(Circular) (Six-base) MAPSORT of: pFN21AE3173.seq Check: 356 from: 1 to: 5923 pFN21AE3173 5923 bp With 182 enzymes: SgfI * March 28, 2012 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3018 3733 278 Size: 53 83 186 936 1482 715 2468 Fragments arranged by size: 2468 1482 936 715 186 83 53 AccI GT'mk_AC Cuts at: 1937 3266 1937 Size: 1329 4594 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2501 2831 3986 5370 5638 1632 Size: 869 330 1155 1384 268 1917 Fragments arranged by size: 1917 1384 1155 869 330 268 AfeI AGC'GCT Cuts at: 3191 3191 Size: 5923 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 4822 5799 1203 Size: 3619 977 1327 Fragments arranged by size: 3619 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AlwNI CAG_nnn'CTG Cuts at: 3666 5238 3666 Size: 1572 4351 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 2344 2841 3232 3458 3610 5719 1493 Size: 851 497 391 226 152 2109 1697 Fragments arranged by size: 2109 1697 851 497 391 226 152 AseI AT'TA_AT Cuts at: 160 1032 2721 4477 160 Size: 872 1689 1756 1606 Fragments arranged by size: 1756 1689 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2954 2990 3248 1870 Size: 73 1011 36 258 4545 Fragments arranged by size: 4545 1011 258 73 36 AvrII C'CTAG_G Cuts at: 2023 3047 2023 Size: 1024 4899 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2921 3244 4699 618 Size: 325 217 627 1134 323 1455 1842 Fragments arranged by size: 1842 1455 1134 627 325 323 217 BanII G_rGCy'C Cuts at: 1391 2401 2648 3242 1391 Size: 1010 247 594 4072 Fragments arranged by size: 4072 1010 594 247 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2287 2460 2758 928 Size: 971 388 173 298 4093 Fragments arranged by size: 4093 971 388 298 173 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4114 4148 1903 Size: 34 2177 34 3678 Fragments arranged by size: 3678 2177 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 2329 1548 Size: 781 5142 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4534 136 Size: 107 122 71 631 605 2862 1525 Fragments arranged by size: 2862 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3156 5918 3156 Size: 2762 3161 BlpI GC'TnA_GC Cuts at: 2117 3340 2117 Size: 1223 4700 Bme1580I G_kGCm'C Cuts at: 1790 3984 5140 1790 Size: 2194 1156 2573 Fragments arranged by size: 2573 2194 1156 BmgBI CAC'GTC Cuts at: 1318 2458 1318 Size: 1140 4783 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2527 3654 4612 Size: 416 53 83 399 24 1110 1127 958 Cuts at: 4612 442 Size: 1753 Fragments arranged by size: 1753 1127 1110 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4583 1239 Size: 3344 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4048 4913 5211 4048 Size: 865 298 4760 Fragments arranged by size: 4760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4586 882 Size: 617 3087 2219 Fragments arranged by size: 3087 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3714 493 Size: 711 2510 2702 Fragments arranged by size: 2702 2510 711 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3015 3730 4112 4747 Size: 53 83 186 936 1482 715 382 635 Cuts at: 4747 275 Size: 1451 Fragments arranged by size: 1482 1451 936 715 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4356 4754 5028 5175 5586 4356 Size: 398 274 147 411 4693 Fragments arranged by size: 4693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5698 Fragments arranged by size: 5698 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2900 2915 2918 1186 Size: 1714 15 3 4191 Fragments arranged by size: 4191 1714 15 3 BseYI C'CCAG_C Cuts at: 2805 5126 5622 2805 Size: 2321 496 3106 Fragments arranged by size: 3106 2321 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5350 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4134 5162 1540 Size: 275 186 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2401 3984 4069 5140 2401 Size: 1583 85 1071 3184 Fragments arranged by size: 3184 1583 1071 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2401 2648 3242 3984 4069 5140 1391 Size: 399 611 247 594 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 611 594 399 247 85 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 2845 3812 5542 2845 Size: 967 1730 3226 Fragments arranged by size: 3226 1730 967 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2313 3278 844 Size: 1469 965 3489 Fragments arranged by size: 3489 1469 965 BsrBI CCG'CTC Cuts at: 1394 1823 3810 1394 Size: 429 1987 3507 Fragments arranged by size: 3507 1987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4418 4592 65 Size: 1183 3170 174 1396 Fragments arranged by size: 3170 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2550 4567 4754 1700 Size: 110 740 2017 187 2869 Fragments arranged by size: 2869 2017 740 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5923 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstYI r'GATC_y Cuts at: 1972 3156 3253 3620 4005 4022 5474 5560 5572 Size: 1184 97 367 385 17 1452 86 12 Cuts at: 5572 5918 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1184 385 367 346 97 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4432 Fragments arranged by size: 4432 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2931 487 Size: 683 56 1705 3479 Fragments arranged by size: 3479 1705 683 56 BtsI GCAGTG_nn' Cuts at: 2398 3439 4233 4253 2398 Size: 1041 794 20 4068 Fragments arranged by size: 4068 1041 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DraIII CAC_nnn'GTG Cuts at: 2932 2932 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2460 3208 3370 Size: 54 1425 50 275 18 630 748 162 Cuts at: 3370 4259 5888 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 748 630 275 162 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3853 1353 Size: 2500 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4510 4884 5030 387 Size: 1064 138 2921 374 146 1280 Fragments arranged by size: 2921 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2399 2399 Size: 5923 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2501 2831 3986 4583 5370 5638 1239 Size: 393 869 330 1155 597 787 268 1524 Fragments arranged by size: 1524 1155 869 787 597 393 330 268 EcoO109I rG'GnC_Cy Cuts at: 2028 2028 Size: 5923 EcoRI G'AATT_C Cuts at: 2344 2841 3232 3610 2344 Size: 497 391 378 4657 Fragments arranged by size: 4657 497 391 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2086 2782 2086 Size: 696 5227 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 3147 3193 5070 1678 Size: 1469 46 1877 2531 Fragments arranged by size: 2531 1877 1469 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2418 2450 4564 4596 4638 Size: 32 135 32 846 32 2114 32 42 Cuts at: 4638 4670 1373 Size: 32 2626 Fragments arranged by size: 2626 2114 846 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5037 5221 1305 Size: 3732 184 2007 Fragments arranged by size: 3732 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3210 10 Size: 54 1425 343 1378 2723 Fragments arranged by size: 2723 1425 1378 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3096 3881 4240 4399 518 Size: 648 1930 785 359 159 2042 Fragments arranged by size: 2042 1930 785 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2113 2809 3339 4016 5164 5409 1606 Size: 507 696 530 677 1148 245 2120 Fragments arranged by size: 2120 1148 696 677 530 507 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4432 Fragments arranged by size: 4432 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 2257 2548 3281 4826 1132 Size: 97 1028 291 733 1545 2229 Fragments arranged by size: 2229 1545 1028 733 291 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5923 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2539 2932 1108 Size: 1431 393 4099 Fragments arranged by size: 4099 1431 393 PfoI T'CCnGG_A Cuts at: 2769 2769 Size: 5923 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2028 2028 Size: 5923 PshAI GACnn'nnGTC Cuts at: 1613 2826 1613 Size: 1213 4710 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PstI C_TGCA'G Cuts at: 830 3275 830 Size: 2445 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 2809 2809 Size: 5923 SacI G_AGCT'C Cuts at: 2401 2401 Size: 5923 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SfcI C'TryA_G Cuts at: 826 1045 2649 2824 3271 4406 5087 5278 826 Size: 219 1604 175 447 1135 681 191 1471 Fragments arranged by size: 1604 1471 1135 681 447 219 191 175 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 2956 2992 3250 1872 Size: 1084 36 258 4545 Fragments arranged by size: 4545 1084 258 36 SmlI C'TyrA_G Cuts at: 820 1017 1943 4027 4928 5190 820 Size: 197 926 2084 901 262 1553 Fragments arranged by size: 2084 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 2004 2023 3047 3362 513 Size: 552 939 19 1024 315 3074 Fragments arranged by size: 3074 1024 939 552 315 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2939 3950 3967 4120 4305 4750 325 Size: 1255 1359 1011 17 153 185 445 1498 Fragments arranged by size: 1498 1359 1255 1011 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2496 3060 4169 Size: 274 80 33 51 1443 519 564 1109 Cuts at: 4169 5881 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1109 564 519 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2512 2544 2864 2896 5883 5915 2512 Size: 32 320 32 2987 32 2520 Fragments arranged by size: 2987 2520 320 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2390 2455 2390 Size: 65 5858 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 2954 2990 3248 1870 Size: 1084 36 258 4545 Fragments arranged by size: 4545 1084 258 36 XmnI GAAnn'nnTTC Cuts at: 2069 2765 4052 2069 Size: 696 1287 3940 Fragments arranged by size: 3940 1287 696 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3016 3731 276 Size: 53 83 186 936 1482 715 2468 Fragments arranged by size: 2468 1482 936 715 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI