################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanII BbrPI BcgI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FauI FseI FspI HgaI HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 1435 GTATAC GT^[AC][GT]AC AccII 1257 CGCG CG^CG AcyI 1428 GGCGCC G[GA]^CG[CT]C AflII 403 CTTAAG C^TTAAG AlwNI 934 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 127 CCTAAGG CC^T[ACGT]AGG Asp700I 1055 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 486 GGATCC G^GATCC BbeI 1428 GGCGCC GGCGC^C BciVI 591 GTATCC GTATCC(6/5) BclI 963 TGATCA T^GATCA BfrI 403 CTTAAG C^TTAAG BglII 798 AGATCT A^GATCT BsaHI 1428 GGCGCC G[GA]^CG[CT]C BsmI 1497 GAATGC GAATGC(1/-1) Bst1107I 1435 GTATAC GTA^TAC BstUI 1257 CGCG CG^CG BstZ17I 1435 GTATAC GTA^TAC Bsu36I 127 CCTAAGG CC^T[ACGT]AGG DraI 691 TTTAAA TTT^AAA Eco81I 127 CCTAAGG CC^T[ACGT]AGG EcoRV 91 GATATC GAT^ATC EcoT14I 482 CCAAGG C^C[AT][AT]GG FbaI 963 TGATCA T^GATCA HaeII 1428 GGCGCC [GA]GCGC^[CT] Hin1I 1428 GGCGCC G[GA]^CG[CT]C HincII 570 GTCAAC GT[CT]^[GA]AC HindII 570 GTCAAC GT[CT]^[GA]AC KasI 1428 GGCGCC G^GCGCC MslI 622 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1300 CAGCTG C[AC]G^C[GT]G MvnI 1257 CGCG CG^CG NarI 1428 GGCGCC GG^CGCC NspI 211 GCATGT [GA]CATG^[CT] PvuII 1300 CAGCTG CAG^CTG SexAI 1241 ACCTGGT A^CC[AT]GGT SfaNI 936 GATGC GCATC(5/9) SfoI 1428 GGCGCC GGC^GCC StyI 482 CCAAGG C^C[AT][AT]GG TaqI 26 TCGA T^CGA TfiI 1164 GAATC G^A[AT]TC TthHB8I 26 TCGA T^CGA XmnI 1055 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 233 AAATTT [GA]^AATT[CT] 352 GAATTT ApoI 233 AAATTT [GA]^AATT[CT] 352 GAATTT BcnI 1293 CCCGG CC^[GC]GG 1561 CCCGG BpmI 1273 CTCCAG CTGGAG(16/14) 698 CTGGAG BseRI 1223 CTCCTC GAGGAG(10/8) 1445 CTCCTC BsmFI 1017 GGGAC GGGAC(10/14) 1327 GTCCC BsrDI 388 GCAATG GCAATG(2/0) 1000 GCAATG Cac8I 189 GCAGGC GC[ACGT]^[ACGT]GC 463 GCAAGC CfoI 1258 GCGC GCG^C 1429 GCGC EarI 200 CTCTTC CTCTTC(1/4) 155 GAAGAG HhaI 1258 GCGC GCG^C 1429 GCGC HinP1I 1258 GCGC G^CGC 1429 GCGC HphI 748 GGTGA GGTGA(8/7) 1008 TCACC Ksp632I 200 CTCTTC CTCTTC(1/4) 155 GAAGAG NciI 1293 CCCGG CC^[GC]GG 1561 CCCGG PflMI 427 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1317 CCAGGCAGTGG PleI 198 GACTC GAGTC(4/5) 1365 GACTC PpuMI 1017 GGGACCT [GA]G^G[AT]CC[CT] 1503 AGGACCT PstI 446 CTGCAG CTGCA^G 794 CTGCAG SmlI 403 CTTAAG C^T[CT][GA]AG 550 CTCAAG Van91I 427 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1317 CCAGGCAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BanI BbsI BbvCI BbvI BfaI BpuAI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI Tru9I TseI Tsp45I Tsp509I TspRI XhoII