(Circular) (Six-base) MAPSORT of: pFN21AE4049.seq Check: 9272 from: 1 to: 5539 pFN21AE4049 5539 bp With 182 enzymes: SgfI * March 28, 2012 13:04 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2421 3349 278 Size: 53 83 186 936 885 928 2468 Fragments arranged by size: 2468 936 928 885 186 83 53 AccI GT'mk_AC Cuts at: 1937 2882 1937 Size: 945 4594 Acc65I G'GTAC_C Cuts at: 2860 2860 Size: 5539 AclI AA'CG_TT Cuts at: 1563 2838 3666 4039 1563 Size: 1275 828 373 3063 Fragments arranged by size: 3063 1275 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3602 4986 5254 1632 Size: 1970 1384 268 1917 Fragments arranged by size: 1970 1917 1384 268 AfeI AGC'GCT Cuts at: 2683 2683 Size: 5539 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5342 AflIII A'CryG_T Cuts at: 1203 2667 4438 5415 1203 Size: 1464 1771 977 1327 Fragments arranged by size: 1771 1464 1327 977 AgeI A'CCGG_T Cuts at: 4370 4370 Size: 5539 AhdI GACnn_n'nnGTC Cuts at: 4268 4268 Size: 5539 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5539 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5539 AlwNI CAG_nnn'CTG Cuts at: 2215 3282 4854 2215 Size: 1067 1572 2900 Fragments arranged by size: 2900 1572 1067 ApaI G_GGCC'C Cuts at: 2233 2233 Size: 5539 ApaLI G'TGCA_C Cuts at: 2072 3596 4752 2072 Size: 1524 1156 2859 Fragments arranged by size: 2859 1524 1156 ApoI r'AATT_y Cuts at: 1493 2848 3074 3226 5335 1493 Size: 1355 226 152 2109 1697 Fragments arranged by size: 2109 1697 1355 226 152 AseI AT'TA_AT Cuts at: 160 1032 4093 160 Size: 872 3061 1606 Fragments arranged by size: 3061 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2287 2481 2634 2864 1870 Size: 73 70 274 194 153 230 4545 Fragments arranged by size: 4545 274 230 194 153 73 70 BamHI G'GATC_C Cuts at: 2869 3236 2869 Size: 367 5172 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2129 2860 4315 618 Size: 325 217 627 342 731 1455 1842 Fragments arranged by size: 1842 1455 731 627 342 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2233 2858 1391 Size: 622 220 625 4072 Fragments arranged by size: 4072 625 622 220 BbeI G_GCGC'C Cuts at: 2133 2133 Size: 5539 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4568 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3730 3764 1903 Size: 34 1793 34 3678 Fragments arranged by size: 3678 1793 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3431 4641 3431 Size: 1210 4329 BclI T'GATC_A Cuts at: 1548 1548 Size: 5539 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4150 136 Size: 107 122 71 631 605 2478 1525 Fragments arranged by size: 2478 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5534 5534 Size: 5539 BlpI GC'TnA_GC Cuts at: 2956 2956 Size: 5539 Bme1580I G_kGCm'C Cuts at: 1790 2076 2233 2482 3600 4756 1790 Size: 286 157 249 1118 1156 2573 Fragments arranged by size: 2573 1156 1118 286 249 157 BmgBI CAC'GTC Cuts at: 1318 2044 1318 Size: 726 4813 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3270 4228 442 Size: 416 53 83 399 24 1853 958 1753 Fragments arranged by size: 1853 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5539 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2801 4199 1239 Size: 1562 1398 2579 Fragments arranged by size: 2579 1562 1398 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2773 3664 4529 4827 2773 Size: 891 865 298 3485 Fragments arranged by size: 3485 891 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4202 882 Size: 617 2703 2219 Fragments arranged by size: 2703 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2670 3330 493 Size: 711 1466 660 2702 Fragments arranged by size: 2702 1466 711 660 BsaBI GATnn'nnATC Cuts at: 2606 5533 2606 Size: 2927 2612 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2130 2418 2769 3346 Size: 53 83 186 936 597 288 351 577 Cuts at: 3346 3728 4363 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 597 577 382 351 288 186 83 53 BsaWI w'CCGG_w Cuts at: 3972 4370 4644 4791 5202 3972 Size: 398 274 147 411 4309 Fragments arranged by size: 4309 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5314 Fragments arranged by size: 5314 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5539 BseYI C'CCAG_C Cuts at: 2085 2225 4742 5238 2085 Size: 140 2517 496 2386 Fragments arranged by size: 2517 2386 496 140 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2156 2228 2351 2450 1163 Size: 573 420 72 123 99 4252 Fragments arranged by size: 4252 573 420 123 99 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2989 3750 4778 1540 Size: 275 186 988 761 1028 2301 Fragments arranged by size: 2301 1028 988 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2076 3600 3685 4756 2076 Size: 1524 85 1071 2859 Fragments arranged by size: 2859 1524 1071 85 BsmI GAATG_Cn' Cuts at: 2539 3050 3143 2539 Size: 511 93 4935 Fragments arranged by size: 4935 511 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2076 2233 2482 2858 3600 3685 Size: 399 223 63 157 249 376 742 85 Cuts at: 3685 4756 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 376 249 223 157 85 63 BspEI T'CCGG_A Cuts at: 5202 5202 Size: 5539 BspHI T'CATG_A Cuts at: 2548 3428 5158 2548 Size: 880 1730 2929 Fragments arranged by size: 2929 1730 880 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2894 844 Size: 2050 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2062 2127 2269 2385 3426 1394 Size: 429 239 65 142 116 1041 3507 Fragments arranged by size: 3507 1041 429 239 142 116 65 BsrDI GCAATG_nn' Cuts at: 65 1248 4034 4208 65 Size: 1183 2786 174 1396 Fragments arranged by size: 2786 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4183 4370 1700 Size: 110 2373 187 2869 Fragments arranged by size: 2869 2373 187 110 BsrGI T'GTAC_A Cuts at: 96 2349 96 Size: 2253 3286 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5539 BssSI C'ACGA_G Cuts at: 2075 2369 3599 4611 2075 Size: 294 1230 1012 3003 Fragments arranged by size: 3003 1230 1012 294 BstAPI GCAn_nnn'nTGC Cuts at: 794 2215 794 Size: 1421 4118 BstBI TT'CG_AA Cuts at: 4343 4343 Size: 5539 BstEII G'GTnAC_C Cuts at: 1184 2146 1184 Size: 962 4577 BstYI r'GATC_y Cuts at: 1972 2869 3236 3621 3638 5090 5176 5188 5534 Size: 897 367 385 17 1452 86 12 346 Cuts at: 5534 1972 Size: 1977 Fragments arranged by size: 1977 1452 897 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2151 2644 513 Size: 552 939 147 493 3408 Fragments arranged by size: 3408 939 552 493 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2289 487 Size: 683 56 1063 3737 Fragments arranged by size: 3737 1063 683 56 BtsI GCAGTG_nn' Cuts at: 3055 3849 3869 3055 Size: 794 20 4725 Fragments arranged by size: 4725 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5539 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5504 DraI TTT'AAA Cuts at: 2843 2843 Size: 5539 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4546 809 Size: 507 3230 1802 Fragments arranged by size: 3230 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2571 2986 3875 Size: 54 1425 50 275 18 741 415 889 Cuts at: 3875 5504 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 741 415 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2986 1537 Size: 275 1174 4090 Fragments arranged by size: 4090 1174 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3469 1353 Size: 2116 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2036 4126 4500 4646 387 Size: 1064 138 447 2090 374 146 1280 Fragments arranged by size: 2090 1280 1064 447 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2801 3602 4199 4986 5254 1239 Size: 393 1169 801 597 787 268 1524 Fragments arranged by size: 1524 1169 801 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2369 2369 Size: 5539 EcoO109I rG'GnC_Cy Cuts at: 2229 2457 2229 Size: 228 5311 EcoRI G'AATT_C Cuts at: 2848 3226 2848 Size: 378 5161 FspI TGC'GCA Cuts at: 4045 4045 Size: 5539 HaeII r_GCGC'y Cuts at: 1678 2133 2685 4686 1678 Size: 455 552 2001 2531 Fragments arranged by size: 2531 2001 552 455 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2813 2845 4180 4212 4254 Size: 32 135 32 1241 32 1335 32 42 Cuts at: 4254 4286 1373 Size: 32 2626 Fragments arranged by size: 2626 1335 1241 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2338 2590 2883 3129 669 Size: 988 281 400 252 293 246 3079 Fragments arranged by size: 3079 988 400 293 281 252 246 HindIII A'AGCT_T Cuts at: 748 2543 748 Size: 1795 3744 HpaI GTT'AAC Cuts at: 3129 3129 Size: 5539 KasI G'GCGC_C Cuts at: 2129 2129 Size: 5539 KpnI G_GTAC'C Cuts at: 2864 2864 Size: 5539 MfeI C'AATT_G Cuts at: 3138 3138 Size: 5539 MluI A'CGCG_T Cuts at: 5415 5415 Size: 5539 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4653 4837 1305 Size: 3348 184 2007 Fragments arranged by size: 3348 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3717 Fragments arranged by size: 3717 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2600 3497 3856 4015 518 Size: 648 1434 897 359 159 2042 Fragments arranged by size: 2042 1434 897 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 2107 2116 2212 2646 2955 3632 4780 Size: 483 18 9 96 434 309 677 1148 Cuts at: 4780 5025 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 483 434 309 245 96 18 9 NarI GG'CG_CC Cuts at: 2130 2130 Size: 5539 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4048 Fragments arranged by size: 4048 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5539 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5539 NotI GC'GGCC_GC Cuts at: 2986 2986 Size: 5539 NspI r_CATG'y Cuts at: 1132 1229 2897 4442 1132 Size: 97 1668 1545 2229 Fragments arranged by size: 2229 1668 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5539 PciI A'CATG_T Cuts at: 4438 4438 Size: 5539 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5539 PmeI GTTT'AAAC Cuts at: 2843 2843 Size: 5539 PmlI CAC'GTG Cuts at: 2670 2670 Size: 5539 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2374 2406 3608 3640 5121 5153 2374 Size: 32 1202 32 1481 32 2760 Fragments arranged by size: 2760 1481 1202 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2457 2457 Size: 5539 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5539 PsiI TTA'TAA Cuts at: 3109 3109 Size: 5539 PspOMI G'GGCC_C Cuts at: 2229 2229 Size: 5539 PstI C_TGCA'G Cuts at: 830 2106 2190 2744 2891 830 Size: 1276 84 554 147 3478 Fragments arranged by size: 3478 1276 554 147 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5539 PvuII CAG'CTG Cuts at: 2107 2116 2212 2107 Size: 9 96 5434 Fragments arranged by size: 5434 96 9 SacII CC_GC'GG Cuts at: 2647 2647 Size: 5539 SalI G'TCGA_C Cuts at: 1936 2881 1936 Size: 945 4594 SanDI GG'GwC_CC Cuts at: 2457 2457 Size: 5539 SbfI CC_TGCA'GG Cuts at: 2891 2891 Size: 5539 ScaI AGT'ACT Cuts at: 2705 3787 5499 2705 Size: 1082 1712 2745 Fragments arranged by size: 2745 1712 1082 SexAI A'CCwGG_T Cuts at: 2142 2142 Size: 5539 SfcI C'TryA_G Cuts at: 826 1045 2102 2186 2740 2887 4022 4703 4894 Size: 219 1057 84 554 147 1135 681 191 Cuts at: 4894 826 Size: 1471 Fragments arranged by size: 1471 1135 1057 681 554 219 191 147 84 SfoI GGC'GCC Cuts at: 2131 2131 Size: 5539 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5539 SmaI CCC'GGG Cuts at: 1872 2015 2483 2636 2866 1872 Size: 143 468 153 230 4545 Fragments arranged by size: 4545 468 230 153 143 SmlI C'TyrA_G Cuts at: 820 1017 1943 2752 3643 4544 4806 820 Size: 197 926 809 891 901 262 1553 Fragments arranged by size: 1553 926 901 891 809 262 197 SnaBI TAC'GTA Cuts at: 493 3330 493 Size: 2837 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5539 SphI G_CATG'C Cuts at: 1132 2897 1132 Size: 1765 3774 SspI AAT'ATT Cuts at: 5 52 3463 5 Size: 47 3411 2081 Fragments arranged by size: 3411 2081 47 StuI AGG'CCT Cuts at: 1517 2375 1517 Size: 858 4681 StyI C'CwwG_G Cuts at: 513 1065 2004 2331 2574 2978 513 Size: 552 939 327 243 404 3074 Fragments arranged by size: 3074 939 552 404 327 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2044 3566 3583 3736 3921 4366 325 Size: 1255 464 1522 17 153 185 445 1498 Fragments arranged by size: 1522 1498 1255 464 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2349 2703 3785 Size: 274 80 33 51 1443 372 354 1082 Cuts at: 3785 5497 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1082 372 354 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5499 5531 5499 Size: 32 5507 Tth111I GACn'n_nGTC Cuts at: 1316 2498 2728 1316 Size: 1182 230 4127 Fragments arranged by size: 4127 1182 230 XbaI T'CTAG_A Cuts at: 2875 2875 Size: 5539 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5539 XmaI C'CCGG_G Cuts at: 1870 2013 2481 2634 2864 1870 Size: 143 468 153 230 4545 Fragments arranged by size: 4545 468 230 153 143 XmnI GAAnn'nnTTC Cuts at: 3668 3668 Size: 5539 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2419 3347 276 Size: 53 83 186 936 885 928 2468 Fragments arranged by size: 2468 936 928 885 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SapI SfiI SrfI SwaI XcmI