Restriction map for
pFN21AE1928
(Circular) (Six-base) MAPSORT of: pFN21AE1928.seq Check: 9162 from: 1 to: 6103
pFN21AE1928 6103 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2473 2473
Size: 6103
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3913 278
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3446 1937
Size: 1509 4594
Acc65I G'GTAC_C
Cuts at: 3424 3424
Size: 6103
AclI AA'CG_TT
Cuts at: 1563 4230 4603 1563
Size: 2667 373 3063
Fragments arranged by size:
3063 2667 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2570 4166 5550 5818 1632
Size: 938 1596 1384 268 1917
Fragments arranged by size:
1917 1596 1384 938 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5906
AflIII A'CryG_T
Cuts at: 1203 5002 5979 1203
Size: 3799 977 1327
Fragments arranged by size:
3799 1327 977
AgeI A'CCGG_T
Cuts at: 4934 4934
Size: 6103
AhdI GACnn_n'nnGTC
Cuts at: 2641 4832 2641
Size: 2191 3912
AleI CACnn'nnGTG
Cuts at: 1166 3144 1166
Size: 1978 4125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2150 1184
Size: 966 5137
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2748 2780 2748
Size: 32 6071
AlwNI CAG_nnn'CTG
Cuts at: 2332 2737 3846 5418 2332
Size: 405 1109 1572 3017
Fragments arranged by size:
3017 1572 1109 405
ApaLI G'TGCA_C
Cuts at: 4160 5316 4160
Size: 1156 4947
ApoI r'AATT_y
Cuts at: 1493 3412 3638 3790 5899 1493
Size: 1919 226 152 2109 1697
Fragments arranged by size:
2109 1919 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4657 160
Size: 872 3625 1606
Fragments arranged by size:
3625 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 2173 2228 2408 3428 1870
Size: 73 113 117 55 180 1020 4545
Fragments arranged by size:
4545 1020 180 117 113 73 55
BamHI G'GATC_C
Cuts at: 3433 3800 3433
Size: 367 5736
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 3424 4879 618
Size: 325 217 627 273 1364 1455 1842
Fragments arranged by size:
1842 1455 1364 627 325 273 217
BanII G_rGCy'C
Cuts at: 1391 2081 2409 3422 1391
Size: 690 328 1013 4072
Fragments arranged by size:
4072 1013 690 328
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2029 2063 4294 4328 1903
Size: 34 92 34 2231 34 3678
Fragments arranged by size:
3678 2231 92 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3995 5205 3995
Size: 1210 4893
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6103
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4714 136
Size: 107 122 71 631 605 3042 1525
Fragments arranged by size:
3042 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6098 6098
Size: 6103
BlpI GC'TnA_GC
Cuts at: 3520 3520
Size: 6103
Bme1580I G_kGCm'C
Cuts at: 1790 2063 2229 4164 5320 1790
Size: 273 166 1935 1156 2573
Fragments arranged by size:
2573 1935 1156 273 166
BmgBI CAC'GTC
Cuts at: 1318 2654 1318
Size: 1336 4767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3834 4792 442
Size: 416 53 83 399 24 2417 958 1753
Fragments arranged by size:
2417 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2065 2396 4763 1239
Size: 826 331 2367 2579
Fragments arranged by size:
2579 2367 826 331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2402 4228 5093 5391 2402
Size: 1826 865 298 3114
Fragments arranged by size:
3114 1826 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4766 882
Size: 617 3267 2219
Fragments arranged by size:
3267 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2095 3894 493
Size: 711 891 1799 2702
Fragments arranged by size:
2702 1799 891 711
BsaBI GATnn'nnATC
Cuts at: 6097 6097
Size: 6103
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2274 2925 3910 4292
Size: 53 83 186 936 741 651 985 382
Cuts at: 4292 4927 275
Size: 635 1451
Fragments arranged by size:
1451 985 936 741 651 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2913 2961 4536 4934 5208 5355 5766 2913
Size: 48 1575 398 274 147 411 3250
Fragments arranged by size:
3250 1575 411 398 274 147 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3245 3275 1178
Size: 30 165 30 1842 30 4006
Fragments arranged by size:
4006 1842 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2188 2471 2474 2591 3239 1186
Size: 1002 283 3 117 648 4050
Fragments arranged by size:
4050 1002 648 283 117 3
BseYI C'CCAG_C
Cuts at: 2141 5306 5802 2141
Size: 3165 496 2442
Fragments arranged by size:
3165 2442 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2993 1163
Size: 573 1257 4273
Fragments arranged by size:
4273 1257 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2075 3553 4314 5342 1540
Size: 275 186 74 1478 761 1028 2301
Fragments arranged by size:
2301 1478 1028 761 275 186 74
BsiHKAI G_wGCw'C
Cuts at: 2081 2409 4164 4249 5320 2081
Size: 328 1755 85 1071 2864
Fragments arranged by size:
2864 1755 1071 328 85
BsiWI C'GTAC_G
Cuts at: 2818 2818
Size: 6103
BsmI GAATG_Cn'
Cuts at: 3614 3707 3614
Size: 93 6010
BsmBI CGTCTCn'nnnn_
Cuts at: 2649 2649
Size: 6103
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2081 2229 2409 3422 4164 4249
Size: 399 273 18 148 180 1013 742 85
Cuts at: 4249 5320 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1013 742 399 273 180 148
85 18
BspEI T'CCGG_A
Cuts at: 5766 5766
Size: 6103
BspHI T'CATG_A
Cuts at: 3992 5722 3992
Size: 1730 4373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2473 2862 3168 3458 844
Size: 1629 389 306 290 3489
Fragments arranged by size:
3489 1629 389 306 290
BsrBI CCG'CTC
Cuts at: 1394 1823 3990 1394
Size: 429 2167 3507
Fragments arranged by size:
3507 2167 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4598 4772 65
Size: 1183 3350 174 1396
Fragments arranged by size:
3350 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2070 2478 4747 4934 1700
Size: 110 260 408 2269 187 2869
Fragments arranged by size:
2869 2269 408 260 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6103
BssHII G'CGCG_C
Cuts at: 1925 2130 1925
Size: 205 5898
BssSI C'ACGA_G
Cuts at: 4163 5175 4163
Size: 1012 5091
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6103
BstBI TT'CG_AA
Cuts at: 4907 4907
Size: 6103
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6103
BstYI r'GATC_y
Cuts at: 1972 2391 3433 3800 4185 4202 5654 5740 5752
Size: 419 1042 367 385 17 1452 86 12
Cuts at: 5752 6098 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1042 419 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3272 3272
Size: 6103
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5551
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5364
Fragments arranged by size:
5364 683 56
BtsI GCAGTG_nn'
Cuts at: 3619 4413 4433 3619
Size: 794 20 5289
Fragments arranged by size:
5289 794 20
ClaI AT'CG_AT
Cuts at: 1554 2772 1554
Size: 1218 4885
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6068
DraI TTT'AAA
Cuts at: 3407 3407
Size: 6103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5110 809
Size: 507 3794 1802
Fragments arranged by size:
3794 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2072 2268 2400
Size: 54 1425 50 275 18 242 196 132
Cuts at: 2400 2538 3091 3550 4439 6068 8
Size: 138 553 459 889 1629 43
Fragments arranged by size:
1629 1425 889 553 459 275 242 196
138 132 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2072 3550 1537
Size: 275 260 1478 4090
Fragments arranged by size:
4090 1478 275 260
EarI CTCTTCn'nnn_
Cuts at: 1353 2685 2715 3042 3252 4033 1353
Size: 1332 30 327 210 781 3423
Fragments arranged by size:
3423 1332 781 327 210 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4690 5064 5210 387
Size: 1064 138 3101 374 146 1280
Fragments arranged by size:
3101 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2079 2407 2079
Size: 328 5775
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 2396 2570 4166 4763 5550 5818
Size: 393 433 331 174 1596 597 787 268
Cuts at: 5818 1239
Size: 1524
Fragments arranged by size:
1596 1524 787 597 433 393 331 268
174
EcoNI CCTnn'n_nnAGG
Cuts at: 2038 2453 2540 2858 2900 2933 2038
Size: 415 87 318 42 33 5208
Fragments arranged by size:
5208 415 318 87 42 33
EcoO109I rG'GnC_Cy
Cuts at: 2446 2446
Size: 6103
EcoRI G'AATT_C
Cuts at: 3412 3790 3412
Size: 378 5725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 6103
FspI TGC'GCA
Cuts at: 4609 4609
Size: 6103
HaeII r_GCGC'y
Cuts at: 1678 5250 1678
Size: 3572 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2627 2659 3299 3331 3351
Size: 32 135 32 1055 32 640 32 20
Cuts at: 3351 3383 4744 4776 4818 4850 1373
Size: 32 1361 32 42 32 2626
Fragments arranged by size:
2626 1361 1055 640 135 42 32 32
32 32 32 32 32 20
HincII GTy'rAC
Cuts at: 669 1657 1938 2635 3447 3693 669
Size: 988 281 697 812 246 3079
Fragments arranged by size:
3079 988 812 697 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6103
HpaI GTT'AAC
Cuts at: 3693 3693
Size: 6103
KpnI G_GTAC'C
Cuts at: 3428 3428
Size: 6103
MfeI C'AATT_G
Cuts at: 3702 3702
Size: 6103
MluI A'CGCG_T
Cuts at: 5979 5979
Size: 6103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5217 5401 1305
Size: 3912 184 2007
Fragments arranged by size:
3912 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4281
Fragments arranged by size:
4281 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3144 3390 4061 4420 4579 518
Size: 648 1978 246 671 359 159 2042
Fragments arranged by size:
2042 1978 671 648 359 246 159
MspA1I CmG'CkG
Cuts at: 1606 3519 4196 5344 5589 1606
Size: 1913 677 1148 245 2120
Fragments arranged by size:
2120 1913 1148 677 245
NaeI GCC'GGC
Cuts at: 2072 2480 2072
Size: 408 5695
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5551
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6103
NgoMIV G'CCGG_C
Cuts at: 2070 2478 2070
Size: 408 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6103
NotI GC'GGCC_GC
Cuts at: 3550 3550
Size: 6103
NspI r_CATG'y
Cuts at: 1132 1229 2765 3461 5006 1132
Size: 97 1536 696 1545 2229
Fragments arranged by size:
2229 1545 1536 696 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6103
PciI A'CATG_T
Cuts at: 5002 5002
Size: 6103
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6103
PfoI T'CCnGG_A
Cuts at: 2373 2559 2730 2373
Size: 186 171 5746
Fragments arranged by size:
5746 186 171
PmeI GTTT'AAAC
Cuts at: 3407 3407
Size: 6103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4172 4204 5685 5717 4172
Size: 32 1481 32 4558
Fragments arranged by size:
4558 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2446 2446
Size: 6103
PshAI GACnn'nnGTC
Cuts at: 1613 2820 1613
Size: 1207 4896
PsiI TTA'TAA
Cuts at: 3673 3673
Size: 6103
PspXI vC'TCGA_Gb
Cuts at: 2408 2408
Size: 6103
PstI C_TGCA'G
Cuts at: 830 2247 2328 2742 2859 3102 3455 830
Size: 1417 81 414 117 243 353 3478
Fragments arranged by size:
3478 1417 414 353 243 117 81
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6103
SacI G_AGCT'C
Cuts at: 2081 2409 2081
Size: 328 5775
SalI G'TCGA_C
Cuts at: 1936 3445 1936
Size: 1509 4594
SbfI CC_TGCA'GG
Cuts at: 2247 2859 3102 3455 2247
Size: 612 243 353 4895
Fragments arranged by size:
4895 612 353 243
ScaI AGT'ACT
Cuts at: 4351 6063 4351
Size: 1712 4391
SexAI A'CCwGG_T
Cuts at: 2012 2012
Size: 6103
SfcI C'TryA_G
Cuts at: 826 1045 2116 2243 2324 2738 2855 3098 3202
Size: 219 1071 127 81 414 117 243 104
Cuts at: 3202 3451 4586 5267 5458 826
Size: 249 1135 681 191 1471
Fragments arranged by size:
1471 1135 1071 681 414 249 243 219
191 127 117 104 81
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6103
SmaI CCC'GGG
Cuts at: 1872 2058 2175 2230 3430 1872
Size: 186 117 55 1200 4545
Fragments arranged by size:
4545 1200 186 117 55
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2408 2417 4207 5108 5370 820
Size: 197 926 465 9 1790 901 262 1553
Fragments arranged by size:
1790 1553 926 901 465 262 197 9
SnaBI TAC'GTA
Cuts at: 493 3894 493
Size: 3401 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6103
SphI G_CATG'C
Cuts at: 1132 3461 1132
Size: 2329 3774
SrfI GCCC'GGGC
Cuts at: 2058 2058
Size: 6103
SspI AAT'ATT
Cuts at: 5 52 4027 5
Size: 47 3975 2081
Fragments arranged by size:
3975 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6103
StyI C'CwwG_G
Cuts at: 513 1065 2425 3542 513
Size: 552 1360 1117 3074
Fragments arranged by size:
3074 1360 1117 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4130 4147 4300 4485 4930 325
Size: 1255 2550 17 153 185 445 1498
Fragments arranged by size:
2550 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4349 6061 96
Size: 274 80 33 51 1443 2372 1712 138
Fragments arranged by size:
2372 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6063 6095 6063
Size: 32 6071
Tth111I GACn'n_nGTC
Cuts at: 1316 2101 1316
Size: 785 5318
XbaI T'CTAG_A
Cuts at: 3439 3439
Size: 6103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2314 3019 3086 2314
Size: 705 67 5331
Fragments arranged by size:
5331 705 67
XhoI C'TCGA_G
Cuts at: 1943 2408 1943
Size: 465 5638
XmaI C'CCGG_G
Cuts at: 1870 2056 2173 2228 3428 1870
Size: 186 117 55 1200 4545
Fragments arranged by size:
4545 1200 186 117 55
XmnI GAAnn'nnTTC
Cuts at: 4232 4232
Size: 6103
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3911 276
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BstXI BstZ17I DraIII EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PsrI
PvuII RsrII SacII SanDI SapI SfiI SfoI SwaI