GnC_Cy Cuts at: 2434 3484 4420 2434 Size: 1050 936 5608 Fragments arranged by size: 5608 1050 936 EcoRI G'AATT_C Cuts at: 4903 5281 4903 Size: 378 7216 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2240 4081 4205 2240 Size: 1841 124 5629 Fragments arranged by size: 5629 1841 124 FspI TGC'GCA Cuts at: 4403 6100 4403 Size: 1697 5897 HaeII r_GCGC'y Cuts at: 1678 4482 6741 1678 Size: 2804 2259 2531 Fragments arranged by size: 2804 2531 2259 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3503 3535 6235 6267 6309 Size: 32 135 32 1931 32 2700 32 42 Cuts at: 6309 6341 1373 Size: 32 2626 Fragments arranged by size: 2700 2626 1931 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3009 3335 4938 5184 669 Size: 988 281 1071 326 1603 246 3079 Fragments arranged by size: 3079 1603 1071 988 326 281 246 HindIII A'AGCT_T Cuts at: 748 3168 4629 748 Size: 2420 1461 3713 Fragments arranged by size: 3713 2420 1461 HpaI GTT'AAC Cuts at: 3009 5184 3009 Size: 2175 5419 KpnI G_GTAC'C Cuts at: 4919 4919 Size: 7594 MfeI C'AATT_G Cuts at: 3222 5193 3222 Size: 1971 5623 MluI A'CGCG_T Cuts at: 7470 7470 Size: 7594 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2592 3345 3963 4572 4829 6708 6892 1305 Size: 1287 753 618 609 257 1879 184 2007 Fragments arranged by size: 2007 1879 1287 753 618 609 257 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2961 4739 10 Size: 54 1425 343 1129 1778 2865 Fragments arranged by size: 2865 1778 1425 1129 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2033 2120 4535 4815 5552 5911 6070 Size: 648 867 87 2415 280 737 359 159 Cuts at: 6070 518 Size: 2042 Fragments arranged by size: 2415 2042 867 737 648 359 280 159 87 MspA1I CmG'CkG Cuts at: 1606 2079 3659 4575 4616 5010 5687 6835 7080 Size: 473 1580 916 41 394 677 1148 245 Cuts at: 7080 1606 Size: 2120 Fragments arranged by size: 2120 1580 1148 916 677 473 394 245 41 NcoI C'CATG_G Cuts at: 513 1065 2004 2367 3813 513 Size: 552 939 363 1446 4294 Fragments arranged by size: 4294 1446 939 552 363 NdeI CA'TA_TG Cuts at: 387 2254 2824 387 Size: 1867 570 5157 Fragments arranged by size: 5157 1867 570 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7594 NotI GC'GGCC_GC Cuts at: 5041 5041 Size: 7594 NsiI A_TGCA'T Cuts at: 2146 4669 2146 Size: 2523 5071 NspI r_CATG'y Cuts at: 1132 1229 2579 3900 4952 6497 1132 Size: 97 1350 1321 1052 1545 2229 Fragments arranged by size: 2229 1545 1350 1321 1052 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7594 PciI A'CATG_T Cuts at: 2575 6493 2575 Size: 3918 3676 PflMI CCAn_nnn'nTGG Cuts at: 1108 4051 4861 1108 Size: 2943 810 3841 Fragments arranged by size: 3841 2943 810 PmeI GTTT'AAAC Cuts at: 4898 4898 Size: 7594 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5663 5695 7176 7208 5663 Size: 32 1481 32 6049 Fragments arranged by size: 6049 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2434 4420 2434 Size: 1986 5608 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7594 PsiI TTA'TAA Cuts at: 5164 5164 Size: 7594 PstI C_TGCA'G Cuts at: 830 3016 4946 830 Size: 2186 1930 3478 Fragments arranged by size: 3478 2186 1930 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7594 PvuII CAG'CTG Cuts at: 3659 4616 3659 Size: 957 6637 SacI G_AGCT'C Cuts at: 3099 3099 Size: 7594 SacII CC_GC'GG Cuts at: 2080 2080 Size: 7594 SalI G'TCGA_C Cuts at: 1936 4936 1936 Size: 3000 4594 SbfI CC_TGCA'GG Cuts at: 4946 4946 Size: 7594 ScaI AGT'ACT Cuts at: 5842 7554 5842 Size: 1712 5882 SfcI C'TryA_G Cuts at: 826 1045 2884 3012 4611 4942 6077 6758 6949 Size: 219 1839 128 1599 331 1135 681 191 Cuts at: 6949 826 Size: 1471 Fragments arranged by size: 1839 1599 1471 1135 681 331 219 191 128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7594 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7594 SmaI CCC'GGG Cuts at: 1872 4783 4921 1872 Size: 2911 138 4545 Fragments arranged by size: 4545 2911 138 SmlI C'TyrA_G Cuts at: 820 1017 1943 2175 3623 5698 6599 6861 820 Size: 197 926 232 1448 2075 901 262 1553 Fragments arranged by size: 2075 1553 1448 926 901 262 232 197 SnaBI TAC'GTA Cuts at: 493 5385 493 Size: 4892 2702 SpeI A'CTAG_T Cuts at: 152 4015 152 Size: 3863 3731 SphI G_CATG'C Cuts at: 1132 4952 1132 Size: 3820 3774 SspI AAT'ATT Cuts at: 5 52 2247 5518 5 Size: 47 2195 3271 2081 Fragments arranged by size: 3271 2195 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7594 StyI C'CwwG_G Cuts at: 513 1065 2004 2367 3813 5033 513 Size: 552 939 363 1446 1220 3074 Fragments arranged by size: 3074 1446 1220 939 552 363 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4827 5621 5638 5791 5976 6421 325 Size: 1255 3247 794 17 153 185 445 1498 Fragments arranged by size: 3247 1498 1255 794 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2088 2221 2819 Size: 274 80 33 51 1443 111 133 598 Cuts at: 2819 5840 7552 96 Size: 3021 1712 138 Fragments arranged by size: 3021 1712 1443 598 274 138 133 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3693 3725 7554 7586 3693 Size: 32 3829 32 3701 Fragments arranged by size: 3829 3701 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2117 1316 Size: 801 6793 XbaI T'CTAG_A Cuts at: 4930 4930 Size: 7594 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 7594 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7594 XmaI C'CCGG_G Cuts at: 1870 4781 4919 1870 Size: 2911 138 4545 Fragments arranged by size: 4545 2911 138 XmnI GAAnn'nnTTC Cuts at: 3951 5723 3951 Size: 1772 5822 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5402 276 Size: 53 83 186 936 3868 2468 Fragments arranged by size: 3868 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI