(Circular) (Six-base) MAPSORT of: pFN21AE2518.seq Check: 9791 from: 1 to: 9073 pFN21AE2518 9073 bp With 182 enzymes: SgfI * February 23, 2015 12:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2406 3182 2406 Size: 776 8297 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6883 278 Size: 53 83 186 936 5347 2468 Fragments arranged by size: 5347 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2474 6416 1937 Size: 537 3942 4594 Fragments arranged by size: 4594 3942 537 Acc65I G'GTAC_C Cuts at: 6394 6394 Size: 9073 AclI AA'CG_TT Cuts at: 1563 3335 7200 7573 1563 Size: 1772 3865 373 3063 Fragments arranged by size: 3865 3063 1772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4155 4425 7136 8520 8788 1632 Size: 2523 270 2711 1384 268 1917 Fragments arranged by size: 2711 2523 1917 1384 270 268 AfeI AGC'GCT Cuts at: 2105 2105 Size: 9073 AflII C'TTAA_G Cuts at: 820 1017 3673 820 Size: 197 2656 6220 Fragments arranged by size: 6220 2656 197 AflIII A'CryG_T Cuts at: 1203 4650 5058 5458 6247 7972 8949 1203 Size: 3447 408 400 789 1725 977 1327 Fragments arranged by size: 3447 1725 1327 977 789 408 400 AgeI A'CCGG_T Cuts at: 7904 7904 Size: 9073 AhdI GACnn_n'nnGTC Cuts at: 5084 7802 5084 Size: 2718 6355 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9073 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9073 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3636 3668 4180 4212 5046 5078 3636 Size: 32 512 32 834 32 7631 Fragments arranged by size: 7631 834 512 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3010 4403 5025 6816 8388 3010 Size: 1393 622 1791 1572 3695 Fragments arranged by size: 3695 1791 1572 1393 622 ApaLI G'TGCA_C Cuts at: 6251 7130 8286 6251 Size: 879 1156 7038 Fragments arranged by size: 7038 1156 879 ApoI r'AATT_y Cuts at: 1493 2174 2291 2639 3046 3221 3772 3869 4495 Size: 681 117 348 407 175 551 97 626 Cuts at: 4495 4717 4852 5342 6382 6608 6760 8869 1493 Size: 222 135 490 1040 226 152 2109 1697 Fragments arranged by size: 2109 1697 1040 681 626 551 490 407 348 226 222 175 152 135 117 97 AseI AT'TA_AT Cuts at: 160 1032 4109 4305 7627 160 Size: 872 3077 196 3322 1606 Fragments arranged by size: 3322 3077 1606 872 196 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9073 AvaI C'yCGr_G Cuts at: 1870 1943 4032 6398 1870 Size: 73 2089 2366 4545 Fragments arranged by size: 4545 2366 2089 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4771 4804 4771 Size: 33 9040 BamHI G'GATC_C Cuts at: 5407 6403 6770 5407 Size: 996 367 7710 Fragments arranged by size: 7710 996 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2724 3956 6269 6394 7849 Size: 325 217 627 937 1232 2313 125 1455 Cuts at: 7849 618 Size: 1842 Fragments arranged by size: 2313 1842 1455 1232 937 627 325 217 125 BanII G_rGCy'C Cuts at: 1391 2766 4051 5556 6392 1391 Size: 1375 1285 1505 836 4072 Fragments arranged by size: 4072 1505 1375 1285 836 BbeI G_GCGC'C Cuts at: 3960 6273 3960 Size: 2313 6760 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3639 5609 5786 6009 6150 928 Size: 971 1740 1970 177 223 141 3851 Fragments arranged by size: 3851 1970 1740 971 223 177 141 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3178 3212 4467 4501 7264 7298 1903 Size: 34 1241 34 1255 34 2763 34 3678 Fragments arranged by size: 3678 2763 1255 1241 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3215 6965 8175 3215 Size: 3750 1210 4113 Fragments arranged by size: 4113 3750 1210 BclI T'GATC_A Cuts at: 1548 4599 1548 Size: 3051 6022 BfrBI ATG'CAT Cuts at: 5423 5423 Size: 9073 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7684 136 Size: 107 122 71 631 605 6012 1525 Fragments arranged by size: 6012 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2424 6331 9068 2424 Size: 3907 2737 2429 Fragments arranged by size: 3907 2737 2429 BlpI GC'TnA_GC Cuts at: 2760 6490 2760 Size: 3730 5343 Bme1580I G_kGCm'C Cuts at: 1790 6255 7134 8290 1790 Size: 4465 879 1156 2573 Fragments arranged by size: 4465 2573 1156 879 BmgBI CAC'GTC Cuts at: 1318 5922 6248 1318 Size: 4604 326 4143 Fragments arranged by size: 4604 4143 326 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2121 2260 3020 Size: 416 53 83 399 24 704 139 760 Cuts at: 3020 3430 3895 4228 5040 5110 5548 5779 6804 Size: 410 465 333 812 70 438 231 1025 Cuts at: 6804 7762 442 Size: 958 1753 Fragments arranged by size: 1753 1025 958 812 760 704 465 438 416 410 399 333 231 139 83 70 53 24 BmtI G_CTAG'C Cuts at: 1056 4071 1056 Size: 3015 6058 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3180 3180 Size: 9073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2437 5259 7733 1239 Size: 1198 2822 2474 2579 Fragments arranged by size: 2822 2579 2474 1198 Bpu10I CC'TnA_GC Cuts at: 2768 4365 2768 Size: 1597 7476 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4368 6246 7198 8063 8361 4368 Size: 1878 952 865 298 5080 Fragments arranged by size: 5080 1878 952 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4963 5953 7736 882 Size: 617 3464 990 1783 2219 Fragments arranged by size: 3464 2219 1783 990 617 BsaAI yAC'GTr Cuts at: 493 1204 4651 6864 493 Size: 711 3447 2213 2702 Fragments arranged by size: 3447 2702 2213 711 BsaBI GATnn'nnATC Cuts at: 9067 9067 Size: 9073 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3957 6270 6880 7262 Size: 53 83 186 936 2424 2313 610 382 Cuts at: 7262 7897 275 Size: 635 1451 Fragments arranged by size: 2424 2313 1451 936 635 610 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3943 5944 7506 7904 8178 8325 8736 3943 Size: 2001 1562 398 274 147 411 4280 Fragments arranged by size: 4280 2001 1562 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2004 2034 3910 3940 4180 Size: 30 165 30 601 30 1876 30 240 Cuts at: 4180 4210 4363 4393 5257 5287 1178 Size: 30 153 30 864 30 4964 Fragments arranged by size: 4964 1876 864 601 240 165 153 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4371 5097 1186 Size: 3185 726 5162 Fragments arranged by size: 5162 3185 726 BseYI C'CCAG_C Cuts at: 2558 3240 8276 8772 2558 Size: 682 5036 496 2859 Fragments arranged by size: 5036 2859 682 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 8500 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2567 6523 7284 8312 1540 Size: 275 186 566 3956 761 1028 2301 Fragments arranged by size: 3956 2301 1028 761 566 275 186 BsiHKAI G_wGCw'C Cuts at: 2453 2766 4051 6255 7134 7219 8290 2453 Size: 313 1285 2204 879 85 1071 3236 Fragments arranged by size: 3236 2204 1285 1071 879 313 85 BsmI GAATG_Cn' Cuts at: 2510 3616 5400 6584 6677 2510 Size: 1106 1784 1184 93 4906 Fragments arranged by size: 4906 1784 1184 1106 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2453 2766 4051 5556 6255 6392 7134 Size: 399 663 313 1285 1505 699 137 742 Cuts at: 7134 7219 8290 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1505 1285 1071 742 699 663 399 313 137 85 BspEI T'CCGG_A Cuts at: 3943 8736 3943 Size: 4793 4280 BspHI T'CATG_A Cuts at: 2785 5331 6103 6962 8692 2785 Size: 2546 772 859 1730 3166 Fragments arranged by size: 3166 2546 1730 859 772 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2406 3182 5222 6428 844 Size: 1562 776 2040 1206 3489 Fragments arranged by size: 3489 2040 1562 1206 776 BsrBI CCG'CTC Cuts at: 1394 1823 6960 1394 Size: 429 5137 3507 Fragments arranged by size: 5137 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2494 5611 5623 7568 7742 65 Size: 1183 1246 3117 12 1945 174 1396 Fragments arranged by size: 3117 1945 1396 1246 1183 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 3113 7717 7904 1700 Size: 110 1303 4604 187 2869 Fragments arranged by size: 4604 2869 1303 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9073 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9073 BssSI C'ACGA_G Cuts at: 2551 6335 7133 8145 2551 Size: 3784 798 1012 3479 Fragments arranged by size: 3784 3479 1012 798 BstAPI GCAn_nnn'nTGC Cuts at: 794 2507 4577 794 Size: 1713 2070 5290 Fragments arranged by size: 5290 2070 1713 BstBI TT'CG_AA Cuts at: 5797 7877 5797 Size: 2080 6993 BstEII G'GTnAC_C Cuts at: 1184 2913 4277 5111 1184 Size: 1729 1364 834 5146 Fragments arranged by size: 5146 1729 1364 834 BstXI CCAn_nnnn'nTGG Cuts at: 5404 5404 Size: 9073 BstYI r'GATC_y Cuts at: 1972 2424 5407 5584 5974 6331 6403 6770 7155 Size: 452 2983 177 390 357 72 367 385 Cuts at: 7155 7172 8624 8710 8722 9068 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2983 1977 1452 452 390 385 367 357 346 177 86 72 17 12 BstZ17I GTA'TAC Cuts at: 2475 2475 Size: 9073 Bsu36I CC'TnA_GG Cuts at: 2827 2827 Size: 9073 BtgI C'CryG_G Cuts at: 513 1065 4464 5728 513 Size: 552 3399 1264 3858 Fragments arranged by size: 3858 3399 1264 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8334 Fragments arranged by size: 8334 683 56 BtsI GCAGTG_nn' Cuts at: 2124 2656 2882 3196 6589 7383 7403 2124 Size: 532 226 314 3393 794 20 3794 Fragments arranged by size: 3794 3393 794 532 314 226 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9073 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9038 DraI TTT'AAA Cuts at: 2218 3045 6377 2218 Size: 827 3332 4914 Fragments arranged by size: 4914 3332 827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2850 8080 809 Size: 507 1534 5230 1802 Fragments arranged by size: 5230 1802 1534 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3104 6168 6520 Size: 54 1425 50 275 18 1274 3064 352 Cuts at: 6520 7409 9038 8 Size: 889 1629 43 Fragments arranged by size: 3064 1629 1425 1274 889 352 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6520 1537 Size: 275 4708 4090 Fragments arranged by size: 4708 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3744 7003 1353 Size: 2391 3259 3423 Fragments arranged by size: 3423 3259 2391 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7660 8034 8180 387 Size: 1064 138 6071 374 146 1280 Fragments arranged by size: 6071 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2764 4049 2764 Size: 1285 7788 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2437 4155 4425 5259 7136 7733 8520 Size: 393 805 1718 270 834 1877 597 787 Cuts at: 8520 8788 1239 Size: 268 1524 Fragments arranged by size: 1877 1718 1524 834 805 787 597 393 270 268 EcoNI CCTnn'n_nnAGG Cuts at: 4173 4173 Size: 9073 EcoO109I rG'GnC_Cy Cuts at: 3324 3324 Size: 9073 EcoRI G'AATT_C Cuts at: 3221 3772 6382 6760 3221 Size: 551 2610 378 5534 Fragments arranged by size: 5534 2610 551 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5006 5006 Size: 9073 FspI TGC'GCA Cuts at: 7579 7579 Size: 9073 HaeII r_GCGC'y Cuts at: 1678 2107 3960 6273 8220 1678 Size: 429 1853 2313 1947 2531 Fragments arranged by size: 2531 2313 1947 1853 429 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2150 2182 4559 4591 4637 Size: 32 135 32 578 32 2377 32 46 Cuts at: 4637 4669 5070 5102 5257 5289 5479 5511 6165 Size: 32 401 32 155 32 190 32 654 Cuts at: 6165 6197 7714 7746 7788 7820 1373 Size: 32 1517 32 42 32 2626 Fragments arranged by size: 2626 2377 1517 654 578 401 190 155 135 46 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5959 6417 6663 669 Size: 988 281 4021 458 246 3079 Fragments arranged by size: 4021 3079 988 458 281 246 HindIII A'AGCT_T Cuts at: 748 2656 3973 4929 5375 5778 748 Size: 1908 1317 956 446 403 4043 Fragments arranged by size: 4043 1908 1317 956 446 403 HpaI GTT'AAC Cuts at: 6663 6663 Size: 9073 KasI G'GCGC_C Cuts at: 3956 6269 3956 Size: 2313 6760 KpnI G_GTAC'C Cuts at: 6398 6398 Size: 9073 MfeI C'AATT_G Cuts at: 6672 6672 Size: 9073 MluI A'CGCG_T Cuts at: 8949 8949 Size: 9073 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2547 4829 5277 6186 8187 8371 1305 Size: 1242 2282 448 909 2001 184 2007 Fragments arranged by size: 2282 2007 2001 1242 909 448 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7251 Fragments arranged by size: 7251 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3138 4805 4834 5428 5616 6185 7031 Size: 648 1972 1667 29 594 188 569 846 Cuts at: 7031 7390 7549 518 Size: 359 159 2042 Fragments arranged by size: 2042 1972 1667 846 648 594 569 359 188 159 29 MspA1I CmG'CkG Cuts at: 1606 2677 2868 2933 6489 7166 8314 8559 1606 Size: 1071 191 65 3556 677 1148 245 2120 Fragments arranged by size: 3556 2120 1148 1071 677 245 191 65 NarI GG'CG_CC Cuts at: 3957 6270 3957 Size: 2313 6760 NcoI C'CATG_G Cuts at: 513 1065 4464 5728 513 Size: 552 3399 1264 3858 Fragments arranged by size: 3858 3399 1264 552 NdeI CA'TA_TG Cuts at: 387 4159 4210 5237 387 Size: 3772 51 1027 4223 Fragments arranged by size: 4223 3772 1027 51 NheI G'CTAG_C Cuts at: 1052 4067 1052 Size: 3015 6058 NotI GC'GGCC_GC Cuts at: 6520 6520 Size: 9073 NsiI A_TGCA'T Cuts at: 5425 5425 Size: 9073 NspI r_CATG'y Cuts at: 1132 1229 5574 6014 6431 7976 1132 Size: 97 4345 440 417 1545 2229 Fragments arranged by size: 4345 2229 1545 440 417 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9073 PciI A'CATG_T Cuts at: 7972 7972 Size: 9073 PflMI CCAn_nnn'nTGG Cuts at: 1108 5889 1108 Size: 4781 4292 PfoI T'CCnGG_A Cuts at: 2965 2965 Size: 9073 PmeI GTTT'AAAC Cuts at: 6377 6377 Size: 9073 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3743 3775 4180 4212 7142 7174 8655 8687 3743 Size: 32 405 32 2930 32 1481 32 4129 Fragments arranged by size: 4129 2930 1481 405 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3324 3324 Size: 9073 PshAI GACnn'nnGTC Cuts at: 1613 2889 1613 Size: 1276 7797 PsiI TTA'TAA Cuts at: 6237 6643 6237 Size: 406 8667 PstI C_TGCA'G Cuts at: 830 3179 3518 5439 5836 6425 830 Size: 2349 339 1921 397 589 3478 Fragments arranged by size: 3478 2349 1921 589 397 339 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9073 PvuII CAG'CTG Cuts at: 2933 2933 Size: 9073 SacI G_AGCT'C Cuts at: 2766 4051 2766 Size: 1285 7788 SalI G'TCGA_C Cuts at: 1936 6415 1936 Size: 4479 4594 SanDI GG'GwC_CC Cuts at: 3324 3324 Size: 9073 SbfI CC_TGCA'GG Cuts at: 3179 6425 3179 Size: 3246 5827 ScaI AGT'ACT Cuts at: 7321 9033 7321 Size: 1712 7361 SfcI C'TryA_G Cuts at: 826 1045 3175 3514 4858 5413 5435 5832 6421 Size: 219 2130 339 1344 555 22 397 589 Cuts at: 6421 7556 8237 8428 826 Size: 1135 681 191 1471 Fragments arranged by size: 2130 1471 1344 1135 681 589 555 397 339 219 191 22 SfoI GGC'GCC Cuts at: 3958 6271 3958 Size: 2313 6760 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9073 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9073 SmaI CCC'GGG Cuts at: 1872 6400 1872 Size: 4528 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3673 4032 4383 6261 7177 8078 Size: 197 926 1730 359 351 1878 916 901 Cuts at: 8078 8340 820 Size: 262 1553 Fragments arranged by size: 1878 1730 1553 926 916 901 359 351 262 197 SnaBI TAC'GTA Cuts at: 493 6864 493 Size: 6371 2702 SpeI A'CTAG_T Cuts at: 152 3659 6317 152 Size: 3507 2658 2908 Fragments arranged by size: 3507 2908 2658 SphI G_CATG'C Cuts at: 1132 6431 1132 Size: 5299 3774 SspI AAT'ATT Cuts at: 5 52 2383 3689 6037 6346 6997 5 Size: 47 2331 1306 2348 309 651 2081 Fragments arranged by size: 2348 2331 2081 1306 651 309 47 StuI AGG'CCT Cuts at: 1517 3679 1517 Size: 2162 6911 StyI C'CwwG_G Cuts at: 513 1065 3481 4464 5728 6512 513 Size: 552 2416 983 1264 784 3074 Fragments arranged by size: 3074 2416 1264 983 784 552 SwaI ATTT'AAAT Cuts at: 2218 2218 Size: 9073 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2173 3779 3921 4043 4401 4813 6355 Size: 1255 593 1606 142 122 358 412 1542 Cuts at: 6355 7100 7117 7270 7455 7900 325 Size: 745 17 153 185 445 1498 Fragments arranged by size: 1606 1542 1498 1255 745 593 445 412 358 185 153 142 122 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3303 7319 9031 Size: 274 80 33 51 1443 1326 4016 1712 Cuts at: 9031 96 Size: 138 Fragments arranged by size: 4016 1712 1443 1326 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2160 2192 3910 3942 4486 4518 4626 4658 5959 Size: 32 1718 32 544 32 108 32 1301 Cuts at: 5959 5983 5991 6015 6032 6064 9033 9065 2160 Size: 24 8 24 17 32 2969 32 2168 Fragments arranged by size: 2969 2168 1718 1301 544 108 32 32 32 32 32 32 24 24 17 8 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9073 XbaI T'CTAG_A Cuts at: 6409 6409 Size: 9073 XhoI C'TCGA_G Cuts at: 1943 4032 1943 Size: 2089 6984 XmaI C'CCGG_G Cuts at: 1870 6398 1870 Size: 4528 4545 XmnI GAAnn'nnTTC Cuts at: 3000 3753 4168 5793 5803 7202 3000 Size: 753 415 1625 10 1399 4871 Fragments arranged by size: 4871 1625 1399 753 415 10 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6881 276 Size: 53 83 186 936 5347 2468 Fragments arranged by size: 5347 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BbvCI BsiWI BsmBI DraIII EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI XcmI