################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HapII Hin1I HindIII HpaII KasI KpnI KspI MluI MroI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### AclI 320 AACGTT AA^CGTT AflIII 909 ACATGT A^C[GA][CT]GT AocI 524 CCTTAGG CC^T[ACGT]AGG AspHI 94 GAGCTC G[AT]GC[AT]^C AspI 675 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 162 GGACC G^G[AT]CC BalI 815 TGGCCA TGG^CCA BanII 94 GAGCTC G[GA]GC[CT]^C BbsI 451 GAAGAC GAAGAC(2/6) BciVI 860 GTATCC GTATCC(6/5) BfaI 239 CTAG C^TAG BmyI 94 GAGCTC G[AGT]GC[ACT]^C BpmI 443 CTGGAG CTGGAG(16/14) BpuAI 451 GAAGAC GAAGAC(2/6) BsaAI 784 TACGTG [CT]AC^GT[GA] BsaBI 799 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 640 GTGCAG GTGCAG(16/14) BsiHKAI 94 GAGCTC G[AT]GC[AT]^C BsiYI 57 CCTGCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 57 CCTGCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 298 GAGAC GTCTC(1/5) BsmI 609 GAATGC GAATGC(1/-1) Bsp1286I 94 GAGCTC G[AGT]GC[ACT]^C BspHI 477 TCATGA T^CATGA BspLU11I 909 ACATGT A^CATGT BspMI 56 ACCTGC ACCTGC(4/8) BssKI 442 CCTGG ^CC[ACGT]GG BstAPI 793 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 998 CATCC GGATG(2/0) BstNI 442 CCTGG CC^[AT]GG BstXI 1001 CCAAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 524 CCTTAGG CC^T[ACGT]AGG Cac8I 96 GCTCGC GC[ACGT]^[ACGT]GC DraI 915 TTTAAA TTT^AAA EaeI 815 TGGCCA [CT]^GGCC[GA] Eco81I 524 CCTTAGG CC^T[ACGT]AGG EcoRII 442 CCTGG ^CC[AT]GG FokI 998 CATCC GGATG(9/13) HaeII 510 GGCGCT [GA]GCGC^[CT] HgaI 231 GACGC GACGC(5/10) HincII 355 GTTAAC GT[CT]^[GA]AC HindII 355 GTTAAC GT[CT]^[GA]AC HpaI 355 GTTAAC GTT^AAC HphI 563 TCACC GGTGA(8/7) MaeI 239 CTAG C^TAG MaeIII 847 GTCAC ^GT[ACGT]AC MamI 799 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 494 CAATTG C^AATTG MluNI 815 TGGCCA TGG^CCA MscI 815 TGGCCA TGG^CCA MslI 998 CATCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 494 CAATTG C^AATTG MvaI 442 CCTGG CC^[AT]GG PflFI 675 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 320 AACGTT AA^CGTT PvuII 37 CAGCTG CAG^CTG RcaI 477 TCATGA T^CATGA SacI 94 GAGCTC GAGCT^C ScrFI 442 CCTGG CC^[ACGT]GG SfcI 989 CTACAG C^T[GA][CT]AG SspI 77 AATATT AAT^ATT TaqI 184 TCGA T^CGA Tsp45I 847 GTCAC ^GT[GC]AC Tth111I 675 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 184 TCGA T^CGA XcmI 864 CCATGCCAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 568 AAATTC [GA]^AATT[CT] 763 AAATTC AfaI 411 GTAC GT^AC 1014 GTAC AlwI 21 GATCC GGATC(4/5) 339 GATCC ApoI 568 AAATTC [GA]^AATT[CT] 763 AAATTC BbvI 36 GCAGC GCAGC(8/12) 385 GCAGC BclI 402 TGATCA T^GATCA 540 TGATCA BsaJI 389 CCTTGG C^C[ACGT][ACGT]GG 832 CCTTGG BseRI 157 GAGGAG GAGGAG(10/8) 272 GAGGAG BstYI 20 AGATCC [GA]^GATC[CT] 338 AGATCC CfoI 511 GCGC GCG^C 829 GCGC Cfr13I 162 GGACC G^G[ACGT]CC 836 GGCCC EcoT14I 389 CCTTGG C^C[AT][AT]GG 832 CCTTGG FbaI 402 TGATCA T^GATCA 540 TGATCA HaeIII 816 GGCC GG^CC 836 GGCC HhaI 511 GCGC GCG^C 829 GCGC HinP1I 511 GCGC G^CGC 829 GCGC MaeII 321 ACGT A^CGT 785 ACGT MflI 20 AGATCC [GA]^GATC[CT] 338 AGATCC MspA1I 37 CAGCTG C[AC]G^C[GT]G 931 CAGCGG NlaIV 53 GGAACC GG[ACGT]^[ACGT]CC 438 GGAGCC NspI 900 GCATGT [GA]CATG^[CT] 909 ACATGT PleI 520 GAGTC GAGTC(4/5) 679 GAGTC RsaI 411 GTAC GT^AC 1014 GTAC Sau96I 162 GGACC G^G[ACGT]CC 836 GGCCC SfaNI 310 GATGC GCATC(5/9) 799 GATGC StyI 389 CCTTGG C^C[AT][AT]GG 832 CCTTGG TseI 36 GCAGC G^C[AT]GC 385 GCAGC XhoII 20 AGATCC [GA]^GATC[CT] 338 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AciI AluI BsrI DdeI DpnII EarI Fnu4HI HinfI ItaI Ksp632I MboI MboII MnlI MseI MwoI NdeII NlaIII Sau3AI TfiI Tru9I Tsp509I TspRI