Restriction map for
pFN21AE2518
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# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AgeI ApaI AscI
Asp718I AspI AviII AvrII BalI
BbrPI BbvCI BglI BlnI BsaBI
BsgI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BsrBI BsrGI BssHII ClaI
CpoI DraIII EagI EclXI Eco52I
EcoRV FseI FspI HpaI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI NaeI NgoMIV NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PvuI RsrII SacII
SalI SapI ScaI SexAI SfiI
SgrAI SmaI SnaBI SphI SspBI
Tth111I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccI 468 GTATAC GT^[AC][GT]AC
AccII 2471 CGCG CG^CG
AccIII 1938 TCCGGA T^CCGGA
AclI 1329 AACGTT AA^CGTT
AflII 1668 CTTAAG C^TTAAG
AhdI 3074 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4246 GTGCAC G^TGCAC
AocI 821 CCTCAGG CC^T[ACGT]AGG
Aor51HI 98 AGCGCT AGC^GCT
ApaLI 4246 GTGCAC G^TGCAC
AspEI 3074 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2027 CTCGAG C^[CT]CG[GA]G
BamHI 3402 GGATCC G^GATCC
BciVI 1199 GTATCC GTATCC(6/5)
BclI 2594 TGATCA T^GATCA
BfrI 1668 CTTAAG C^TTAAG
BlpI 754 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 754 GCTGAGC GC^T[ACGT]AGC
BsaAI 2644 TACGTG [CT]AC^GT[GA]
BseAI 1938 TCCGGA T^CCGGA
BsiEI 559 CGGTCG CG[GA][CT]^CG
BsoBI 2027 CTCGAG C^[CT]CG[GA]G
BspEI 1938 TCCGGA T^CCGGA
BsrFI 1108 GCCGGT [GA]^CCGG[CT]
Bst1107I 468 GTATAC GTA^TAC
BstBI 3791 TTCGAA TT^CGAA
BstUI 2471 CGCG CG^CG
BstXI 3392 CCAGCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 468 GTATAC GTA^TAC
Bsu36I 821 CCTCAGG CC^T[ACGT]AGG
CelII 754 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1108 GCCGGT [GA]^CCGG[CT]
DraII 1318 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 839 GACTTAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3074 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1744 GAAGAG CTCTTC(1/4)
Eco47III 98 AGCGCT AGC^GCT
Eco81I 821 CCTCAGG CC^T[ACGT]AGG
EcoNI 2164 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1318 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 3416 ATGCAT ATGCA^T
FauI 669 CCCGC CCCGC(4/6)
FbaI 2594 TGATCA T^GATCA
HgaI 4009 GCGTC GACGC(5/10)
HincII 3952 GTCAAC GT[CT]^[GA]AC
HindII 3952 GTCAAC GT[CT]^[GA]AC
Ksp632I 1744 GAAGAG CTCTTC(1/4)
MroI 1938 TCCGGA T^CCGGA
MvnI 2471 CGCG CG^CG
NheI 2062 GCTAGC G^CTAGC
NsiI 3416 ATGCAT ATGCA^T
NspV 3791 TTCGAA TT^CGAA
PaeR7I 2027 CTCGAG C^TCGAG
PflMI 3878 CCAATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1318 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 880 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1329 AACGTT AA^CGTT
PvuII 926 CAGCTG CAG^CTG
SbfI 1169 CCTGCAGG CCTGCA^GG
SfuI 3791 TTCGAA TT^CGAA
Sse8387I 1169 CCTGCAGG CCTGCA^GG
StuI 1672 AGGCCT AGG^CCT
SwaI 210 ATTTAAAT ATTT^AAAT
Van91I 3878 CCAATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2027 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 1951 GGCGCC G[GA]^CG[CT]C
4264 GGCGCC
AseI 2103 ATTAAT AT^TAAT
2299 ATTAAT
AsnI 2103 ATTAAT AT^TAAT
2299 ATTAAT
BbeI 1951 GGCGCC GGCGC^C
4264 GGCGCC
BcgI 2473 CGAGCGTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1184 GCAGTGACCTCG
BglII 419 AGATCT A^GATCT
4326 AGATCT
BpmI 447 CTCCAG CTGGAG(16/14)
3269 CTCCAG
BsaHI 1951 GGCGCC G[GA]^CG[CT]C
4264 GGCGCC
BsaI 2952 GGTCTC GGTCTC(1/5)
3942 GGTCTC
BsaWI 1938 TCCGGA [AT]^CCGG[AT]
3939 TCCGGT
BseRI 2375 CTCCTC GAGGAG(10/8)
3077 GAGGAG
BssSI 546 CACGAG CACGAG(-5/-1)
4330 CTCGTG
BstAPI 496 GCAATCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2566 GCACAGTCTGC
DraI 211 TTTAAA TTT^AAA
1038 TTTAAA
DsaI 2459 CCATGG C^C[GA][CT]GG
3723 CCATGG
EaeI 1099 TGGCCG [CT]^GGCC[GA]
4163 TGGCCG
EcoRI 1216 GAATTC G^AATTC
1767 GAATTC
Hin1I 1951 GGCGCC G[GA]^CG[CT]C
4264 GGCGCC
KasI 1951 GGCGCC G^GCGCC
4264 GGCGCC
NarI 1951 GGCGCC GG^CGCC
4264 GGCGCC
NcoI 2459 CCATGG C^CATGG
3723 CCATGG
NspI 3565 GCATGT [GA]CATG^[CT]
4005 ACATGC
PshBI 2103 ATTAAT AT^TAAT
2299 ATTAAT
SacI 757 GAGCTC GAGCT^C
2042 GAGCTC
SfoI 1951 GGCGCC GGC^GCC
4264 GGCGCC
SpeI 1654 ACTAGT A^CTAGT
4312 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EcoO65I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
PleI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI