Restriction map for
pFN21AE1928
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BseAI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BsrI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII CpoI DraI DraIII
DrdI DsaI Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI StuI SwaI Van91I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AhdI 631 GACAATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 387 GATCC GGATC(4/5)
AocI 1266 CCTGAGG CC^T[ACGT]AGG
AspEI 631 GACAATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 93 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 55 GGCACC G^G[CT][GA]CC
BcgI 35 GCAGGATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1346 CTAG C^TAG
BsaAI 88 TACGTG [CT]AC^GT[GA]
BsgI 967 GTGCAG GTGCAG(16/14)
BsiEI 67 CGGCCG CG[GA][CT]^CG
BsiWI 813 CGTACG C^GTACG
BsmBI 638 CGTCTC CGTCTC(1/5)
BspDI 766 ATCGAT AT^CGAT
BssHII 125 GCGCGC G^CGCGC
BstYI 386 AGATCC [GA]^GATC[CT]
Bsu36I 1266 CCTGAGG CC^T[ACGT]AGG
ClaI 766 ATCGAT AT^CGAT
DpnII 387 GATC ^GATC
DraII 440 GGGACCT [GA]G^G[ACGT]CC[CT]
EagI 67 CGGCCG C^GGCCG
Eam1105I 631 GACAATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 67 CGGCCG C^GGCCG
Eco52I 67 CGGCCG C^GGCCG
Eco81I 1266 CCTGAGG CC^T[ACGT]AGG
EcoO109I 440 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 420 CCAAGG C^C[AT][AT]GG
HincII 628 GTTGAC GT[CT]^[GA]AC
HindII 628 GTTGAC GT[CT]^[GA]AC
MaeI 1346 CTAG C^TAG
MboI 387 GATC ^GATC
MflI 386 AGATCC [GA]^GATC[CT]
NdeII 387 GATC ^GATC
NspI 756 GCATGT [GA]CATG^[CT]
PaeR7I 403 CTCGAG C^TCGAG
PflFI 93 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 440 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 811 GACGTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI 387 GATC ^GATC
SexAI 7 ACCAGGT A^CC[AT]GGT
StyI 420 CCAAGG C^C[AT][AT]GG
Tsp509I 563 AATT ^AATT
TspRI 948 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 93 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 403 CTCGAG C^TCGAG
XhoII 386 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 268 GACGCC G[GA]^CG[CT]C
919 GACGCC
AlwNI 322 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
727 CAGGAGCTG
AspHI 72 GAGCTC G[AT]GC[AT]^C
400 GAGCTC
BanII 72 GAGCTC G[GA]GC[CT]^C
400 GAGCTC
BpmI 75 CTCCAG CTGGAG(16/14)
370 CTGGAG
BsaHI 268 GACGCC G[GA]^CG[CT]C
919 GACGCC
BsaWI 908 ACCGGA [AT]^CCGG[AT]
956 ACCGGA
BsiHKAI 72 GAGCTC G[AT]GC[AT]^C
400 GAGCTC
BsmAI 960 GAGAC GTCTC(1/5)
639 GTCTC
BsrFI 65 GCCGGC [GA]^CCGG[CT]
473 GCCGGC
Cfr10I 65 GCCGGC [GA]^CCGG[CT]
473 GCCGGC
Hin1I 268 GACGCC G[GA]^CG[CT]C
919 GACGCC
HphI 977 TCACC GGTGA(8/7)
1134 TCACC
MslI 1135 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1381 CATCACGATG
NaeI 65 GCCGGC GCC^GGC
473 GCCGGC
NgoMIV 65 GCCGGC G^CCGGC
473 GCCGGC
NlaIII 546 CATG CATG^
757 CATG
SacI 72 GAGCTC GAGCT^C
400 GAGCTC
SmlI 403 CTCGAG C^T[CT][GA]AG
412 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
EaeI EarI EcoNI EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeII MaeIII MboII MnlI
MseI MspI MvaI MvnI MwoI
NciI NlaIV PleI PstI RsaI
Sau96I SbfI ScrFI SfaNI SfcI
SmaI Sse8387I TaqI TfiI Tru9I
TseI Tsp45I TthHB8I XcmI XmaCI
XmaI