################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BmyI BpmI BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcyI 184 GGCGCC G[GA]^CG[CT]C AlwI 278 GGATC GGATC(4/5) AlwNI 77 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 231 CTCGAG C^[CT]CG[GA]G AvaII 327 GGTCC G^G[AT]CC BbeI 184 GGCGCC GGCGC^C BglI 79 GCCTCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 431 GCTCAGC GC^T[ACGT]AGC Bpu1102I 431 GCTCAGC GC^T[ACGT]AGC BsaHI 184 GGCGCC G[GA]^CG[CT]C BsaI 545 GAGACC GGTCTC(1/5) BsgI 384 GTGCAG GTGCAG(16/14) BsmI 152 GCATTC GAATGC(1/-1) BsoBI 231 CTCGAG C^[CT]CG[GA]G BsrBI 300 GAGCGG CCGCTC(-3/-3) BstYI 278 GGATCT [GA]^GATC[CT] CelII 431 GCTCAGC GC^T[ACGT]AGC CfoI 185 GCGC GCG^C Cfr13I 327 GGTCC G^G[ACGT]CC FauI 148 GCGGG CCCGC(4/6) HaeII 184 GGCGCC [GA]GCGC^[CT] HaeIII 241 GGCC GG^CC HapII 123 CCGG C^CGG HhaI 185 GCGC GCG^C Hin1I 184 GGCGCC G[GA]^CG[CT]C HinP1I 185 GCGC G^CGC HpaII 123 CCGG C^CGG HphI 28 TCACC GGTGA(8/7) KasI 184 GGCGCC G^GCGCC MaeII 422 ACGT A^CGT MflI 278 GGATCT [GA]^GATC[CT] MseI 488 TTAA T^TAA MspI 123 CCGG C^CGG NarI 184 GGCGCC GG^CGCC NlaIII 285 CATG CATG^ PaeR7I 231 CTCGAG C^TCGAG Sau96I 327 GGTCC G^G[ACGT]CC SfoI 184 GGCGCC GGC^GCC SmlI 231 CTCGAG C^T[CT][GA]AG SspI 225 AATATT AAT^ATT TfiI 99 GAATC G^A[AT]TC Tru9I 488 TTAA T^TAA XhoI 231 CTCGAG C^TCGAG XhoII 278 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 378 CGCG CG^CG 391 CGCG BanI 91 GGCACC G^G[CT][GA]CC 184 GGCGCC BsaJI 267 CCTTGG C^C[ACGT][ACGT]GG 437 CCTTGG BstF5I 137 GGATG GGATG(2/0) 333 GGATG BstUI 378 CGCG CG^CG 391 CGCG DpnII 279 GATC ^GATC 366 GATC EcoT14I 267 CCTTGG C^C[AT][AT]GG 437 CCTTGG FokI 137 GGATG GGATG(9/13) 333 GGATG HgaI 7 GACGC GACGC(5/10) 389 GACGC MboI 279 GATC ^GATC 366 GATC MvnI 378 CGCG CG^CG 391 CGCG MwoI 79 GCCTCTGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 427 GCCTGCTCAGC NdeII 279 GATC ^GATC 366 GATC NlaIV 91 GGCACC GG[ACGT]^[ACGT]CC 184 GGCGCC PleI 61 GACTC GAGTC(4/5) 190 GAGTC Sau3AI 279 GATC ^GATC 366 GATC StyI 267 CCTTGG C^C[AT][AT]GG 437 CCTTGG TaqI 232 TCGA T^CGA 347 TCGA TthHB8I 232 TCGA T^CGA 347 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AluI BbvI BsmAI BsrI BssKI BstNI Cac8I DdeI EcoRII Fnu4HI HinfI ItaI MaeIII MboII MnlI MvaI ScrFI SfaNI TseI Tsp45I Tsp509I TspRI