Restriction map for
pFN21AE5250
(Circular) (Six-base) MAPSORT of: pFN21AE5250.seq Check: 3109 from: 1 to: 5287
pFN21AE5250 5287 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3097 278
Size: 53 83 186 936 1561 2468
Fragments arranged by size:
2468 1561 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2630 1937
Size: 693 4594
Acc65I G'GTAC_C
Cuts at: 2608 2608
Size: 5287
AclI AA'CG_TT
Cuts at: 1563 3414 3787 1563
Size: 1851 373 3063
Fragments arranged by size:
3063 1851 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2251 2426 3350 4734 5002 1632
Size: 619 175 924 1384 268 1917
Fragments arranged by size:
1917 1384 924 619 268 175
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5090
AflIII A'CryG_T
Cuts at: 1203 4186 5163 1203
Size: 2983 977 1327
Fragments arranged by size:
2983 1327 977
AgeI A'CCGG_T
Cuts at: 4118 4118
Size: 5287
AhdI GACnn_n'nnGTC
Cuts at: 4016 4016
Size: 5287
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5287
AlwNI CAG_nnn'CTG
Cuts at: 2087 3030 4602 2087
Size: 943 1572 2772
Fragments arranged by size:
2772 1572 943
ApaLI G'TGCA_C
Cuts at: 3344 4500 3344
Size: 1156 4131
ApoI r'AATT_y
Cuts at: 1493 2596 2822 2974 5083 1493
Size: 1103 226 152 2109 1697
Fragments arranged by size:
2109 1697 1103 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3841 160
Size: 872 2809 1606
Fragments arranged by size:
2809 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5287
AvaI C'yCGr_G
Cuts at: 1870 1943 2236 2612 1870
Size: 73 293 376 4545
Fragments arranged by size:
4545 376 293 73
BamHI G'GATC_C
Cuts at: 2617 2984 2617
Size: 367 4920
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 2189 2608 4063 618
Size: 325 217 627 309 93 419 1455 1842
Fragments arranged by size:
1842 1455 627 419 325 309 217 93
BanII G_rGCy'C
Cuts at: 1391 2606 1391
Size: 1215 4072
BbeI G_GCGC'C
Cuts at: 2193 2193
Size: 5287
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3478 3512 1903
Size: 34 50 34 1457 34 3678
Fragments arranged by size:
3678 1457 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3179 4389 3179
Size: 1210 4077
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5287
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2090 3898 136
Size: 107 122 71 631 605 418 1808 1525
Fragments arranged by size:
1808 1525 631 605 418 122 107 71
BglII A'GATC_T
Cuts at: 5282 5282
Size: 5287
BlpI GC'TnA_GC
Cuts at: 2437 2704 2437
Size: 267 5020
Bme1580I G_kGCm'C
Cuts at: 1790 3348 4504 1790
Size: 1558 1156 2573
Fragments arranged by size:
2573 1558 1156
BmgBI CAC'GTC
Cuts at: 1318 2428 1318
Size: 1110 4177
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2269 3018 3976
Size: 416 53 83 399 24 852 749 958
Cuts at: 3976 442
Size: 1753
Fragments arranged by size:
1753 958 852 749 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3947 1239
Size: 2708 2579
Bpu10I CC'TnA_GC
Cuts at: 2021 2021
Size: 5287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3412 4277 4575 3412
Size: 865 298 4124
Fragments arranged by size:
4124 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2544 3950 882
Size: 617 1045 1406 2219
Fragments arranged by size:
2219 1406 1045 617
BsaAI yAC'GTr
Cuts at: 493 1204 3078 493
Size: 711 1874 2702
Fragments arranged by size:
2702 1874 711
BsaBI GATnn'nnATC
Cuts at: 5281 5281
Size: 5287
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2190 3094 3476 4111
Size: 53 83 186 936 657 904 382 635
Cuts at: 4111 275
Size: 1451
Fragments arranged by size:
1451 936 904 657 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3720 4118 4392 4539 4950 3720
Size: 398 274 147 411 4057
Fragments arranged by size:
4057 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5062
Fragments arranged by size:
5062 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5287
BseYI C'CCAG_C
Cuts at: 4490 4986 4490
Size: 496 4791
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2410 1163
Size: 573 674 4040
Fragments arranged by size:
4040 674 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2737 3498 4526 1540
Size: 275 186 736 761 1028 2301
Fragments arranged by size:
2301 1028 761 736 275 186
BsiHKAI G_wGCw'C
Cuts at: 3348 3433 4504 3348
Size: 85 1071 4131
Fragments arranged by size:
4131 1071 85
BsmI GAATG_Cn'
Cuts at: 2157 2798 2891 2157
Size: 641 93 4553
Fragments arranged by size:
4553 641 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2606 3348 3433 4504 1391
Size: 399 816 742 85 1071 2174
Fragments arranged by size:
2174 1071 816 742 399 85
BspEI T'CCGG_A
Cuts at: 4950 4950
Size: 5287
BspHI T'CATG_A
Cuts at: 3176 4906 3176
Size: 1730 3557
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2642 844
Size: 1798 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2307 3174 1394
Size: 429 484 867 3507
Fragments arranged by size:
3507 867 484 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3782 3956 65
Size: 1183 2534 174 1396
Fragments arranged by size:
2534 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3931 4118 1700
Size: 110 2121 187 2869
Fragments arranged by size:
2869 2121 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5287
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5287
BssSI C'ACGA_G
Cuts at: 3347 4359 3347
Size: 1012 4275
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5287
BstBI TT'CG_AA
Cuts at: 4091 4091
Size: 5287
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5287
BstYI r'GATC_y
Cuts at: 1972 2283 2617 2984 3369 3386 4838 4924 4936
Size: 311 334 367 385 17 1452 86 12
Cuts at: 4936 5282 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 334 311 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4735
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4548
Fragments arranged by size:
4548 683 56
BtsI GCAGTG_nn'
Cuts at: 2056 2419 2803 3597 3617 2056
Size: 363 384 794 20 3726
Fragments arranged by size:
3726 794 384 363 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5287
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5252
DraI TTT'AAA
Cuts at: 2591 2591
Size: 5287
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4294 809
Size: 507 2978 1802
Fragments arranged by size:
2978 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2734 3623 5252
Size: 54 1425 50 275 18 904 889 1629
Cuts at: 5252 8
Size: 43
Fragments arranged by size:
1629 1425 904 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2734 1537
Size: 275 922 4090
Fragments arranged by size:
4090 922 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3217 1353
Size: 1864 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3874 4248 4394 387
Size: 1064 138 2285 374 146 1280
Fragments arranged by size:
2285 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2251 2426 3350 3947 4734 5002 1239
Size: 393 619 175 924 597 787 268 1524
Fragments arranged by size:
1524 924 787 619 597 393 268 175
EcoRI G'AATT_C
Cuts at: 2596 2974 2596
Size: 378 4909
FspI TGC'GCA
Cuts at: 3793 3793
Size: 5287
HaeII r_GCGC'y
Cuts at: 1678 2193 4434 1678
Size: 515 2241 2531
Fragments arranged by size:
2531 2241 515
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3928 3960 4002 4034 1373
Size: 32 135 32 2356 32 42 32 2626
Fragments arranged by size:
2626 2356 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2631 2877 669
Size: 988 281 693 246 3079
Fragments arranged by size:
3079 988 693 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5287
HpaI GTT'AAC
Cuts at: 2877 2877
Size: 5287
KasI G'GCGC_C
Cuts at: 2189 2189
Size: 5287
KpnI G_GTAC'C
Cuts at: 2612 2612
Size: 5287
MfeI C'AATT_G
Cuts at: 2886 2886
Size: 5287
MluI A'CGCG_T
Cuts at: 5163 5163
Size: 5287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2583 4401 4585 1305
Size: 1278 1818 184 2007
Fragments arranged by size:
2007 1818 1278 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3465
Fragments arranged by size:
3465 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3245 3604 3763 518
Size: 648 2079 359 159 2042
Fragments arranged by size:
2079 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2703 3380 4528 4773 1606
Size: 1097 677 1148 245 2120
Fragments arranged by size:
2120 1148 1097 677 245
NarI GG'CG_CC
Cuts at: 2190 2190
Size: 5287
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4735
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5287
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5287
NotI GC'GGCC_GC
Cuts at: 2734 2734
Size: 5287
NspI r_CATG'y
Cuts at: 1132 1229 2645 4190 1132
Size: 97 1416 1545 2229
Fragments arranged by size:
2229 1545 1416 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5287
PciI A'CATG_T
Cuts at: 4186 4186
Size: 5287
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5287
PfoI T'CCnGG_A
Cuts at: 2334 2334
Size: 5287
PmeI GTTT'AAAC
Cuts at: 2591 2591
Size: 5287
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3356 3388 4869 4901 3356
Size: 32 1481 32 3742
Fragments arranged by size:
3742 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5287
PsiI TTA'TAA
Cuts at: 2857 2857
Size: 5287
PstI C_TGCA'G
Cuts at: 830 2639 830
Size: 1809 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5287
SalI G'TCGA_C
Cuts at: 1936 2629 1936
Size: 693 4594
SbfI CC_TGCA'GG
Cuts at: 2639 2639
Size: 5287
ScaI AGT'ACT
Cuts at: 3535 5247 3535
Size: 1712 3575
SfcI C'TryA_G
Cuts at: 826 1045 2635 3770 4451 4642 826
Size: 219 1590 1135 681 191 1471
Fragments arranged by size:
1590 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2191 2191
Size: 5287
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5287
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5287
SmaI CCC'GGG
Cuts at: 1872 2614 1872
Size: 742 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2236 3391 4292 4554 820
Size: 197 926 293 1155 901 262 1553
Fragments arranged by size:
1553 1155 926 901 293 262 197
SnaBI TAC'GTA
Cuts at: 493 3078 493
Size: 2585 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5287
SphI G_CATG'C
Cuts at: 1132 2645 1132
Size: 1513 3774
SspI AAT'ATT
Cuts at: 5 52 2232 3211 5
Size: 47 2180 979 2081
Fragments arranged by size:
2180 2081 979 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5287
StyI C'CwwG_G
Cuts at: 513 1065 2272 2442 2726 513
Size: 552 1207 170 284 3074
Fragments arranged by size:
3074 1207 552 284 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3314 3331 3484 3669 4114 325
Size: 1255 1734 17 153 185 445 1498
Fragments arranged by size:
1734 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3533 5245 96
Size: 274 80 33 51 1443 1556 1712 138
Fragments arranged by size:
1712 1556 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5247 5279 5247
Size: 32 5255
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5287
XbaI T'CTAG_A
Cuts at: 2623 2623
Size: 5287
XhoI C'TCGA_G
Cuts at: 1943 2236 1943
Size: 293 4994
XmaI C'CCGG_G
Cuts at: 1870 2612 1870
Size: 742 4545
XmnI GAAnn'nnTTC
Cuts at: 3416 3416
Size: 5287
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3095 276
Size: 53 83 186 936 1561 2468
Fragments arranged by size:
2468 1561 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI