(Linear) (Six-base) MAPSORT of: KIBB8520.seq Check: 3689 from: 1 to: 2622 KIBB8520.orf With 182 enzymes: SgfI * May 26, 2011 13:34 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 924 1607 2622 Size: 924 683 1015 Fragments arranged by size: 1015 924 683 AfeI AGC'GCT Cuts at: 0 1019 2260 2622 Size: 1019 1241 362 Fragments arranged by size: 1241 1019 362 AflII C'TTAA_G Cuts at: 0 475 2622 Size: 475 2147 AflIII A'CryG_T Cuts at: 0 1576 2085 2622 Size: 1576 509 537 Fragments arranged by size: 1576 537 509 AhdI GACnn_n'nnGTC Cuts at: 0 21 2622 Size: 21 2601 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1892 1924 2622 Size: 1892 32 698 Fragments arranged by size: 1892 698 32 AlwNI CAG_nnn'CTG Cuts at: 0 1423 2501 2622 Size: 1423 1078 121 Fragments arranged by size: 1423 1078 121 ApaLI G'TGCA_C Cuts at: 0 623 1194 2622 Size: 623 571 1428 Fragments arranged by size: 1428 623 571 ApoI r'AATT_y Cuts at: 0 360 497 1209 1310 2185 2602 2622 Size: 360 137 712 101 875 417 20 Fragments arranged by size: 875 712 417 360 137 101 20 AvaI C'yCGr_G Cuts at: 0 770 2622 Size: 770 1852 BamHI G'GATC_C Cuts at: 0 1052 1828 2622 Size: 1052 776 794 Fragments arranged by size: 1052 794 776 BanI G'GyrC_C Cuts at: 0 1336 2622 Size: 1336 1286 BanII G_rGCy'C Cuts at: 0 116 134 1642 1759 1777 1857 2622 Size: 116 18 1508 117 18 80 765 Fragments arranged by size: 1508 765 117 116 80 18 18 BbsI GAAGACnn'nnnn_ Cuts at: 0 20 1033 2622 Size: 20 1013 1589 Fragments arranged by size: 1589 1013 20 BbvCI CC'TCA_GC Cuts at: 0 1671 2120 2622 Size: 1671 449 502 Fragments arranged by size: 1671 502 449 BciVI GTATCCnnnnn_n' Cuts at: 0 786 2622 Size: 786 1836 BclI T'GATC_A Cuts at: 0 818 1487 2622 Size: 818 669 1135 Fragments arranged by size: 1135 818 669 BglI GCCn_nnn'nGGC Cuts at: 0 1367 2622 Size: 1367 1255 BlpI GC'TnA_GC Cuts at: 0 1264 1811 2622 Size: 1264 547 811 Fragments arranged by size: 1264 811 547 Bme1580I G_kGCm'C Cuts at: 0 627 1198 1480 2622 Size: 627 571 282 1142 Fragments arranged by size: 1142 627 571 282 BmgBI CAC'GTC Cuts at: 0 1577 2351 2622 Size: 1577 774 271 Fragments arranged by size: 1577 774 271 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 581 2622 Size: 581 2041 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 572 1322 1867 2092 2398 2622 Size: 572 750 545 225 306 224 Fragments arranged by size: 750 572 545 306 225 224 Bpu10I CC'TnA_GC Cuts at: 0 760 1671 2120 2622 Size: 760 911 449 502 Fragments arranged by size: 911 760 502 449 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1743 2099 2622 Size: 1743 356 523 Fragments arranged by size: 1743 523 356 BsaI GGTCTCn'nnnn_ Cuts at: 0 2374 2622 Size: 2374 248 BsaHI Gr'CG_yC Cuts at: 0 1870 2622 Size: 1870 752 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1892 1922 2622 Size: 1892 30 700 Fragments arranged by size: 1892 700 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 22 498 1108 1561 1728 2579 2600 2622 Size: 22 476 610 453 167 851 21 22 Fragments arranged by size: 851 610 476 453 167 22 22 21 BseYI C'CCAG_C Cuts at: 0 429 1776 1878 2622 Size: 429 1347 102 744 Fragments arranged by size: 1347 744 429 102 BsiEI CG_ry'CG Cuts at: 0 810 2622 Size: 810 1812 BsiHKAI G_wGCw'C Cuts at: 0 134 627 1198 1759 2622 Size: 134 493 571 561 863 Fragments arranged by size: 863 571 561 493 134 BsmI GAATG_Cn' Cuts at: 0 2537 2622 Size: 2537 85 BsmBI CGTCTCn'nnnn_ Cuts at: 0 197 2622 Size: 197 2425 Bsp1286I G_dGCh'C Cuts at: 0 116 134 627 1198 1480 1642 1759 1777 Size: 116 18 493 571 282 162 117 18 Cuts at: 1777 1857 2622 Size: 80 765 Fragments arranged by size: 765 571 493 282 162 117 116 80 18 18 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1577 2392 2622 Size: 1577 815 230 Fragments arranged by size: 1577 815 230 BsrFI r'CCGG_y Cuts at: 0 805 2622 Size: 805 1817 BstAPI GCAn_nnn'nTGC Cuts at: 0 1276 2351 2622 Size: 1276 1075 271 Fragments arranged by size: 1276 1075 271 BstXI CCAn_nnnn'nTGG Cuts at: 0 561 735 2622 Size: 561 174 1887 Fragments arranged by size: 1887 561 174 BstYI r'GATC_y Cuts at: 0 591 1052 1281 1828 2622 Size: 591 461 229 547 794 Fragments arranged by size: 794 591 547 461 229 Bsu36I CC'TnA_GG Cuts at: 0 2060 2622 Size: 2060 562 BtgI C'CryG_G Cuts at: 0 1819 2622 Size: 1819 803 BtsI GCAGTG_nn' Cuts at: 0 37 2490 2622 Size: 37 2453 132 Fragments arranged by size: 2453 132 37 ClaI AT'CG_AT Cuts at: 0 1112 2622 Size: 1112 1510 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 213 248 648 683 2622 Size: 213 35 400 35 1939 Fragments arranged by size: 1939 400 213 35 35 DraIII CAC_nnn'GTG Cuts at: 0 919 982 2622 Size: 919 63 1640 Fragments arranged by size: 1640 919 63 EaeI y'GGCC_r Cuts at: 0 221 466 807 848 1859 2622 Size: 221 245 341 41 1011 763 Fragments arranged by size: 1011 763 341 245 221 41 EagI C'GGCC_G Cuts at: 0 807 2622 Size: 807 1815 EarI CTCTTCn'nnn_ Cuts at: 0 164 332 1522 2422 2622 Size: 164 168 1190 900 200 Fragments arranged by size: 1190 900 200 168 164 EcoICRI GAG'CTC Cuts at: 0 132 1757 2622 Size: 132 1625 865 Fragments arranged by size: 1625 865 132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 572 924 1322 1607 1867 2092 2398 2622 Size: 572 352 398 285 260 225 306 224 Fragments arranged by size: 572 398 352 306 285 260 225 224 EcoNI CCTnn'n_nnAGG Cuts at: 0 2233 2622 Size: 2233 389 EcoO109I rG'GnC_Cy Cuts at: 0 297 695 2055 2622 Size: 297 398 1360 567 Fragments arranged by size: 1360 567 398 297 EcoRI G'AATT_C Cuts at: 0 497 1310 2622 Size: 497 813 1312 Fragments arranged by size: 1312 813 497 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 623 2622 Size: 623 1999 HaeII r_GCGC'y Cuts at: 0 1021 2262 2622 Size: 1021 1241 360 Fragments arranged by size: 1241 1021 360 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 7 39 142 174 636 668 1273 1305 Size: 7 32 103 32 462 32 605 32 Cuts at: 1305 2622 Size: 1317 Fragments arranged by size: 1317 605 462 103 32 32 32 32 7 HincII GTy'rAC Cuts at: 0 284 509 2622 Size: 284 225 2113 Fragments arranged by size: 2113 284 225 MfeI C'AATT_G Cuts at: 0 1412 2622 Size: 1412 1210 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1685 2622 Size: 1685 937 MscI TGG'CCA Cuts at: 0 223 850 2622 Size: 223 627 1772 Fragments arranged by size: 1772 627 223 MslI CAynn'nnrTG Cuts at: 0 733 787 2414 2420 2622 Size: 733 54 1627 6 202 Fragments arranged by size: 1627 733 202 54 6 MspA1I CmG'CkG Cuts at: 0 1423 1698 1780 2622 Size: 1423 275 82 842 Fragments arranged by size: 1423 842 275 82 NdeI CA'TA_TG Cuts at: 0 946 2622 Size: 946 1676 NspI r_CATG'y Cuts at: 0 515 2345 2599 2622 Size: 515 1830 254 23 Fragments arranged by size: 1830 515 254 23 PfoI T'CCnGG_A Cuts at: 0 1493 2069 2622 Size: 1493 576 553 Fragments arranged by size: 1493 576 553 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1892 1924 2622 Size: 1892 32 698 Fragments arranged by size: 1892 698 32 PpuMI rG'GwC_Cy Cuts at: 0 297 695 2055 2622 Size: 297 398 1360 567 Fragments arranged by size: 1360 567 398 297 PshAI GACnn'nnGTC Cuts at: 0 2386 2622 Size: 2386 236 PspXI vC'TCGA_Gb Cuts at: 0 770 2622 Size: 770 1852 PstI C_TGCA'G Cuts at: 0 1289 1754 2497 2622 Size: 1289 465 743 125 Fragments arranged by size: 1289 743 465 125 PvuII CAG'CTG Cuts at: 0 1423 1780 2622 Size: 1423 357 842 Fragments arranged by size: 1423 842 357 SacI G_AGCT'C Cuts at: 0 134 1759 2622 Size: 134 1625 863 Fragments arranged by size: 1625 863 134 SanDI GG'GwC_CC Cuts at: 0 695 2622 Size: 695 1927 SapI GCTCTTCn'nnn_ Cuts at: 0 1522 2622 Size: 1522 1100 ScaI AGT'ACT Cuts at: 0 1228 2622 Size: 1228 1394 SfcI C'TryA_G Cuts at: 0 640 1285 1750 2493 2622 Size: 640 645 465 743 129 Fragments arranged by size: 743 645 640 465 129 SmlI C'TyrA_G Cuts at: 0 475 770 1758 2114 2622 Size: 475 295 988 356 508 Fragments arranged by size: 988 508 475 356 295 StuI AGG'CCT Cuts at: 0 382 2622 Size: 382 2240 StyI C'CwwG_G Cuts at: 0 62 1079 2468 2622 Size: 62 1017 1389 154 Fragments arranged by size: 1389 1017 154 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 867 1129 2622 Size: 867 262 1493 Fragments arranged by size: 1493 867 262 TatI w'GTAC_w Cuts at: 0 1226 1253 2622 Size: 1226 27 1369 Fragments arranged by size: 1369 1226 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 606 638 2622 Size: 606 32 1984 Fragments arranged by size: 1984 606 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 858 2416 2622 Size: 858 1558 206 Fragments arranged by size: 1558 858 206 XhoI C'TCGA_G Cuts at: 0 770 2622 Size: 770 1852 XmnI GAAnn'nnTTC Cuts at: 0 266 2622 Size: 266 2356 Enzymes that do cut: AcuI AfeI AflII AflIII AhdI AloI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII MfeI MmeI MscI MslI MspA1I NdeI NspI PfoI PpiI PpuMI PshAI PspXI PstI PvuII SacI SanDI SapI ScaI SfcI SmlI StuI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AgeI AleI AlfI ApaI AscI AseI AsiSI AvrII BaeI BbeI BcgI BfrBI BglII BmrI BmtI BsaAI BsaBI BsaWI BsgI BsiWI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI DraI DrdI EciI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PsiI PspOMI PsrI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XmaI ZraI