Restriction map for
pFN21AB8520
(Circular) (Six-base) MAPSORT of: pFN21AB8520.seq Check: 3560 from: 1 to: 7327
pFN21AB8520 7327 bp
With 182 enzymes: SgfI *
May 26, 2011 14:10 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5137 278
Size: 53 83 186 936 3601 2468
Fragments arranged by size:
3601 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4670 1937
Size: 2733 4594
Acc65I G'GTAC_C
Cuts at: 4648 4648
Size: 7327
AclI AA'CG_TT
Cuts at: 1563 5454 5827 1563
Size: 3891 373 3063
Fragments arranged by size:
3891 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2929 3612 5390 6774 7042 1632
Size: 1297 683 1778 1384 268 1917
Fragments arranged by size:
1917 1778 1384 1297 683 268
AfeI AGC'GCT
Cuts at: 3024 4265 3024
Size: 1241 6086
AflII C'TTAA_G
Cuts at: 820 1017 2480 820
Size: 197 1463 5667
Fragments arranged by size:
5667 1463 197
AflIII A'CryG_T
Cuts at: 1203 3581 4090 6226 7203 1203
Size: 2378 509 2136 977 1327
Fragments arranged by size:
2378 2136 1327 977 509
AgeI A'CCGG_T
Cuts at: 6158 6158
Size: 7327
AhdI GACnn_n'nnGTC
Cuts at: 2026 6056 2026
Size: 4030 3297
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7327
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7327
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3897 3929 3897
Size: 32 7295
AlwNI CAG_nnn'CTG
Cuts at: 3428 4506 5070 6642 3428
Size: 1078 564 1572 4113
Fragments arranged by size:
4113 1572 1078 564
ApaLI G'TGCA_C
Cuts at: 2628 3199 5384 6540 2628
Size: 571 2185 1156 3415
Fragments arranged by size:
3415 2185 1156 571
ApoI r'AATT_y
Cuts at: 1493 2365 2502 3214 3315 4190 4607 4636 4862
Size: 872 137 712 101 875 417 29 226
Cuts at: 4862 5014 7123 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 875 872 712 417 226 152
137 101 29
AseI AT'TA_AT
Cuts at: 160 1032 5881 160
Size: 872 4849 1606
Fragments arranged by size:
4849 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7327
AvaI C'yCGr_G
Cuts at: 1870 1943 2775 4652 1870
Size: 73 832 1877 4545
Fragments arranged by size:
4545 1877 832 73
BamHI G'GATC_C
Cuts at: 3057 3833 4657 5024 3057
Size: 776 824 367 5360
Fragments arranged by size:
5360 824 776 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3341 4648 6103 618
Size: 325 217 627 1554 1307 1455 1842
Fragments arranged by size:
1842 1554 1455 1307 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2121 2139 3647 3764 3782 3862 4646 1391
Size: 730 18 1508 117 18 80 784 4072
Fragments arranged by size:
4072 1508 784 730 117 80 18 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2025 3038 928
Size: 971 126 1013 5217
Fragments arranged by size:
5217 1013 971 126
BbvCI CC'TCA_GC
Cuts at: 3676 4125 3676
Size: 449 6878
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5518 5552 1903
Size: 34 3581 34 3678
Fragments arranged by size:
3678 3581 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2791 5219 6429 2791
Size: 2428 1210 3689
Fragments arranged by size:
3689 2428 1210
BclI T'GATC_A
Cuts at: 1548 2823 3492 1548
Size: 1275 669 5383
Fragments arranged by size:
5383 1275 669
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3372 5938 136
Size: 107 122 71 631 605 1700 2566 1525
Fragments arranged by size:
2566 1700 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7322 7322
Size: 7327
BlpI GC'TnA_GC
Cuts at: 3269 3816 4744 3269
Size: 547 928 5852
Fragments arranged by size:
5852 928 547
Bme1580I G_kGCm'C
Cuts at: 1790 2632 3203 3485 5388 6544 1790
Size: 842 571 282 1903 1156 2573
Fragments arranged by size:
2573 1903 1156 842 571 282
BmgBI CAC'GTC
Cuts at: 1318 3582 4356 1318
Size: 2264 774 4289
Fragments arranged by size:
4289 2264 774
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5058 6016 442
Size: 416 53 83 399 24 3641 958 1753
Fragments arranged by size:
3641 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7327
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2586 2586
Size: 7327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2577 3327 3872 4097 4403 5987 1239
Size: 1338 750 545 225 306 1584 2579
Fragments arranged by size:
2579 1584 1338 750 545 306 225
Bpu10I CC'TnA_GC
Cuts at: 2765 3676 4125 2765
Size: 911 449 5967
Fragments arranged by size:
5967 911 449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3748 4104 5452 6317 6615 3748
Size: 356 1348 865 298 4460
Fragments arranged by size:
4460 1348 865 356 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4379 5990 882
Size: 617 2880 1611 2219
Fragments arranged by size:
2880 2219 1611 617
BsaAI yAC'GTr
Cuts at: 493 1204 5118 493
Size: 711 3914 2702
Fragments arranged by size:
3914 2702 711
BsaBI GATnn'nnATC
Cuts at: 7321 7321
Size: 7327
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3875 5134 5516 6151
Size: 53 83 186 936 2342 1259 382 635
Cuts at: 6151 275
Size: 1451
Fragments arranged by size:
2342 1451 1259 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5760 6158 6432 6579 6990 5760
Size: 398 274 147 411 6097
Fragments arranged by size:
6097 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3897 3927 1178
Size: 30 165 30 2494 30 4578
Fragments arranged by size:
4578 2494 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2503 3113 3566 3733 4584 4605 1186
Size: 841 476 610 453 167 851 21 3908
Fragments arranged by size:
3908 851 841 610 476 453 167 21
BseYI C'CCAG_C
Cuts at: 2434 3781 3883 6530 7026 2434
Size: 1347 102 2647 496 2735
Fragments arranged by size:
2735 2647 1347 496 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6754
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2815 4777 5538 6566 1540
Size: 275 186 814 1962 761 1028 2301
Fragments arranged by size:
2301 1962 1028 814 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2139 2632 3203 3764 5388 5473 6544 2139
Size: 493 571 561 1624 85 1071 2922
Fragments arranged by size:
2922 1624 1071 571 561 493 85
BsmI GAATG_Cn'
Cuts at: 4542 4838 4931 4542
Size: 296 93 6938
Fragments arranged by size:
6938 296 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2202 2202
Size: 7327
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2139 2632 3203 3485 3647 3764
Size: 399 331 18 493 571 282 162 117
Cuts at: 3764 3782 3862 4646 5388 5473 6544 1391
Size: 18 80 784 742 85 1071 2174
Fragments arranged by size:
2174 1071 784 742 571 493 399 331
282 162 117 85 80 18 18
BspEI T'CCGG_A
Cuts at: 6990 6990
Size: 7327
BspHI T'CATG_A
Cuts at: 5216 6946 5216
Size: 1730 5597
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3582 4397 4682 844
Size: 2738 815 285 3489
Fragments arranged by size:
3489 2738 815 285
BsrBI CCG'CTC
Cuts at: 1394 1823 5214 1394
Size: 429 3391 3507
Fragments arranged by size:
3507 3391 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5822 5996 65
Size: 1183 4574 174 1396
Fragments arranged by size:
4574 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2810 5971 6158 1700
Size: 110 1000 3161 187 2869
Fragments arranged by size:
3161 2869 1000 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7327
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7327
BssSI C'ACGA_G
Cuts at: 5387 6399 5387
Size: 1012 6315
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3281 4356 794
Size: 2487 1075 3765
Fragments arranged by size:
3765 2487 1075
BstBI TT'CG_AA
Cuts at: 6131 6131
Size: 7327
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7327
BstXI CCAn_nnnn'nTGG
Cuts at: 2566 2740 2566
Size: 174 7153
BstYI r'GATC_y
Cuts at: 1972 2596 3057 3286 3833 4657 5024 5409 5426
Size: 624 461 229 547 824 367 385 17
Cuts at: 5426 6878 6964 6976 7322 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 824 624 547 461 385 367
346 229 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4065 4065
Size: 7327
BtgI C'CryG_G
Cuts at: 513 1065 2004 3824 513
Size: 552 939 1820 4016
Fragments arranged by size:
4016 1820 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6588
Fragments arranged by size:
6588 683 56
BtsI GCAGTG_nn'
Cuts at: 2042 4495 4843 5637 5657 2042
Size: 2453 348 794 20 3712
Fragments arranged by size:
3712 2453 794 348 20
ClaI AT'CG_AT
Cuts at: 1554 3117 1554
Size: 1563 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2218 2253 2653 2688 529
Size: 35 1654 35 400 35 5168
Fragments arranged by size:
5168 1654 400 35 35 35
DraI TTT'AAA
Cuts at: 4631 4631
Size: 7327
DraIII CAC_nnn'GTG
Cuts at: 2924 2987 2924
Size: 63 7264
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6334 809
Size: 507 5018 1802
Fragments arranged by size:
5018 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2226 2471 2812
Size: 54 1425 50 275 18 396 245 341
Cuts at: 2812 2853 3864 4774 5663 7292 8
Size: 41 1011 910 889 1629 43
Fragments arranged by size:
1629 1425 1011 910 889 396 341 275
245 54 50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2812 4774 1537
Size: 275 1000 1962 4090
Fragments arranged by size:
4090 1962 1000 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2169 2337 3527 4427 5257 1353
Size: 816 168 1190 900 830 3423
Fragments arranged by size:
3423 1190 900 830 816 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5914 6288 6434 387
Size: 1064 138 4325 374 146 1280
Fragments arranged by size:
4325 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2137 3762 2137
Size: 1625 5702
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2577 2929 3327 3612 3872 4097 4403
Size: 393 945 352 398 285 260 225 306
Cuts at: 4403 5390 5987 6774 7042 1239
Size: 987 597 787 268 1524
Fragments arranged by size:
1524 987 945 787 597 398 393 352
306 285 268 260 225
EcoNI CCTnn'n_nnAGG
Cuts at: 4238 4238
Size: 7327
EcoO109I rG'GnC_Cy
Cuts at: 2302 2700 4060 2302
Size: 398 1360 5569
Fragments arranged by size:
5569 1360 398
EcoRI G'AATT_C
Cuts at: 2502 3315 4636 5014 2502
Size: 813 1321 378 4815
Fragments arranged by size:
4815 1321 813 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2628 2628
Size: 7327
FspI TGC'GCA
Cuts at: 5833 5833
Size: 7327
HaeII r_GCGC'y
Cuts at: 1678 3026 4267 6474 1678
Size: 1348 1241 2207 2531
Fragments arranged by size:
2531 2207 1348 1241
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 2147 2179 2641
Size: 32 135 32 440 32 103 32 462
Cuts at: 2641 2673 3278 3310 5968 6000 6042 6074 1373
Size: 32 605 32 2658 32 42 32 2626
Fragments arranged by size:
2658 2626 605 462 440 135 103 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2289 2514 4671 4917 669
Size: 988 281 351 225 2157 246 3079
Fragments arranged by size:
3079 2157 988 351 281 246 225
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7327
HpaI GTT'AAC
Cuts at: 4917 4917
Size: 7327
KpnI G_GTAC'C
Cuts at: 4652 4652
Size: 7327
MfeI C'AATT_G
Cuts at: 3417 4926 3417
Size: 1509 5818
MluI A'CGCG_T
Cuts at: 7203 7203
Size: 7327
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3690 6441 6625 1305
Size: 2385 2751 184 2007
Fragments arranged by size:
2751 2385 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2228 2855 10
Size: 54 1425 343 396 627 4482
Fragments arranged by size:
4482 1425 627 396 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2738 2792 4419 4425 5285 5644 5803
Size: 648 1572 54 1627 6 860 359 159
Cuts at: 5803 518
Size: 2042
Fragments arranged by size:
2042 1627 1572 860 648 359 159 54
6
MspA1I CmG'CkG
Cuts at: 1606 3428 3703 3785 4743 5420 6568 6813 1606
Size: 1822 275 82 958 677 1148 245 2120
Fragments arranged by size:
2120 1822 1148 958 677 275 245 82
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5836
Fragments arranged by size:
5836 939 552
NdeI CA'TA_TG
Cuts at: 387 2951 387
Size: 2564 4763
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7327
NotI GC'GGCC_GC
Cuts at: 4774 4774
Size: 7327
NspI r_CATG'y
Cuts at: 1132 1229 2520 4350 4604 4685 6230 1132
Size: 97 1291 1830 254 81 1545 2229
Fragments arranged by size:
2229 1830 1545 1291 254 97 81
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7327
PciI A'CATG_T
Cuts at: 6226 6226
Size: 7327
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7327
PfoI T'CCnGG_A
Cuts at: 3498 4074 3498
Size: 576 6751
PmeI GTTT'AAAC
Cuts at: 4631 4631
Size: 7327
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3897 3929 5396 5428 6909 6941 3897
Size: 32 1467 32 1481 32 4283
Fragments arranged by size:
4283 1481 1467 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2302 2700 4060 2302
Size: 398 1360 5569
Fragments arranged by size:
5569 1360 398
PshAI GACnn'nnGTC
Cuts at: 1613 4391 1613
Size: 2778 4549
PsiI TTA'TAA
Cuts at: 4897 4897
Size: 7327
PspXI vC'TCGA_Gb
Cuts at: 2775 2775
Size: 7327
PstI C_TGCA'G
Cuts at: 830 3294 3759 4502 4679 830
Size: 2464 465 743 177 3478
Fragments arranged by size:
3478 2464 743 465 177
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7327
PvuII CAG'CTG
Cuts at: 3428 3785 3428
Size: 357 6970
SacI G_AGCT'C
Cuts at: 2139 3764 2139
Size: 1625 5702
SalI G'TCGA_C
Cuts at: 1936 4669 1936
Size: 2733 4594
SanDI GG'GwC_CC
Cuts at: 2700 2700
Size: 7327
SapI GCTCTTCn'nnn_
Cuts at: 3527 3527
Size: 7327
SbfI CC_TGCA'GG
Cuts at: 4679 4679
Size: 7327
ScaI AGT'ACT
Cuts at: 3233 5575 7287 3233
Size: 2342 1712 3273
Fragments arranged by size:
3273 2342 1712
SfcI C'TryA_G
Cuts at: 826 1045 2645 3290 3755 4498 4675 5810 6491
Size: 219 1600 645 465 743 177 1135 681
Cuts at: 6491 6682 826
Size: 191 1471
Fragments arranged by size:
1600 1471 1135 743 681 645 465 219
191 177
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7327
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7327
SmaI CCC'GGG
Cuts at: 1872 4654 1872
Size: 2782 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2480 2775 3763 4119 5431 6332
Size: 197 926 537 295 988 356 1312 901
Cuts at: 6332 6594 820
Size: 262 1553
Fragments arranged by size:
1553 1312 988 926 901 537 356 295
262 197
SnaBI TAC'GTA
Cuts at: 493 5118 493
Size: 4625 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7327
SphI G_CATG'C
Cuts at: 1132 4685 1132
Size: 3553 3774
SspI AAT'ATT
Cuts at: 5 52 5251 5
Size: 47 5199 2081
Fragments arranged by size:
5199 2081 47
StuI AGG'CCT
Cuts at: 1517 2387 1517
Size: 870 6457
StyI C'CwwG_G
Cuts at: 513 1065 2004 2067 3084 4473 4766 513
Size: 552 939 63 1017 1389 293 3074
Fragments arranged by size:
3074 1389 1017 939 552 293 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2872 3134 5354 5371 5524 5709 6154
Size: 1255 1292 262 2220 17 153 185 445
Cuts at: 6154 325
Size: 1498
Fragments arranged by size:
2220 1498 1292 1255 445 262 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3231 3258 5573
Size: 274 80 33 51 1443 1254 27 2315
Cuts at: 5573 7285 96
Size: 1712 138
Fragments arranged by size:
2315 1712 1443 1254 274 138 80 51
33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2611 2643 7287 7319 2611
Size: 32 4644 32 2619
Fragments arranged by size:
4644 2619 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7327
XbaI T'CTAG_A
Cuts at: 4663 4663
Size: 7327
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2863 4421 2863
Size: 1558 5769
XhoI C'TCGA_G
Cuts at: 1943 2775 1943
Size: 832 6495
XmaI C'CCGG_G
Cuts at: 1870 4652 1870
Size: 2782 4545
XmnI GAAnn'nnTTC
Cuts at: 2271 5456 2271
Size: 3185 4142
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5135 276
Size: 53 83 186 936 3601 2468
Fragments arranged by size:
3601 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI