################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AlwI AlwNI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI TspRI Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AfaI 351 GTAC GT^AC Alw44I 410 GTGCAC G^TGCAC ApaLI 410 GTGCAC G^TGCAC AvaI 52 CCCGAG C^[CT]CG[GA]G AvaII 135 GGTCC G^G[AT]CC BalI 615 TGGCCA TGG^CCA BanI 162 GGTGCC G^G[CT][GA]CC BanII 550 GAGCTC G[GA]GC[CT]^C BbvCI 138 CCTCAGC CCTCAGC(-5/-2) BfaI 595 CTAG C^TAG BsmFI 690 GTCCC GGGAC(10/14) BsoBI 52 CCCGAG C^[CT]CG[GA]G BspMI 624 GCAGGT ACCTGC(4/8) BsrDI 663 CATTGC GCAATG(2/0) BssSI 305 CACGAG CACGAG(-5/-1) BstEII 654 GGTGACC G^GT[ACGT]ACC BstF5I 93 GGATG GGATG(2/0) BstPI 654 GGTGACC G^GT[ACGT]ACC BstXI 83 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 135 GGTCC G^G[ACGT]CC EaeI 615 TGGCCA [CT]^GGCC[GA] EarI 374 CTCTTC CTCTTC(1/4) EcoO65I 654 GGTGACC G^GT[ACGT]ACC EcoT14I 276 CCTTGG C^C[AT][AT]GG FokI 93 GGATG GGATG(9/13) HincII 97 GTCAAC GT[CT]^[GA]AC HindII 97 GTCAAC GT[CT]^[GA]AC Ksp632I 374 CTCTTC CTCTTC(1/4) MaeI 595 CTAG C^TAG MaeII 349 ACGT A^CGT MluNI 615 TGGCCA TGG^CCA MscI 615 TGGCCA TGG^CCA PflMI 451 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 351 GTAC GT^AC SacI 550 GAGCTC GAGCT^C Sau96I 135 GGTCC G^G[ACGT]CC SfaNI 77 GCATC GCATC(5/9) StyI 276 CCTTGG C^C[AT][AT]GG Van91I 451 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 672 CCAGTTCCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 448 CCGC CCGC(-3/-1) 570 CCGC AcsI 189 GAATTT [GA]^AATT[CT] 369 AAATTC AocI 489 CCTTAGG CC^T[ACGT]AGG 517 CCTAAGG ApoI 189 GAATTT [GA]^AATT[CT] 369 AAATTC BcnI 36 CCGGG CC^[GC]GG 72 CCGGG BseRI 28 GAGGAG GAGGAG(10/8) 226 GAGGAG BsmAI 718 GAGAC GTCTC(1/5) 9 GTCTC Bsu36I 489 CCTTAGG CC^T[ACGT]AGG 517 CCTAAGG Eco81I 489 CCTTAGG CC^T[ACGT]AGG 517 CCTAAGG HphI 654 GGTGA GGTGA(8/7) 575 TCACC MwoI 534 GCTTACCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 543 GCTTAAAGAGC NciI 36 CCGGG CC^[GC]GG 72 CCGGG NspI 86 ACATGC [GA]CATG^[CT] 746 ACATGC SmlI 331 CTTGAG C^T[CT][GA]AG 360 CTTGAG TaqI 224 TCGA T^CGA 790 TCGA Tsp509I 190 AATT ^AATT 370 AATT TthHB8I 224 TCGA T^CGA 790 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AluI AspHI BbvI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HpaII ItaI MaeIII MboI MboII MnlI MseI MspI MvaI NdeII NlaIII NlaIV Sau3AI ScrFI Tru9I TseI Tsp45I