################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI ApaI AscI AseI AsnI Asp718I AspI AviII AvrII BbeI BbrPI BcgI BglII BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiWI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CpoI DraI DrdI EcoO65I EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 98 CGCG CG^CG AcyI 1869 GACGCC G[GA]^CG[CT]C AflII 475 CTTAAG C^TTAAG AhdI 16 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2059 CCTCAGG CC^T[ACGT]AGG Asp700I 262 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 16 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 770 CTCGAG C^[CT]CG[GA]G BanI 1336 GGCACC G^G[CT][GA]CC BciVI 775 GTATCC GTATCC(6/5) BfrI 475 CTTAAG C^TTAAG BglI 1361 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 1869 GACGCC G[GA]^CG[CT]C BsaI 2380 GAGACC GGTCTC(1/5) BsiEI 807 CGGCCG CG[GA][CT]^CG BsmBI 203 GAGACG CGTCTC(1/5) BsmI 2531 GAATGC GAATGC(1/-1) BsoBI 770 CTCGAG C^[CT]CG[GA]G BspDI 1111 ATCGAT AT^CGAT BsrFI 805 ACCGGC [GA]^CCGG[CT] BstUI 98 CGCG CG^CG Bsu36I 2059 CCTCAGG CC^T[ACGT]AGG Cfr10I 805 ACCGGC [GA]^CCGG[CT] ClaI 1111 ATCGAT AT^CGAT DsaI 1819 CCACGG C^C[GA][CT]GG EagI 807 CGGCCG C^GGCCG Eam1105I 16 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 807 CGGCCG C^GGCCG Eco52I 807 CGGCCG C^GGCCG Eco81I 2059 CCTCAGG CC^T[ACGT]AGG EcoNI 2229 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Hin1I 1869 GACGCC G[GA]^CG[CT]C MfeI 1412 CAATTG C^AATTG MunI 1412 CAATTG C^AATTG MvnI 98 CGCG CG^CG NdeI 945 CATATG CA^TATG PaeR7I 770 CTCGAG C^TCGAG PshAI 2382 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 1527 GAAGAGC GCTCTTC(1/4) ScaI 1226 AGTACT AGT^ACT StuI 380 AGGCCT AGG^CCT XhoI 770 CTCGAG C^TCGAG XmnI 262 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AflIII 1576 ACGTGT A^C[GA][CT]GT 2085 ACGTGT Alw44I 623 GTGCAC G^TGCAC 1194 GTGCAC AlwNI 1418 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 2496 CAGTAACTG Aor51HI 1017 AGCGCT AGC^GCT 2258 AGCGCT ApaLI 623 GTGCAC G^TGCAC 1194 GTGCAC BalI 221 TGGCCA TGG^CCA 848 TGGCCA BamHI 1052 GGATCC G^GATCC 1828 GGATCC BbsI 13 GAAGAC GAAGAC(2/6) 1026 GAAGAC BbvCI 1670 CCTCAGC CCTCAGC(-5/-2) 2119 GCTGAGG BclI 818 TGATCA T^GATCA 1487 TGATCA BcnI 464 CCCGG CC^[GC]GG 1857 CCCGG BfaI 665 CTAG C^TAG 677 CTAG BlpI 1263 GCTCAGC GC^T[ACGT]AGC 1810 GCTCAGC Bpu1102I 1263 GCTCAGC GC^T[ACGT]AGC 1810 GCTCAGC BpuAI 13 GAAGAC GAAGAC(2/6) 1026 GAAGAC BspMI 1568 ACCTGC ACCTGC(4/8) 2383 ACCTGC BstAPI 1270 GCAAAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2345 GCAAGACGTGC BstXI 554 CCAAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 728 CCATCTGTGTGG CelII 1263 GCTCAGC GC^T[ACGT]AGC 1810 GCTCAGC DraIII 914 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG 977 CACAATGTG Eco47III 1017 AGCGCT AGC^GCT 2258 AGCGCT EcoRI 497 GAATTC G^AATTC 1310 GAATTC FbaI 818 TGATCA T^GATCA 1487 TGATCA HaeII 1017 AGCGCT [GA]GCGC^[CT] 2258 AGCGCT HincII 282 GTCAAC GT[CT]^[GA]AC 507 GTCAAC HindII 282 GTCAAC GT[CT]^[GA]AC 507 GTCAAC MaeI 665 CTAG C^TAG 677 CTAG MluNI 221 TGGCCA TGG^CCA 848 TGGCCA MscI 221 TGGCCA TGG^CCA 848 TGGCCA NciI 464 CCCGG CC^[GC]GG 1857 CCCGG PvuII 1421 CAGCTG CAG^CTG 1778 CAGCTG SacI 130 GAGCTC GAGCT^C 1755 GAGCTC XcmI 851 CCAGAGAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2409 CCACTGGCATGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII