(Circular) (Six-base) MAPSORT of: pFN21AE2230.seq Check: 7290 from: 1 to: 6112 pFN21AE2230 6112 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3922 278 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2454 3455 1937 Size: 517 1001 4594 Fragments arranged by size: 4594 1001 517 Acc65I G'GTAC_C Cuts at: 3433 3433 Size: 6112 AclI AA'CG_TT Cuts at: 1563 4239 4612 1563 Size: 2676 373 3063 Fragments arranged by size: 3063 2676 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2213 2542 3110 3170 3176 4175 5559 5827 Size: 581 329 568 60 6 999 1384 268 Cuts at: 5827 1632 Size: 1917 Fragments arranged by size: 1917 1384 999 581 568 329 268 60 6 AflII C'TTAA_G Cuts at: 820 1017 2054 820 Size: 197 1037 4878 Fragments arranged by size: 4878 1037 197 AflIII A'CryG_T Cuts at: 1203 2200 3342 5011 5988 1203 Size: 997 1142 1669 977 1327 Fragments arranged by size: 1669 1327 1142 997 977 AgeI A'CCGG_T Cuts at: 4943 4943 Size: 6112 AhdI GACnn_n'nnGTC Cuts at: 4841 4841 Size: 6112 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6112 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2238 2270 2238 Size: 32 6080 AlwNI CAG_nnn'CTG Cuts at: 2738 3088 3855 5427 2738 Size: 350 767 1572 3423 Fragments arranged by size: 3423 1572 767 350 ApaI G_GGCC'C Cuts at: 3166 3166 Size: 6112 ApaLI G'TGCA_C Cuts at: 3121 4169 5325 3121 Size: 1048 1156 3908 Fragments arranged by size: 3908 1156 1048 ApoI r'AATT_y Cuts at: 1493 2495 2662 2857 2961 3173 3389 3421 3647 Size: 1002 167 195 104 212 216 32 226 Cuts at: 3647 3799 5908 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1002 226 216 212 195 167 152 104 32 AseI AT'TA_AT Cuts at: 160 1032 4666 160 Size: 872 3634 1606 Fragments arranged by size: 3634 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 AvaI C'yCGr_G Cuts at: 1870 1943 3437 1870 Size: 73 1494 4545 Fragments arranged by size: 4545 1494 73 BamHI G'GATC_C Cuts at: 3442 3809 3442 Size: 367 5745 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3433 4888 618 Size: 325 217 627 1646 1455 1842 Fragments arranged by size: 1842 1646 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2359 2389 3166 3243 3431 1391 Size: 968 30 777 77 188 4072 Fragments arranged by size: 4072 968 777 188 77 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3345 928 Size: 971 1446 3695 Fragments arranged by size: 3695 1446 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4303 4337 1903 Size: 34 2366 34 3678 Fragments arranged by size: 3678 2366 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4004 5214 4004 Size: 1210 4902 BclI T'GATC_A Cuts at: 1548 2776 1548 Size: 1228 4884 BfrBI ATG'CAT Cuts at: 2773 2773 Size: 6112 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4723 136 Size: 107 122 71 631 605 3051 1525 Fragments arranged by size: 3051 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6107 6107 Size: 6112 BlpI GC'TnA_GC Cuts at: 3529 3529 Size: 6112 Bme1580I G_kGCm'C Cuts at: 1790 3125 3166 4173 5329 1790 Size: 1335 41 1007 1156 2573 Fragments arranged by size: 2573 1335 1156 1007 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6112 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2353 3843 4801 Size: 416 53 83 399 24 936 1490 958 Cuts at: 4801 442 Size: 1753 Fragments arranged by size: 1753 1490 958 936 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6112 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3257 3257 Size: 6112 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2457 2727 2760 4772 1239 Size: 1218 270 33 2012 2579 Fragments arranged by size: 2579 2012 1218 270 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4237 5102 5400 4237 Size: 865 298 4949 Fragments arranged by size: 4949 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2026 2992 4775 882 Size: 617 527 966 1783 2219 Fragments arranged by size: 2219 1783 966 617 527 BsaAI yAC'GTr Cuts at: 493 1204 3120 3903 493 Size: 711 1916 783 2702 Fragments arranged by size: 2702 1916 783 711 BsaBI GATnn'nnATC Cuts at: 6106 6106 Size: 6112 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3919 4301 4936 275 Size: 53 83 186 936 2386 382 635 1451 Fragments arranged by size: 2386 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3298 4545 4943 5217 5364 5775 3298 Size: 1247 398 274 147 411 3635 Fragments arranged by size: 3635 1247 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2376 2406 1178 Size: 30 165 30 973 30 4884 Fragments arranged by size: 4884 973 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2397 3231 1186 Size: 1211 834 4067 Fragments arranged by size: 4067 1211 834 BseYI C'CCAG_C Cuts at: 3236 5315 5811 3236 Size: 2079 496 3537 Fragments arranged by size: 3537 2079 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2751 1163 Size: 573 1015 4524 Fragments arranged by size: 4524 1015 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3562 4323 5351 1540 Size: 275 186 1561 761 1028 2301 Fragments arranged by size: 2301 1561 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3125 4173 4258 5329 3125 Size: 1048 85 1071 3908 Fragments arranged by size: 3908 1071 1048 85 BsmI GAATG_Cn' Cuts at: 2842 3623 3716 2842 Size: 781 93 5238 Fragments arranged by size: 5238 781 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2359 2389 3125 3166 3243 3431 4173 Size: 399 569 30 736 41 77 188 742 Cuts at: 4173 4258 5329 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 736 569 399 188 85 77 41 30 BspEI T'CCGG_A Cuts at: 5775 5775 Size: 6112 BspHI T'CATG_A Cuts at: 4001 5731 4001 Size: 1730 4382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3467 844 Size: 2623 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3999 1394 Size: 429 2176 3507 Fragments arranged by size: 3507 2176 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2374 2757 4607 4781 65 Size: 1183 1126 383 1850 174 1396 Fragments arranged by size: 1850 1396 1183 1126 383 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4756 4943 1700 Size: 110 2946 187 2869 Fragments arranged by size: 2946 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6112 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6112 BssSI C'ACGA_G Cuts at: 4172 5184 4172 Size: 1012 5100 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6112 BstBI TT'CG_AA Cuts at: 4916 4916 Size: 6112 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6112 BstYI r'GATC_y Cuts at: 1972 3442 3809 4194 4211 5663 5749 5761 6107 Size: 1470 367 385 17 1452 86 12 346 Cuts at: 6107 1972 Size: 1977 Fragments arranged by size: 1977 1470 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2455 2455 Size: 6112 Bsu36I CC'TnA_GG Cuts at: 2037 2037 Size: 6112 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5560 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5373 Fragments arranged by size: 5373 683 56 BtsI GCAGTG_nn' Cuts at: 3069 3628 4422 4442 3069 Size: 559 794 20 4739 Fragments arranged by size: 4739 794 559 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6112 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6077 DraI TTT'AAA Cuts at: 3416 3416 Size: 6112 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5119 809 Size: 507 3803 1802 Fragments arranged by size: 3803 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3135 3559 4448 Size: 54 1425 50 275 18 1305 424 889 Cuts at: 4448 6077 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1305 889 424 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3559 1537 Size: 275 1747 4090 Fragments arranged by size: 4090 1747 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3071 3328 4042 1353 Size: 1718 257 714 3423 Fragments arranged by size: 3423 1718 714 257 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4699 5073 5219 387 Size: 1064 138 3110 374 146 1280 Fragments arranged by size: 3110 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2457 2542 2727 2760 3110 3170 Size: 393 581 244 85 185 33 350 60 Cuts at: 3170 3176 4175 4772 5559 5827 1239 Size: 6 999 597 787 268 1524 Fragments arranged by size: 1524 999 787 597 581 393 350 268 244 185 85 60 33 6 EcoO109I rG'GnC_Cy Cuts at: 3163 3163 Size: 6112 EcoRI G'AATT_C Cuts at: 2857 2961 3421 3799 2857 Size: 104 460 378 5170 Fragments arranged by size: 5170 460 378 104 EcoRV GAT'ATC Cuts at: 2025 2025 Size: 6112 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2080 2534 2080 Size: 454 5658 FspI TGC'GCA Cuts at: 4618 4618 Size: 6112 HaeII r_GCGC'y Cuts at: 1678 5259 1678 Size: 3581 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2376 2408 3299 3331 4753 Size: 32 135 32 804 32 891 32 1422 Cuts at: 4753 4785 4827 4859 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1422 891 804 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3456 3702 669 Size: 988 281 1518 246 3079 Fragments arranged by size: 3079 1518 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6112 HpaI GTT'AAC Cuts at: 3702 3702 Size: 6112 KpnI G_GTAC'C Cuts at: 3437 3437 Size: 6112 MfeI C'AATT_G Cuts at: 3711 3711 Size: 6112 MluI A'CGCG_T Cuts at: 5988 5988 Size: 6112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2332 5226 5410 1305 Size: 1027 2894 184 2007 Fragments arranged by size: 2894 2007 1027 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4290 Fragments arranged by size: 4290 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2394 2594 3308 4070 4429 4588 518 Size: 648 1228 200 714 762 359 159 2042 Fragments arranged by size: 2042 1228 762 714 648 359 200 159 MspA1I CmG'CkG Cuts at: 1606 3077 3085 3265 3528 4205 5353 5598 1606 Size: 1471 8 180 263 677 1148 245 2120 Fragments arranged by size: 2120 1471 1148 677 263 245 180 8 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5560 NdeI CA'TA_TG Cuts at: 387 387 Size: 6112 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6112 NotI GC'GGCC_GC Cuts at: 3559 3559 Size: 6112 NsiI A_TGCA'T Cuts at: 2775 2775 Size: 6112 NspI r_CATG'y Cuts at: 1132 1229 2204 3346 3470 5015 1132 Size: 97 975 1142 124 1545 2229 Fragments arranged by size: 2229 1545 1142 975 124 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6112 PciI A'CATG_T Cuts at: 2200 3342 5011 2200 Size: 1142 1669 3301 Fragments arranged by size: 3301 1669 1142 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6112 PmeI GTTT'AAAC Cuts at: 3416 3416 Size: 6112 PmlI CAC'GTG Cuts at: 3120 3120 Size: 6112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4181 4213 5694 5726 4181 Size: 32 1481 32 4567 Fragments arranged by size: 4567 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6112 PsiI TTA'TAA Cuts at: 3682 3682 Size: 6112 PspOMI G'GGCC_C Cuts at: 3162 3162 Size: 6112 PstI C_TGCA'G Cuts at: 830 3076 3270 3464 830 Size: 2246 194 194 3478 Fragments arranged by size: 3478 2246 194 194 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6112 PvuII CAG'CTG Cuts at: 3085 3265 3085 Size: 180 5932 SalI G'TCGA_C Cuts at: 1936 3454 1936 Size: 1518 4594 SbfI CC_TGCA'GG Cuts at: 3464 3464 Size: 6112 ScaI AGT'ACT Cuts at: 4360 6072 4360 Size: 1712 4400 SfcI C'TryA_G Cuts at: 826 1045 2980 3072 3266 3369 3460 4595 5276 Size: 219 1935 92 194 103 91 1135 681 Cuts at: 5276 5467 826 Size: 191 1471 Fragments arranged by size: 1935 1471 1135 681 219 194 191 103 92 91 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6112 SmaI CCC'GGG Cuts at: 1872 3439 1872 Size: 1567 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2054 4216 5117 5379 820 Size: 197 926 111 2162 901 262 1553 Fragments arranged by size: 2162 1553 926 901 262 197 111 SnaBI TAC'GTA Cuts at: 493 3903 493 Size: 3410 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6112 SphI G_CATG'C Cuts at: 1132 3470 1132 Size: 2338 3774 SspI AAT'ATT Cuts at: 5 52 2323 4036 5 Size: 47 2271 1713 2081 Fragments arranged by size: 2271 2081 1713 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6112 StyI C'CwwG_G Cuts at: 513 1065 3551 513 Size: 552 2486 3074 Fragments arranged by size: 3074 2486 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2973 4139 4156 4309 4494 4939 325 Size: 1255 1393 1166 17 153 185 445 1498 Fragments arranged by size: 1498 1393 1255 1166 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2194 2635 2689 Size: 274 80 33 51 1443 217 441 54 Cuts at: 2689 3014 4358 6070 96 Size: 325 1344 1712 138 Fragments arranged by size: 1712 1443 1344 441 325 274 217 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6072 6104 6072 Size: 32 6080 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6112 XbaI T'CTAG_A Cuts at: 2719 3448 2719 Size: 729 5383 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6112 XmaI C'CCGG_G Cuts at: 1870 3437 1870 Size: 1567 4545 XmnI GAAnn'nnTTC Cuts at: 4241 4241 Size: 6112 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3920 276 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI