(Linear) (Six-base) MAPSORT of: KIEE2230.seq Check: 5536 from: 1 to: 1407 KIEE2230.orf With 182 enzymes: SgfI * July 3, 2012 17:16 .. AccI GT'mk_AC Cuts at: 0 449 1407 Size: 449 958 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 537 1105 1165 1171 1407 Size: 208 329 568 60 6 236 Fragments arranged by size: 568 329 236 208 60 6 AflII C'TTAA_G Cuts at: 0 49 1407 Size: 49 1358 AflIII A'CryG_T Cuts at: 0 195 1337 1407 Size: 195 1142 70 Fragments arranged by size: 1142 195 70 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 233 265 1407 Size: 233 32 1142 Fragments arranged by size: 1142 233 32 AlwNI CAG_nnn'CTG Cuts at: 0 733 1083 1407 Size: 733 350 324 Fragments arranged by size: 733 350 324 ApaI G_GGCC'C Cuts at: 0 1161 1407 Size: 1161 246 ApaLI G'TGCA_C Cuts at: 0 1116 1407 Size: 1116 291 ApoI r'AATT_y Cuts at: 0 490 657 852 956 1168 1384 1407 Size: 490 167 195 104 212 216 23 Fragments arranged by size: 490 216 212 195 167 104 23 BanII G_rGCy'C Cuts at: 0 354 384 1161 1238 1407 Size: 354 30 777 77 169 Fragments arranged by size: 777 354 169 77 30 BbsI GAAGACnn'nnnn_ Cuts at: 0 1340 1407 Size: 1340 67 BclI T'GATC_A Cuts at: 0 771 1407 Size: 771 636 BfrBI ATG'CAT Cuts at: 0 768 1407 Size: 768 639 Bme1580I G_kGCm'C Cuts at: 0 1120 1161 1407 Size: 1120 41 246 Fragments arranged by size: 1120 246 41 BmrI ACTGGGnnnn_n' Cuts at: 0 348 1407 Size: 348 1059 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1252 1407 Size: 1252 155 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 452 722 755 1407 Size: 452 270 33 652 Fragments arranged by size: 652 452 270 33 BsaI GGTCTCn'nnnn_ Cuts at: 0 21 987 1407 Size: 21 966 420 Fragments arranged by size: 966 420 21 BsaAI yAC'GTr Cuts at: 0 1115 1407 Size: 1115 292 BsaWI w'CCGG_w Cuts at: 0 1293 1407 Size: 1293 114 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 371 401 1407 Size: 371 30 1006 Fragments arranged by size: 1006 371 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 392 1226 1407 Size: 392 834 181 Fragments arranged by size: 834 392 181 BseYI C'CCAG_C Cuts at: 0 1231 1407 Size: 1231 176 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 746 1407 Size: 746 661 BsiHKAI G_wGCw'C Cuts at: 0 1120 1407 Size: 1120 287 BsmI GAATG_Cn' Cuts at: 0 837 1407 Size: 837 570 Bsp1286I G_dGCh'C Cuts at: 0 354 384 1120 1161 1238 1407 Size: 354 30 736 41 77 169 Fragments arranged by size: 736 354 169 77 41 30 BsrDI GCAATG_nn' Cuts at: 0 369 752 1407 Size: 369 383 655 Fragments arranged by size: 655 383 369 BstZ17I GTA'TAC Cuts at: 0 450 1407 Size: 450 957 Bsu36I CC'TnA_GG Cuts at: 0 32 1407 Size: 32 1375 BtsI GCAGTG_nn' Cuts at: 0 1064 1407 Size: 1064 343 EaeI y'GGCC_r Cuts at: 0 1130 1407 Size: 1130 277 EarI CTCTTCn'nnn_ Cuts at: 0 1066 1323 1407 Size: 1066 257 84 Fragments arranged by size: 1066 257 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 452 537 722 755 1105 1165 1171 Size: 208 244 85 185 33 350 60 6 Cuts at: 1171 1407 Size: 236 Fragments arranged by size: 350 244 236 208 185 85 60 33 6 EcoO109I rG'GnC_Cy Cuts at: 0 1158 1407 Size: 1158 249 EcoRI G'AATT_C Cuts at: 0 852 956 1407 Size: 852 104 451 Fragments arranged by size: 852 451 104 EcoRV GAT'ATC Cuts at: 0 20 1407 Size: 20 1387 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 75 529 1407 Size: 75 454 878 Fragments arranged by size: 878 454 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 371 403 1294 1326 1407 Size: 371 32 891 32 81 Fragments arranged by size: 891 371 81 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 327 1407 Size: 327 1080 MslI CAynn'nnrTG Cuts at: 0 389 589 1303 1407 Size: 389 200 714 104 Fragments arranged by size: 714 389 200 104 MspA1I CmG'CkG Cuts at: 0 1072 1080 1260 1407 Size: 1072 8 180 147 Fragments arranged by size: 1072 180 147 8 NsiI A_TGCA'T Cuts at: 0 770 1407 Size: 770 637 NspI r_CATG'y Cuts at: 0 199 1341 1407 Size: 199 1142 66 Fragments arranged by size: 1142 199 66 PciI A'CATG_T Cuts at: 0 195 1337 1407 Size: 195 1142 70 Fragments arranged by size: 1142 195 70 PmlI CAC'GTG Cuts at: 0 1115 1407 Size: 1115 292 PspOMI G'GGCC_C Cuts at: 0 1157 1407 Size: 1157 250 PstI C_TGCA'G Cuts at: 0 1071 1265 1407 Size: 1071 194 142 Fragments arranged by size: 1071 194 142 PvuII CAG'CTG Cuts at: 0 1080 1260 1407 Size: 1080 180 147 Fragments arranged by size: 1080 180 147 SfcI C'TryA_G Cuts at: 0 975 1067 1261 1364 1407 Size: 975 92 194 103 43 Fragments arranged by size: 975 194 103 92 43 SmlI C'TyrA_G Cuts at: 0 49 1407 Size: 49 1358 SspI AAT'ATT Cuts at: 0 318 1407 Size: 318 1089 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 968 1407 Size: 968 439 TatI w'GTAC_w Cuts at: 0 189 630 684 1009 1407 Size: 189 441 54 325 398 Fragments arranged by size: 441 398 325 189 54 XbaI T'CTAG_A Cuts at: 0 714 1407 Size: 714 693 Enzymes that do cut: AccI AcuI AflII AflIII AloI AlwNI ApaI ApaLI ApoI BanII BbsI BclI BfrBI Bme1580I BmrI BplI BpmI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI BstZ17I Bsu36I BtsI EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I MmeI MslI MspA1I NsiI NspI PciI PmlI PspOMI PstI PvuII SfcI SmlI SspI TaqII TatI XbaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AhdI AleI AlfI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BglI BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI StyI SwaI TstI Tth111I XcmI XhoI XmaI XmnI ZraI