(Linear) (Six-base) MAPSORT of: KIBB8531.seq Check: 5816 from: 1 to: 2889 KIBB8531.orf With 182 enzymes: SgfI * June 19, 2019 14:13 .. AclI AA'CG_TT Cuts at: 0 1818 2889 Size: 1818 1071 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 27 83 749 1366 1594 2362 2889 Size: 27 56 666 617 228 768 527 Fragments arranged by size: 768 666 617 527 228 56 27 AfeI AGC'GCT Cuts at: 0 2475 2889 Size: 2475 414 AflII C'TTAA_G Cuts at: 0 1618 2889 Size: 1618 1271 AflIII A'CryG_T Cuts at: 0 570 2889 Size: 570 2319 AleI CACnn'nnGTG Cuts at: 0 115 2530 2810 2889 Size: 115 2415 280 79 Fragments arranged by size: 2415 280 115 79 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2096 2128 2889 Size: 2096 32 761 Fragments arranged by size: 2096 761 32 AlwNI CAG_nnn'CTG Cuts at: 0 2418 2889 Size: 2418 471 ApaLI G'TGCA_C Cuts at: 0 501 2533 2889 Size: 501 2032 356 Fragments arranged by size: 2032 501 356 ApoI r'AATT_y Cuts at: 0 372 379 1233 2889 Size: 372 7 854 1656 Fragments arranged by size: 1656 854 372 7 AseI AT'TA_AT Cuts at: 0 629 2889 Size: 629 2260 AvaI C'yCGr_G Cuts at: 0 2776 2889 Size: 2776 113 BanI G'GyrC_C Cuts at: 0 1119 2889 Size: 1119 1770 BanII G_rGCy'C Cuts at: 0 1094 2889 Size: 1094 1795 BbsI GAAGACnn'nnnn_ Cuts at: 0 113 277 476 1354 1535 2889 Size: 113 164 199 878 181 1354 Fragments arranged by size: 1354 878 199 181 164 113 BbvCI CC'TCA_GC Cuts at: 0 806 2889 Size: 806 2083 BciVI GTATCCnnnnn_n' Cuts at: 0 534 2889 Size: 534 2355 BclI T'GATC_A Cuts at: 0 1506 2889 Size: 1506 1383 BfrBI ATG'CAT Cuts at: 0 139 2662 2889 Size: 139 2523 227 Fragments arranged by size: 2523 227 139 BglII A'GATC_T Cuts at: 0 2562 2889 Size: 2562 327 BlpI GC'TnA_GC Cuts at: 0 1415 1604 2889 Size: 1415 189 1285 Fragments arranged by size: 1415 1285 189 Bme1580I G_kGCm'C Cuts at: 0 505 2537 2889 Size: 505 2032 352 Fragments arranged by size: 2032 505 352 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 743 2889 Size: 743 2146 Bpu10I CC'TnA_GC Cuts at: 0 806 2889 Size: 806 2083 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 155 2889 Size: 155 2734 BsaAI yAC'GTr Cuts at: 0 2551 2889 Size: 2551 338 BsaWI w'CCGG_w Cuts at: 0 2464 2889 Size: 2464 425 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 600 630 2889 Size: 600 30 2259 Fragments arranged by size: 2259 600 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1384 2840 2888 2889 Size: 1384 1456 48 1 Fragments arranged by size: 1456 1384 48 1 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2735 2889 Size: 2735 154 BsiEI CG_ry'CG Cuts at: 0 116 2889 Size: 116 2773 BsiHKAI G_wGCw'C Cuts at: 0 505 1094 2028 2060 2537 2889 Size: 505 589 934 32 477 352 Fragments arranged by size: 934 589 505 477 352 32 BsmI GAATG_Cn' Cuts at: 0 2745 2889 Size: 2745 144 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1108 2889 Size: 1108 1781 Bsp1286I G_dGCh'C Cuts at: 0 505 1094 2028 2060 2537 2889 Size: 505 589 934 32 477 352 Fragments arranged by size: 934 589 505 477 352 32 BspHI T'CATG_A Cuts at: 0 297 465 2889 Size: 297 168 2424 Fragments arranged by size: 2424 297 168 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2402 2889 Size: 2402 487 BssSI C'ACGA_G Cuts at: 0 2666 2889 Size: 2666 223 BstAPI GCAn_nnn'nTGC Cuts at: 0 2758 2889 Size: 2758 131 BstYI r'GATC_y Cuts at: 0 1055 2111 2562 2889 Size: 1055 1056 451 327 Fragments arranged by size: 1056 1055 451 327 Bsu36I CC'TnA_GG Cuts at: 0 1730 2889 Size: 1730 1159 BtgI C'CryG_G Cuts at: 0 72 362 1808 2889 Size: 72 290 1446 1081 Fragments arranged by size: 1446 1081 290 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 72 2889 Size: 72 2817 BtsI GCAGTG_nn' Cuts at: 0 2867 2889 Size: 2867 22 EaeI y'GGCC_r Cuts at: 0 11 954 2732 2889 Size: 11 943 1778 157 Fragments arranged by size: 1778 943 157 11 EarI CTCTTCn'nnn_ Cuts at: 0 1748 2351 2733 2889 Size: 1748 603 382 156 Fragments arranged by size: 1748 603 382 156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 2889 Size: 19 2870 EcoICRI GAG'CTC Cuts at: 0 1092 2889 Size: 1092 1797 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 27 83 743 749 1366 1594 2362 2889 Size: 27 56 660 6 617 228 768 527 Fragments arranged by size: 768 660 617 527 228 56 27 6 EcoO109I rG'GnC_Cy Cuts at: 0 429 1479 2415 2889 Size: 429 1050 936 474 Fragments arranged by size: 1050 936 474 429 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 235 2076 2200 2889 Size: 235 1841 124 689 Fragments arranged by size: 1841 689 235 124 FspI TGC'GCA Cuts at: 0 2398 2889 Size: 2398 491 HaeII r_GCGC'y Cuts at: 0 2477 2889 Size: 2477 412 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1498 1530 2889 Size: 1498 32 1359 Fragments arranged by size: 1498 1359 32 HincII GTy'rAC Cuts at: 0 1004 1330 2889 Size: 1004 326 1559 Fragments arranged by size: 1559 1004 326 HindIII A'AGCT_T Cuts at: 0 1163 2624 2889 Size: 1163 1461 265 Fragments arranged by size: 1461 1163 265 HpaI GTT'AAC Cuts at: 0 1004 2889 Size: 1004 1885 MfeI C'AATT_G Cuts at: 0 1217 2889 Size: 1217 1672 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 587 1340 1958 2567 2824 2889 Size: 587 753 618 609 257 65 Fragments arranged by size: 753 618 609 587 257 65 MscI TGG'CCA Cuts at: 0 956 2734 2889 Size: 956 1778 155 Fragments arranged by size: 1778 956 155 MslI CAynn'nnrTG Cuts at: 0 28 115 2530 2810 2889 Size: 28 87 2415 280 79 Fragments arranged by size: 2415 280 87 79 28 MspA1I CmG'CkG Cuts at: 0 74 1654 2570 2611 2889 Size: 74 1580 916 41 278 Fragments arranged by size: 1580 916 278 74 41 NcoI C'CATG_G Cuts at: 0 362 1808 2889 Size: 362 1446 1081 Fragments arranged by size: 1446 1081 362 NdeI CA'TA_TG Cuts at: 0 249 819 2889 Size: 249 570 2070 Fragments arranged by size: 2070 570 249 NsiI A_TGCA'T Cuts at: 0 141 2664 2889 Size: 141 2523 225 Fragments arranged by size: 2523 225 141 NspI r_CATG'y Cuts at: 0 574 1895 2889 Size: 574 1321 994 Fragments arranged by size: 1321 994 574 PciI A'CATG_T Cuts at: 0 570 2889 Size: 570 2319 PflMI CCAn_nnn'nTGG Cuts at: 0 2046 2856 2889 Size: 2046 810 33 Fragments arranged by size: 2046 810 33 PpuMI rG'GwC_Cy Cuts at: 0 429 2415 2889 Size: 429 1986 474 Fragments arranged by size: 1986 474 429 PstI C_TGCA'G Cuts at: 0 1011 2889 Size: 1011 1878 PvuII CAG'CTG Cuts at: 0 1654 2611 2889 Size: 1654 957 278 Fragments arranged by size: 1654 957 278 SacI G_AGCT'C Cuts at: 0 1094 2889 Size: 1094 1795 SacII CC_GC'GG Cuts at: 0 75 2889 Size: 75 2814 SfcI C'TryA_G Cuts at: 0 879 1007 2606 2889 Size: 879 128 1599 283 Fragments arranged by size: 1599 879 283 128 SmaI CCC'GGG Cuts at: 0 2778 2889 Size: 2778 111 SmlI C'TyrA_G Cuts at: 0 170 1618 2889 Size: 170 1448 1271 Fragments arranged by size: 1448 1271 170 SpeI A'CTAG_T Cuts at: 0 2010 2889 Size: 2010 879 SspI AAT'ATT Cuts at: 0 242 2889 Size: 242 2647 StyI C'CwwG_G Cuts at: 0 362 1808 2889 Size: 362 1446 1081 Fragments arranged by size: 1446 1081 362 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2822 2889 Size: 2822 67 TatI w'GTAC_w Cuts at: 0 83 216 814 2889 Size: 83 133 598 2075 Fragments arranged by size: 2075 598 133 83 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1688 1720 2889 Size: 1688 32 1169 Fragments arranged by size: 1688 1169 32 Tth111I GACn'n_nGTC Cuts at: 0 112 2889 Size: 112 2777 XmaI C'CCGG_G Cuts at: 0 2776 2889 Size: 2776 113 XmnI GAAnn'nnTTC Cuts at: 0 1946 2889 Size: 1946 943 Enzymes that do cut: AclI AcuI AfeI AflII AflIII AleI AloI AlwNI ApaLI ApoI AseI AvaI BanI BanII BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BssSI BstAPI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PciI PflMI PpuMI PstI PvuII SacI SacII SfcI SmaI SmlI SpeI SspI StyI TaqII TatI TstI Tth111I XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AgeI AhdI AlfI ApaI AscI AsiSI AvrII BaeI BamHI BbeI BcgI BglI BmgBI BmrI BmtI BplI BsaI BsaBI BsaHI BseYI BsiWI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FseI FspAI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI StuI SwaI XbaI XcmI XhoI ZraI